인동덩굴로부터 분리된 Cynaroside이 Doxorubicin으로 유도된 인간 근위세뇨관 HK-2 세포의 괴사에 미치는 저해 효과

노종현,정호경,이무진,장지훈,심미옥,정자균,정다은,안병관,조현우,Nho, Jong Hyun,Jung, Ho Kyung,Lee, Mu Jin,Jang, Ji Hun,Sim, Mi Ok,Jung, Ja Kyun,Jung, Da Eun,An, Byeong Kwan,Cho, Hyun Woo 한국약용작물학회 2017 한국약용작물학회지 Vol.25 No.5

Background: Cynaroside is a flavone, a flavonoid-like compound, known by different names (luteoloside and cinaroside). It is commonly found in Lonicera japonica Thunb., Chrysanthemum moriflium, and Angelica keiskei. The process of cell death has been classified as necrosis and apoptosis. Necrosis refers to unregulated cell death induced by a chemotherapeutic agent. Doxorubicin is an anthracycline anti-cancer drug used to treat acute leukemia, cancer, and lymphoma. However, it induces nephrotoxicity including tubular damage. Therefore, we investigated the protective effect of cynaroside against doxorubicin-induced necrosis in HK-2 cells. Methods and Results: To confirm the beneficial effect of cynaroside on doxorubicin-induced necrosis, HK-2 cells, a human proximal tubule epithelial cell line were treated with $10{\mu}M$ doxorubicin and $80{\mu}M$ cynaroside. Doxorubicin treatment resulted in increased DNA fragmentation, caspase-3 activity and mitochondria hyperactivation during cell necrosis. However, pretreatment with $80{\mu}M$ cynaroside attenuated DNA fragmentation, caspase-3 activity and mitochondria hyperactivation induced by $10{\mu}M$ doxorubicin in HK-2 cells. Conclusions: These results indicated that pretreatment with cynaroside ameliorated doxorubicin-induced necrosis in HK-2 cells. Therefore, cynaroside be used as a therapeutic agent for improving doxorubicin-induced nephrotoxicity. However, further studies are required to evaluated the toxicity of cynaroside treatment in animals and to determine its protective effect against doxorubicin-induced nephrotoxicity in an animal model.

UHF RFID 태그 칩용 저전력 EEPROM설계

이원재,이재형,박경환,이정환,임규호,강형근,고봉진,박무훈,하판봉,김영희,Yi, Won-Jae,Lee, Jae-Hyung,Park, Kyung-Hwan,Lee, Jung-Hwan,Lim, Gyu-Ho,Kang, Hyung-Geun,Ko, Bong-Jin,Park, Mu-Hun,Ha, Pan-Bong,Kim, Young-Hee 한국정보통신학회 2006 한국정보통신학회논문지 Vol.10 No.3

본 논문에서 는 플래쉬 셀을 사용하여 수동형 UHF RFID 태그 칩에 사용되는 저전력 1Kb 동기식 EEPROM을 설계하였다. 저전력 EEPROM을 구현하기 위한 방법으로 다음과 같은 4가지 방법을 제안하였다. 첫째, VDD(=1.5V)와 VDDP(=2.5V)의 이중 전원 공급전압 방식을 사용하였고, 둘째, 동기식 회로 설계에서 클럭(clock) 신호가 계속 클럭킹(clocking)으로 인한 스위칭 전류(switching current)가 흐르는 것을 막기 위해 CKE(Clock Enable) 신호를 사용하였다. 셋째, 읽기 사이클에서 전류 센싱(current sensing) 방식 대신 저전력 소모를 갖는 clocked inverter를 사용한 센싱 방식을 사용하였으며, 넷째, 쓰기 모드시 Voltage-up 변환기(converter) 회로를 사용하여 기준전압 발생기(Reference Voltage Generator)에는 저전압인 VDD를 사용할 수 있도록 하여 전력 소모를 줄일 수가 있었다. $0.25{\mu}m$ EEPROM 공정을 이용하여 칩을 제작하였으며, 1Kb EEPROM을 설계한 결과 읽기 모드와 쓰기 모드 시에 소모되는 전력은 각각 $4.25{\mu}W$와 $25{\mu}W$이고, 레이아웃 면적(layout area)은 $646.3\times657.68{\mu}m^2$이다. In this paper, a low-power 1Kb synchronous EEPROM is designed with flash cells for passive UHF RFID tag chips. To make a low-power EEPROM, four techniques are newly proposed. Firstly, dual power supply voltages VDD(1.5V) and VDDP(2.5V), are used. Secondly, CKE signal is used to remove switching current due to clocking of synchronous circuits. Thirdly, a low-speed but low-power sensing scheme using clocked inverters is used instead of the conventional current sensing method. Lastly, the low-voltage, VDD for the reference voltage generator is supplied by using the Voltage-up converter in write cycle. An EEPROM is fabricated with the $0.25{\mu}m$ EEPROM process. Simulation results show that power dissipations are $4.25{\mu}W$ in the read cycle and $25{\mu}W$ in the write cycle, respectively. The layout area is $646.3\times657.68{\mu}m^2$.

Anti-inflammatory Effect of LFR on LPS-stimulated THP-1 Cells

정승기,Sul, Mu-Chang,Kim, Hong-Yeoul,Roh, Young-Lae,Jung, Hee-Jae,Jung, Sung-Ki 대한한방내과학회 2009 大韓韓方內科學會誌 Vol.30 No.2

Background and Objective: Luffae Fructus Retinervus (LFR) is used for investigating symptoms of inflammation. We have evaluated the anti-inflammatory effect of LFR by analyzing the expression of pro-inflammatory cytokines. Materials and Methods : We differentiated THP-l cells into macrophage-like cells by treatment with PMA. Inflammation was induced by treatment with LPS and PMA. We determined the safe concentration of LFR by using the MTS and MTT assays and using PD 98059 as a negative control for comparison of the anti-inflammatory effect of LFR. Results : The MTS and MTT analysis showed that the cell survival rate was >80% within the LFR concentration range of 10-100 ng/ml and began to decrease to >80% at 1 ${\mu}g/ml$. By RT-PCR analysis, the gene expression of TNF-${\alpha}$, IL-8, TGF-${\beta}$, IL-6, IL-${\beta}$1, and IL-10 levels were down-regulated when monocyte-derived macrophages were treated with concentrations of LFR between 10 ng/mL and 100 ng/mL. Conclusion : We conclude that LFR exerts an anti-inflammatory effect by inhibiting the expression of pro-inflammatory activity. The results suggest a promising way to treat general inflammatory diseases.

Control of IPMC-based Artificial Muscle for Myoelectric Hand Prosthesis

Lee Myoung-Joon,Jung Sung-Hee,Moon Inhyuk,Lee Sukmin,Mun Mu-Seong The Korean Society of Medical and Biological Engin 2005 의공학회지 Vol.26 No.5

This paper proposes an ionic polymer metal composite (IPMC) based artificial muscle to be applicable to the Myoelectric hand prosthesis. The IPMC consists of a thin polymer membrane with metal electrodes plated chemically on both faces, and it is widely applying to the artificial muscle because it is driven by relatively low input voltage. The control commands for the IPMC-based artificial muscle is given by electromyographic (EMG) signals obtained from human forearm. By an intended contraction of the human flexor carpi ulnaris and extensor carpi ulnaris muscles, we investigated the actuation behavior of the IPMC-based artificial muscle. To obtain higher actuation force of the IPMC, the single layered as thick as $800[{\mu}m]$ or multi-layered IPMC of which each layer can be as thick as $178[{\mu}m]$ are prepared. As a result, the bending force was up to the maximum 12[gf] from 1[gf] by actuating the single layered IPMC with $178[{\mu}m]$, but the bending displacement was reduced to 6[mm] from 30[mm]. The experimental results using an implemented IPMC control system show a possibility and a usability of the bio-mimetic artificial muscle.

Beneficial Effects of Cynaroside on Cisplatin-Induced Kidney Injury In Vitro and In Vivo

Nho, Jong-Hyun,Jung, Ho-Kyung,Lee, Mu-Jin,Jang, Ji-Hun,Sim, Mi-Ok,Jeong, Da-Eun,Cho, Hyun-Woo,Kim, Jong-Choon Korean Society of ToxicologyKorea Environmental Mu 2018 Toxicological Research Vol.34 No.2

Anti-cancer drugs such as cisplatin and doxorubicin are effectively used more than radiotherapy. Cisplatin is a chemotherapeutic drug, used for treatment of various forms of cancer. However, it has side effects such as ototoxicity and nephrotoxicity. Cisplatin-induced nephrotoxicity increases tubular damage and renal dysfunction. Consequently, we investigated the beneficial effect of cynaroside on cisplatin-induced kidney injury using HK-2 cell (human proximal tubule cell line) and an animal model. Results indicated that $10{\mu}M$ cynaroside diminished cisplatin-induced apoptosis, mitochondrial dysfunction and caspase-3 activation, cisplatin-induced upregulation of caspase-3/MST-1 pathway decreased by treatment of cynaroside in HK-2 cells. To confirm the effect of cynaroside on cisplatin-induced kidney injury in vivo, we used cisplatin exposure animal model (20 mg/kg, balb/c mice, i.p., once a day for 3 days). Renal dysfunction, tubular damage and neutrophilia induced by cisplatin injection were decreased by cynaroside (10 mg/kg, i.p., once a day for 3 days). Results indicated that cynaroside decreased cisplatin-induced kidney injury in vitro and in vivo, and it could be used for improving cisplatin-induced side effects. However, further experiments are required regarding toxicity by high dose cynaroside and caspase-3/MST-1-linked signal transduction in the animal model.

A New Histone Deacetylase Inhibitor, MHY219, Inhibits the Migration of Human Prostate Cancer Cells via HDAC1

De, Umasankar,Kundu, Soma,Patra, Nabanita,Ahn, Mee Young,Ahn, Ji Hae,Son, Ji Yeon,Yoon, Jung Hyun,Moon, Hyung Ryoung,Lee, Byung Mu,Kim, Hyung Sik The Korean Society of Applied Pharmacology 2015 Biomolecules & Therapeutics(구 응용약물학회지) Vol.23 No.5

Histone deacetylase (HDAC) inhibitors are considered novel agents for cancer chemotherapy. We previously investigated MHY219, a new HDAC inhibitor, and its potent anticancer activity in human prostate cancer cells. In the present study, we evaluated MHY219 molecular mechanisms involved in the regulation of prostate cancer cell migration. Similar to suberanilohydroxamic acid (SAHA), MHY219 inhibited HDAC1 enzyme activity in a dose-dependent manner. MHY219 cytotoxicity was higher in LNCaP ($IC_{50}=0.67{\mu}M$) than in DU145 cells ($IC_{50}=1.10{\mu}M$) and PC3 cells ($IC_{50}=5.60{\mu}M$) after 48 h of treatment. MHY219 significantly inhibited the HDAC1 protein levels in LNCaP and DU145 cells at high concentrations. However, inhibitory effects of MHY219 on HDAC proteins levels varied based on the cell type. MHY219 significantly inhibited LNCaP and DU145 cells migration by down-regulation of matrix metalloprotease-1 (MMP-1) and MMP-2 and induction of tissue inhibitor of metalloproteinases-1 (TIMP-1). These results suggest that MHY219 may potentially be used as an anticancer agent to block cancer cell migration through the repression of MMP-1 and MMP-2, which is related to the reduction of HDAC1.

水分活性을 달리한 白米變溫貯藏中 포도당의 변화

정연화,전순실,김무남 新羅大學校 1992 論文集 Vol.34 No.1

A kinetical approach for the change of glucose in rice was studied. The rice was controlled at ??0.44, 0.55, 0.65, and 0.75 using saturated salt solutions and then stored at 30, 40, 50℃ and 30/50℃ alternately with 7days interval. The amount of glucose in the rice was increased by zero-order reaction during the earlier stage of storage and then decreased by first-order reaction. The reaction rates revealed a tend to increase with increasing water activities . The activation energies at ?? 0.44-0.75 were ranged 10.26-16.48Kcal/mole with increase in and 3.66-5.06Kcal/mole with decrease in the amount of glucose ?? values were ranged 1.70-2.34 with increase of glucose and 1.21-1.30 with decrease. The shelf-lives assessed at 25℃ from the accelerated shelf-life tests ranged from 112 days at ?? 0.75 to 163 days at ?? 0.44.

Cyclobutylsilane의 구조적 안정도

李武相,鄭英美 경북대학교 과학교육연구소 1990 科學敎育硏究誌 Vol.14 No.-

The molecular structure of cyclobutylsilane, c-C_4H_7SiH_3, has been investigated by molecular mechanics Ⅱ(MM2). Two single conformers(equatorial and axial) were considered. The resulting structural parameters of the equatorial conformer are as follow:Si-C2=1.8649 Å, Si-H7=1.4848 Å, <C2SiH6-110.375˚, <SiC2C3=115.713˚, dihedral angles W_1(SiC2C3C4)=138.122˚ and W_2(SiC2C3H10)=25.019˚. The final steric energies of equatorial and axial conformer was 28.35kcal/mol and 28.84kcal/mol respectively. These results were in excellent agreement with Raman spectra in the gas phase, and gas phase electron diffraction data.

酸化鐵의 缺乏構造에 關한 硏究

片茂實,姜參龍,徐廷善 명지대학교 1972 明大論文集 Vol.5 No.-

The defect form of the iron oxide system is studied by analyzing the gravimetric analysis of a sample measured by using a quartz microbalance(in a temperature range from O℃ to 1,200℃ under oxygen Pressures from 10^2mmHg to 10^-4 mmHg The Y Values of the formula FeO_1+y, are corretly obtained by this means for various condition of temp erature and pressure The plots of log Y v, s log P0_2 (or log Y=1/nlog Po_2) show linearity and calculates from the slope of the plot is about 1/10 at 1,000℃ The most of physical properties of iron oxide system such as electrical conductivity oxidation mechanism and catalytic effect is also discussed with respect to the defect form or the Y values.

생약 배합에 따른 탈모 방지 및 발모 촉진에 관한 연구

김무영,곽제훈,박정미,이진태,안봉전 경산대학교 생명자원개발연구소 2002 생명자원과 산업 Vol.6 No.-

본 연구에서 생약 추출물을 이용한 탈모 방지 및 발모 촉진에 대한 효과를 검증하고자 Norwood's V형 1명, Ⅵ형 1명, Ⅶ형 2명의 성인 남자 4명과 Ludwig's Ⅱ형의 여자 2명 모두 6명을 대상으로 8개월간 생약 도포와 복용을 동시에 실시하여 두피 변화와 발모 효과에 관찰하였다. 생약 추출물을 도포하고 복용한 이후, 탈모 수가 점차 줄어들고 있어 탈모 방지에 효과가 있는 것으로 나타났으며, 지정한 부위의 모발 생성을 찾아 볼 수 있었다. 도포 및 복용 실험 전 피검자들의 1㎠ 당 평균 모발수는 평균 150개의 모발이 생성되고 있는 정상인의 1/3 수준에도 이르지 못하는 약 44개였으나, 생약 추출물의 도포와 복용 실험에 들어간 지 3개월 후에는 두피 1㎠ 당 평균 약 57개로, 6개월 후에는 평균 약 88개로 각각 증가하였다. 도포 및 복용 후 발모로 인해 대인관계에 자신감을 느낄 수 있었으며, 미용에 관심을 더 갖게 되는 등 피검자들이 실험에 만족하고 있는 것으로 나타났다. 현재 생약 추출물은 식품 개발과 함께 의약품, 향미료, 화장품, 염료 등 다양한 상품으로 개발되고 있듯이, 본 연구에 활용한 생약 추출물도 모근의 혈액 순환을 촉진시켜 건강한 두피 유지와 모발 생성, 미용 생체 활성 물질로 응용할 여지가 크다고 생각된다. This study was conducted to examine the effect of herb extract on hair loss prevention and hair growth promotion. Four baldheaded men and two partly baldheaded women were selected as the subjects of this study. After they took the herb medicine and sprayed the herb extractant, the change of hair and scalp condition, hair roots, the number of hairs, and thickness of hairs was examined. It appeared that the amount of hair loss decreased gradually and new thin hairs were generated after receiving the treatment. Before the treatment, the average number of hairs in 1㎠ was approximately 44, which was below one third of that of normal people who had about 150 hairs in the 1㎠. After spraying and taking the herb extract for three months, the average number of hairs increased to 57, and it increased to 88 after another three months. It gradually improved and finally maintained healthy condition. According to their response, they felt confident in personal relations due to hair generation and growth. They were also concerned about beauty more than before. More than half of them were satisfied with the treatment. As herb extracts have being developed as various products such as medicine, perfume, cosmetics, and dyes, the herb extract which was used in this study can be applied to maintenance of healthy scalp, generation of hair, and active materials for beauty.