The Associated Factors of Peripheral Arterial Occlusive Disease in Patients after Kidney Transplantation

Hsiu-Ying Lin,Hsueh-Erh Liu 한국간호과학회 2015 한국간호과학회 학술대회 Vol.2015 No.10

Virtual Screening and Testing of GSK-3 Inhibitors Using Human SH-SY5Y Cells Expressing Tau Folding Reporter and Mouse Hippocampal Primary Culture under Tau Cytotoxicity

Lin Chih-Hsin,Hsieh Yu-Shao,Sun Ying-Chieh,Huang Wun-Han,Chen Shu-Ling,Weng Zheng-Kui,Lin Te-Hsien,Wu Yih-Ru,Chang Kuo-Hsuan,Huang Hei-Jen,Lee Guan-Chiun,Hsieh-Li Hsiu Mei,Lee-Chen Guey-Jen 한국응용약물학회 2023 Biomolecules & Therapeutics(구 응용약물학회지) Vol.31 No.1

Glycogen synthase kinase-3β (GSK-3β) is an important serine/threonine kinase that implicates in multiple cellular processes and links with the neurodegenerative diseases including Alzheimer’s disease (AD). In this study, structure-based virtual screening was performed to search database for compounds targeting GSK-3β from Enamine’s screening collection. Of the top-ranked compounds, 7 primary hits underwent a luminescent kinase assay and a cell assay using human neuroblastoma SH-SY5Y cells expressing Tau repeat domain (TauRD) with pro-aggregant mutation ΔK280. In the kinase assay for these 7 compounds, residual GSK-3β activities ranged from 36.1% to 90.0% were detected at the IC50 of SB-216763. In the cell assay, only compounds VB-030 and VB-037 reduced Tau aggregation in SH-SY5Y cells expressing ΔK280 TauRD-DsRed folding reporter. In SH-SY5Y cells expressing ΔK280 TauRD, neither VB-030 nor VB-037 increased expression of GSK-3α Ser21 or GSK-3β Ser9. Among extracellular signal-regulated kinase (ERK), AKT serine/threonine kinase 1 (AKT), mitogen-activated protein kinase 14 (P38) and mitogenactivated protein kinase 8 (JNK) which modulate Tau phosphorylation, VB-037 attenuated active phosphorylation of P38 Thr180/ Tyr182, whereas VB-030 had no effect on the phosphorylation status of ERK, AKT, P38 or JNK. However, both VB-030 and VB-037 reduced endogenous Tau phosphorylation at Ser202, Thr231, Ser396 and Ser404 in neuronally differentiated SH-SY5Y expressing ΔK280 TauRD. In addition, VB-030 and VB-037 further improved neuronal survival and/or neurite length and branch in mouse hippocampal primary culture under Tau cytotoxicity. Overall, through inhibiting GSK-3β kinase activity and/or p-P38 (Thr180/Tyr182), both compounds may serve as promising candidates to reduce Tau aggregation/cytotoxicity for AD treatment.

The Short-Term Fear Effects for Taiwan's Equity Market from Bad News Concerning Sino-U.S. Trade Friction

YANG, Shu Ya,LIN, Hsiu Hsu,LIU, Ying Sing Korea Distribution Science Association 2021 The Journal of Asian Finance, Economics and Busine Vol.8 No.3

Mainland China area has been a long-term, major trade rival and partner of Taiwan, accounting for more than 40% of Taiwan's total annual trade exports, and so Sino-US trade friction is expected to have a significant impact on Taiwan's economy in the future. This study focuses on major bad news of Sino-US trade frictions and how it generates short-term shocks for Taiwan's equity market and fear sentiment. It further explores the mutual interpretation relationship between price changes such as VIX, Taiwan's stock market index, and the VIX ETF to identify which factors have information leadership as leading indicators. The study period covers 750 trading days from 2017/1/3 to 2020/1/31. This study finds that, when a policy news is announced, the stock market index falls significantly, the change in the trading price (net value) of the VIX ETF rises significantly, and the overprice rate significantly drops, but VIX does not, showing that fear sentiment exists in the Taiwan's market. The net value of the VIX ETF shows an information advantage as a leading indicator. This study suggests that, when the world's two largest economies clash over trade, the impact on Taiwan's equity market is inevitable, and that short-term fear effects will arise.

Measuring the Development of Knowledge-Based Economy in Taiwan

Yang, Chia Yen,Lin, Hsiu Ying 이화여자대학교 국제통상협력연구소 2001 Asian International Studies Review Vol.4 No.1/2

The progress of information and communication technology (ICT) has been regarded as one crucial factor bringing about the phenomenon of knowledge-based economy, while other factors such as human resource development, and related institutional settings have also played important roles behind the scene. Our measurement reflects Taiwan's potential knowledge-based economy (KBE) developments, which nevertheless indicates considerable room for improvement. Except for ICT investment, the other knowledge investment indicators supported the perspective of Taiwan's KBE development. However, the knowledge diffusion is perhaps the weakest part of Taiwan's KBE development; Taiwan needs much improvement in order to stay close to the developed countries, finally, as for the knowledge output and application, Taiwan's outstanding performance in research publication and patents is consistent with that in research and development (R&D) of human resources, but the obviously weak performance of knowledge-intensive service sector might have result from Taiwan's relative disadvantages indicated by the measurement of the knowledge diffusion. Given the above status of Taiwan's KBE development, Taiwan will inevitably encounter the following two major challenges; one is related to the difficulty in upgrading Taiwan's science and technology (S&T), and the other is related to the noted issues of digital divide and economic stability.

The Global Competitiveness of Taiwan`s Industrial Technology

Wu, Rong I,Lin, Hsiu Ying 이화여자대학교 국제통상협력연구소 2001 Asian International Studies Review Vol.4 No.1/2

For more than a decade, Taiwan has been working actively on programs for the localization of technology. Taiwan is not only ranked the world's third largest supplier of information and technology (IT) products, but it is also ranked fourth in technology and third in innovation according to the 2001 World Economy Forum (WEF) " Global Competitiveness Report." Overall, the competitiveness of Taiwan's technology has been very impressive. Taiwan is not a member of the organization for Economic Cooperation and Development (OECD). With report to Taiwan's technological innovation capability and national competitiveness of each individual industry, the indices to make direct comparison with OECD are still under construction. In consideration of data comparability and availability, this research aims to establish a set of indicators that can be used to compare the competitiveness of Taiwan's industrial technology with other countries, so as to understand the technological performance of Taiwan's industrial technology and its international status. These assessment indices include indicators representing current competitiveness and output (such as patents, licensing feeds and royalties, added values, and export indicators), as well as indicators to represent the input competitiveness index (research and development (R&D) investment and researcher). The method of analysis includes comparing of individual indicators, as well as using patents and market share for cross-analysis, in order to study changes in Taiwan and its competitors. The results of comparison and analysis show that Taiwan experienced a slow downturn on inputs in 2000. In both hi-tech industry as a whole the R&D expenditure growth rate and R&D density were lower than in 1999. Because R &D and innovation are significantly related to Taiwan's future competitiveness, causes for the slack in R&D investment growth need to be confirmed in order for the government to make the industrial policy. AS to the performance on output, patent performance has been outstanding despite the number of granted patents, patent strength, or impact. It reflects the fact that Taiwan has become an important player on the international innovation stage. however, Taiwan's industrial structure is highly concentrated in the information and communication technology (ICT) sector, and the weak demand in the ICT market following the September 11th terrorist attacks in the United States had a serious impact on the export competitiveness of Taiwan's hi-tech industries in 2001. In the future, enhancing the density of industrial technology R&D investmel future risks will be Taiwan's next challenge.

Monitoring of genetically close Tsaiya duck populations using novel microsatellite markers with high polymorphism

Lai, Fang-Yu,Chang, Yi-Ying,Chen, Yi-Chen,Lin, En-Chung,Liu, Hsiu-Chou,Huang, Jeng-Fang,Ding, Shih-Torng,Wang, Pei-Hwa Asian Australasian Association of Animal Productio 2020 Animal Bioscience Vol.33 No.6

Objective: A set of microsatellite markers with high polymorphism from Tsaiya duck were used for the genetic monitoring and genetic structure analysis of Brown and White Tsaiya duck populations in Taiwan. Methods: The synthetic short tandem repeated probes were used to isolate new microsatellite markers from the genomic DNA of Tsaiya ducks. Eight populations, a total of 566 samples, sourced from Ilan Branch, Livestock Research Institute were genotyped through novel and known markers. The population genetic variables were calculated using optional programs in order to describe and monitor the genetic variability and the genetic structures of these Tsaiya duck populations. Results: In total 24 primer pairs, including 17 novel microsatellite loci from this study and seven previously known loci, were constructed for the detection of genetic variations in duck populations. The average values for the allele number, the effective number of alleles, the observed heterozygosity, the expected heterozygosity, and the polymorphism information content were 11.29, 5.370, 0.591, 0.746, and 0.708, respectively. The results of analysis of molecular variance and principal component analysis indicated a contracting Brown Tsaiya duck cluster and a spreading White Tsaiya duck cluster. The Brown Tsaiya ducks and the White Tsaiya ducks with Pekin ducks were just split to six clusters and three clusters when K was set equal to 6 and 3 in the Bayesian cluster analysis. The individual phylogenetic tree revealed eight taxa, and each individual was assigned to its own population. Conclusion: According to our study, the 24 novel microsatellite markers exhibited a high capacity to analyze relationships of inter- and intra-population in those populations with a relatively limited degree of genetic diversity. We suggest that duck farms in Taiwan could use the new (novel) microsatellite set to monitor the genetic characteristics and structures of their Tsaiya duck populations at various intervals in order to ensure quality breeding and conservation strategies.

The Presence of Borrelia valaisiana-Related Genospecies in Ticks and a Rodent in Taiwan

Chun-Man Huang,Hsi-Chieh Wang,Ying-Chun Lin,Shih-Hui Chiu,Ying-Shun Kao,Pei-Lung Lee,Hsiu-I Wang,Ruei-Chen Hung,Huang-I Chan,Ho-Sheng Wu,Chuen-Sheue Chiang,Jung-Jung Mu 한국미생물학회 2010 The journal of microbiology Vol.48 No.6

A field survey was conducted to investigate the presence of Borrelia burgdorferi sensu lato (s.l.) in six counties of Taiwan. Spirochetes were successfully isolated from one rodent ear sample out of 485 rodent ears and 53live, fed tick (Ixodes granulatus) samples. The spirochetes were confirmed to be B. burgdorferi s.l. by real-time PCR. In addition, 23 of 113 tick samples were tested positive for Borrelia DNA according to real-time PCR. The Borrelia isolate from the rodent and the 23 Borrelia DNA samples from the ticks were identified as B. valaisiana-related genospecies by phylogenetic analysis based on flagellin gene sequences. These findings suggest that the Borrelia valaisiana-related strains are maintained in a zoonotic cycle between tick vectors and reservoir hosts in Taiwan.

Mesenchymal Stem Cell Secreted-Extracellular Vesicles are Involved in Chondrocyte Production and Reduce Adipogenesis during Stem Cell Differentiation

Tsai Yu-Chen,Cheng Tai-Shan,Liao Hsiu-Jung,Chuang Ming-Hsi,Chen Hui-Ting,Chen Chun-Hung,Zhang Kai-Ling,Chang Chih-Hung,Lin Po-Cheng,Huang Chi-Ying F. 한국조직공학과 재생의학회 2022 조직공학과 재생의학 Vol.19 No.6

BACKGROUND: Extracellular vesicles (EVs) are derived from internal cellular compartments, and have potential as a diagnostic and therapeutic tool in degenerative disease associated with aging. Mesenchymal stem cells (MSCs) have become a promising tool for functional EVs production. This study investigated the efficacy of EVs and its effect on differentiation capacity. METHODS: The characteristics of MSCs were evaluated by flow cytometry and stem cell differentiation analysis, and a production mode of functional EVs was scaled from MSCs. The concentration and size of EVs were quantitated by Nanoparticle Tracking Analysis (NTA). Western blot analysis was used to assess the protein expression of exosomespecific markers. The effects of MSC-derived EVs were assessed by chondrogenic and adipogenic differentiation analyses and histological observation. RESULTS: The range of the particle size of adipose-derived stem cells (ADSCs)- and Wharton’s jelly -MSCs-derived EVs were from 130 to 150 nm as measured by NTA, which showed positive expression of exosomal markers. The chondrogenic induction ability was weakened in the absence of EVs in vitro. Interestingly, after EV administration, type II collagen, a major component in the cartilage extracellular matrix, was upregulated compared to the EV-free condition. Moreover, EVs decreased the lipid accumulation rate during adipogenic induction. CONCLUSION: The results indicated that the production model could facilitate production of effective EVs and further demonstrated the role of MSC-derived EVs in cell differentiation. MSC-derived EVs could be successfully used in cell-free therapy to guide chondrogenic differentiation of ADSC for future clinical applications in cartilage regeneration.

The Applications of Smart Wearable Devices for Detecting Balance Function in Individuals at High Risk of Falls

Tsung-Hsun Hsieh,Chih-Wei Peng,Kai-Yun Chen,Ying-Zu Huang,Yi-Huang Lin,Wei-Zong Zhong,Chun-Yu Cheng,Ya-Ju Chang,Chih-Hsiu Cheng,Yu-Fen Chuang 한국재활복지공학회 2017 한국재활복지공학회 학술대회논문집 Vol.2017 No.11

Combined Assessment of Serum Alpha-Synuclein and Rab35 is a Better Biomarker for Parkinson’s Disease

Hung-Li Wang,Chin-Song Lu,Tu-Hsueh Yeh,Yu-Ming Shen,Yi-Hsin Weng,Ying-Zu Huang,Rou-Shayn Chen,Yu-Chuan Liu,Yi-Chuan Cheng,Hsiu-Chen Chang,Ying-Ling Chen,Yu-Jie Chen,Yan-Wei Lin,Chia Chen Hsu,Huang-Li 대한신경과학회 2019 Journal of Clinical Neurology Vol.15 No.4

Background and Purpose It is essential to develop a reliable predictive serum biomarker for Parkinson’s disease (PD). Te accumulation of alpha-synuclein (αSyn) and up-regulated expression of Rab35 participate in the etiology of PD. Te purpose of this investigation was to determine whether the combined assessment of serum αSyn and Rab35 is a useful predictive biomarker for PD. Methods Serum levels of αSyn or Rab35 were determined in serum samples from 59 sporadic PD patients, 19 progressive supranuclear palsy (PSP) patients, 20 multiple system atrophy (MSA) patients, and 60 normal controls (NC). Receiver operating characteristics (ROC) curves were calculated to determine the diagnostic accuracy of αSyn or/and Rab35 in discriminating PD patients from NC or atypical parkinsonian patients. Results The levels of αSyn and Rab35 were increased in PD patients. The serum level of Rab35 was positively correlated with that of αSyn in PD patients. Compared to analyzing αSyn or Rab35 alone, the combined analysis of αSyn and Rab35 produced a larger area under the ROC curve and performed better in discriminating PD patients from NC, MSA patients, or PSP patients. When age was dichotomized at 55, 60, 65, or 70 years, the combined assessment of αSyn and Rab35 for classifying PD was better in the group below the cutof age than in the group above the cutof age. Conclusions Combined assessment of serum αSyn and Rab35 is a better biomarker for discriminating PD patients from NC or atypical parkinsonian patients, and is a useful predictive biomarker for younger sporadic PD patients.