A national survey on how to improve traditional Chinese medicine learning internationally: Perceptions from both teachers and students

Zhu Yu-Hang,Yan Wu,Hao Zhang,Yanfei Zhao,Yule Ren,Wei Hu,Pan Li,Huiyi Wang,Ying Wang,Boda Zhang,Yujie Wang,Hongwen Yuan,Jiping Zhang,Wenqiang Li,Yufang Zhao,Jie Zhao,Yedong Shi,Yanye Shu,Xiao Zhang,Ya 한국한의학연구원 2022 Integrative Medicine Research Vol.11 No.4

Background With the increasing popularity of traditional Chinese medicine (TCM) by the global community, how to teach basic knowledge of TCM to international students and improve the teaching quality are important issues for teachers of TCM. The present study was to analyze the perceptions from both students and teachers on how to improve TCM learning internationally. Methods A cross-sectional national survey was conducted at 23 universities/colleges across China. A structured, self-reported on-line questionnaire was administered to 34 Chinese teachers who taught TCM course in English and to 1016 international undergraduates who were enrolled in the TCM course in China between 2017 and 2021. Results Thirty-three (97.1%) teachers and 900 (88.6%) undergraduates agreed Chinese culture should be fully integrated into TCM courses. All teachers and 944 (92.9%) undergraduates thought that TCM had important significance in the clinical practice. All teachers and 995 (97.9%) undergraduates agreed that modern research of TCM is valuable. Thirty-three (97.1%) teachers and 959 (94.4%) undergraduates thought comparing traditional medicine in different countries with TCM can help the students better understand TCM. Thirty-two (94.1%) teachers and 962 (94.7%) undergraduates agreed on the use of practical teaching method with case reports. From the perceptions of the undergraduates, the top three beneficial learning styles were practice (34.3%), teacher's lectures (32.5%), case studies (10.4%). The first choice of learning mode was attending to face-to-face teaching (82.3%). The top three interesting contents were acupuncture (75.5%), Chinese herbal medicine (63.8%), and massage (55.0%). Conclusion To improve TCM learning among international undergraduates majoring in conventional medicine, integration of Chinese culture into TCM course, comparison of traditional medicine in different countries with TCM, application of the teaching method with case reports, and emphasization of clinical practice as well as modern research on TCM should be fully considered.

PRESENTATION OF BIOACTIVE EPITOPES WITH FREE N-TERMINI ON SELF-ASSEMBLING PEPTIDE NANOFIBERS

YANFEI LIU,XIAOJUN ZHAO 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2011 NANO Vol.6 No.1

Branched self-assembling peptides bearing epitopes with free N-termini were designed. A lysine residue was used as branch point to present more than one epitopes in a single peptide. Atomic force microscope, circular dichroism and Fourier transform infrared spectroscopy data indicate that the N-terminus attached epitope sequences do not prevent the formation of the β-sheets and the self-assembling of these peptides into stable nanofibers in aqueous solutions. Rheology experiments show that these peptides could form self-supporting scaffolds once electrostatic repulsions were screened by electrolytes. Fluorescence spectra measurements upon binding of FITC-avidin to surfaces of nanofibers were performed to investigate the effect of charged aspartic acid residues in RGD epitopes at the lysine branching on packing and accessibility of the epitopes. Results show that the electrostatic interaction between hydrophilic side chains at branching and nanofiber surfaces may significantly affect the conformational freedom and accessibility of the epitopes at the periphery of the nanofibers. Cell entrapment experiments reveal that the attached RGD epitopes with free N-termini are biological active.

Effect of Start/stop Position Distribution on Residual Stresses in the Multi-pass Welded 12Cr1MoV/P91 Dissimilar pipe

Yanping Zhao,Jianming Gong,Yanfei Wang,Gao Wei 한국강구조학회 2014 International Journal of Steel Structures Vol.14 No.3

Welding residual stresses between 12Cr1MoV and P91 steel pipes were analyzed by experiment and finite element method(FEM). Both measured hoop and axial stresses are in general agree with the simulation results. Meanwhile, to examine the effectof start/stop welding position on residual stresses, a simulation model whose welding passes all started at the same location duringmulti-pass welding was compared with a model whose start/stop positions were uniformly distributed along circumferentialdirection of the dissimilar pipe. The results show that higher residual stresses occurred near the superimposed start/stop positionthan the steady region when calculated by the model whose welding passes all started at the same location. Whereas, start/stopwelding positions which are distributed uniformly along the circumferential direction of the pipe can significantly relieve thiseffect, correspondingly leading to a more uniform residual stress distribution around the multi-pass welded dissimilar pipe.

Genotoxicity and subchronic toxicological study of a novel ginsenoside derivative 25-OCH₃-PPD in beagle dogs

Li, Wei,Zhang, Xiangrong,Ding, Meng,Xin, Yanfei,Xuan, Yaoxian,Zhao, Yuqing The Korean Society of Ginseng 2019 Journal of Ginseng Research Vol.43 No.4

Background: Ginsenosides have been widely used clinically for many years and were regarded as very safe. However, a few researches on the toxicities of these kinds of agents showed that some ginsenosides may have side-effect on the rats or dogs. So it is extremely necessary to further clarify the potential toxicity of ginsenosides. This study was carried out to investigate long-term toxicity and genotoxicity of 25-methoxydammarane-3, 12, 20-triol ($25-OCH_3-PPD$), a new derivative of ginsenoside, in beagle dogs. Methods: Twenty-four beagle dogs were divided randomly into four treatment groups and repeatedly orally administered with $25-OCH_3-PPD$ capsule at 60, 120, and 240 mg/kg/day for 91 consecutive days. Ames, micronucleus, and chromosomal aberration tests were established to analyze the possible genotoxicity of $25-OCH_3-PPD$. Results: There was no $25-OCH_3-PPD$einduced systemic toxicity in beagle dogs at any doses. The level of $25-OCH_3-PPD$ at which no adverse effects were observed was found to be 240 mg/kg/day. The result of Ames test showed that there was no significant increase in the number of revertant colonies of $25-OCH_3-PPD$ administrated groups compared to the vehicle control group. There were also no significant differences between $25-OCH_3-PPD$ administrated groups at all dose levels and negative group in the micronucleus test and chromosomal aberration assay. Conclusion: The highest dose level of $25-OCH_3-PPD$ at which no adverse effects were observed was found to be 240 mg/kg per day, and it is not a genotoxic agent either in somatic cells or germs cells. $25-OCH_3-PPD$ is an extremely safe candidate compound for antitumor treatment.

Genotoxicity and subchronic toxicological study of a novel ginsenoside derivative 25-OCH₃-PPD in beagle dogs

Wei Li,Xiangrong Zhang,Meng Ding,Yanfei Xin,Yaoxian Xuan,Yuqing Zhao 고려인삼학회 2019 Journal of Ginseng Research Vol.43 No.4

Background: Ginsenosides have been widely used clinically for many years and were regarded as very safe. However, a few researches on the toxicities of these kinds of agents showed that some ginsenosides may have side-effect on the rats or dogs. So it is extremely necessary to further clarify the potential toxicity of ginsenosides. This study was carried out to investigate long-term toxicity and genotoxicity of 25-methoxydammarane-3, 12, 20-triol (25-OCH₃-PPD), a new derivative of ginsenoside, in beagle dogs. Methods: Twenty-four beagle dogs were divided randomly into four treatment groups and repeatedly orally administered with 25-OCH₃-PPD capsule at 60, 120, and 240 mg/kg/day for 91 consecutive days. Ames, micronucleus, and chromosomal aberration tests were established to analyze the possible genotoxicity of 25-OCH₃-PPD. Results: There was no 25-OCH₃-PPD-induced systemic toxicity in beagle dogs at any doses. The level of 25-OCH₃-PPD at which no adverse effects were observed was found to be 240 mg/kg/day. The result of Ames test showed that there was no significant increase in the number of revertant colonies of 25-OCH₃- PPD administrated groups compared to the vehicle control group. There were also no significant differences between 25-OCH₃-PPD administrated groups at all dose levels and negative group in the micronucleus test and chromosomal aberration assay. Conclusion: The highest dose level of 25-OCH₃-PPD at which no adverse effects were observed was found to be 240 mg/kg per day, and it is not a genotoxic agent either in somatic cells or germs cells. 25-OCH₃-PPD is an extremely safe candidate compound for antitumor treatment.

Proteomic Analysis on Exosomes Derived from Patients’ Sera Infected with Echinococcus granulosus

Wen Wang,Xiaojing Zhou,Fang Cui,Chunli Shi,Yulan Wang,Yanfei Men,Wei Zhao,Jiaqing Zhao 대한기생충학ㆍ열대의학회 2019 The Korean Journal of Parasitology Vol.57 No.5

Cystic echinococcosis (CE), a zoonotic disease caused by Echinococcus granulosus at the larval stage, predominantly develops in the liver and lungs of intermediate hosts and eventually results in organ malfunction or even death. The interaction between E. granulosus and human body is incompletely understood. Exosomes are nanosized particles ubiquitously present in human body fluids. Exosomes carry biomolecules that facilitate communication between cells. To the best of our knowledge, the role of exosomes in patients with CE is not reported. Here, we isolated exosomes from the sera of patients with CE (CE-exo) and healthy donors and subjected them to liquid chromatography-tandem mass spectrometry analysis. Proteomic analysis identified 49 proteins specifically expressed in CE-exo, including 4 proteins of parasitic origin. The most valuable parasitic proteins included tubulin alpha-1C chain and histone H4. And 8 proteins were differentially regulated in CE-exo (fold change>1.5), as analyzed with bioinformatic methods such as annotation and functional enrichment analyses. These findings may improve our understanding about the interaction between E. granulosus and human body, and may contribute to the diagnosis and prevention of CE.