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Escherichia coli(M15)을 이용한 serotonin N-acetyltransferase 유전자의 발현
김용만,정미영,윤경식,진병관,백행운,조용호,백형환 慶熙大學校 1997 論文集 Vol.26 No.-
AA-NAT cDNA was obtained by RT-PCR technique from total RNA of rat sacrified at night(02:00am). pCRNAT was cloned using the pCRII vector with insertion of AA-NAT cDNA(about 1.4 kb) at EcoRI site. For the expression of the gene, pQECNAT was subcloned, in which the coding region of AA-NAT was inserted into expression vector pQE30 at BamHI and HindIII sites. According to the experimental results, Escherichia coli strain M15, transformed by the expession vector pQECNAT, was selected as a host to express AA-NAT gene with the induction of isopropyl β-D-thiogalactoside(IPTG). Optimal condition for the expression of AA-NAT gene was achieve from the experimental results, showing that the expression of the protein was inducible much 19.800 ± 2,200 dpm per ml of culture volume in 4 hours at the concentration of 2 mM IPTG. Partial purification through the affinity column(Ni-NTA agarose) binding to the continuous 6 histidine residues of protein resulted in 5 times more increase in the specific activity of AA-NAT than that of the homogenate of bacterial pellet. These experimental results will provide basic data in further study for the enzymatic kinetics and antibody production of AA-NAT.
이종범,봉종헌,조하만 江原大學校 附設 環境硏究所 1990 環境硏究 Vol.7 No.-
Local climatic change due to the artificial lakes formed by dams was studied for 5 areas in Korea. At the lake side station there were remarkable increase of frequency of fog and decreases of sunshine du-ration and summertime air temperature. At the down stream area of dam. for frequency was increased while sunshine duration was decreased as those at the lake side. Analysis of hourly solar radiation and cloud amount data shows that at Chunchon. amount of solar radiation is less and cloud amount is more than those at the iniand areas in the morning time in autumn. The reason for these considered to be due to the evaporation from the relatively warm water surface discharged from the Lake soyang in autumn.
개 대뇌겉질에서 Platelet-Derived Growth Factor α-Receptor의 출생 후 발달에 관한 면역조직화학적 연구
윤영,안병수,김인정,양경철,박선홍,김기훈,박도영,김장만,문정석,장인엽,조하영 조선대학교 부설 의학연구소 2002 The Medical Journal of Chosun University Vol.27 No.1
Background and Objectives : The localization of platelet-derived growth factor-α receptor (PDGF-α R) was commonly restricted to oligodendrocyte progenitors during late embryonic and postnatal development. However, several studies recently demonstrated that mature neurons could also synthesize PDGF-α, Materials and Methods : In the present study, to analyze the distributional pattern of PDGF-αR during postnatal development of the canine cerebral cortex, we used immunohistochemistry on sections of canine brain tissue. Results : We found that neurons of various regions of cerebral cortex exhibited the immunoreactivity to PDGF-αR as early as postnatal day 0, and slightly decreased after postnatal day 14. Whereas neuronal PDGF-αR were maintained at all ages, the oligodendroglia-like expression of PDGF-αR could not be confirmed. Conclusion : The localization of PDGF-αR in immature and mature neurons supports the several roles of PDGF during development, protection and survival of neurons.
Aspergillus Tracheobronchitis in a Mild Immunocompromised Host
Cho, Byung Ha,Oh, Youngmin,Kang, Eun Seok,Hong, Yong Joo,Jeong, Hye Won,Lee, Ok-Jun,Chang, You-Jin,Choe, Kang Hyeon,Lee, Ki Man,An, Jin-Young The Korean Academy of Tuberculosis and Respiratory 2014 Tuberculosis and Respiratory Diseases Vol.77 No.5
Aspergillus tracheobronchitis is a form of invasive pulmonary aspergillosis in which the Aspergillus infection is limited predominantly to the tracheobronchial tree. It occurs primarily in severely immunocompromised patients such as lung transplant recipients. Here, we report a case of Aspergillus tracheobronchitis in a 42-year-old man with diabetes mellitus, who presented with intractable cough, lack of expectoration of sputum, and chest discomfort. The patient did not respond to conventional treatment with antibiotics and antitussive agents, and he underwent bronchoscopy that showed multiple, discrete, gelatinous whitish plaques mainly involving the trachea and the left bronchus. On the basis of the bronchoscopic and microbiologic findings, we made the diagnosis of Aspergillus tracheobronchitis and initiated antifungal therapy. He showed gradual improvement in his symptoms and continued taking oral itraconazole for 6 months. Physicians should consider Aspergillus tracheobronchitis as a probable diagnosis in immunocompromised patients presenting with atypical respiratory symptoms and should try to establish a prompt diagnosis.
CASE REPORT : Aspergillus Tracheobronchitis in a Mild Immunocompromised Host
( Byung Ha Cho ),( Young Min Oh ),( Eun Seok Kang ),( Yong Joo Hong ),( Hye Won Jeong ),( Ok Jun Lee ),( You Jin Chang ),( Kang Hyeon Choe ),( Ki Man Lee ),( Jin Young An ) 대한결핵 및 호흡기학회 2014 Tuberculosis and Respiratory Diseases Vol.77 No.5
Aspergillus tracheobronchitis is a form of invasive pulmonary aspergillosis in which the Aspergillus infection is limited predominantly to the tracheobronchial tree. It occurs primarily in severely immunocompromised patients such as lung transplant recipients. Here, we report a case of Aspergillus tracheobronchitis in a 42-year-old man with diabetes mellitus, who presented with intractable cough, lack of expectoration of sputum, and chest discomfort. The patient did not respond to conventional treatment with antibiotics and antitussive agents, and he underwent bronchoscopy that showed multiple, discrete, gelatinous whitish plaques mainly involving the trachea and the left bronchus. On the basis of the bronchoscopic and microbiologic findings, we made the diagnosis of Aspergillus tracheobronchitis and initiated antifungal therapy. He showed gradual improvement in his symptoms and continued taking oral itraconazole for 6 months. Physicians should consider Aspergillus tracheobronchitis as a probable diagnosis in immunocompromised patients presenting with atypical respiratory symptoms and should try to establish a prompt diagnosis.
한반도 상공의 ADEOS/IMG 관측 자료와 존데 자료의 비교 분석
조하만 ( Ha Man Cho ),김주공 ( Ju Kong Kim ),오성남 ( Sung Nam Oh ) 大韓遠隔探査學會 1999 大韓遠隔探査學會誌 Vol.15 No.3
ADEOS/IMG로 관측한 온도, 수증기, 오존의 분포를 1997년 (a)1월 10일, (b)1월 28일, (c)4월 2일, (d)6월 19일에 대하여 오산과 포항의 존데 관측 자료와 비교하였다. 전반적으로 오산의 자료와 비교한 (b)와 (d)의 경우는 대체로 존데 자료에 근접하였지만 포항의 자료와 비교한 (a)와 (c)의 경우는 상당한 차이를 보였다. 예를 들면 (b)와 (d)의 경우에 온도에 대한 RMSE와 편의 분석 결과는 700~300 hPa에서는 약 1~4 K, 그 이상의 고도에서는 4~5 K이상의 편의를 나타내는데, 이것은 존데에 의한 관측 자료의 신뢰성 수준에 대한 논의도 함께 이루어져야 할 것으로 사료된다. 오존 분포에 대하여 동일한 분석을 시행한 레벨 2 5_6_4_4 버전의 결과는 50~60 hPa 이상의 상층을 제외하고는 그 차이가 0.3 ppmv 이하로 나타났다. 1997년 6월 19일에 한반도 상공의 5개 지점에서 관측한 IMG 자료 중 레벨 2 5_6_4_4 버전의 결과는 온도, H2O, O3, CO의 연직 분포와는 달리 CO2, N2O, CH4, HNO3의 연적 분포는 관측 지점이 달라짐에 따라 최대 농도가 나타나는 고도를 중심으로 그 차이가 크게 나타났다. ADEOS(Advanced Earth Observing Satellite)/IMG(Interferometric Monitor for Greenhouse Gases) measurements-temperature, water vapor(H2O), ozone(O3) have been compared with the radio sonde and ozone sonde observations at Osan and Pohang stations for the 4 cases on 10 Jan.(a), 28 Jan.(b), 2 Apr.(c), and 19 Jun.(d) 1997 to detect the error ranges of the IMG data. It showed that the IMG data of the cases (b), (d) when the ADEOS passed over the central part of Korea were quite stable with the good agreement with the sonde observations, however, that of (a), (c) when the ADEOS passed over south-east coastal area were unstable with the larger differences from the sonde-observations. The RMSE and bias analyses of temperature for the stable cases (b), (d) showed that the differences between the IMG data and the sonde observations were about 1~4 K at the 700~300 hPa level and about 4~5 K or more at the higher level, and the IMG measurements tended to be larger than the sonde observations at the higher level above 200 hPa, while no typical bias was seen at the lower level. The RMSE and bias analysis for the version of level 2 5_6_4_4 of ozone showed that the RMSE of ozone were quite small, in general, except at the higher level above 50~60 hPa in the all 4 cases, however the bias was generally big with the positive value in the troposphere and the negative in the stratosphere. An example of vertical profile of trace gases such as CO2, N2O, CH4, HNO3, CO measured by IMG was also presented and it showed that the IMG data had large differences between the 5 different observation points.