체중감량 프로그램에 따른 체력의 변화에 대한 연구 : by Obese Female College Students in Seoul

박효정,장도석,장은재 동덕여자대학교 2003 생활과학연구 Vol.8 No.-

The purpose of this study was to investigate the effect of the aerobic exercise with calorie restricted diet on the change of the body composition, health-related fitness and performance-related fitness during 4 weeks. Twelve obese female college students ages from 20 to 24 who participated in this program. Variables were body composition; Fat mass, Fat free mass. Fat percentage, PIBW(Percentage of ideal Body Weight), BMI(Body Mass Index), health-related fitness and performance-related fitness. The results were as following; First. Diet was found to have a significant decrease of 266㎉ in the change of energy intake and exercise was found to have a significant consume of 309.71㎉ in the change of energy expenditure. Second, Weight, BMI, PIBW, percent body fat, fat mass were found to have a significant decrease of 5.02%, 5.01%, 5.25%, 2.58%, and 2.61% in the change of the body composition, respectively. (P>0.05) FFM was also decreased but it was not significantly. Third, Grip strength and Sit-ups were found to have a significant increase of 2.69% and 2.78% in the change of health-related fitness. (P>0.05) Back strength, Sitting trunk flexion and VO2max were found to have increase of 1.47%, 0.35% and 0.75% in the change of health-related fitness. Forth, Whole body reaction time was found to have a significant decrease of 2.62% in the change of performance-related fitness. Vertical jump and Closed eyes-foot balance were found to have a increase of 1.96% and 1.79% in the change of performance-related fitness. As the results, the weight control program demonstrated to have a positive effect in the improvement of the body composition, health-related fitness and performance-related fitness in obese female.

Population Changes of Moths (Insecta: Lepidoptera) from Mt. Wolchul National Park, Jeollanam-do, Korea

Choi, Sei-Woong,Park, Marana,Chang, Young-Hyo The Ecological Society of Korea 2007 Journal of Ecology and Environment Vol.30 No.3

We investigated the moth fauna and monthly changes in moth populations at three sites - Dogapsa, Gyungpodae and Muwisa - in Mt. Wolchul National Park, Jeollanam-do, South Korea. From February to October, 2006, we collected a total of 1677 individuals comprising 348 species in 14 families. Monthly changes in the abundance of species and individuals showed an M-shaped pattern, with the highest peak in June and a second high peak in August. The diversity of the three dominant families (Noctuidae, Geometridae and Pyralidae) at the three sites varied, possibly due to differences in vegetation and other environmental factors. Diversity at Dogapsa was relatively higher than Gyungpodae and Muwisa, but, the fauna at Dogapsa more closely resembled Muwisa than Gyungpodae. 28 species occurred at the same time in all three sites, included the families Geometridae (14 species), Noctuidae (9 species), Pyralidae (2 species), Arctiidae (1 species), Nolidae (1 species), and Limacodidae (1 species). The present study provided baseline information about biodiversity and phenological patterns of moth abundance and permitted evaluation of moth biodiversity as a monitoring tool for vegetation structure and environmental change.

연구논문 : 북한 미디어에 나타난 기후변화 프레임: 로동신문과 민주조선을 중심으로

박미선 ( Mi Sun Park ),윤여창 ( Yeo Chang Youn ),이효원 ( Hyo Won Lee ) 한국환경정책학회 2013 環境政策 Vol.21 No.4

Media define and interpret problems and recommend the treatment to them in environmental communication. This function of media is named as ``framing``. This paper aims to investigate how the issue of climate change as a global environmental issue has been communicated at the nationalmedia, using the theory ofmedia frame. In particular it focused on the case of North Korea. For media analysis, ‘Rodong Shinmun’ and ‘MinjuChosun’ as twomajor newspaperswere selected. The articles carrying terms of ‘climate change’ and ‘global warming’ in the title of articles were selected from1990 to 2012. The published data, places of events, cited speakers, different interest groups as victims, causers and problem solvers were coded and analyzed. Consequently the research results show four features of media frames on climate change in the North Korea. Frist, the selected articles includedmore foreign events and phenomena than the domestic. Second, governmental agencies, politicians, international organizations and scientists were represented as major speakers in the selected articles. In particular, instructions by Kim Il Sung and Kim Jong Il occupied the half of the statements by domestic speakers. Third, the diagnostic frame with description of victims and causers was dominant in the selected articles. Since 2007 the prognostic frame with representation of problem solvers was distinctly formed. Fourth, Rhodong Shinmun represented more various voices of the actors and interest groups as victims, causers and helpers to the problemof climate change thanMinju Chosun.

급성 췌장염으로 발현된 낭성 변화를 일으킨 종격동 부갑상선 선종 1예

권태현,박진흥,윤설영,이창렬,이준호,최소진,손성표,허갑도,최영식,박영효 성분도병원 1998 성분도병원논문집 Vol.4 No.-

급성 췌장염으로 발현된 낭성 변화를 이릉킨 종격동 부갑상선 선종 1예

이준호,최영식,박진홍,허갑도,윤설영,최소진,권태헌,이창렬,손성표,박영효 대한내분비학회 1998 Endocrinology and metabolism Vol.13 No.3

Hypercalcemia due to hyperparathyroidism is rarely associated with acute pancreatitis. But, the relationship between hypercalcemia and pancreatitis still remains controvesial. Ectopic parathyroid adenoma with cystic change is one of the rare causes of hyperparathyroidism, and is usually located in neck and mediastinum. We report a case of mediastinal parathyroid adenoma with cystic change associated with acute pancreatitis. A 54-year-old male presented with epigastric pain for 3 days. The serum calcium, phosphate, elastase were 16.8mg/dL, 1.1 mg/dL, 2772.0 ng/mL respectively and his parathyroid hormone level in serum was 651.84 pg/mL. Chest CT showed a mediastinal mass with well defined inhomogenously enhanced density located between SVC and aorta. The patient was diagnosed to hyperparathyroidism with acute pancreatitis due to mediastinal parathyroid adenoma with cystic change, and was surgically removed. The surgical biopsy showed parathyroid adenoma with cystic change. After operation his general condition was improved and serum calcium, phosphate, amylase, lipase level were normalized (J Kor Soc Endocrinol 13:473-479, 1998).

SV40 T 항원의 온도조건부 변이형 유전자가 포함된 Amphotropic Retrovirus 에 의한 사람 태아 간세포의 불멸화

이정일(Joung Il Lee),동석호(Seok Ho Dong),김효종(Hyo Jong Kim),김병호(Byung Ho Kim),장영운(Young Woon Chang),장린(Rin Chang),성세라(Se Ra Seong),박재경(Jae Kyung Park),김승보(Seung Bo Kim),이상목(Sang Mok Lee) 대한내과학회 1999 대한내과학회지 Vol.57 No.1

N/A Human cells are almost never spontaneously immortalized in vitro. We tried to immortalize human fetal hepatocytes (h-FH) and evaluate the differentiational status and its change. Methods : Hepatocytes were isolated from a liver fragment of 20 week old fetus and infected with amphotropic recombinant retrovirus containing a temperature- sensitive mutant of SV40 large T antigen and neomycin phosphotransferase gene. G418 resistant colonies were cloned and expanded. The cells which were able to divide more than 30 times were used to analyze various functions. Results : The immortalization rate was 3.3 x 10-8 and two cell lines (C11, D21) were established. C11-60, C11-80, D21-30 and D21-60 (suffix number means the cell division counts) were evaluated. D21-30 was thougt to be imcompletely immortalized because a considerable portion of cells died during culture. The morphology was similar to that of epithelial cells except for D21-30 which looked like fibroblast. The cells grew rapidly at 33oC but stopped growing at 39oC. T antigen and p53 was expressed at 33oC but disappeared at 39oC, which suggest that T antigen binds to p53. Chromosomal changes were so marked that it was impossible to discriminate exact number. Albumin was secreted as about 1/10 as that of h-FH, but alpha-fetoprotein secretion stopped after immortalization. Telomerase was activated in both cell lines except for the incompletely immortalized cells D21-30. Telomere was elongated in competely immortalized cell lines, but it was rather shortened in D21-30 compared to that of h-FH. Macroscopic colonies did not develop in soft agar assay. Conclusions : We successfully immortalized human fetal hepatocytes. Although the cells are not likely to have oncogenicity, the functions are not so good, possibly due to marked chromosomal changes which are thought to occur before telomerase is activated during immortalization step.

Ciprofloxacin의 동물유래 병원세균에 대한 시험관내 항균효과시험

장경수,장치훈,김일택,박승춘,윤효인,전무형 충남대학교 수의과대학 동물의과학연구소 1996 動物醫科學硏究誌 Vol.4 No.-

In this study the antimicrobial spectrum and sensitivity of ciprofloxacin(CFX) against the major pathogenic bacteria isolated from the diseased poultry, pig, cattle in Korea were evaluated in comparison with morfloxacin(NFX), enrofloxacin(EFX), nalidixic acid(NA), gentamicin(GM), tetracycline(TC), erythromycin(EM), streptomycin(SM) and penicillin(PC). Increasing by paper disk diffusion test for total of 439 isolates from poultry, pig and cattle, CFX showed remarkably higher sensitivity(>83%) as compared with other drugs. When three quinolones such as CFX, NFX and NA were compared for the inhibition activity against 4 major pathogens, CFX induced significantly larger diameter of inhibition zone through all of the tested concentration than NFX and NA. As MICs of all drugs for 11 bacterial species isolated from poultry were measured, the MIC range of CFX was 0.125 - 40 ㎍/㎖ in Gram positives, ≤ 0.005 - 2.5 ㎍/㎖ in Gram negatives and 0.08 - 1.25 ㎍/㎖ with 12 isolates of Mycoplasma. MIC_50 and MIC_90 of CFX in these cases revealed consistently lower as compared with those of NFX, GM and PC. When the MICs of the drugs were tested with 11 bacterial species from the diseased pigs, MIC range of CFX was 0.05 - 3.5 ㎍/㎖ in Gram positives, ≤ 0.005 - 0.8 ㎍/㎖ with Gram negatives and 0.5 - 9.0 ㎍/㎖ with 11 isolates of Mycoplasma. MIC_50 and MIC_90 of CFX and NFX were remarkably lower than those of GM and PC. As MICs of CFX, NFX, GM and PC for 10 bacterial species isolated from cattle were tested, the MIC range of CFX was found 0.01 - 4.0 ㎍/㎖ in Gram positives, and ≤ 0.005 - 0.2 ㎍/㎖ in Gram negative bacteria. The MIC_50 and MIC_90 of CFX in cases of cattle appeared remarkably lower than those of the other drugs. CFX, as compared with the other drugs, showed invariably lower MIC_50 and MIC_90 in both of GRam positives and negatives. As MICs of CFX, NFX and PC for 12 reference bacteria were examined, MIC of CFX was 0.03 - 0.25 ㎍/㎖ that was much lower drug concentration than those of NFX(0.25 - 1.0 ㎍/㎖) and PC(0.5 - 16.0㎍/㎖).

마이크로 압전 엑츄에이터용 Sol-Gel PZT 박막의 두께 변화에 따른 특성

장연태,박준식,김대식,박효덕,최승철 호서대학교 반도체제조장비국산화연구센터 2001 반도체장비학술심포지움 Vol.2001 No.-

Pb가 10% 과잉되고 Zr : Ti = 52 : 48 조성을 갖는 PZT sol이Pt(3500A˚)/Ti(400A˚)/SiO2(3000A˚)/Si(525㎛)기판 위에 스핀 코팅법으로 반복 코팅된 후, 450℃에서 10분, 650℃에서 2분간 반복 열처리되었다.이와 같이 다양한 두께로 적층된 박막은 각 시편에 대해 최종적으로 650℃ 30분 동안 어닐링 처리되었다.제조된 PZT 박막의 두께는 4100A*에서 1.75㎛사이의 4종이었다.이어서 스퍼터링법으로 Pt전극이 PZT 막 위에 증착되었다.제조된 PZT 박막의 결정 구조 조사를 위해 XRD, 그리고 미세 구조 및 전기적 특성을 알아보기 위해 FESEM과 P-E 이력 곡선이 각각 관찰되었다.4100A˚에서 1.75㎛까지 두께 중가에 따른 장비상의 포화 이력 한계로 잔류 분극(Pr)값이 25A˚u C/㎠에서 다소 감소되었다.측정된 X선 회절 결과에서 최초 4회 코팅시 perovskite 결정 구조로 성장한 결정립은 (111)배향이 우세하였으나, 두께가 증가됨에 따라 (111)/(110)값이 감소되었으며, 이를 통해 두께 증가에 따른 (111)배향성이 다소 감소됨을 알 수 있었다.이상의 결과로부터 제조된 PZT 박막은 큰 힘과 높은 내전압 특성을 갖는 마이크로 압전 액츄에이터에 적용될 수 있을 것으로 생각되었다.

제조합균주 E. coli Ck1092가 생산하는 2,3-Dihydroxybiphenyl Dioxygenase의 정제 및 특성

박효남,김영수,김영창,김치경,임재윤 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.3

4-CB 분해균주인 Pseudomonas sp. P20으로부터 pcbC 유전자를 클로닝하여 얻은 E. coli CK1092로부터 2,3-DHBP dioxygenase을 분리, 정제하여 효소적 특성을 조사하였다. 효소의 정제는 acetone 침전, DEAE-Se-phadex A-25 ion exchange chromatography, preparative electrophoresis 방법으로 정제하였다. 2,3-DHBP dioxygenase의 분자량은 약 270 kDa으로 추정되며, SDS-PAGE에 의한 분자량은 34 kDa이였다. 따라서, 동일한 subunit 8개 존재하는 octamer로 추정된다. 이 효소는 2,3-DHBP에 대해 높은 기질특이성을 보였으며, 3-methylcatechol, 4-methylcatechol, 4-chlorocatechol에 대해서는 활성을 보이지 않았다. 2,3-DHBP에 대한 Km 값은 18 μM이였으며 30μM 이상의 기질농도에서 활성이 감소하였다. 효소활성의 최적 pH는 8.0이였으며, pH 7.0~10.0 범위에서는 안정하였고, 최적 활성 온도는 40~60℃이며, 60℃까지는 비교적 안정하였다. 또한, 이 효소는 Cu^2+, Fe^2+, Fe^3+ 이온들에 의하여 효소활성이 저해되었고, H_2O_2와 EDTA에 의해서도 활성이 저해되었으며, 10%의 유기용매에 의해서 안정화되지 않았다. 효소활성부위를 알아보기 위해 화학변형제를 처리해 본 결과 tryosine, tryptophan과 histidine이 효소활성에 관여할 것으로 추정된다. 2,3-DHBP dioxygenase was purified from E. coli CK1092 carrying the pcbC gene, which was cloned from 4-chlorobiphenyl-degrading Pseudomonas sp. P20. Purification of this enzyme was done by acetone precipitation, DEAE-Sephadex A-25 ion exchange chromatography, and preparative gel electrophoresis. The molecular weight of subunit was 34 kDa determined by SDS-PAGE, and that of native enzyme was about 270 kDa. It suggests that this enzyme consist of eight identical subunits. This enzyme was specifically active against only 2,3-DHBP as a substrate with 18 μM of Km value, but not catechol, 3-methylcatechol, 4-methylcatechol and 4-chlorocatechol. The optimal pH and temperature of 2,3-DHBP dioxygenase were pH 8.0 and 40~60℃. The enzyme was inhibited by Cu^2+, Fe^2+ and Fe^3+ ions, and was inactivated by H_2O_2 and EDTA. The lower concentrations of some organic solvents such as acetone and ethanol don't stabilize the activity of 2,3-DHBP dioxygenase. The enzyme was completely inactivated by adding the reagents such as N-bromosuccinimide, iodine and p-diazobenzene sulfonic acid.

간경변증 환자에서 Escherichia coli 균혈증 합병 시 간기능 장애에 따른 C-reactive protein 생성 능력에의 영향

박완범,강철인,김동민,이기덕,장희창,김홍빈,오명돈,이효석,최강원 대한감염학회 2003 감염과 화학요법 Vol.35 No.5

목적 : C-reactive protein(CRP)은 간에서 생성되는 급성 반응물질이다. 하지만 간부전증 환자에서 CRP의 반응이 간기능에 따라 어느 정도 영향을 받는지는 별로 알려진 바가 없다. 본 연구에서는 간기능에 따른 CRP 생성 능력을 평가하고자 하였다. 방법 : E. coli 균혈증이 있는 간경변증 환자 30명을 대상으로 하였고 간기능은 균혈증이 발생하기 전 2개월 이내에 측정된 혈청 빌리루빈, 혈청 알부민, 프로트롬빈시간, Child-Pugh 점수로 평가하였다. 대조군 A는 간질환이 없으면서 E. coli 균혈증이 발생한 환자 30명으로 하였고 대조군 B는 간경변증이 있으면서 급성 감염의 증거가 없는 환자 30명으로 하였다. 환자군과 대조군 간에 CRP의 최대값을 비교하였다. 결과 : CRP의 최대값은 환자군에서 7.3±5.0㎎/dL, 대조군 A에서 17.9±8.3㎎/dL로 환자군에서 유의하게 낮았다.(P<0.001). 간경변증 환자에서 CRP의 생성은 Child-Pugh 점수에 비례하여 감소하였으나(P=0.004) Child=Pugh class C의 간기능을 가진 환자군에서도 대조군 B와 비교하여 의미있는 CRP의 생성을 보였다(5.3±3.2 vs. 0.5±0.4㎎/dL, P<0.001). 결론 : 간기능부전 환자에서 CRP 반응은 간기능 저하 정도에 따라 둔화되지만 심한 간기능 장애를 가진 환자에서도 CRP의 생성은 유지된다. Background : C-reactive protein (CRP) is an acute phase reactant produced in the liver. To assess the influence of liver dysfunction on the production of CRP, we evaluated CRP response to E. coli bacteremia in patients with or without liver cirrhosis (LC). Methods : 30 LC patients who developed spontaneous peritonitis with E. coli bacteremia were enrolled in the study. Baseline values of total bilirubin, serum albumin, and prothrombin time were obtained within 2 months prior to infection. Liver dysfunction was categorized according to the Child-Pugh score. 30 patients with E. coli bacteremia who had no underlying liver dysfunction were included as a control group. Matched-control of 30 LC patients without evidence of acute infection was also included. The peak CRP values were compared among the groups. Results : In the patients with E. coli bacteremia, the mean value of peak CRP was 7.3 (+/- 5.0) ㎎/dL in LC patients, 17.9 (+/- 8.3) mg/dL in patients without liver dysfunction (p<0.001). In the advanced LC patients with Child-Pugh class C, the level of CRP was 5.2 (+/- 3.3) ㎎/dL in patients with E. coli bacteremia, 0.5 (+/- 0.4) ㎎/dL in patients without acute infection (P<0.001). Child-Pugh score had correlation with decrease of CRP (linear regression test, P=0.004). Conclusion : CRP response during E. coli bacteremia was attenuated but maintained even in patients with advanced liver dysfunction.