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[PD-0017] Mutation of GmIPK1 Gene using CRISPR/Cas9 Reduced Phytic Acid Content in Soybean Seeds
Hye Jeong Kim(Hye Jeong Kim),Ji Hyeon Song(Ji Hyeon Song),Gilok Shin(Gilok Shin),Hyeonjin Song(Hyeonjin Song),Ji Eun Lee(Ji Eun Lee),Cha Young Kim(Cha Young Kim),Young-Soo Chung(Young-Soo Chung) 한국육종학회 2022 한국육종학회 공동학술발표집 Vol.2022 No.-
세대단축시스템을 이용한 국내 밀 품종의 등숙기 강우에 의한 품질변이 평가
박현진(Hyeonjin Park),차진경(Jin-Kyung Cha),이소명(So-Myeong Lee),권영호(Youngho Kwon),최지수(Jisu Choi),오기원(Ki-Won Oh),이종희(Jong-Hee Lee) 한국작물학회 2023 한국작물학회지 Vol.68 No.3
밀 등숙기 강우의 영향을 분석하기 위해 백립계인 조경과 적립계인 황금알을 이용하여 출수기 이후 시기별 인공강우처리에 의한 품질을 분석하였다. 그 결과, 종자 단면은출수기 이후 35일부터 분상질화되기 시작하였고, 출수기이후 40일부터 ΔL값과 ΔE* ab 값이 증가하기 시작하였다. 출수기 이후 55일에 조경은 종자 단면 전체가 분상질화 되었지만, 황금알은 초자질 비율이 높게 유지되어 품질변이에 강할 것으로 예상되었다. 주사전자현미경을 이용한 종자 내 전분입자 촬영 결과, 조경은 출수기 이후 40일부터, 황금알은 50일부터 A-, B-granule이 분해되는 것을 관찰할수 있었다. 종자 품질분석 결과, 단백질 함량은 처리시기별 경향성을 나타내지 않았으나, 회분은 출수기 이후 45일처리부터 유의하게 증가하였고 침전가는 출수기 이후 35일부터 감소하였다. 따라서 밀 등숙 전반기에는 강우의 영향을 적게 받지만, 출수기 이후 40~45일부터는 강우에 의한 품질변이에 취약할 것으로 예상된다. 또한 적립계 밀 품종은 백립계에 비하여 수발아에 강한 것으로 알려져 있으며, 본 연구에서도 황금알이 조경에 비해 등숙 후반기 강우에도 품질이 높게 유지되는 것으로 나타났다. 따라서 밀 종피색와 품질변이 간 관계에 대한 추가적인 검토가 필요할 것으로 판단된다. Wheat (Triticum aestivum L.) is an important crop in Korea, with a per capita consumption of 31.6 kg in 2019. In the southern region, wheat is grown after paddy rice, and it is harvested during the rainy season in mid-June. This timing, in combination with high humidity and untimely rainfall, activates the enzyme alpha-amylase, which breaks down starch in the wheat grains. As a result, sprouted grains have lower quality and value for flour. However, seeds that absorb water before sprouting are expected to maintain better quality. The aim of the study was to identify the critical period during wheat maturation when rainfall has the greatest impact on grain quality, to prevent price declines due to quality deterioration. Two wheat cultivars, Jokyoung and Hwanggeumal, were grown in a speed breeding room, and artificial rainfall was applied at different times after heading (30, 35, 40, 45, 50, and 55 days). The proportion of vitreous grains decreased from 40 to 55 days after heading (DAH). Both cultivars had chalky grain sections from 35 DAH, with Hwanggeumal having a higher proportion of vitreous grains. Starch degradation was observed using FE-SEM (Field Emission Scanning Electron Microscope) at 40 DAH for Jokyoung and 50 DAH for Hwanggeumal. Color measurements indicated increased L and E values from 40 DAH, with rain treatment at 55 DAH leading to a significant increase in L values for both cultivars. Ash content increased at 45 DAH, whereas SDSS decreased at 35 DAH. Overall, grain quality from 40 DAH until harvest was found to be affected to the greatest extent by direct exposure of the spikes to moisture. Red wheat showed better quality than white wheat. These findings have implications for the cultivation of high-quality wheat and can guide future research efforts in this area.
Effects of Sangju Honey on Oral Squamous Carcinoma Cells
Nangwon Yee,Hyeonjin Kim,Eungyung Kim,Yong Ho Cha,Lei Ma,Na Eun Cho,Dongwook Kim,Chae Yeon Kim,Sung-Hyun Kim,Zaeyoung Ryoo,이준구,김명옥 대한암예방학회 2022 Journal of cancer prevention Vol.27 No.4
Since ancient times, honey has been used in traditional medicine owing to its pharmacological effects. It possesses anticancer properties. However, the therapeutic implications of Sangju honey in cancer remains unknown. Therefore, we aimed to demonstrate the potential anticancer effects of Sangju honey on human oral squamous cell carcinoma (OSCC), particularly focusing on epithelial–mesenchymal transition (EMT) and apoptotic and mitogen-activated protein kinase (MAPK) signaling pathways. Ca9- 22 and YD-10B human OSCC cells were treated with 0.25% or 0.5% Sangju honey, and the cell viability was examined using the Cell Counting Kit-8 assay. Cell morphology studies were conducted to observe morphological changes, and the wound-healing assay was performed to evaluate the proliferation of honey-treated OSCC cells. Western blot analysis was conducted to investigate protein expression related to EMT and apoptotic and MAPK signaling pathways. Sangju honey reduced cell viability, induced morphological changes, and significantly suppressed the proliferation and migration of Ca9-22 and YD-10B cells. The expression of E-cadherin and N-cadherin was increased and decreased, respectively, in both OSCC cell lines. Moreover, Sangju honey stimulated apoptosis by increasing the expression of p21, p53, cleaved caspase 3, and caspase 9. Furthermore, it downregulated the expression of phospho (p)-extracellular signal-regulated kinases 1 and 2, p-c-Jun amino-terminal kinase, and p-p38 in Ca9-22 and YD-10B cells. Sangju honey inhibits Ca9-22 and YD-10B cell proliferation by regulating EMT, inducing apoptosis, and suppressing the MAPK signaling pathway. Thus, it is a potential anticancer agent for human OSCC.
장동균 ( Donggune Chang ),최현진 ( Hyeonjin Choi ),김희선 ( Heesun Kim ),차승현 ( Seunghyun Cha ),이은환 ( Eunwhan Lee ) 경희대학교 경영연구원 2015 의료경영학연구 Vol.9 No.3
This study aims to estimate the economic cost due to at opic dermatitis in Korea. The national patient sample data (NPS) of Health Insurance Review and Assessment Service (HIRA) was used to measure the cost of illness due to atopic dermatitis, and this study included both direct and indirect costs. Direct cost is composed of treatment, medication, transportation, and caregiver’s time cost. Indirect costs associated with the loss of productivity in atopic dermatitis patients, such as absence of work, income loss due to premature death in the future. The results show that the socio-economic burden of atopic dermatitis is about 90 billion won in Korea. Furthermore, this study identified that children and adolescents are more vulnerable. The results of this study can be valuable for determining the priority of health policy and intervention strategies to reduce the burden of disease due to atopic dermatitis.