Bucillamine prevents cisplatin-induced ototoxicity through induction of glutathione and antioxidant genes

Kim, Se-Jin,Ho Hur, Joon,Park, Channy,Kim, Hyung-Jin,Oh, Gi-Su,Lee, Joon No,Yoo, Su-Jin,Choe, Seong-Kyu,So, Hong-Seob,Lim, David J,Moon, Sung K,Park, Raekil Nature Publishing Group 2015 Experimental and molecular medicine Vol.47 No.2

<P>Bucillamine is used for the treatment of rheumatoid arthritis. This study investigated the protective effects of bucillamine against cisplatin-induced damage in auditory cells, the organ of Corti from postnatal rats (P2) and adult Balb/C mice. Cisplatin increases the catalytic activity of caspase-3 and caspase-8 proteases and the production of free radicals, which were significantly suppressed by pretreatment with bucillamine. Bucillamine induces the intranuclear translocation of Nrf2 and thereby increases the expression of γ-glutamylcysteine synthetase (γ-GCS) and glutathione synthetase (GSS), which further induces intracellular antioxidant glutathione (GSH), heme oxygenase 1 (HO-1) and superoxide dismutase 2 (SOD2). However, knockdown studies of HO-1 and SOD2 suggest that the protective effect of bucillamine against cisplatin is independent of the enzymatic activity of HO-1 and SOD. Furthermore, pretreatment with bucillamine protects sensory hair cells on organ of Corti explants from cisplatin-induced cytotoxicity concomitantly with inhibition of caspase-3 activation. The auditory-brainstem-evoked response of cisplatin-injected mice shows marked increases in hearing threshold shifts, which was markedly suppressed by pretreatment with bucillamine <I>in vivo</I>. Taken together, bucillamine protects sensory hair cells from cisplatin through a scavenging effect on itself, as well as the induction of intracellular GSH.</P>

십이지장 궤양 환자와 만성 위염 환자의 염증 분포의 차이 및 H. pylori 집락도와 염증도의 상관관계

유광하,진춘조,박형석,이재동,김예리,윤성욱,이수인,윤호상 건국대학교 의과학연구소 1998 건국의과학학술지 Vol.8 No.-

An inflammatory difference between the gastric antrum, body and fundus was studied in 39 H.pylroi positive duodenal ulcer and 29 H. pylori chronic gastritis patients. Also we studied correlation of H. pylori colony count and inflammatory severity. Patients with newly diagnosed duodenal ulcer and chronic gastritis were endoscoped and two biopsy secimens each taken from the antrum, body and fundus. Although intestinal metaplasia in antrum and inflammatory activity in fundus were more severe in chronic gastritis patients. another inflammatory difference was equally likely to be found between two groups. There was no definite histological inflammatory difference between duodenal ulcer and chronic gastritis patients. A positive correlation was found between the number of H. pylori and the severity of inflammation.

한국과 미국의 노인복지법 비교 연구 : 한국의 노인복지법 개정과 관련된 추진 과제에 대한 제안

유성호 한국노인복지학회 2001 노인복지연구 Vol.14 No.-

In order to propose some comments related to the Amendments of the Older Koreans Act for the near future, this study analyzes the Older Americans Act and compares it with the Older Koreans Act. The comments proposed are as follows: First, service programs for the Korean elderly participating at senior centers shoed be provided universally not selectively and current paying-mechanism of fixed charge for some programs for not poor-group of elderly should be substituted by contributory basis. Second, aging service network for the comprehensive planning, finance, coordination, and delivery of aging services should be initiated and included in the Older Koreans Act. Third, the national level responsible for the matters related to the welfare for the aged should be escalated. Establishment of the Departrnent of Elder Affairs(tentative name) is suggested. Fourth, a national research center(institute on aging) conducting studies on the variety of issues on aging should be established and the results founded by the center should be used four the social policies for older Koreans. Fifth, elder rights protection acts should be initiated and included in the Older Koreans Act. To protect the elderly living in the community as well as in institutional. settings against elder abuse(physical and emotional), neglect, and material exploitation, elder abuse protection act should be initiated. Also the Ombudsman program which advocates for the residents of nursing homes, board and care homes, assisted living facilities and similar adult care facilities, should be initiated. Sixth, the Korean government's social policy for the elderly, known as `family support first, pubic support next policy', should be changed. A sizable, direct government spending on the welfare programs for the elderly should be made. First priority has given to the Korean traditional value of filial piety and encouragement of adult children to care of their older parents for the welfare policies of the older population. The traditional value, stressing the moral responsibility of adult children to care of their older parents, is not the best device for the welfare of the elderly. The Korean society has been changed dramatically in terms of culture and economy since last few decades.

고출력 레이저에 의한 이종금속 용접시험평가에 관한 연구

유영태,신호준,송성욱,모양우 朝鮮大學校 機械技術硏究所 2004 機械技術硏究 Vol.7 No.1

Laser welding techniques have been characterised for various materials. In this paper. the laser weldability of STS304 stainless steel and Inconel 600 at dissimilar metal welds using a continuous wave Nd:YAG laser are experimentally investigated. Inconel 600 is being used in a steam generator tubing of pressurized water reactor(PWR) exposed to some corrosion. Stress corrosion cracking can occur on this material. An experimental study was conducted to determine effects of welding parameters, on eliminating or reducing the extent welding zone formation at dissimilar metal welds and to optimize those parameters that have the most influence parameters such as focus length, power, beam speed, shielding gas, and wave length of laser were tested.

습기제거시스템의 성능에 관한 연구

유성연,정민호,홍정표,심국진,김광영 忠南大學校 産業技術硏究所 1997 산업기술연구논문집 Vol.12 No.2

The performance of moisture removal system is investigated analytically and experimentally. The program for the moist air calculation and for the condensing tube design are developed to examine the factors which affect moisture removal rate. Experimental apparatus comprises condensing tube, cooling system, vacuum pump, measurement and control devices. Thermoelectric cooling system and vapor compression refrigeration system are used to cool the air, and the performance of two systems are compared each other. Thermoelectric cooling system is compact and free from noise and vibration, but cooling rate of it is relatively slow compared to that of vapor compression refrigeration system. Air velocity, indoor temperature and humidity, temperature of coolant, size and material of condensing tube are found to affect the performance of moisture removal.

유료노인복지회관의 필요성에 관한 연구

유성호 한국사회복지정책학회 2004 사회복지정책 Vol.19 No.-

본 연구의 목적은 중산층 이상의 노년층이 증가하고 있음에도 불구하고 정부의 저소득층 노인 중심의 복지정책으로 정부는 물론이고 시장으로부터도 외면당하고 있는 중산노년층의 여가와 관련된 복지 욕구를 충족시키기 위한 방안으로 민간자본을 활용한 유료노인복지회관의 필요성을 조사하는데 있다. 인천광역시와 경기도 신도시에 각각 소재한 2곳의 노인복지회관을 이용하는 60세 이상 노인 305명을 대상으로 설문조사를 실시하여 빈도분석과 로지스틱회귀분석을 이용하여 자료를 분석하였다. 연구 결과, 첫째, 노인복지회관 이용노인들간의 사회적 신분(사회적 경험)과 사고방식이 비슷한 경우에 원만한 교류가 가능하다는 '가치동질성(value homophily)'의 타당성을 뒷받침하고 있다. 둘째, 우리나라 노인복지회관은 시설공간이 협소함에도 불구아고 이용노인이 너무 많기 때문에 제공되는 서비스가 다양하지 못하여 이응노인들의 복지욕구를 충족시켜 주지 못하고 있는 것으로 조사되었다. 셋째, 응답자 가운데 약 40%가 유료노인복지회관에 참여할 의사가 있다고 응답하였으며, 그들을 대상으로 참여하고자 하는 가장 큰 이유를 조사한 결과 약 70%가 '다양하고 수준 높은 양질의 서비스를 제공받기 위해서'라고 응답하여, 다양하고 수준 놓은 서비스에 대한 응답자의 욕구가 매우 강한 것으로 조사되었다. 넷째, 유료노인복지회관의 참여여부와 관련된 변인은 연령, 교육수준, 그리고 월평균 용돈으로, 연령이 적고, 교육수준이 높고, 월평균 용돈이 많은 노인일수록 유료노인복지회관에 참여할 의사가 있는 것으로 나타났다. 즉 학력수준이 놓고 경제력이 있는 젊은 중산층 이상의 노년층이 기존 노인복지회관에서 제공되는 서비스보다 다양하고 수준 높은 서비스를 기대하는 것으로 나타났다. 이러한 연구 결과와 노인집단의 사회경제적인 환경변화론 고려할 때, 증산층 이상의 노년층을 대상으로 운영되는 유료노인복지회관이 필요하다는 결론에 도달하였다. In spite of rapidly growing older middle-class population, government's welfare policies for the elderly are still focused on the poor groups of elderly. Therefore, the middle-class Korean elderly are alienated not only from the government but also from the industrial markets. The purpose of this study, therefore, was to examine the necessity of the for-profit senior center for the middle-class elderly in Korea. A sample of 305 Koreans aged 60 years and older participating at two senior centers located in Kyunggi province was analysed. The results of the study showed; first, it was verified that class-related disparities in social status, value, and life style hampered sound friendship formation among the center users. Second, it was found that the participants were not satisfied with the programs provided by the senior centers because of lack of physical space with full of users. Third, It was found that about 40% of the respondents were highly interested in participating at the for-profit senior center in order to be provided diverse programs with high level of standard. Finally, this study examined the factors related to the participation for-profit senior center. The results of logistic regression analysis showed that age, level of education and monthly pocket money were statistically significant. That is, the younger Korean elderly who had a higher level of education and were more financially secure were more likely to participate at the for-profit senior center. Considering the study results and socioeconomic changes among the older population in Korea, this study concluded that the for-profit senior center would be an alternative to fill the welfare needs of the middle-class elderly.

원격 영상 정보 시스템의 개발

김선호,유선국,김용구,박성욱,김성림,김남현 대한의료정보학회 1996 Healthcare Informatics Research Vol.2 No.1

압박 고 나사로 치료한 대퇴골 전자부 골절 : 고정 실패의 인자에 대한 분석 Analysis of Factors associated with Failure of Fixation

유찬훈,김홍태,변영수,남준모,조영호,문성건 대한골절학회 2002 대한골절학회지 Vol.15 No.4

목 적 : 압박 고 나사로 치료한 대퇴골 전자부 골절의 고정 실패에 영향을 미치는 요소에 대하여 분석하고자 하였다. 대상 및 방법 : 1995년 5월부터 2000년 7월까지 대퇴골 전자부 골절을 압박 고 나사를 이용하여 치료하고 1년 이상 추시가 가능했던 97예를 대상으로 하였다. 골절의 분류는 Jensen 분류법을, 골다공증의 정도는 Singh 지수를 이용하였다. 술후 방사선 검사에서 경체각, 정복 상태, 지연 나사의 대퇴골 두 내 위치, tip-apex distance(TAD) 및 지연 나사의 활강 거리를 측정하였고 고정 실패에 미치는 영향을 통계학적으로 분석하였다. 결 과 : 97예 중 17예(17%)에서 고정 실패가 발생하였고 그 중 지연 나사의 과도한 활강으로인한 경우가 15례(15.4%), 골두 천공 1얘, 10도 이상의 외반 변형 1예였다. 고정 실패의 발생과 80세 이상의 고령, 불안정성 골절, telescoping 정복, 원위 골편의 내측 혹은 전방 전위, 대퇴골 두 내 지연 나사의 위치와 밀접한 관계가 있었다. 결 론 : 압박 고 나사를 이용한 대퇴골 전자부 골절의 치료에서 고정 실패를 방지하기 위해서는 정확한 정복이 필요하고 대퇴골 두 내에서 지연 나사의 전방 위치를 피하는 것이 좋을 것으로 사료된다. Purpose : This study was performed to analyze the significant factors that may affect failure of fixation in trochanteric fractures of the femur treated with the compression hip screw. Materials and Methods : From May 1995 to July 2000, the authors analyzed 97 cases of trochanteric fracture of the femur treated with the compression hip screw and followed more than one year. We classified the fracture type by Jensen's method. We used Singh index for the degree of osteroporosis. In the post-operative radiograph, we checked neck-shaft angle, state of reduction, position of the lag screw within the femoral head, tip-apex distance, and sliding distance of the lag screw. The relationship between these factors and failure of fixation was statistically analyzed. Results : There were 17 cases(17.5%) of failure of fixation ; 15 cases(15.4%) of excessive sliding of the lag screw, 1 case (1%) of cutting out of the lag screw, and 1 case (1%) of valgus malunion. There were significant relationship between failure of fixation and old age over 80 , unstable fracture, telescoping reduction, anterior or medial displacement of the distal fragment, and anterior placement of the lag screw within the femoral head. Conclusion : Accurate reduction and avoidance of the placement of the lag screw in the anterior part of the femoral head were important factors to prevent failure of fixation in trochanteric fractures of the femur treated with the compression hip screw.

급성 뇌경색 환자에서 경색 위치가 심근 효소치 상승에 미치는 영향

윤경호,박현영,박성욱,장혁,김요식,조광호,유남진,김남호,오석규,정진원 圓光大學校 醫科學硏究所 2003 圓光醫科學 Vol.18 No.2

Staphylococcus aureus와 Coagulase-Negative Staphylococcus Species에 대한 Arbekacin의 시험관내 항균력

위성헌,강진한,허동호,이동건,김상일,김양리,최정현,김종현,유진흥,허재균,신완식,강문원 대한감염학회 2001 감염 Vol.33 No.4

Background : Most strains of methicillin-resistant Staphylococcus aureus (MRSA) now exhibit high-level resistance to various antibiotics, such as β -lactam antibiotics, aminoglycosides, macrolides, tetracyclines and quinolones. Recent reports describing the therapeutic failure of vancomycin for MRSA infections have arisen considerable concerns regarding the emergence of MRSA strains, which will require new therapeutic agents. Arbekacin, an aminoglycoside antibiotic, has antibacterial activity against both gram-positive and gram-negative bacteria and is stable in the presence of aminoglycoside inactivating enzymes produced by S. aureus. In this study, we compared the antibacterial activity of arbekacin with those of vancomycin, gentamicin, and amikacin against Staphylococcus aureus (S. aureus) and coagulase-negative staphylococci (CNS). Methods : For a collection of 549 S. aureus and 251 CNS isolates from three Catholic University Hospitals in Korea, minimum inhibitory concentrations (MICs) of arbekacin, vancomycin, amikacin and gentamicin were determined by agar dilution method using Mueller-Hinton agar according to NCCLS (National Committee for Clinical Laboratory Standards, USA)criteria. Results : Among 549 S. aureus isolates, 278 isolates were MRSA and 271 isolates were methicil sensitive S. aureus (MSSA). MIC50 & MIC90 of arbekacin against 549 S. aureus were 0.5 & 1 ㎍/mL, and MIC50 & MIC90 of vancomycin were 1 & 1 ㎍/mL. MIC of arbekacin against 549 S. aureus isolates ranges from 0.03 to 4 ㎍/mL, and MIC of vancomycin against 549 S. aureus ranges from 0.25 to 2 ㎍/mL. MIC90 of amikacin against 549 S. aureus was 32㎍/mL, and that of gentamicin was 128 ㎍/mL. MICs of amikacin and gentamicin were variable, ranging from 0.125 to 256, and otherwise arbekacin and vancomycin revealed relatively narrow range of MICs. MIC90 of arbekacin against 278 MRSA isolates & 271 MSSA were 1 & 0.5 ㎍/mL, and those of vancomycin against MRSA & MSSA were 1 & 1 ㎍/mL. MIC90 of amikacin against 278 MRSA & 271 MSSA isolates were 32 & 4 ㎍/mL, and that of gentamicin against MRSA & MSSA isolates were 128 & 32 ㎍/mL respectively. Among 251 CNS isolates, 122 isolates were MRCNS and 129 were MSCNS. MICSO & MIC90 of arbekacin against 251 CNS isolates were 0.25 & 2 ㎍/mL, and those of vancomycin were 1 & 2 ㎍/mL. MIC of arbekacin against 251 CNS isolates ranges from 0.015 to 32 ㎍/mL, and that of vancomycin isolates ranges from 0.25 to 2 ㎍/mL, MIC90 of arbekacin against 122 MRCNS & 129 MSCNS isolates were 2&0.3 ㎍/ML, and those of vancomycin were 2&s ㎍/ML. MIC90 of amikacin against 251 CNS isolates was 32 ㎍/ML, and that of gentamicin was 128 ㎍/ML for CNS. MIC90 of amikacin against 122 MRCNS & 129 MSCNS isolates were 128 & 8㎍/mL, and those of gentamicin ere 256 & 32 ㎍/mL. Conclusion : Considering above results, arbekacin can be useful agent against most strains of MRSA and MRCMS, which exhibit high-level resistance to amikacin and gentamicin. (Korea J Infect Dis 33:254~260, 2001)