수용성 키토산 유도체를 이용한 방사성 스트론튬의 시험관내 흡착능 및 마우스내 체외배출 효과

김희경,송해미,조덕제,조만기,이정석,이응호 동서대학교 부설 연구소 1996 연구소 논문집 Vol.1 No.-

키토산은 천연착화제로서 효용가치가 높은 무독성의 천연고분자로서 키틴으로부터 탈아세틸화시킨 유도체이다. 키토산에 인산기을 도입한 Water-solubel phosphorylated Chitosam(WSPC)의 Sr에 대한 시험관내 흡착률은 pH 3에서는 97.8%이었으나, pH 5와 pH 7에서는 모두 100%이었다. WSPC를 농도별(0.01%, 0.1%, 1%)로 희석하여 11주령의 ICR 수컷 마우스의 위내로 투여한 직후 Sr을 같은 경로로 처리한 다음, WSPC의 Sr에 대한 체외 배출효과와 골내 잔존률을 평가하였다. 또한 Sr와 농도별 WSPC 처리후 6일째에 척추, 두개골, 대퇴골, 경골, 치아 및 꼬리뼈 등에 대해 Sr 잔존율을 측정하였다. 0.01-0.1% 사이의 WSPC를 접종한 마우스 투여군이 Sr만 경구 투요한 대조군에 비하여 골조직에 잔존하는 Sr의 침착률이 낮게 나타났다.(P<0.01). 또한 방사성 스트론튬의 분뇨를 �蔥�배출 효과 역시 WSPC 처리군이 대조군에 비해 유의성이 인정되었고(P<0.05), 대체로 WSPC 농도가 높을수록 그 효과는 비례하였다.(P<0.05). 본 실험에 사용된 WSPC는 체내에 유입된 Sr을 제거하는데 효과가 인정되어 방사성 스트론튬의 방어약제중 하나로 활용할 수 있다고 판단되었다.

대장암환자에서 자연살해세포의 변화

김광호,심강섭,박응범,강은숙,홍기숙 대한대장항문학회 1998 Annals of Coloproctolgy Vol.14 No.3

대장암조직에서 아포토시스 검출 방법에 관한 연구

김광호,심강섭,김성숙,구혜수,박응범 梨花女子大學校 醫科大學 醫科學硏究所 1997 EMJ (Ewha medical journal) Vol.20 No.2

1989년 1월부터 1992년 12월 사이에 대장암으로 근치적 절제술을 시행받은후 추적조사가 가능한 45명의 환자를 대상 대장암조직의 Hematoxylin-Eosin염색을 통하여 apoptotic body를 광학 현미경하에서 400배율 하에서 관찰하여 암세포 100개당 평균개수를 세었으며 TUNEL 방법을 이용하여 면화학적염색을 통하여 염색정도를 판정을하여 대장암에서 아포토시스의 정도를 판정하여 다음과 같은 결과를 얻었다. Dukes병기 A 일 때 apoptotic body가 평균 1.93개, B 일 때 2.66개, C 인 경우 2.76개 였으며 분화도에 따라서는 고분화암인 경우 2.51개, 중등도분화암인 경우는 3.01개, 저분화암인 경우는 2.15개였다. 5년내 사망한 환자에서는 3.55개 였으며 5년이상 생존한 환자에서는 2.8개였다. 형태학적으로 apoptotic body의 수를 세어 아포토시스를 측정하여 예후예측인자로 사용할 수 있으리라 사료되며 아포토시스와 p53의 발현과의 관계를 보았을 때 특별한 상관관계를 발견 할 수 없어 bcl-2의 발현과 더불어 아토토시스와의 관게에 대하여 좀더 연구가 되어야 하겠다. Objectives : Apoptosis is a specific mode of cell death recognized by a characteristic pattern of morphological, biochemical, and molecular changes, There are several methods of detection of apoptosis. Morphological changes involve a characteristic pattern of chromation and cytoplasm. The landmark of apoptosis is endonucleolysis, with nuclear DNA initially degraded at the linker sections to fragments equivalent to single and multiple nucleosomes. Detection of DNA fragments is situ using the terminal deoxyribonucleotidyl transferase(TDT)-mediated dUTP-digoxigenin nick end labeling (TUNEL) assay is increasingly applied to investigate apoptosis. We studied the detection method of apoptosis morphologically and by using TUNEL assay and examined the correlation of p53 expression and apoptosis. Methods : Forty-five cases of colorectal cancer were selected. The number of apoptotic bodies was expressed as a number per 100 cancer cells. The TUNEL assay was performed with in situ Apoptag kit®. Results : The mean number of the apoptotic bodies was 2.28 in the patients who survived over 5 years after curative resection and 3.55 in the patients who died within 5 years(p=0.001). There was a relationship between the number of apoptotic bodies which were measured by morphologic study and the results which were measured by TUNEL assay. There was no relationship between p53 expression and apoptosis. Conclusion : These results suggest that the frequency of apoptotic bodies may be a prognostic factor for colorectal cancer and apoptosis could be measured by morphological study without special study.

초음파 어레이를 이용한 이동 로봇의 장애물 회피에 관한 연구

김병남,권오상,김기호,이응혁 建陽大學校 1999 建陽論叢 Vol.- No.7

For mobile robot, the navigation effectiveness can be improved by providing autonomy, but this autonomy requires the mobile robot to detect unknown obstacles and avoid collisions while moving it toward the target. This paper presents an effective method for autonomous navigation of the mobile robot in structured environments. This method uses ultrasonic sensor array to detect obstacles and utilizes force relationship between the obstacles and target for avoiding collisions. Accuracy of sensory data produced by ultrasonic sensors is improved by employing error eliminating rapid ultrasonic firing (EERUF) technique. Navigation algorithm controlling both the velocity and steering simultaneously is developed, implemented to the mobile robot and tested on the floor filled with the cluttered obstacles. It is verified that from the results of the field tests the mobile robot can move at a maximum speed of 0.66 m/sec without any collisions.

MOCVD법에 의해 제조된 Fe₂O₃-TiO₂계 박막의 내식성과 광전류 응답 특성에 관한 연구

金顯洙,尹在弘,金明鎬,杉本克久,邊鷹善 國立 昌原大學校 産業技術硏究所 1998 産技硏論文集 Vol.12 No.-

Fe₂O₃-TiO₂ films were formed by MOCVD technique, and their corrosion resistance and photoelectrochemical property were examined in acidic, neutral and alkaline solutions. The corrosion resistance was examined by an anodic polarization test, and the photocurrent response by a photoelectrochemical polarization test. The photocurrent and quantum yield maximum of the films depend on the titanium cationic fraction(Xη) in the films. They decreased in the range of Xη=0.0-0.70, then increased in the range of Xη=0.70-0.95, and finally decreased in the range of Xη=0.91-1.0 with increasing Xηvalue. The increase in the photocurrent and quuantum yield maximum in the range of Xη=0.70-0.95 was attributed to the amorphous structure. Such a change in the photocurrent and quantum yield maximum was independent to the solution pH. The corrosion resistance of the films increased with increasing Xηvalue of the film in acidic, neutral and alkaline solutions. Therefore, the films with Xη=0370-0.95 can be concluded to have high photocurrent response and high corrosion resistance.

回轉圓板法 下水處理 微生物의 再增殖現象

金應鎬 弘益大學校 1987 弘大論叢 Vol.19 No.2

A new growth model, named 'Secondary Growth Model(SGM)',has been developed for microorganisms' growth in mixed-culture system. SGM has proved to be reasonable for an explanation an RBC microorganism growth through batch experiments conducted without feeding primary substrate. Summary of this study is as follows: (1) Microorganisms themselves in a biological reactor generate a new soluble material named `secondary substrate' and its generation results in decrease in sludge mass. (2) Secondary substrate is believed to have biological degradability. (3) In case of high level treatment, consumption of secondary substrate becomes very important factor in choosing a treatment process. (4) RBC biofilm having stable microbiological ecosystem is considered to consume secondary substrate as well as primary substrate.

藥用Sulfonamide 類의 Schiff 監基의 合成과 抗菌作用

金鍾潤,張億奎,田應鎬 嶺南大學校府設 基礎科學硏究所 1983 基礎科學硏究 Vol.3 No.-

Twelve new Schiff bases were synthesized by condensation of two kinds of medicinal sulfonamides with eight kinds of aromatic aldehydes. They were identified by means of the elemental analysis, and UV and IR spectrochemical analyses. Antibacterial activity of the Schiff bases was tested in vitro against one gram-positive and seven gramnegative bacterial species. The results show that some of the compounds are more effcetive than each of corresponding sulfonamides against gram-positive St. aureus, exerting more remarkable activity compared to that against the gram-negative species. Therefore, the mechanism of action of the Schiff bases seems to be different from that of medicinal sulfonamides.

택지개발지구내 도시공원의 분포특성에 관한 연구

김수봉,정응호,김정환,류지원 한국정원학회 2002 한국전통조경학회지 Vol.20 No.2

To generate a suggestion for a better framework especially the distribution and locations of urban parks in residential areas, ‘residential site development districts’ in Daegu city were tested based on the community involvement and user’s demand for visting. Service-range, advantage and disadvantage in use derived from the locations of the parks in the districts were analyzed according to the concept of density,character assessment, structure in the distribution and locations. Suggestions to give residents more friendly surroundings with ’easy access’ and ’friendly characteristics of each parks’ are as follows; ⅰ) Although most of the tested sites are satisfying the legal criterion on the required level of size of urban parks in each district, there are big difficulties to have rich natural environment in the case of extensive size of residential sites due to too low applied-criterion(0.6㎡ per person) which is a minimum requirement. ⅱ) Although the rate of size of parks in the sites were gradually improved recently, the portion for each person had not improved at all. Therefore, it is needed that new detailed criteria in different level rooted in the character of individual sites such as population and characteristics of parks. ⅲ) Although the distance of parks is within the range of use, the locations are on the edge of sites and residential districts where is not on the everyday route. Therefore to give more easy accessibility, the urban parks in the residential sites should be located on the daily life route where is a more common area in future plans.

한약에 의해 유발된 급성 간질성 신염 2례

김덕윤,박동건,김응석,강영모,양창헌,이정호,이동철,이영현,김정란 동국대학교 경주대학 1996 東國論集 Vol.15 No.-

간질성 신염은 신장의 간질을 선택적으로 침범하는 염증성 질환으로 여러가지 원인에 의해 유발되며, 이 중 약물에 의한 경우는 각종 항생제, 비스테로이드성 진통제, 항경련제, 이뇨제, 면역억제제등에 의한 증례들이 보고되고 있다. 현재 각종 질환-특히 만성질환-의 치료에서 한약이 차지하는 비중이 적지 않으나, 그 각각의 성분들이 유발할 수 있는 부작용들에 대한 연구는 거의 없는 실정이다. 저자들은 관절염 치료를 위해 중국산 한약을 복용한 후 복통, 피로감 및 육안적 혈뇨를 주소로 내원한 두 환자에서, 단백뇨와 신기능 장애를 보여 시행한 신조직 생검상 급성 간질성 신염에 합당한 병리학적 소견을 보이고, 한약 복용 중지 후 급속한 회복을 보여, 한약에 의해 유발된 것으로 사료되는 급성 간질성 신염 2예를 경험하였기에 문헌고찰과 함께 보고하는 바이다. Acute interstitial nephritis is a disease characterized by renal interstitial inflammatory cell infiltration and acute renal functional deterioration. This is caused mainly by antibiotics, NSAID and diuretics such as thiazide, but cases induced by herb medication are rare. We experienced two cases of acute interstitial nephritis after treatment with herb medication. One 71-year-old female patient and the other 60-year-old female were admitted to the hospital because of general weakness and gross hematuria. Microscopic hematuria, pyuria, and proteinuria were presented. After definitive diagnosis with a renal biopsy, we noted rapid recovery of renal function by drug withdrawal and steroid therapy. We report these cases with a review of the referenced literatures. Key Words : Herb medication, Acute Interstitial nephritis.

계층분석기법을 이용한 하수관거 결합항목별 가중치 산정

김응석,이정호,김중훈 대한상하수도학회 2003 상하수도학회지 Vol.17 No.2

In domestic sewer rehabilitation works, the numerical weighting based on number of sewer defect is used to determine the propriety of rehabilitation order. In case of the decision standard in each defect contents of inside and outside of the country, the weight for determining sewer rehabilitation order is composed of several distribution of marks, not considering the weight in each sewer defects. In this study, the fifteen defect contents are determined suitable for domestic situation based on the sewer defect data of inside and outside of the country. Also, we select persons in charge of the business to calculate the detail weight and pose questions about important level of each sewer defect contents. The results of using Delphi method which is a question survey method apply the AHP and calculate the weight in each sewer defect. The appropriate marks matrix of sixteen sewer defects is made for laying precise decision standard of sewer condition through the result of analysis. The marks matrix of sixteen sewer defects can solve the complicated decision making problems of sewer rehabilitation works.