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The Fine Functional Mapping of Penicillin G Acylase Gene
김영창,강현삼,오상진,송영환 한국유전학회 1982 Genes & Genomics Vol.4 No.2
Escherichia coli ATCC11105로부터 penicillin G acylase (PGA) 유전자를 plasmid pBR300에 cloning하여 얻은 pPAKS-1 및 그 유도체들에 있어서 PGA 유전자의 위치를 정확히 결정하기 위하여 우선 제한 효소 Eco RI, BamHI, Pst I, Sal I, Pvu II, Bgl I, Hind III, HinC II, Alu I, Kpn I, Hpa I, Xho I, Sau3A I 등을 이용하여 double digestion 또는 end-labelled DNA의 Partial digestion에 의해 제한 효소지도를 작성하였다. 이 제한효소지도를 기초로 하여 각종 제한 효소로 유발시킨 deletion mutant들의 제한효소절편과 효소역가를 상호 조사 비교함으로써 유전자의 위치를 결정할 수 있었기에 보고하는 바이다.
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Lactobacillus casei Bacperiophage의 분류 및 특성에 관한 연구
김영창,박민철,강국희,윤영호,이광웅 한국미생물학회 1979 미생물학회지 Vol.17 No.4
Phages of Lactobaciilus casei (PLC) isolated from plant drainage were classified and characterized. The results are as follows : 1. On the basis of host range pattern, phages could be divided into 2 groups (PLC-B and PLC-C). PLC-B group phages could be further divided into 5 sub-groups $(B_1, \;B_2, \;B_3, \;B_4, \;and\;B_5)$. Although PLC-C group phages had the same host range, they could be also divided into 2 sub-groups $(C_1\;and\;C_2)$ by morphlogical type. 2. It was $B_3$ group phages that represented a major proportion (44.4%) of phages tested. However, $B_1$ group phages were shown to have the widest host range. 3. Electron micrographs revealed that the phages fell into three different morphological types. $(B_1, \;B_2, \;and\;B_3)$ group phages hd a hexagonal head (52nm in diameter) and a sheathless noncontractile (245 nm in length). $B_4\;and\;C_2$ group phages had a hexagonal head (56 nm) and a short flexible tail (169nm) having no sheath. $B_5\;and\;C_1$ group phages were shown to have a hexagonal head (81 nm) and a contractile tail (140 nm) having a sheath, a base plate and tail fibers. 4. The inactivation of the phages by antisera indicated that serological relationships correlated completely with morphological types. 5. $B_1, \;C_1\;and\;C_2$ group phages produced a large (1, 2 mm in diameter) plaque with a clear ring. The morphology of plaques of $B_3\;and\;B_5$ group phages was the same as those produced by the above, but the average plaque sizes for $B_3\;and\;B_5$ were 0.8 mm abd 0.5 mm, respectively. $B_2\;and\;B_4$ group phages produced a small (0.5 mm) turbid plaque with an irregular edge. 6. The latent period and the average burst size of $B_1\;and\;B_3$ group phages were 90 min and 100, respectively. These phages reuqired calcium ions for their miltiplication. 7. $B_3$ group phages could not be absrobed to R-variant $KC_1$. 8. The order of resistance of phages to heat was $B_2\;>\;B_1, B_4\;and\;B_5\;>\;B_3\;and\;C_2, \;B_5$ group phages were more stable than $B_3$ in various pH values. $C_2$ group phages were more sensitive to UV irradiation than $B_1\;and\;B_3$ group phages. 9. Strains YIT9018 and IAM 1043 were induced by mitomycin C treatment. Phage particles detected in the lysates had a hexagonal head (38 and 49 nm, respectively), but no tail. Any sensitive indicator strain could not be isolated in spite of repaeated trials.
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김영창,정해일 인제대학교 1992 仁濟醫學 Vol.13 No.2
동시에 양측성으로 발생한 불안정 슬관절 환자중 인대 손상의 연관성을 조사하고 일측성과 비교하여 양측성의 경우 치료에 있어서의 상이점을 고찰해 보았다. The causes of knee instability were mainly traffic accidents and industrial accidents. The results of treatment, conservative or surgiral, were good. The purpose of this study were to establish the relationship of injured ligaments and to clarify any differences in treatment between bilateral knee instability and unilateral one. The conclusions were as follows, 1.There was relationship between injury of medial collateral ligament and contralateral lateral collateral ligament injury, and in all cases whose anterior and posterior ligament rupture in one knee joint, contralateral anterior eructate ligaments were ruptured. 2.The was no regularity in reupture site, femoral, tivial attachment or midportion. 3.The period of rehabilitation in bilateral knee instability was longer than unilateral knee instability.
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Penicillin G acylase 유전자의 구조와 발현기작에 관한 연구 I
김영창,구용범,오상진,강현삼 한국미생물학회 1983 미생물학회지 Vol.21 No.2
The penicillin G acylase(pga) gene was cloned in the vector plasmid pKM $300(Ar^r,\;Tc^r,\;6.33kb)$ for the study of the structure and expression of the pga gene. This recombinant plasmid pPAKS-1 DNA(24.5 Kb) was cleaved into 2 fragments by restriction enzyme Eco R1.1fragment by BamH1, 4fragments by Hind III, and 2 fragments by Pst I. The pga gene was located on the Eco R1.Hind III-C fragement of pPAKS-1. The recombinant plasmids pPAKS-1 and pPAKS-2, in which the Hind III-B and Hind III-D fragments pPAKS-1 are deleted, are characterized. The results are summarized as follows : 1. Doubling times of bacterial strain bearing pPAKS-1 and pPAKS-2 are 90 and 60 minutes, respectively. 2. pPAKS-1 and pPAKS-2 are present at about 16-32 and 70 copies per cell, respectively, are 0.66 and 5.5 units, respectively, which represent 2-fold and 20-fold higher enzyme 4. pPAKS-1 is very unstable, but pPAKS-2 is stable.
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Pseudomonas sp. strain DJ77 균주에서 extradiol dioxygenase 를 암호화하고 있는 phnE 유전자의 염기배열
김영창,신명수,윤길상,박영순,김욱현 한국미생물학회 1992 미생물학회지 Vol.30 No.1
Pseudomonas sp. DJ77로부터 extradiol dioxygenase 유전자(phnE)를 클로닝하고 염기배열을 결정하였다. 921 bp의 open reading frame (ORF) 이 존재하였고 개시코돈 앞에서 Shine-Dalgarno sequence를 발견하였다. phnE 유전자에서 만들어지는 PhnE 단백질은 분자량이 34,449 Da 인데 SDS-polycrylamide gel 전기영동에 의해 측정된 분자량과 일치하였다. PhnE는 NahH, XylE, DmpB 등과 아미노산 배열의 상동성의 약 50% 였다. DJ77에는 bphC와 같은 3형의 extradiol dioxygenase 유전자는 발견할 수 없었다. DJ77 과 JM101(pPE17)은 catechol, 3-methylcatechol, 4-methylcatechol, 2, 3-dihydroxybiphenyl 등의 기질을 meta-cleavage 하여 노란색 화합물을 생성할 수 있었다. Nucleotide Sequence of phnE Gene Encoding Extradiol Dioxygenase fromPseudomonas sp. Strain DJ77Kim, Young-Chang'.", Myeong-Su Shin1, Kil-Sang Younl, Young-Soon Park1, andUg-Hyeon Kim'.' (Department of Microbiology, C'hungbuk National University.Cheongju 360-763, KOREA. and 'Research Center for Molecular Microbiology,Seoul National University)nal University)
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