다핵균 및 단핵균으로부터 고protease 생산균주의 Screening

송상호,임현수,최명락,유은정,김성영 여수대학교 1998 論文集 Vol.13 No.2

4 multinucleate and 7 mononuclear fungi were investigated for screening of high protease productive strains. The protease activity of mononuclear strains was generally greater than that of multinucleate strains. For acid proteinase, Aspergillus awamori AOK 1007 and Asp. oryzae shoyu 2 as mononuclear strains were selected in glucose-polypeptnoe medium. For acid carboxypeptidase, two mononuclear strains were screened as Asp. awamori AOK 1007 and Asp. usamii mut. shirousami AOK 1011 in glusose-sodium glutanate medium. In this result, mononuclear fungi appeared higher protease production than that of multinuclear fungi. So, mononuclear fungi may gave many merits to improve protease activity by using of mutation or cell fusion technology.

과산화효소를 이용한 백혈구 측정용 뇨 검사지 제조에 관한 연구

송은영,변시명,김희정,김종완,정태화,이홍수,최인성 THE KOREAN SOCIETY FOR BIOMEDICAL LABORATORY SCIEN 1996 Journal of biomedical laboratory sciences Vol.2 No.2

백혈구에 존재하는 과산화 효소를 이용하여 뇨 중 백혈구의 수를 간접적으로 측정하는 새로운 방법의 뇨 검사지를 개발하였다. 과산화 수소원으로서 포도당 산화효소와 포도당을, 색원체로서 tetramethylbenzidine을 처리하여 백혈구 측정용 뇨 검사지를 제조하였으며 검출한계는 10cells/㎕ (5 cells/hpf)이었다. 개발된 백혈구 측정용 뇨 검사지로 뇨를 분석하였을 때 뇨 1/㎕당 10-25cells의 백혈구가 존재할 경우에는 2분내에 연녹색을, 75-250 cells/㎕에서는 녹색을 보였고 500 cells/㎕ 이상에서는 녹청색을 보였다. 172명의 환자뇨를 대상으로 뇨중 백혈구 수치를 현미경 측정 결과와 본 연구에서 개발한 백혈구 측정용 뇨 검사지와 현재 수입 시판 중인 미국의 A사 제품과 B사 제품으로 분석 비교한 결과 좋은 상관관계를 보였다. 이와 같은 평가분석을 통하여 볼 때 본 연구에서 개발한 백혈구 측정용 뇨 검사지는 에스테라아제를 이용한 백혈구 측정용 뇨 검사지와 함께 뇨중 백혈구를 스크리닝 하는데 효과적임을 알 수 있었다. A new test strip to detect leukocytes using the myeloperoxidase in urine was developed. The reagent strip contains tetramethylbenzine, glucose and glucose oxidase. The detection limit was between 10 cells per 1㎕ urine(5 cells/hpf), showing greenish yellow color in the range of 10-25 cells/㎕, green color in the range of 75-250 cells/㎕, greenish blue color in the range of 500 cells/㎕. The result can be obtained within two minute. The performance of the new method was evaluated by comparing the results of microscopic examination and other commercial products. Good correlations were shown between the values obtained by our urine strip and those by other commercial products with 172 urine samples. The results were proven that new methods were useful as primary screening reagents to detect leukocytes in urine.

수정진동자를 이용한 2가이온의 전기화학적 특성 분석

송성훈,김종민,박지선,손진언,김영한,장상목 동아대학교 공과대학부설 생산기술연구소 1998 生産技術硏究所硏究論文集 Vol.3 No.2

This paper shows eletrochemical extraction property changes of dyed cations by the various eletrochemical conditions using quartz crystal technique. The quantitative and qualitative changes of electrode surface was monitored measuring oscillation parameters of quartz crystal. The results suggest that the eletrochemical extraction of cation is influenced by the various factors such as ionic size, ionic intensity and tendency of ionization.

近代 Olympic 大會의 Nationalism化 傾向에 關한 硏究 : 政治的인 面을 中心으로 Tqcused on the Politics

宋銀燮,吳大成 又石大學校 1987 論文集 Vol.9 No.-

Current olympic games became to atlract people's concern world widely such development owed to the present political environments. But, under such development, political tenseness is involved particalary by nationalism. For example, the pure idea of olympicgames is contaminated by each nation's hot congestion, for another political benefit. So some solutions to this situation can be demonstrated like followings. 1. Even thoogh impossible, It is ideal to establish the etenal place for olympiad to remove each countriy's power games. But, there are a few problems such as economic difficulty. Some methodologies can be operated to compensate for the dificulty if and only if some would agree with me. in the case it is impossible to realize, another way such as the olympiad can be held in neutralization countries by rotation. 2. I think it is ideal to open olympiad without the showing of each country's national flag and instead of present domitary method for each country, that is to say, even though it is impossible to. By the idea of olympic, it is deserable not to play national songe and not to contribute medals for winners for the prevention of nationalism. And I think it is ideal to give something else ofr the celebrat of the hory spirit of winning.

쇠뼈를 이용한 산미스트의 흡착 및 제거

송민종,서성은,전의찬 동신대학교 환경연구소 1997 환경연구 Vol.2 No.1

금속표면처리시설과 축전지 제조시설 둥에서 발생하는 산미스트를 효과적으로 제거하기 위하여 질산미스트 및 황산미스트를 포함하고 있는 오염된 공기를 가정과 대중음식점 등에서 폐기물로 발생되는 소의 뼈(이하 '쇠뼈')를 이용하여 흡착제거함으로써 쇠뼈의 흡착제로서의 타당성을 평가하였다. 음식점에서 폐기물로 발생하는 쇠뼈를 탈회한 후 분쇄하여 황산미스트와 질산미스트를 흡착시켜 제거하고자, 흡착제의 크기와 흡착제의 량을 달리하면서 흡착 실험한 결과 흡착제의 크기를 작게 할수록, 흡착제의 량을 많이 할수록, 질산미스트 및 황산미스트를 흡수시킨 흡수액의 pH는 증가하였으며, 질산미스트 및 황산미스트의 농도는 감소하여 각각 97.29%와 96.09%의 제거율을 나타내었다. 쇠뼈를 이용한 질산미스트와 황산미스트의 흡착제거시 제거율이 높은 것은 쇠뼈의 성분에 가장 많이 함유되어 있는 칼슘화합물과 산미스트와의 산염기 반응에 의한 것으로 분석되었으며. 이에 따라 식당이나 가정에서 폐기물로 발생되는 쇠뼈는 본 실험에서와 같이 탈회 및 파쇄과정을 거쳐 흡착능을 갖도록 하면 산미스트와 같은 특정한 오염물질을 선택적으로 제거하는 데 유용하게 사용될 수 있을 것으로 판단된다. This study was carried out to investigate the adsorptive efficiency of cow bone. And adsorbates were nitric acid-mist and sulfuric acid-mist. As particle size of cow bone was decreased, removal efficiency of nitric acid-mist increased from 10.72ppm(before treatment) to 0.29ppm(after treatment) and those of sulfuric acid-mist was increased from 222.04ppm(before treatment) to 8.67ppm(after treatment) These results suggested that cow bone is a effective adsorbent for treatment of acid-mist

연쇄알구균 감염에 의한 급성 인후염 후 반응성 관절염 1예

박은하,도연실,양정채,강미라,서현주,정숙인,손준성,기현균,오원섭,백경란,송재훈 대한감염학회 2004 감염과 화학요법 Vol.36 No.6

연쇄알구균에 의한 급성 인후염 후 발생한 반응성 관절염은 급성 류마티스열의 개정된 진단기준을 충족하지 못하면서, 지속적 혹은 재발성의 비이동성 관절염과 발열, 홍반성 결절, 일시적 간기능 이상 등을 보이면서 심장염과 무도증을 드물게 동반하는 질환이다. 따라서, 발열을 동반한 급성 관절염 환자에서 감별해야 할 질환 중의 하나이며, 심근염 예방을 위한 페니실린 요법의 적응증 및 기간에 대해서는 아직 이론이 많은 상황으로, 보다 광범위한 연구가 필요하다. 저자들은 연쇄알구균에 의한 급성 인후염 1주 후에 발생한 하지의 다발성 비이동성 관절염과 함께 결절홍반 및 일시적 간기능 이상을 보인 환자에서 PSRA로 진단하고 비스테로이드성 항염증제 및 스테로이드로 성공적인 치료를 하였기에 이를 문헌 고찰과 함께 보고하는 바이다. Poststreptococcal reactive arthritis (PSRA) is associated with recent streptococcal infections. However, PSRA is distinct from acute rheumatic fever by its clinical manifestations: non-migrating arthritis, erythema nodosum or erythema multiforme, and transient elevation of serum transaminases. We experienced a 33-year-old man who presented with fever, arthritis of both knees and ankles, and erythema nodosums on extensor surfaces of lower legs which developed 6 days after the onset of pharyngitis symptoms. Blood and urine cultures were negative. Throat culture was negative for group A β-hemolytic streptococcus. The ASO titers increased up to 2080 IU/mL in sequential monitoring. The result of bone scan was compatible to arthritis of both knees and ankles. There were no signs or symptom of carditis. He showed clinical improvement with anti-inflammatory drugs (naproxen 1,000 mg/day and prednisolone 7.5 mg/d). PSRA should be included in the differential diagnosis of patients presenting with arthritis combined with fever.

지속적인 운동으로서의 골프가 아마추어 골퍼의 스트레스에 미치는 효과

오대성,송은섭,나정란 圓光大學校附設 生活體育硏究所 2001 生活體育硏究 Vol.11 No.-

This survey for the purpose of finding out the effects of Golf as a continuative exercise on the mental stress has made the following conclusion with the research results having examined the effects by using the stress measuring paper to the subjects of the amateur Golfers consisted of 183 (Male 98 and Female 85) in Chonbuk province. 1. The effects on the total stress levels as a continuative exercise shows that the male subjects (105.96±14.38) is higher than the female (104.05±11.49). 2. In the following stress levels the Golf has the effect showing that the male (19.72) is higher than the female (19.54) in the mental factor, and the male (35.74) is higher than the female (34.82) in the emotional and the anxiety factors. And it shows statistical significance (p=0.79). 3. The effects of Golf as a continuative exercise on the total stress levels according to the subjects' career shows that the group (108.03) whose career is more than 83 months is higher than the group (101.68) whose career is more than 49-82 months. 4. The effects of Golf as a continuative exercise on the mental stress in the following levels shows that the group (20.31) whose career is more than 83 months is higher than the group (18.71) whose career is more than 49-82 months in the mental factor, and the group (28.30) who career is more than 83 months is also higher than the group (25.76) whose career is more than 49-82 months in the physical and mental health factor respectively.

Phage Display 기법을 이용한 B형 간염 바이러스 Polymerase의 Rnase H 활성을 억제하는 인간 단세포군 항체의개발

이성락,송은경,정영주,이영이,김익중,최인학,박세광 Official Journal of the Korean society for immunol 2004 Immune Network Vol.4 No.1

八味地黃湯 煎湯液의 投與가 마우스의 自然致死 細胞의 活性度 및 免疫反應에 미치는 影響

朱松竹,李彦政,吳昇煥 圓光大學校 韓醫學硏究所 1992 원광한의학 Vol.2 No.1

This study was done to know the effects of Palmijihwangtang(八味地黃湯) on the cytotoxic activity of NK cells, the phagocytic activity of macrophages, the antibody production of B cells, and delayed-type hypersensitivity reaction by T cells in the mouse. The results were as follows. 1. The administration of Palmijihwangtang enhanced the cytotoxic activity of NK cells against YAC-1 cells. 2. The administration on Palmijihwangtang increased the production of reactive oxygen intermediate(ROI) production from macrophages without any effect on phagocytic activity. 3. The administration of Palmijihwangtang decreased the delayed-type hypersensitivity against dinitrochlorobenzene without affecting T cell subtypes. 4. The administration of Palmijihwangtang increased the antibody production against sheep red blood cells. The above results suggest that Palmijihwangtang could be used for the prevention and treatment of the diseases caused by the abnormalties of the body's immune functions.

혐기·호기공정을 이용한 생물막법에서 유기물, 질소 및 인제거에 관한 연구

이성기,송영일,김현중,진성기,김은영 조선대학교 환경연구소 2001 環境硏究 Vol.17 No.1

Anoxic and Oxic process with contact media is used to remove an organic matter, nitrogen and phosphorus in sewage which derives from domestic wastewater. Experiments have been done with various conditions that concentration of oxygen can be 3mg/ℓ, 4∼5mg/ℓ and 5∼6mg/ℓ by controlling an retention time can be 10hr, 12hr and 14hr, return rate can be 0%, 100% and 200%. As the concentration of oxygen is 2∼3mg/ℓ. The rate of removal of BOD. COD. SS. T-N and T-P is 87%, 76%, 82%, 34% and 24%. As the concentration of oxygen is 5∼6mg/ℓ. The rate of removal of BOD, COD, SS, T-N and T-P is 92%, 84%, 88%, 45% and 33%. In the rate of removal of an organism, nitrogen and phosphorus thus It well be known that the rate of removal an organic matter, nitrogen and phosphorus will be increased as the concentration of oxygen becomes higher. As th retention time is increased, the rate of removal of BOD, COD, SS, T-N and T-P is gradually higher in the rate of removal of an organic matter, nitrogen and phosphorus. Because contact time and contact area will be increased, which generates active substrate transfer and metabolic react according to increment of retention time. As the return rate is increased. The rate of removal of BOD, COD and SS is likely to be increased. In the case of T-N. It is also increased by 28% at 0% of the return rate and by 60% at 200% of the return rate. In the case of T-P, however, It is deceased, as the return rate is increased by 42% at 0% of the return rate and by 30% at 00% of the return. As the return rate is increased the retention time is decreased, which causes less activity of bacterial which affects that the rate of removal of Phosphorus is decreased.