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들깨 종자 활력 수준별 초저온 동결보존 후 발아율 및 Ascorbate Peroxidase 활성 변화
이영이,이명희,이정윤,이태윤,손은호,박홍재 한국환경농학회 2017 한국환경농학회지 Vol.36 No.4
식물 종자를 비롯한 각종 유전자원을 액체질소에 저장하면 유전형질 특성의 손실 없이 장기간 보존할 수 있다. 본 연구에서 보존조건이 까다로워 단명 종자로 분류되는 들깨 종자를초저온 동결저장 방법으로 보존할 수 있는지를 조사하였다. 수집한 들깨 품종별 종자의 초기 발아율은 40-95% 수준으로 다양하였는데, 수분함량을 3-8%로 조절한 종자를 액체질소에 처리하여도 발아율은 감소하지 않았다. 종자의 수분함량이 4-5%인 종자의 초저온 처리 후 발아율이 가장 높았으며, 초기 발아율이 낮은 품종에서는 초저온 처리에 의해 발아율이 증가하기도 했다. 인위 노화처리에 따라 종자의 발아율과ascorbate peroxidase 활성은 감소하였으며, 품종별로 종자의활력 저하 정도는 크게 달리 나타났다. 대조 처리 종자와 비교하였을 때, 초저온 처리과정에 발생할 수 있는 산화스트레스가 들깨 종자의 활력을 저해하지는 않을 것으로 추정되었다. 따라서 들깨 종자를 4-5% 수분함량으로 건조시켜 초저온동결 저장하면 활력 손실 없이 장기간 보존할 수 있을 것으로판단되며, 노화가 급속히 진행되는 품종의 경우에는 고활력자원을 확보하거나 휴면타파 처리 후 초저온 동결 보존하는것이 유리할 것으로 생각된다. BACKGROUND: Seed of perilla (Perilla frutescens var. japonica Hara) is short-lived in conventional storage conditions. For long-term conservation of plant species, cryopreservation is the method currently available. This study was performed to find out reliable methods for a long-term storage of seeds of perilla as a genetic resource. METHODS AND RESULTS: Using seeds of 9 perilla cultivars, the effects of desiccation, aging, andcryopreservation on seed germinability and ascorbate peroxidase activity in the seeds were investigated. Initial germinability of the seeds was various, and dry seeds of all cultivars survived cryopreservation without loss of viability. The highest germination was achieved at 4-5% moisture content, and stimulatory effect of cryogenic temperature on the seed germination was observed in some cultivars. Accelerated aging of perilla seeds led to reduction in germination and ascorbate peroxidase activity, and the susceptibility of seeds to aging was different among the tested cultivars. No significant difference in germination was observed for the aged seeds of control and liquid nitrogen exposed. CONCLUSION: The results of this study suggest that cryopreservation at 4-5% moisture content would be a suitablemethod for long-termconservation of perilla seeds without detrimental effects on germination.
이영이,배은주 대한약학회 2013 Archives of Pharmacal Research Vol.36 No.11
Adipocytes are the key player in adipose tissueinflammation and subsequent systemic insulin resistanceand its development involves complex process of proliferationand differentiation of preadipocytes. Fistein, apolyphenol flavonoid, is known to exert anti-inflammatory,anti-carcinogenic and anti-diabetic effects. In this study,we aimed to investigate the effect of fisetin on adipocyteproliferation and differentiation in 3T3-L1 preadipocytecell line and its mechanism of action. We found that fisetininhibits adipocyte differentiation in a concentrationdependent manner, which were evidenced by Oil Red Ostaining and the protein expression of mature adipocytemarker genes fatty acid synthase and peroxisome proliferator-activated receptor c. Moreover, the proliferation ofpreadipocytes was also markedly suppressed by treatmentof fisetin for 24 and 48 h in the differentiation medium. Wealso found that fisetin inhibition of adipocyte differentiationwas largely due to the effect on mitotic clonalexpansion. Fisetin suppression of preadipocyte proliferationat early stage of differentiation was accompanied bythe changes of expression of a series of cell cycle regulatoryproteins. Altogether, our results suggest that theinhibition of adipocyte differentiation by fisetin may be atleast in part mediated by cell cycle arrest duringadipogenesis.
초저온보존 및 인위노화 처리가 인삼 개갑종자의 발아율에 미치는 영향
이영이,이정윤,김장욱,백형진,윤문섭,Kotnala Balaraju 한국약용작물학회 2018 한국약용작물학술대회 발표집 Vol.2018 No.10
Background : Ginseng seeds are one of short-lived seeds species which loose their viability easily in the condition of conventional storage. Cryopreservation using liquid nitrogen (LN) has been recommended as a alternative storage for this kind of germplasm short lived or dessiccation-sensitive. This study was performed to find out whether cryopreservation could assure initial viability not only for seeds with high germination rate but also for seeds with low germination rate (in aging process). Methods and Results : In this work, 3 cultivars of dehisced ginseng seeds were artificial aged in the condition of 40℃, 95% RH during 6 hours with 3 hours-interval. The germination rate of ginseng seeds was decreased to the range of 71 - 94% of initial viability by artificial aging treatment. After 24 hours of vapor-LN exposure on both of artificial aged and non treated seeds as a cryopreservation, germination rate for each cultivar was decreased with the range of 76 - 95% of initial viability. While the decreasing patterns of germination rate for each cultivar showed similar curves between before and after vapor-LN exposure, the aging effects could be slightly little by cryopreservation for 3 cultivars of ginseng seeds. Conclusion : From the above results, we may suggest that cryopreservation could be recommened for storage tool of dehisced ginseng seeds even with low viability also and expected to make slower seed aging process during preservation period through further study.