자궁경부 상피내 병변 환자에서 LEETZ 원추절제술 후 HPV DNA Test 추적 검사의 임상적 의의

김미향,이태화,오영림,이천준,김원규 고신대학교 의과대학 2009 고신대학교 의과대학 학술지 Vol.24 No.2

Objective: This study was performed to investigate the clinical significance and timing of HPV test for follow up of patients after LEETZ treatment in CIN lesion Methods: From January 2005 to December 2008, 105 patients with CIN were treated with LLETZ at Kosin university gospel hospital, Busan, Korea. After LEETZ, HPV test and Pap smear were done after 3months. Every HPV typing was done by HPV DNA chip. Results: A total of 105 cases were enrolled in this study. There were 25 patients with CINⅠ, 38 with CINⅡ, 42 with CINⅢ. Mean age was 41.95 years (range, 23-71 years) at the time of LEETZ. Positive HPV DNA was found about 72.4% before LEETZ procedure and subtype 16 was the most common type (10.5%), follow by subtype 18 (6.6%) and subtype 58 (6.6%). During follow up after LEETZ, 65.6% had negative result after 3 month, 72.1% after 6 month, 73.9% after 9 month, 77.1% after 12 month, 79.2% after 15 month, 79.6% after 18 month, and 80.4% after 24 month. There were 2 cases of abnormal pap smear among persistent positive HPV DNA cases and no abnormal pap smear results in negative HPV DNA cases during follow up. Conclusion: Positive HPV DNA was found about 72.4% before LEETZ procedure and subtype 16 was the most common type (10.5%), follow by subtype 18 (6.6%) and subtype 58 (6.6%). We suggest that HPV DNA test is recommended per 6 Months, and close observation is recommended in persistent positive HPV DNA test cases.

정공저지층 BCP를 포함한 Organic light-emitting devices

하미영,문대규 순천향대학교 산업기술연구소 2007 순천향 산업기술연구소논문집 Vol.13 No.2

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생화학적 표식자를 이용한 골다공증의 평가

이규한,박영재,김미정,이상건,이강목 大韓再活醫學會 1998 Annals of Rehabilitation Medicine Vol.22 No.3

유아에 대한 부와 모의 언어통제유형 비교

임규란,이미숙 가톨릭대학교 생활과학연구소 2001 생활과학연구논집 Vol.21 No.1

The purpose of this study is to compare the verbal control modes used by fathers or mothers toward their children. The subjects of this study are 148 fathers and 159 mothers whose children attend kindergartens. For data analysis, t-test, one-way ANOVA and Scheffe's test, Pearson's correlation, and two-way ANOVA were used. The major findings are as follows: First, the status-oriented verbal control mode was most frequently used by both fathers and mothers, while the order-oriented verbal control mode was used less frequently by parents. Second, both fathers and mothers used three verbal control modes more often toward boys than toward girls. Third, the comparison of verbal control modes between fathers and mothers revealed that mothers, rather than fathers, used three verbal control modes more frequently toward their children. Fourth, mothers with higher education, rather than fathers with higher education, used the status-oriented verbal control mode and personality-oriented verbal control mode more frequently toward their children.

난분해성 유기물질 제거를 위한 오존/촉매 공정의 비교에 관한 연구

이규환,이유미,이동석 강원대학교 산업기술연구소 2006 産業技術硏究 Vol.26 No.B

Ozone alone and catalytic ozone processes were introduced for treatment of humic acid, which is representative refractory organic compound. The treatment efficiencies of humic acid in each process were analyzed in pH variation, DOC removal, and UV_(254) decrease. Mn loaded CAC catalyst was prepared by loading potassium permanganate onto the granular activated carbon surface. BCM-GAC and BCM-Silicagel catalyst were prepared by BCM. UV_(254) decrease in all processes was comparatively high with efficiency over 87%. DOC removal in ozone/GAC process was the highest with 78%, and removal rated for other processes followed the order ozone/BCM-GAC(62%) > ozone/BCM-silica gel(45%) > ozone/silica gel(43%) > ozone/Mn Loaded GAC(42%) > ozone alone(37%).

기능평가를 통한 장애유아의 행동지원 실험연구 분석 : 1997~2006년 사이의 연구를 중심으로

이미애,한성희,이규옥 국립특수교육원 2007 특수교육연구 Vol.14 No.2

이 연구는 유아를 대상으로 기능평가를 통한 행동지원을 제공한 실험연구를 분석하여 현재의 전반적인 특징과 경향을 분석하고 문제행동 기능의 결과가 중재와 어떻게 연결되는지 살펴봄으로써 문제행동 중재를 위한 향후의 방향을 모색하고자 하였다. 이 연구의 분석 대상 논문은 주로 중다기초선 설계법을 사용하며 중재는 일반교실이나 가정 등의 자연스러운 환경에서 교사나 부모 등의 자연스러운 중재자에 의해서 이루어지고 있었으며 목표행동은 대상유아의 바람직한 행동보다는 부정적인 행동이 많았으며 중재자의 행동을 목표행동으로 본 연구도 5-6편이 있었다. 연구의 질적 수준에 대한 변인들 중 관찰자간 신뢰도를 제외하고는 중재충실도, 사회적 타당도, 유지, 일반화 등에 대한 파악이 부족한 것으로 나타났다. 기능평가는 주로 일반학급과 가정 등의 자연스러운 환경에서의 부모나 일반교사를 대상으로 한 인터뷰와 연구자나 가족, 교사 등에 의한 관칠 방법, 연구자에 의한 기능분석 방법이 많이 이용되었다. 기능평가 방법으로는 직접관찰과 인터뷰가 가장 많이 이용되었으며 평균 2~3가지 방법을 사용하고 있었으며 문제행동의 기능은 관심끌기가 가장 높았고 획득과 회피, 감각적 자극 순으로 나타났다. 행동의 기능에 따른 중재전략을 살펴보면 획득기능과 회피 기능을 가진 경우에는 선행사건 예방전략과 후속반응전략이 가장 많이 사용되었고 관심의 기능을 가진 경우에는 후속반응전략이, 감각적 자극을 목적으로 한 문제행동 중재에는 문제행동을 차단하는 전략이 사용되고 있었다. The purpose of this study was to analyze experimental studies that intervened young children's challenging behavior using functional assessment and to provide information and suggestions on future research. To accomplish this purpose, the elements of experimental study, the quality features of study(interobserver agreement, fidelity of intervention, social validity, maintenance and generalization of effect) and methods of functional assessment and intervention strategies were analyzed. The major findings of this study was followed. First, most of the analyzed articles used multi-baseline design, and the interventions were implemented were natural settings like classroom and home, and the interventionists were natural people like teachers and parents. Target behaviors were focused on problematic behaviors of subjects than desirable behaviors. Second, among the 5 quality features of study I chose, fidelity of intervention, social validity, maintenance and generalization of effect were identified poorly except interobserver agreement. Third, functional assessment was implemented by natural people like teachers and family members, and at the natural settings like classrooms and home routine. Interview to teachers and family members and direct observation by researchers, teachers and family members were used. The most common functional assessment methods were direct observation and interview. And 2 or 3 methods were used one study. The order of the function of problematic behavior was attention, obtain tangible, escape, and sensory stimulation. As an interventions strategies, antecedents events, alternative skills and consequent responding were applied comparatively the same rate. Based on this results, some recommendations are suggested.

대장균에서 대량 생산한 B형 간염바이러스 X 단백질의 발현 특성

정미경,민성식,이희구,오은숙,이혜경,이태규,임종순,박순희 大韓免疫學會 1996 大韓免疫學會誌 Vol.18 No.4

The X protein of human hepatitis B virus (HBx) consists of 154 amino acids and transactivates various cellular and viral promoters and enhancers. This protein has been also implicated in virus-mediated oncogenesis of hepatocytes. However, there are still controversial reports on its mechanism related to its oncogenicity. To elucidate the presence of any correlation between immune responses against HBx protein and hepatitis pathogenicity, convenient sources for recombinant HBx protein and anti-HBx antibodies are necessary. Three recombinant vectors were constructed to express the X open reading frame (ORF) of hepatitis B virus in E. coli. Recombinant E. coli transformed with one of these plasmid, pET- AX plasmid, which containd X ORF deleted in N-terminus, did not produce HBx protein. However, when X ORF containing 154 amino acids was cloned in pET, the HBx protein was produced. A GST fusion protein containing 146 amino acids encoded by ORF X was also successfully expressed. This protein was produced in large quantities of more than-60% of total proteins However, nonfused HBx protein was produced in small quantities. As both proteins were produced in inclusion bodies, HBx recombinant GST-fusion protein was further proccessed for purification. GST-X fusion protein was solubilized by sarkosyl. The solublized protein was purified by affinity to glutathione sepharose bead. The purified HBx protein was fairly good immunogen eliciting good humoral immune responses in mice. We produced polyclonal antibodies against HBx recombinant proteins and confirmed the specificity of the antibodies.

청옥산(경북)의 습지 및 인근지역의 식물상

정규영,김미숙 안동대학교 농업과학기술연구소 2000 農業科學技術 硏究論文集 Vol.7 No.-

본 연구는 경북 청옥산(해발 1276.5m, 북위 37˚00'- 37˚05', 동경 128˚55'- 129˚00')의 남사면의 습지와 그 일대의 관속식물의 분포 및 보존대책 수립을 위한 기초조사로 수행되었다. 1998년 9월부터 1999년 8월까지 4회에 걸쳐 채집된 관속식물의 총수는 89과 268속 369종 1아종 57변종 7품종의 434종류이었으며, 이중 습지에 존재하는 관속식물은 총 67종류이었고, 습지형식물은 18종류이었다. 본 지역에 분포하는 한국특산 식물은 12종류이었으며, 식물구계학적 특정식물종은 55종류이었고, 본 조사에서 처음으로 남한에 분포가 확인 종류는 Lycopus uniflorus Michx.(털쉽사리) 1종류이었으며, 귀화식물은 9종류이었다. 본 지역의 습지는 인위적인 요인에 의해 심하게 훼손되어 본래의 상태로 회복시키는 것은 불가능하라라 여겨지며, 지금의 상태를 유지하기 위해서는 인위적으로 식재된 수목의 제거와 토사의 유입 방지, 유속을 더디게 하는 수로의 개선으로 습지환경을 형성시켜 주는 것이 필요할 것으로 판단되었다. This study was carried out to clarify the distribution of vascular plants of wet land and it's around area about south slope of Mt. Ch'ong-ok (1276.5m a.s.l., 37˚00'∼ 37˚05'N, 128˚55'∼ 129˚00'E), Kyungpook. The vascular plants, collected from September 1998 to August 1999 four times, consist of total 434 taxa; 89 families, 268 genera, 369 species, one subspecies, 57 varieties and seven forms. Among them, plants distributed in wet land were 67 taxa, hygrophytes 18 taxa. Korean endemic plants of this area were 12 taxa, and the special plant species based on floral region by Ministry of Environment(1999) were 55 taxa, new distrbutional plants of South Korea were one speceis, Lycopus uniflorus Michx, and the naturalized plants were nine taxa. The wet land in this area were destroyed seriously by artifical factors, therefore, may be impossible to restore original conditions, but the methods of being maintained present state is thought to be removed artifical vegetation, prevented to inflow of soils from around area and reconstructed waterway for delaying speed of runing waters and forming wet land environments.

생체내 심근허혈-재관류 모델에서 멜라토닌의 재관류 부정맥에 대한 심근보호작용에 관한 연구

조규종,김원,홍정석,이미우,장성은,오세현,임경수 대한응급의학회 2001 대한응급의학회지 Vol.12 No.4

Background: Recently, a few studies demonstrated that melatonin reduced the severity of myocardial reperfusion injuries, such as reperfusion arrhythmias. However, it is uncertain whether the melatonin reduces reperfusion arrhythmias in thus anesthetized animals because results were obtained using isolated hearts. Thus, to see whether melatonin reduces reperfusion arrhythmias in anesthetized animals, we examined the effect of melatonin on the incidence of reperfusion arrhythmias in an anesthetized-cat model of regional ischemia. Method: Adult mongrel male cats(n=30, 2.9∼4.2 kg) were anesthetized under positive-pressure artificial ventilation with room air. The animals of the control group(n=15) were subjected to 20-minute left anterior descending coronary artery(LAD) occlusion followed by abrupt reperfusion. The animals in experimental group(n=15) were divided into two. Group I(n=6) was pretreated with melatonin, 1 mg/kg, before occluding the LAD. Group II(n=9) was pretreated with melatonin, 10 mg/kg. The animals in experimental group were subjected to ischemia/reperfusion insult following drug treatment: melatonin was applied intra-peritoneally for 3 minutes just before LAD coronary artery ligation. The Fisher's exact test was used to compare the data from different animal groups. p<0.05 was considered significant. Results: The incidence of ventricular fibrillation(VF) during the reperfusion phase in group II(pretreated with melatonin, 10 mg) was significantly smaller than that in the control group(p-value = 0.0029). However, no statistically significant difference of VF incidence was found between group I(pretreated with melatonin, 1 mg) and the control group. Conclusion: Employing an anesthetized-cat model of regional cardiac ischemia, we investigated the dose-dependant effects of melatonin on reperfusion-induced arrhythmia. The cats pretreated with 10 mg/kg of melatonin before ischemia had a significantly reduced incidence of lethal reperfusion-induced arrhythmia, but there was no difference between the cats pretreated with 1 mg/kg of melatonin before ischemia and the control group.

정수처리 공정에서 철과 망간 잔류 농도에 관한 연구

최미리,서규태,문병현,이택순 7개 국립대학교 환경연구 논문집 공동발행 위원회 2003 공업기술연구 Vol.3 No.-

This study was to investigate the cause of the color and turbidity in a small-scale water treatment plant using lakes water as water supply source. Water qualities in aqueducts, in depth of the lakes and in each process were measured. And effects of prechlorination on each unit operation in the water treatment, especially on iron and manganese removal, were studied. The concentration of iron and manganese was 0.13, 0.08 mg/L in the surface water and 1.93 and 0.433 mg/L in 15 m depth of the lake. Higher iron concentration of the inlet was measured to 5.35 mg/L which was caused by the steel aqueduct corrosion. The optimun coagulation dosages of Poly Aluminum Chloride(PAC) and Liquid Aluminum Sulfate (LAS) were 17.5 mg/L and 35 mg/L. Pretreatmnet of raw water with CaCOl improved the removal efficiencies of iron and manganes. However, more efficient method for removing iron and manganes was required.