Prognostic Significance of Tumor Mutation Burden among Patients with Non-small Cell Lung Cancer Who Received Platinum-based Adjuvant Chemotherapy: An Exploratory Study

Wei-Xi Shen,Guang-Hua Li,Yu-Jia Li,Peng-Fei Zhang,Jia-Xing Yu,Di Shang,Qiu-Shi Wang 대한암예방학회 2023 Journal of cancer prevention Vol.28 No.4

This study aimed to investigate the prognostic significance of tumor mutation burden (TMB) among patients with non-small cell lung cancer (NSCLC) who received platinum-based adjuvant chemotherapy. Tumor tissue specimens after surgical resection were collected for DNA extraction. Somatic mutation detection and TMB analysis were conducted using next-generation sequencing (NGS). Recurrence status of the patients was assessed in the hospital during the adjuvant chemotherapy period, and long-term survival data of patients were obtained by telephone follow-up. Univariate analysis between TMB status and prognosis was carried out by survival analysis. A retrospective review of 78 patients with non-squamous NSCLC who received platinum-based adjuvant chemotherapy showed a median disease-free survival of 3.6 years and median overall survival (OS) of 5.3 years. NGS analysis exhibited that the most common mutated somatic genes among the 78 patients were tumor suppressor protein p53 (TP53), epidermal growth factor receptor, low-density lipoprotein receptor related protein 1B, DNA methyltransferase 3 alpha and FAT atypical cadherin 3, and their prevalence was 56.4%, 48.7%, 37.2%, 30.7%, and 25.6%, respectively. TMB status was divided into TMB-L (≤ 4.5/Mb) and TMB-H (> 4.5/Mb) based on the median TMB threshold. Relevance of TMB to prognosis suggested that the median OS of patients with TMB-L was significantly longer than that of patients with TMB-H (NR vs. 4.6, P = 0.014). Higher TMB status conferred a worse implication on OS among patients with non-squamous NSCLC who received platinum-based adjuvant chemotherapy.

Efficient and Precise Construction of Markerless Manipulations in the Bacillus subtilis Genome

Hao Jie Yu,Xin Yan,Wei Ling Shen,Yu Jia Shen,Ji Zhang,Shun Peng Li 한국미생물 · 생명공학회 2010 Journal of microbiology and biotechnology Vol.20 No.1

Present Situation and Prospects of Sericulture in China

Shen, Xing-Jia,Ye, Xia-Yu,Guo, Xi-Jie Korean Society of Sericultural Science 2006 International Journal of Industrial Entomology Vol.13 No.2

Since 1970, China has become the biggest cocoon producer in the world, and made the highest historical record of cocoon output for 759,800 tons in 1995. However, in 1996 cocoon production reduced sharply to 470,900 tons. After a ten-year adjustment and reform, sericultural areas have shifted from developed regions to developing regions and from the east to the west. From 2000, the cocoon output has started to increase restoringly. By 2004 it recovered to 547,091 tons. With the development of market economy, sericulture management has been changed, including mulberry fields concentrated to the specializated households and cooperatives, cocoons produced in larger scale instead of individuals, Silkworm egg producing enterprises gradually changed into non-governmental joint-stock ones. The mechanism of market cocoon price has been gradually established. The management model of combination of trade, industry and agriculture is pushing and improving. It is the fruit of modern science and technology, especially sericultural basic research, that provides China's sericulture with the opportunity and vital force. China's sericulture, therefore, will continue to develop steadily in future.

Remote Monitoring of Heading Rice Growing and Nitrogen Content Based on UAV Images

Yu Zhang,Zhongbin Su,Weizheng Shen,Renshan Jia,Jiling Luan 보안공학연구지원센터 2016 International Journal of Smart Home Vol.10 No.7

Rice heading is the critical stage of the growth of rice, rice plants are relatively large, field canopy, poor resistance, and coincided with the high temperature and rainy weather, pests and diseases are more prone period. Therefore, the use of UAV to monitor the growth status of rice, easy to understand rice growth and nutritional status, in order to achieve high-quality, high yield, efficient purpose. This article is the field experiments under different nitrogen levels, using UAV monitoring multispectral images of rice, by reference remote sensing spectral indices, derived green normalized difference vegetation index (GNDVI) relative to other spectral index is more suitable for rice field biomass inversion modeling. At the same time, this study is based on the inversion model, implemented the rice growing and nitrogen content graded by ISODATA methods in ENVI. Achieved classification on rice growth and nitrogen remote sensing thematic map in ArcGIS, to provide timely and accurate information for rice seedling diagnosis and management decision, has reached the purpose of rice production precise management.

Influence of Polycaprolactone/Collagen Ratio and Microstructure of FibrousMembranes on Mechanical Properties and Anti-Platelet Properties

Jia-Shuai Zhang,Hui-Lin Mo,Shun-Yang Shen,Du-Han Zhang,Wei Zhang,Yu Ren,Mei-Xian Li 한국고분자학회 2024 폴리머 Vol.48 No.1

In this paper, polycaprolactone (PCL)/collagen (COL) nanofibrous membranes were prepared by electrospinning to study their mechanical properties as well as biocompatibility. The surface morphology was characterized by scanning electron microscopy (SEM) to access the effect of microstructure on the mechanical properties, cytocompatibility, and enzymatic degradation behaviors. In addition, the effect of hydrophilicity of membranes on the antithrombotic properties was also investigated. The results show that tensile strength of PCL/COL10 is higher than that of other samples due to smaller fiber diameters and tight spatial interlacing of PCL/COL10 membranes. In addition, the hydrophobic surface of PCL becomes hydrophilic after the addition of collagen, which affect platelet adhesion and cytocompatibility of nanofibrous membrane samples. The addition of collagen could promote the cell proliferation as well as the enzymatic degradation rate. Overall, PCL/COL10 has good morphology, the best mechanical properties, appropriate degradation rate, and the best antithrombotic effect as well as biocompatibility.

Angiotensin I Converting Enzyme (ACE) Inhibitory Activity and Antihypertensive Effects of Grass Carp Peptides

Wang Shen,Lin Li-mei,Wu Yong-ning,Fang Min,Yu Ya-qin,Zhou Jia,Gong Zhi-yong 한국식품과학회 2014 Food Science and Biotechnology Vol.23 No.5

In vitro and in vivo antihypertensive effects ofgrass carp peptides (GCP) were investigated. The aminoacid composition and the angiotensinconverting enzymeinhibitory activity were evaluated using HPLC. GCP wasadministrated to spontaneously hypertensive rats (SHRs)and Wistar Kyoto rats (WKY) by single and long-termadministration before blood pressure measurements. Plasma levels of angiotensin II (Ang II), rennin (RA), nitricoxide (NO), and plasma angiotensin I converting enzyme(ACE) activity were measured. The GCP molecular weightwas between 725 and 1,228 Da with high levels of Leu,Asp, Phe, Gly, and Pro. The in vitro IC50 value was 0.23mg/mL. GCP lowered SHR blood pressure dose and timedependently. Little change occurred in WKY. The plasmalevel of Ang II and the ACE activity decreased. SHR RAand NO concentrations in plasma increased. Theantihypertensive effect of GCP was associated with NOregulation and the rennin-angiotensin system.

Serum Tumor Marker Levels might have Little Significance in Evaluating Neoadjuvant Treatment Response in Locally Advanced Breast Cancer

Wang, Yu-Jie,Huang, Xiao-Yan,Mo, Miao,Li, Jian-Wei,Jia, Xiao-Qing,Shao, Zhi-Min,Shen, Zhen-Zhou,Wu, Jiong,Liu, Guang-Yu Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.11

Background: To determine the potential value of serum tumor markers in predicting pCR (pathological complete response) during neoadjuvant chemotherapy. Materials and Methods: We retrospectively monitored the pro-, mid-, and post-neoadjuvant treatment serum tumor marker concentrations in patients with locally advanced breast cancer (stage II-III) who accepted pre-surgical chemotherapy or chemotherapy in combination with targeted therapy at Fudan University Shanghai Cancer Center between September 2011 and January 2014 and investigated the association of serum tumor marker levels with therapeutic effect. Core needle biopsy samples were assessed using immunohistochemistry (IHC) prior to neoadjuvant treatment to determine hormone receptor, human epidermal growth factor receptor 2(HER2), and proliferation index Ki67 values. In our study, therapeutic response was evaluated by pCR, defined as the disappearance of all invasive cancer cells from excised tissue (including primary lesion and axillary lymph nodes) after completion of chemotherapy. Analysis of variance of repeated measures and receiver operating characteristic (ROC) curves were employed for statistical analysis of the data. Results: A total of 348 patients were recruited in our study after excluding patients with incomplete clinical information. Of these, 106 patients were observed to have acquired pCR status after treatment completion, accounting for approximately 30.5% of study individuals. In addition, 147patients were determined to be Her-2 positive, among whom the pCR rate was 45.6% (69 patients). General linear model analysis (repeated measures analysis of variance) showed that the concentration of cancer antigen (CA) 15-3 increased after neoadjuvant chemotherapy in both pCR and non-pCR groups, and that there were significant differences between the two groups (P=0.008). The areas under the ROC curves (AUCs) of pre-, mid-, and post-treatment CA15-3 concentrations demonstrated low-level predictive value (AUC=0.594, 0.644, 0.621, respectively). No significant differences in carcinoembryonic antigen (CEA) or CA12-5 serum levels were observed between the pCR and non-pCR groups (P=0.196 and 0.693, respectively). No efficient AUC of CEA or CA12-5 concentrations were observed to predict patient response toward neoadjuvant treatment (both less than 0.7), nor were differences between the two groups observed at different time points. We then analyzed the Her-2 positive subset of our cohort. Significant differences in CEA concentrations were identified between the pCR and non-pCR groups (P=0.039), but not in CA15-3 or CA12-5 levels (p=0.092 and 0.89, respectively). None of the ROC curves showed underlying prognostic value, as the AUCs of these three markers were less than 0.7. The ROC-AUCs for the CA12-5 concentrations of inter-and post-neoadjuvant chemotherapy in the estrogen receptor negative HER2 positive subgroup were 0.735 and 0.767, respectively. However, the specificity and sensitivity values were at odds with each other which meant that improving either the sensitivity or specificity would impair the efficiency of the other. Conclusions: Serum tumor markers CA15-3, CA12-5, and CEA might have little clinical significance in predicting neoadjuvant treatment response in locally advanced breast cancer.

Review : Present Situation and Prospects of Sericulture in China

( Xing Jia Shen ),( Xia Yu Ye ),( Xi Jie Guo ) 한국잠사학회 2006 International Journal of Industrial Entomology Vol.13 No.2

Since 1970, China has become the biggest cocoon producer in the world, and made the highest historical record of cocoon output for 759,800 tons in 1995. However, in 1996 cocoon production reduced sharply to 470,900 tons. After a ten-year adjustment and reform, sericultural areas have shifted from developed regions to developing regions and from the east to the west. From 2000, the cocoon output has started to increase restoringly. By 2004 it recovered to 547,091 tons. With the development of market economy, sericulture management has been changed, including mulberry fields concentrated to the specializated households and cooperatives, cocoons produced in larger scale instead of individuals, Silkworm egg producing enterprises gradually changed into non-governmental joint-stock ones. The mechanism of market cocoon price has been gradually established. The management model of combination of trade, industry and agriculture is pushing and improving. It is the fruit of modern science and technology, especially sericultural basic research, that provides China`s sericulture with the opportunity and vital force. China`s sericulture, therefore, will continue to develop steadily in future.

Access-Authorizing and Privacy-Preserving Auditing with Group Dynamic for Shared Cloud Data

( Wenting Shen ),( Jia Yu ),( Guangyang Yang ),( Yue Zhang ),( Zhangjie Fu ),( Rong Hao ) 한국인터넷정보학회 2016 KSII Transactions on Internet and Information Syst Vol.10 No.7

Cloud storage is becoming more and more popular because of its elasticity and pay-as-you-go storage service manner. In some cloud storage scenarios, the data that are stored in the cloud may be shared by a group of users. To verify the integrity of cloud data in this kind of applications, many auditing schemes for shared cloud data have been proposed. However, all of these schemes do not consider the access authorization problem for users, which makes the revoked users still able to access the shared cloud data belonging to the group. In order to deal with this problem, we propose a novel public auditing scheme for shared cloud data in this paper. Different from previous work, in our scheme, the user in a group cannot any longer access the shared cloud data belonging to this group once this user is revoked. In addition, we propose a new random masking technique to make our scheme preserve both data privacy and identity privacy. Furthermore, our scheme supports to enroll a new user in a group and revoke an old user from a group. We analyze the security of the proposed scheme and justify its performance by concrete implementations.

Factors influencing success and safety of AED retrieval in out of hospital cardiac arrests in Singapore

NG Jonathan Shen You,Ho Reuben Jia Shun,Yu Jae Yong,Ng Yih Yng 사단법인 한국응급구조학회 2022 한국응급구조학회지 Vol.26 No.2

Purpose: Automated External Defibrillator (AED) usage in Out of Hospital Cardiac Arrests (OHCAs) improves survival for patients. In Singapore, public AEDs are protected by locked boxes with a ‘break glass’ mechanism to deter theft. Community responders have sustained injuries while breaking glass to retrieve AEDs. This unprecedented study aims to elucidate factors influencing successful retrieval of an AED and document the prevalence of injuries. Methods: A survey was created and distributed. Participants must have responded to a OHCA in the past 12 months. Comparison tests were performed with Fischer-Freeman-Halton Exact test or Pearson chi square test at 5% significance levels, and multiple logistic regression with a logit link function. Results: Eighty eight participants were eligible. The success of retrieving an AED is not impacted by occupation, age, gender nor time. Participants who responded to an OHCA because of activation by the myResponder App were more likely to retrieve an AED successfully. (AOR 11.111, 95% CI: 2.141-58.824) Conclusion: Use of myResponder mobile application is associated with greater success of retrieving an AED. Successful retrieval of AED is not impacted by time, gender, age, or occupation of the responder. Community responders in Singapore remain motivated to respond to cardiac arrests despite risk of injury.