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한중일 의뢰행동에 나타나는 상호행위 양상 연구-대화개시부의 분석을 중심으로-
김종완 중앙대학교 일본연구소 2018 日本 硏究 Vol.0 No.48
The current study analyzes the aspects of inter actions taken by the requester and the requestee by observing Korean, Chinese, and Japanese college students who ask for a recommendation from their advising professors. The interactions expressed during the initial conversation are (1) greeting when entering the room, (2) request for self-introduction, (3) request to sit down, and (4) making the request. The current study makes the following observations about interactions of the Korean, Chinese, and Japanese students. (1) When the requester entered his or her advising professor’s office, Korean professors (requestees) only used response expressions without saying greeting expressions. (2) When requesting self-introduction to the requester, Japanese-speaking requestees utilized various levels of honorific speech. (3) When the requestee asked the requester to take a seat, Japanese- and Chinese-speaking requesters sat down as they used expressions of gratitude to the professor, but Korean-speaking requesters only said “yes.” (4) Korean-speaking requesters took the initiative in requesting the recommendation, while Chinese-speaking students indirectly approached the subject when their advising professor asked their purpose of visit. In addition, Japanese speakers tended to begin their request with expressions of apology. As shown, the current research reveals that Korean-, Chinese-, and Japanese-speaking requesters and requestees take different forms of interaction in short discourses made when beginning a conversation.
Isolation of Lysozyme from the Hemolymph of Sweet Potato Hornworm,Agrius convolvuli Larvae
김종완,여성문 한국곤충학회 2003 Entomological Research Vol.33 No.3
Lysozyme plays a central role in initiating and maintaining the antibacterial defense response of insect. A new family member of insect lysozyme, an antibacterial peptide, has been isolated from the hemolymph of the fifth instar larvae of Agrius convolvuli. Vaccination was proceeded with E. coli K12 D21 (4×106 cells of log phase) injection into the abdomen of the larvae. Agrius lysozyme was isolated by cation-exchange chromatography and RP-FPLC, and sequenced by HPLC system. It was observed that the purified Agrius lysozyme was heat-stable and had a molecular weight of approximately 15 kDa by SDS-PAGE. The N-terminal amino acid sequence of Agrius lysozyme is K-H-FS-R-C-G-L-V-Q-E-L-F-W-Q-G-F-P with the highest similarity to that of Heliothis virescence, and it has been identified that the Agrius lysozyme belongs to c type lysozyme.
Xanthomonas arboricora pv. pruni에 의한 복숭아 세균성가지마름병의 발생
김종완 한국식물병리학회 1999 식물병연구 Vol.5 No.2
A new bacterial disease was found on trees of peach(Prunus persica var. vulgaris Max) at Kumho Kyungbuk in April 1999. The disease usually occured on over wintered buds branchs and stems of the Cheon-Hong cultivar. The buds died without sprouting and the branches showed entire wilting Droplets of bacterial ooze was occasionally running down the surface of diseased plants under moist condition. Artificial needle prick inoculation with isolates obtained from branchs of naturally infected plants produced symptoms similar to those occuring under natural condition. On the basis of bacteriological characteristics and pathogenicity on the host plant of the organism the causal bacterium was identified as Xanthomonas arboricora pv. pruni and this disease was proposed to name "Bacterial branch blight of the peach tree"
스팸 대응 시스템에서 특징 추출 방법 비교에 관한 연구
김종완,김희재,강신재,황운호 한국지능시스템학회 2006 한국지능시스템학회 학술발표 논문집 Vol.16 No.2
본 논문에서는 스팸 대응 시스템의 특징 추출 방법들을 비교한다. 실험 결과는 퍼지추론 방법이 정보획득량, 카이제곱 통계량, 상호정보 방법에 비하여 정확률과 재현율의 결합 척도인 F-척도면에서 월등한 성능을 보여주지는 않는다. 하지만 제안된 퍼지추론 방법은 사용된 특징들의 수에 비례하여 성능이 증가하므로 좋은 특징 추출 방법으로 간주된다. 따라서 본 연구는 무수한 스팸 메일로 고통 받는 전자우편 사용자들을 위한 스팸 메일 필터링 시스템 개발에 도움을 줄 수 있다.
김종완,이영주,탁연빈 한국임상수의학회 2003 한국임상수의학회지 Vol.20 No.1
Little is known to data about the in vitro activity of antimicrobial agents aganist Brucella canis (B canis) isolated from Korea. Our study aimed at determining the in vitro activities of 15 antimicrobial agents against 3 isolates and 52 isolates of B cains from dogs in 1994 and 2002, respectively. In minimal inhibitory concentration (MIC) study, minocycline and doxycycline showed the lowest MICs (<0.06~0.5mg/ml). Gentamicin, streptomycin, ciprofloxacin, norfloxacin and rifampin showed MICs in the range of less than 1mg/ml. Lincomycin and sulfisoxazole showed the highest MICs (>32mg/ml). Interestingly, MICs of macrolides (erythromycin, spiramycin, tylosin) against 52 isolates in 2002 were 16~64 times higher than that of 3 isolates in 1994. In minimal bactericidal concentration (MBC) study, gentamicin, streptomycin, ciprofloxacin and norfloxacin showed the lowest MBCs [0.12~1mg/ml (1~2 times higher than MIC)], but minocycline and doxycycline showed the highest MBCs [8~32mg/ml (128 times higher than MIC)]. Rifampin showed the MBCs in the range from 2 to 4mg/ml (2~4 times higher than MIC).
김종완,황성규,이기창,이한섭,박종주 한국환경보건학회 1998 한국환경보건학회지 Vol.24 No.3
Chitin is known as biodegradable natural polymer. In spite of various application of chitin derivatives from waste marine sources, commercial use of chitin has been limited due to high resistance to chemicals and the absense of proper solvents. We chitin prepared through the decalcification, bleaching and deproteination from Protunus trituberculatus shells by change of Hackman's method. Also, Microcrystalline chitin made by hydrolysis that was reduce made of resistance solvents used by dilute hydrochloric acid, ultrasonic and hydrogen peroxide. Crosslinked chitin derivatives were preparaed from chitin with crosslink agents(epichlorohydrin, 1,3-dichloropropanol) follwed by crosslinkage at 6C position. The effects of these parameters on chitin dervatives were invastigated by IR, DSC, XRD, BET, PSA and SEM. SEM analysis showed that both chitin and crosslinked chitin had a particle shaped morphology.
Purification of Lysozyme from Hemolymph of Tobacco Cutworm, Spodoptera litura
김종완,여성문 한국곤충학회 2003 Entomological Research Vol.33 No.4
Lysozyme is one of the components of the innate immunity of the insects. The lysozyme has been isolated by heat treatment, cation exchange, and reversed-phase chromatography from immunized hemolymph of last instar larvae of Spodoptera litura. The hemolymph immunized with the insect nonpathogen, Escherichia coli was collected 2 days after the abdominal injection. The molecular weight of Spodoptera lysozyme was estimated to be about 15 kDa by SDS-PAGE and it is great similarity with Agrius lysozyme, which recently puri.ed from larval hemolymph of Agrius convolvuli.