Efficient and Precise Construction of Markerless Manipulations in the Bacillus subtilis Genome

Hao Jie Yu,Xin Yan,Wei Ling Shen,Yu Jia Shen,Ji Zhang,Shun Peng Li 한국미생물 · 생명공학회 2010 Journal of microbiology and biotechnology Vol.20 No.1

Sex Differences in Early and Late Recurrence after Liver Resection of Hepatocellular Carcinoma (A Multicenter Study from China)

( Jiong-jie Yu ),( Ju-dong Li ),( Xin-fei Xu ),( Zhen-li Li ),( Jun Han ),( Hao Xing ),( Han Wu ),( Jian-hong Zhong ),( Yi-sheng Huang ),( Ya- Hao Zhou ),( Ting-hao Chen ),( Hong Wang ),( Wei-min Gu ) 대한간학회 2018 춘·추계 학술대회 (KASL) Vol.2018 No.1

Aims: There is a striking sex difference in the incidence of hepatocellular carcinoma (HCC), with a strong predominance for males. However, the impact of sex on the incidence of recurrence after curative resection of HCC remains controversial. Herein, we assess sex differences in the risks of recurrence and mortality for patients undergone curative resection of HCC. Methods: Data from 1,435 HCC patients undergone curative resection (1,228 males and 207 females) between 2004 and 2014 at five institutions in China were retrospectively analyzed. Patients’ baseline characteristics, operative variables, and rates of early recurrence (≤ 2 years after resection), late recurrence (> 2 years) and cancer-specific mortality (CSM) were evaluated and compared. Multivariable competing-risks regression analyses were performed to identify predictors associated with CSM, early and late recurrence. Results: The early recurrence rates between males and females were similar (43.3% vs. 42.0%, P=0.728), but the late recurrence and CSM rates in males were higher when compared to females (17.2% vs. 11.2%, P=0.044; 42.8% vs. 34.3%, P=0.022). Multivariable competing-risks regression analyses revealed no sex difference in early recurrence; however, males had significantly higher late recurrence rate [hazard ratio (HR), 1.752; 95% CI, 1.145-2.682; P=0.010] and CSM rate (HR, 1.307; 95% CI, 1.015-1.683; P=0.038) than females. Conclusions: Males had significantly higher late recurrence and CSM rates after curative resection of HCC than females. This suggests postoperative surveillance for HCC recurrence be varied by sex, especially for patients without recurrence at 2 years after resection.

A Fast Voxel-based Method for Outlier Removal in Laser Measurement

Hao Chen,Yu Chen,Xu Zhang,Baiyuan Li,Xiaoqiang Liu,Xuefei Shi,Jie Shen 한국정밀공학회 2019 International Journal of Precision Engineering and Vol.20 No.6

Discrete data points are noncontinuous without structural information. In this paper, we propose a new fast outlier removal method via voxel-based surface propagation. The main technical components of our approach include (a) an efficient and simple spatial partitioning scheme and (b) a specially-designed surface propagation method. Numerical analyses indicate that our method is about 10 times faster than an existing method and significantly better than other two methods in terms of denoising accuracy. This provides an efficient solution to handling noisy laser-scanning data.

Bioaccumulation of Arsenic in Recombinant Escherichia coli Expressing Human Metallothionein

Yu-Jie Su,Jian-Qun Lin,Jian-Qiang Lin,Dong-Hui Hao 한국생물공학회 2009 Biotechnology and Bioprocess Engineering Vol.14 No.5

The recombinant Escherichia coli(E.coli) expressing human hepatic metallothionein_IA (hMT_IA) was constructed for bioaccumulation of Arsenic (As). The gene sequence of hMT_IA was modified for codon preference of E.coli and synthesized using chemical method. The vector of pGEX_4T_1 was used=and hMT_IA was expressed as the fusion protein with glutathione S-transferase (GST) tag. The bioaccumulation capability of arsenite compounds As(III) of the recombinant E.coli increased more than 3-fold from 76.3 to 319.6 μg/g dry cells compared with the control. The conditions of 50 μM of As(III) and low pHs were optimal for As(III) bioaccumulation. The heavy metals of Cd, Hg, and Zn inhibited As(III) bioaccumulation. The bioaccumulation reached 70% of the saturated value within 1 h. The recombinant E.coli will be useful in bioremediation of arsenic or other kinds of heavy metal contaminated water. The recombinant Escherichia coli(E.coli) expressing human hepatic metallothionein_IA (hMT_IA) was constructed for bioaccumulation of Arsenic (As). The gene sequence of hMT_IA was modified for codon preference of E.coli and synthesized using chemical method. The vector of pGEX_4T_1 was used=and hMT_IA was expressed as the fusion protein with glutathione S-transferase (GST) tag. The bioaccumulation capability of arsenite compounds As(III) of the recombinant E.coli increased more than 3-fold from 76.3 to 319.6 μg/g dry cells compared with the control. The conditions of 50 μM of As(III) and low pHs were optimal for As(III) bioaccumulation. The heavy metals of Cd, Hg, and Zn inhibited As(III) bioaccumulation. The bioaccumulation reached 70% of the saturated value within 1 h. The recombinant E.coli will be useful in bioremediation of arsenic or other kinds of heavy metal contaminated water.

BMB : Reports ; An inhibitory role of NEK6 in TGFβ/Smad signaling pathway

( Jie Zuo ),( Haijie Ma ),( Hao Cai ),( Yanhua Wu ),( Wei Jiang ),( Long Yu ) 생화학분자생물학회(구 한국생화학분자생물학회) 2015 BMB Reports Vol.48 No.8

The NEK6 (NIMA-related kinases 6) is reported to play po-tential roles in tumorigenesis. Although it is suggested to function in several cellular pathways, the underlying mechanism in tumorigenesis is still largely unknown. In the present study, we discovered interaction of NEK6 with Smad4, a key member of transforming growth factor beta (TGFβ) pathway. Over-expression of NEK6 in hepatocellular carcinoma (HCC) cell lines suppresses TGFβ- mediated transcription activity in a kinase activity-dependent manner. In addition, NEK6 suppresses the cell growth arrest induced by TGFβ. Mechanically, NEK6 blocks nuclear translocation of Smad4, which is essential for TGF β function. Moreover, we identified that NEK6 could be regulated by TGFβ and hypoxia. Our study sheds new light on the roles of NEK6 in canonical TGFβ/Smad pathway and tum-origenesis. [BMB Reports 2015; 48(8): 473-478]

Study on modulation of near infrared radiation based on plasma photonic crystal

Hao Jiong-Ju,Xie Xun,Gu Ke-Da,Liu Yu-Jie,Xia Lei,Yang Hong-Wei 한국물리학회 2020 Current Applied Physics Vol.20 No.8

In this paper, a plasma photonic crystal (PPC) for infrared radiation modulation which is composed of indium tin oxide (ITO) and plasma is proposed. The performance of plasma photonic crystal in near infrared radiation modulation is researched by transfer matrix method (TMM). The simulation results show that the near infrared radiation pass band can be adjusted by the changing of plasma frequency of plasma. The reflection to near infrared radiation by plasma photonic crystal increases with plasma frequency and that of absorption decreases. In addition, the modulation performance of the plasma photonic crystal at different incidence wave angles is also studied. The results show that the incident wave angles have little effect on the transmission of plasma photonic crystal in near infrared band. The reflection of the plasma photonic crystal to near infrared radiation decreases with increasing of the incident wave angle, but that of the absorption increases with the incident wave angle. Therefore, the proposed plasma photonic crystal has a potential application in tunable near infrared filter devices.

Seismic response of combined retaining structure with inclined rock slope

Yu-liang Lin,Jie Jin,Zhi-hao Jiang,Wei Liu,Hai-dong Liu,Rou-feng Li,Xiang Liu 국제구조공학회 2022 Structural Engineering and Mechanics, An Int'l Jou Vol.84 No.5

A gravity wall combined with an anchoring lattice frame (a combined retaining structure) is adopted at a typical engineering site at Dali-Ruili Railway Line China. Where, the combined retaining structure supports a soil deposit covering on different inclined rock slopes. With an aim to investigate and compare the effects of inclined rock slopes on the response of combined retaining structure under seismic excitation, three groups of shaking table tests are conducted. The rock slopes are shaped as planar surfaces inclined at angles of 20°, 30°, and 40° with the horizontal, respectively. The shaking table tests are supplemented by dynamic numerical simulations. The results regarding the horizontal acceleration response, vertical acceleration response, permanent displacement mode, and axial anchor force are comparatively examined. The acceleration response is more susceptible to outer structural profile of combined retaining structure than to inclined angle of rock slope. The permanent displacement decreases when the inclined angle of the rock slope increases within a range of 20°-40°. A critical inclined angle of rock slope exists within a range of 20°-40°, and induces the largest axial anchor force in the combined retaining structure.

A national survey on how to improve traditional Chinese medicine learning internationally: Perceptions from both teachers and students

Zhu Yu-Hang,Yan Wu,Hao Zhang,Yanfei Zhao,Yule Ren,Wei Hu,Pan Li,Huiyi Wang,Ying Wang,Boda Zhang,Yujie Wang,Hongwen Yuan,Jiping Zhang,Wenqiang Li,Yufang Zhao,Jie Zhao,Yedong Shi,Yanye Shu,Xiao Zhang,Ya 한국한의학연구원 2022 Integrative Medicine Research Vol.11 No.4

Background With the increasing popularity of traditional Chinese medicine (TCM) by the global community, how to teach basic knowledge of TCM to international students and improve the teaching quality are important issues for teachers of TCM. The present study was to analyze the perceptions from both students and teachers on how to improve TCM learning internationally. Methods A cross-sectional national survey was conducted at 23 universities/colleges across China. A structured, self-reported on-line questionnaire was administered to 34 Chinese teachers who taught TCM course in English and to 1016 international undergraduates who were enrolled in the TCM course in China between 2017 and 2021. Results Thirty-three (97.1%) teachers and 900 (88.6%) undergraduates agreed Chinese culture should be fully integrated into TCM courses. All teachers and 944 (92.9%) undergraduates thought that TCM had important significance in the clinical practice. All teachers and 995 (97.9%) undergraduates agreed that modern research of TCM is valuable. Thirty-three (97.1%) teachers and 959 (94.4%) undergraduates thought comparing traditional medicine in different countries with TCM can help the students better understand TCM. Thirty-two (94.1%) teachers and 962 (94.7%) undergraduates agreed on the use of practical teaching method with case reports. From the perceptions of the undergraduates, the top three beneficial learning styles were practice (34.3%), teacher's lectures (32.5%), case studies (10.4%). The first choice of learning mode was attending to face-to-face teaching (82.3%). The top three interesting contents were acupuncture (75.5%), Chinese herbal medicine (63.8%), and massage (55.0%). Conclusion To improve TCM learning among international undergraduates majoring in conventional medicine, integration of Chinese culture into TCM course, comparison of traditional medicine in different countries with TCM, application of the teaching method with case reports, and emphasization of clinical practice as well as modern research on TCM should be fully considered.

Preferential Induction of CYP1A1 over CYP1B1 in Human Breast Cancer MCF-7 Cells after Exposure to Berberine

Wen, Chun-Jie,Wu, Lan-Xiang,Fu, Li-Juan,Shen, Dong-Ya,Zhang, Xue,Zhang, Yi-Wen,Yu, Jing,Zhou, Hong-Hao Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.1

Estrogens are considered the major breast cancer risk factor, and the carcinogenic potential of estrogens might be attributed to DNA modification caused by derivatives formed during metabolism. $17{\beta}$-estradiol ($E_2$), the main steroidal estrogen present in women, is metabolized via two major pathways: formation of 2-hydroxyestradiol (2-OH $E_2$) and 4-hydroxyestradiol ($4-OH\;E_2$) through the action of cytochrome P450 (CYP) 1A1 and 1B1, respectively. Previous reports suggested that $2-OH\;E_2$ has putative protective effects, while $4-OH\;E_2$ is genotoxic and has potent carcinogenic activity. Thus, the ratio of $2-OH\;E_2/4-OH\;E_2$ is a critical determinant of the toxicity of $E_2$ in mammary cells. In the present study, we investigated the effects of berberine on the expression profile of the estrogen metabolizing enzymes CYP1A1 and CYP1B1 in breast cancer MCF-7 cells. Berberine treatment produced significant induction of both forms at the level of mRNA expression, but with increased doses produced 16~ to 52~fold greater induction of CYP1A1 mRNA over CYP1B1 mRNA. Furthermore, berberine dramatically increased CYP1A1 protein levels but did not influence CYP1B1 protein levels in MCF-7 cells. In conclusion, we present the first report to show that berberine may provide protection against breast cancer by altering the ratio of CYP1A1/CYP1B1, could redirect $E_2$ metabolism in a more protective pathway in breast cancer MCF-7 cells.

Cross-border Tourism Cooperation in The Context of China-Myanmar Economic Corridor : The Case of the Dehong

Wei Zong,Jie Yu,Hao Zhang,Chengcen Zhang 한국국회학회 2020 한국과 세계 Vol.2 No.2

21세기 초부터 중국의 해외관광산업이 많이 활발해졌고, 운남성도 이러한 배경 하에 주변국가와 관광산업분야에서 협력의 폭과 깊이를 넓힐 수 있었다. 중국-미얀마 경제회랑은 중국이 설립 된 두 번째 무역회랑이며 이는 란창-메콩 지역발전에 중요한 역할을 하고 있다. 학술계는 운남 및 미얀마 양측의 관광산업을 중심으로 한 협력이 더욱더 활성화 될 거라고 전망하고 있다. 본 논문은 중국-미얀마 경제 회랑건설을 기반으로 중국 Dehong지역과 미얀마 간의 관광산업협력 현황 및 협력 메커니즘, 인프라 구축, 국경 간 관광 상품 개발 및 관광서비스 등을 분석하였고, 또한 Dehong과 미얀마 간 관광 협력에 적합한 친환경적인 협력모델을 탐색하였다. Since the 21st century, the ways of cross-border and cross-national tourism in China’s borders have been diversified increasingly, which has expanded the breadth and depth of Yunnan’s foreign cooperation. The China-Myanmar Economic Corridor is the second trade corridor established between China and unilateral countries. It plays an essential role in the construction of the Lancang-Mekong Regional Economic Corridor. Based on the construction of the China-Myanmar Economic Corridor, this article analyzes the statuses and issues of cross-border tourism cooperation between Myanmar and China’s Dehong Prefecture, which mainly includes the cooperation mechanism, infrastructure construction, cross-border tourism product development, and tourism service levels in Dehong Prefecture, so that to explore a suitable cross-border tourism cooperation model with ecological pattern in this region. The research aims to enrich the theories of China’s border and cross-border tourism development and provide significant references for promoting cross-border tourism cooperation in Dehong Prefecture under the background of the China-Myanmar Economic Corridor.