Cytogenetic Characteristics of Chinese Hamster Ovarian Cell CHO-K1

Sohn, Sea-Hwan,Cho, Eun-Jung,Jang, In-Surk The Korean Society of Animal Reproduction 2006 Reproductive & developmental biology Vol.30 No.4

The Chinese Hamster Ovarian cells CHO-K1 are one of the most extensively used cells for the evaluation of gene expression and toxicology. However, these cells are frequently used for biomedical research without consideration of their cytogenetic characteristics. Therefore, we carried out to investigate the karyologic profiles, the frequency and type of chromosome aberration, and the distribution of telomeric DNA on chromosomes of the CHO-K1 cells. The GTG banding and fluorescence in situ hybridization on CHO-K1 cells were performed to characterize the karyotype and the distribution of telomeric DNA The present study revealed that the chromosome modal number of CHO-K1 cells was 2n=20; eight chromosomes appeared to be identical with those of the normal Chinese hamster, whereas the remaining 12 chromosomes were shown to be translocated, deleted, inversed, or rearranged from Chinese hamster chromosomes. The telomeric DNA on CHO-K1 chromosomes was intensively distributed at the centromeres rather than the ends of chromosomes. In addition, three chromosomes had interstitial telomeres and one marker chromosome entirely consisted of telomeric DNAs. The frequency and type of chromosome aberrations in CHO-K1 cells were examined. Of the 822 metaphase spreads, 68 (8.3%) cells resulted in chromosome aberrations of which the chromosome breakage was the most frequently occurred.

HIV 감염자에 대한 달팽이 엑기스의 혈액면역학적 효과

조영걸,이희정,오원일,강은숙,김은순,김영봉,조양자,조군제 大韓免疫學會 1996 大韓免疫學會誌 Vol.18 No.2

A unique sialic-acid-binding lectin from giant African snail Achatina fulica(AF) has a T cell mitogenicity. It was also noted that acetone extracts of AF have a anti-HIV effect in vitro. Thirty HIV-infected individuals(HIV+) were treated with AF extract(40% Chunho extract) for average 5. 5 months. Peripheral blood sampling and questionnaire were done every month after take. There was a significant decrease of WBC from 4 months after take. As a result, lymphocyte counts also decreased(P<0.01). However, there was a significant increase of CD4+T cell percentage from 1 month(P<0.01). The mean increase of CD4+T cell count was significant for first one month(39/pl)(P<0.05). Body weight also increased significantly(P<0.05). Over 90% HIV+ ex-pressed symptomatic improvement and wanted to be treated with AF extract continuously after the end of this study. In one HIV+ treated with AF for 19 months, the increases of CD4+T cell percentage and counts over 11 times after AF extract take were mean 67% and 85%, respectively, compared to baseline value. These data strongly suggest that aqueous AF extract has a immune enhancing effect on HN+.

한국전쟁과 지방화단

조은정(Cho Eun-Jung) 부산대학교 한국민족문화연구소 2010 한국민족문화 Vol.38 No.-

1950년에 발발한 한국전쟁은 식민지에서 벗어난 지 얼마 되지 않은 상태였던 한국 사회의 많은 것을 변화시켰고 미술인의 활동에도 일정 부분 변화를 가져왔다. 월북과 월남 그리고 부산으로의 피난으로 대표되는 작품활동 지역의 변화는 근거지를 잃은 작가들이 고향과 경제력을 상실하게 함으로써 개인적인 패배감을 강화시켰을 뿐만 아니라 심리적인 공황까지 야기시켜 작가 자신의 세계를 개척하기 어렵게 만들었다. 하지만 전쟁 중에도 화가들은 다방에서 개인전이나 그룹전을 열어 창작열을 불태웠으며 대한미술협회라는 단체를 결성하여 전열을 정비하기도 하였다. 그림 그릴 재료가 곤궁하고 생활이 어려운 상황에서도 미술에 열중할 수 있다는 프로의식과 예술에의 의지를 볼 수 있는 이들 전시문화에는 당시의 상황에 대응한 작가의 현실 인식이 반영되어 있다. 현실 도피로서의 순수에의 집념, 무능한 가장으로서 현실적으로 해낼 수 있는 유일한 일인 그림그리기, 그 어느 것이든 한국전쟁기 화단의 활동은 멈추지 않았는데 이는 상당히 중요하며 고무적인 일이다. 다만 이념의 갈등이 빚어낸 전쟁이고 자신의 국토에서 발발한 전쟁이지만 정작 자신들은 전쟁의 주체가 되지 못한 전쟁이 한국전쟁이었다는 뼈아픈 사실을 감안하지 않으면 안 된다. 전쟁이 건국초기에 비하여 한층 더 강화된 반공이념을 정당화시키는 상황에서 미술단체와 미술제도 그리고 개개인은 이념으로부터 자유로울 수 없었다. 이념적 성향에 따라 세속적 성공과 좌절이 결정되고 마는 한계 상황에 설 수 밖에 없게 되었던 것이다. 인적 구성의 변화는 남한 내에서도 마산에 있던 임호나 이준이 부산으로 왔고, 또 휴전과 함께 이준은 서울에서 열린 제2회 〈국전〉에 화려하게 등장하였다. 미술인의 이동은 월남이나 월북 이외에 남한 내에서도 지방에의 정착과 지방미술인의 상경으로 이루어졌다. 피난민으로서 미술가들의 위상은 거주지를 옮겼음에도 끊임없이 전시를 하거나 미술작품의 창작을 쉬지 않음으로써 미술가로서의 정체성을 유지한 것이었다. 일찍이 미술에서 그렇게 활발하게 직접적으로 중앙과 지방의 교류가 이루어졌던 적은 없었다. 미술인들은 자신이 거주하는 곳의 환경과 의미를 느꼈고 그것을 어떠한 방식이든 작품에 투사하였다. 박고석의 부산의 여러 장소에 대한 풍경이나 이중섭의 제주도 풍경이 그러한 예이다. 또한 부산에 거주하는 작가들에게 다른 지역 작가들 특히 서울에서 피난온 모더니즘 계열의 자극은 자신이 속한 지역의 색채에 대한 깊이있는 논의로 이어져 전통에 대한 관심과 민족적인 미술의 확산으로 이어졌던 것도 주목할 일이다. 지방과 중앙 혹은 피난민 화가들과 지역작가들과의 관계는 일방적 영향관계로 파악했을 때, 상대에 대한 부정으로 작용하여 문화적 교류 혹은 충격이라는 사실조차 부정하게 되는 것을 본다. 그리고 당시에조차 그러한 시각은 중앙의 모더니즘 대 지방의 전통이라는 이원적인 시각을 양산하는 배경이 되었다. The Korean War that broke out in 1950 had brought a lot of changes into the Korean society, as it was only a while after becoming decolonized. It also had affected certain parts of artists’ activities. Due to evacuating toward north or south over the border, and to Busan, artists loosing their hometown and economic strength were the changes in major regions of creating pieces. Therefore, it didn’t only enhancethe sense of defeat, but also caused psychological panic so it made the artists even more difficult to develop their own world. However, artists opened private or group exhibitions in coffee shops to express their creativity. They also formed a group called ‘Korean Association of Art’ and settled the combative fervor. Artists’culture of exhibitions reflected their cognitive reality of coping with the circumstances during that time and it proved that even with scarce art materialsand difficult living environment, they could concentrate on art as professionals and it represented their will of art. Being deeply attached to purity to avoid the reality, as being an incompetence head of a household, the only thing they would do was drawing; however, none of the reasons stopped them from participating in the world of artists during the Korean War, which was really important and encouraging. Even Korean War was aroused in Korea, Korea wasnot the main subject of the war, and it started due to ideological conflicts. Therefore, the fact that Korean War was a definite agony should not be taken out of consideration. It was not possible for artists’organization, art institutions, or individuals to express their ideology fully after the war, since the anti-communism sentiment had become even more justified, compared to initial stage of establishing a country. They had to face the limited reality where secular success and breakdown were decided by their ideological propensity. The change in formation of human footsteps had also existed in South Korea; Lim, Ho and Lee, Jun had movedto Busan from Masan, and as armistice started, Lee, Jun had remarkably exposed himself through 2n the National Art Exhibition in Seoul. The movement of artists was not only crossing over the border to South or North Korea, but some artists settled in rural regions, and some came up to Seoul from rural areas. Even changing their place of residence as refugees, artists maintained their identity by opening art exhibitions constantly and not giving up on creating artworks. There never had been that direct and active way of interchange in art between the rural and central regions. Artists were impressed by their surrounding environments and significances, thus they portrayed it in their artworks in anyway. Several scenery of Busan by Park, Go-suk, and Jeju Island’s scenery by Lee, Jung-Sup are the examples. Also, the artists of modernism line from other regions’, especially Seoul, had stimulated artists living in Busan. It should be noted that, the fact of having in-depth discussion about their regional coloration among the artists had led them to become more interested in the tradition, and it even extended the field of art. By understanding the relations between the rural and central, the refugee artists and regional artists, respectively, were unilaterally influential relations they even disagreed on the fact that it was cultural exchange or cultural shock since they impactedeach other negatively. Even during that time, that kind of viewpoint had become the background of setting the ‘central modernism vs. rural tradition’ dualistic perception.

Three-Dimensional Porous Collagen/Chitosan Complex Sponge for Tissue Engineering

Kim, Sung Eun,Cho, Yong Woo,Kang, Eun Jung,Kwon, Ick Chan,Lee, Eunhee Bae,Kim, Jung Hyun,Chung, Hesson,Jeong, Seo Young The Korean Fiber Society 2001 Fibers and polymers Vol.2 No.2

A three-dimensional, porous collagen/chitosan complex sponge was prepared to closely simulate basic extracellular matrix (ECM) constitutes, collagen and glycosaminoglycan. The complex sponge was prepared by a lyophilization method and had the regular network with highly porous structure, suitable for cell adhesion and growth. The pores were well interconnected, and their distribution was fairly homogeneous. The complex sponge was crosslinked using 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS) to increase its boilogical stability and enhance its mechanical properties. The crosslinking medium has a great effect on the inner structure of the sponge. The homogeneous, porous structure of the sponge was remarkably collapsed in an aqueous crosslinking medium. However, the morphology of the sponge remained almost intact in a water/ethanol mixture crosslinking milieu. Mechanical properties of the collagen/chitosan sponge were significantly enhanced by EDC-mediated crosslinking. The potential of the sponge as a scaffold for tissue engineering was investigated using a Chinese hamster ovary cell (CHO-K1) line.

하루 콩단백질 25g 섭취를 위한 메뉴작성 및 영양성분 분석

한재숙,김정애,서봉순,이연정,서향순,조연숙,한경필,이신정,오옥희,우경자,조은자,구성자,김수진,李承彦,南出隆久 동아시아식생활학회 2002 동아시아식생활학회지 Vol.12 No.2

The purpose of this study was to develop menus for daily intake of 25g soybean protein and to analyse nutrients of these foods. Analytical values were compared to the theoretical one using the food composition table and recommended dietary allowances for Koreans (7th revision). The results are as follows. 1. Soybean curd residue stew, Soybean curd, Kimchi saute, and hard boiled soybean and lotus root were selected for the menu for January, of which the content of soy bean protein(SBP) was 33.1g, soybean stew, soybean curd and soybean sprout saute, and Italian deep fried soybean curd were for February, of which the content of SBP was 35.0g. The content of SBP in soybean paste soup with soybean curd, fried soybean curd and fried soybean curd roll, the menu for March, was 24.9g. That of April were soybean curd gratin with soymilk, soybean curd and ham with garlic dressing and the content of SBP was 26.3g. That of May were soybean porridge, soybean flour cake with honey (Dasik), soybean sprout soup and the content of SBP was 26.7g. That of June were soymilk, pan-fried soybean curd, steamed soybean curd with chicken and the content of SBP was 28.4g. That of July were noodle with soymilk, mapatofu, soybean curd salad and the content of SBP was 24.7g. That of August were soybean sprout with mustard dressing, Tossed green pepper with raw soybean flour, Tofu and Kimchi stew, soybean curd steak and the content of SBP was 26.2g. That of September were Chinese cabbage soup with raw soybean flour, sweet and sour tofu and the content of SBP was 23.2g. That of Oct. were Fermented soybean stew, soybean pan cake and the content of SBP was 24.3g. That of November were not-pressed soybean curd casserole, pan-fried mashed soybean curd with egg, stir frying deep-fried soybean curd with vegetables and the content of SBP was 22.4g. That of December were soybean curd and mushroom casserole, fried soybean curd and vegetables, hard boiled soybean curd and the content of SBP was 28.9g. 2. The ratio of the analytical value over theoretical value (A/B%) of one serving in kcal, carbohydrate, protein, fat, ash and dietary fiber were 57.7~107.7%, 42.9~131.9%, 79.2~118.3%, 54.5~100%, 40.7~80.8% and 42.1~113.2%, respectively. 3. The ratio of A/B% of one serving in Ca, K, Na, P and Fe were 44.1~93.6%, 59.0~153.1%, 53.1~117.7% 64.6%~138.8 and 33.8~77.3%, respectively. That in Fe was the lowest among minerals. 4. The ratio of analytical value over Korean R.D.A(A/C%) in Ca was relatively higher (22.0~85.9%) than that in kcal(18~63%). 5. The ratio of A/C% in Fe was 25.1~64.3% and lower than that in Ca and protein in general.

Minoxidil에 의한 모발절대휴지기 탈출 유도 효과

이은정,조영롱,조경혜 서울여자대학교 자연과학연구소 2011 자연과학연구논문집 Vol.23 No.-

Hair follicle (HF), a small physiological organ in the human body which produces hair, repeats a four-phased hair cycle consisting of anagen, catagen, telogen, and exogen phases and affects the growth, maintenance, and loss of hair. Recent studies revealed that the hair growth and loss are controlled by the balance between bmp and nog expressed from dermal papilla cells (DP cells) which control the physiological characteristics of the hair growth cycle and that a phase transition from refractory telogen phase to competent telogen phase, caused from the reduction in expression of bmp gene in the telogen phase, is indispensible for the regeneration of hair cycle. This study is conducted to verify the effect of minoxidil, a depilatory lotion widely used around the globe on the molecular mechanism in the hair cycle process occurring in the DP cells, especially in the expression of bmp and nog genes. DP-enriched cells were separated and cultured from the back skin of 1 day-old new born rats and observed the effects after processing minoxidil on the cells. Minoxidil reduced the expression of bmp4 and bmp6 genes, which stimulate differentiation but inhibit multiplication of DP cells in the hair cycle control process, and reduced the expression of bmprla gene, as its receptor. Minoxidil also reduced the expression of nog genes known as an antagonist of bmp produced from DP cells and an essential element in the telogen-anagen phase transition. From this results, minoxidil induced the DP cells to stay at the competent telogen phase, a prior phase of the initiation of the anagen phase, and to escape from refractory telogen phase of the hair cycle. Also, minoxidil induced DP cells to an ‘undifferentiated state of hair cell' of competent telogen phase, a prior phase of early anagen phase, rather than that inducing the cell to an initiation phase of hair cycle, the anagen phase. Minoxidil reduced the expression of nog gene which is an essential element in the regeneration of hair cycle, but increased the expression of bfgf gene, a upstream growth factor which stimulates the expression of nog and vegf genes which foster the growth of DP cell. Thus, it can be suggested that there is possibility that minoxidil indirectly affects telogen-anagen phase transition and the initiation of anagen phase. In conclusion, this study verified that minoxidil leads escape from refractory telogen phase of a hair growth cycle and thereby reduces the period of the telogen phase. Key words:dermal papilla cells, minoxidil, bmp, noggin, hair cycle.

재활승마의 효과에 대한 고찰

조경희,김은정,정재민,황보각 대구대학교 특수교육재활과학연구소 2010 再活科學硏究 Vol.28 No.1

이 연구는 승마 운동의 효과에 대한 객관적이고 구체적인 정보를 제공하기 위해 승마운동이 균형, 자세, 골밀도, 근력, 정서에 어떤 영향을 미치는지 알아보았다. 최신 동향을 살펴보기 위해 90년대 이후의 자료만을 선택하여 승마운동, 승마치료, 재활승마 등 재활 승마와 관련된 키워드를 검색하고 승마운동을 통한 효과를 알아보기 위해 균형, 안정성, 골밀도, 근력 등의 연관 검색어를 함께 검색하였다. 그 결과 지속적인 승마운동은 균형 능력 향상을 위해 효과적인 운동이며 대퇴전자부와 대퇴경부의 좌우측, L3,L4의 골밀도를 향상시키고 승마에서만 발달될 수 있는 특수한 부위를 자극한다. 또한 재활승마를 통해 인지적, 사회적인 부분에 대해 눈에 띄는 향상을 기대할 수 있다. The purpose of this study was to provide information which objective and definite effect of horseback riding about balance, posture, bone mass, muscle strength, emotion. To search the newest trend, Studies for this literature review were sought which investigated the effect of HBRT, hippotherpay, therapeutic horseback riding since 1990. These literature searches were also linked to other descriptive terms. For example, balance, posture, bone mass, muscle strength, emotion. Continuos horseback riding therapy is effective exercise to improve balance ability and bone mass of femur trochanter, femur neck, lumbar 3-4. Also, horseback riding can expect to improve recognition and sociality.

학교행정가의 리더십과 핵심역량에 대한 인식 연구

조경원,한유경,서경혜,조정아,이지은 이화여자대학교 교육과학연구소 2006 교육과학연구 Vol.37 No.1

21세기 지식정보화 사회는 전통적인 학교교육의 변혁을 요구한다. 이에 학교교육 개혁을 성공적으로 이끌어 나아갈 수 있는 교원의 리더십 특히 학교행정가의 리더십이 절실히 요구되고 있다. 그렇다면 지식정보화 사회에서 요구되는 학교행정가의 리더십이란 무엇이며 어떤 역량을 포함하는가? 본 연구는 학교행정가의 리더십과 핵심역량에 대한 선행연구가 학교 현장과는 유리된 채 이론적, 당위적 수준에 그치고 있다는 문제의식에서 출발하여 학교행정가의 리더십을 구성하는 핵심역량이 무엇인가에 대한 교사, 교감, 교장, 행정실장 및 서무부장의 인식을 파악하고 이들 집단의 인식을 비교 분석하는 데 목적을 두었다. 이를 위해 우선 문헌연구를 통해 리더십에 대한 관점과 개념의 변화를 고찰하였고 변혁적 리더십 관점에 기초하여 학교행정가가 갖추어야 할 핵심역량으로 교육적 리더십, 학교 계획 및 조직, 학교교육과정 편성 및 운영, 학교운영, 인적자원 개발, 학교재정 관리, 교육법 이해 및 집행, 학교교육정책 이해, 학교·학부모 및 지역사회 협력, 정보통신기술 활용과 같은 열 가지 역량을 도출하였다. 문헌연구를 통해 도출된 핵심역량에 대한 학교 현장의 관점을 알아보기 위해 초·중·고등학교 교직원을 대상으로 10대 핵심역량의 중요도와 현재 실행정도에 대해 설문조사를 수행하였다. 설문조사에는 144개 학교의 교직원 1,136명이 참여하였다. The purpose of this study is to investigate the perspectives of teachers, vice-principals, and school staff on school administrators' leadership and core abilities. For this purpose, first, we examined the perspectives and concepts of school administrator's leadership and identified 10 core abilities that constitute the school administrator's leadership. Second, we developed a questionnaire to investigate the perspectives of teachers, vice-principals, and school staff on to what extent the 10 core abilities are important and whether their school administrators actually exert these 10 core abilities. Finally, we conducted a survey research, including teachers, vice-principals, and school staff with various years of experiences in elementary and secondary schools in Korea. The findings show that teachers, vice-principals, principals, and school staff consider the 10 core abilities very important for school administrators. However, they perceive that school administrators, in reality, do not exert the 10 core abilities as much as they think these abilities are important. In particular, teachers rated school administrators' leadership relatively low compared to principals, vice-principals, and school staff. The difference between their perception on the importance and exertion of 10 core abilities indicates the need for programs that promote the development of 10 core abilities for school administrators and consequently support the development of school administrator's leadership.

노인의 주간보호센터 이용 만족도와 생활 만족도

조은정,이숙현,전혜정 연세대학교 교육대학원 2002 연세교육과학 Vol.50 No.-

The purpose of this study was to examine the relationship between utilization satisfaction with day-care center of the elderly and their life satisfaction. The subjects were 94 older adults who were participants in one of seven day-care centers for the elderly around Seoul and who were over the age of 65 (34 older men and 60 older women). A 18-item composite index, modifying Seo's (2000) Assessment Index for the Elderly Welfare Facility, was used to measure the satisfaction with elderly day-care center. Life satisfaction of the elderly was assessed using Yoon's Criterion of Older Adult's Life Satisfaction. Data were analyzed with t-test, analysis of variance and Pearsons' correlation. The major results were as follows: First, most respondents used the center for more than two years, and they visited the center 3-4 times per week. Most of the elderly used the center for health recovery and they recognized that their health improved after using the facility. Medical treatments and physical body service programs were preferred among programs operating in the facility. Second, utilization satisfaction was found to be relatively high. The satisfaction with the elderly day-care center varied with older adults' subjective view on their health condition, monthly allowance, religions, and age. The motive to use the facility was also found to be associated with the level of satisfaction. Voluntary participants reported higher level of satisfaction than non-voluntary participants. Third, there was positive association between utilization satisfaction and life satisfaction. Life satisfaction of the elderly who use day-care center was relatively high.

silver-age의 web-interface에 관한 연구 : 한국의 silver-age를 중심으로

조은정,이광호 건국대학교 조형연구소 2001 조형연구 Vol.9 No.-

Times have changed. And come to the time one man, one computer. Our social environment, into the web. And don't five about one-day without computer, silver-age is the Informal An elderly person degeneration of body and change state of mind. From social develop, grow a matter of concern and interest about silver-age, and for welfare facilities about silver-age. Now recent universalization of the web, the design has change to satisfy the user's needs, breaking from the convention supply-oriented concept, as a result of which the concept of the interface has spreaded all over the society. This change been on the level to provide users with ease and convenience in use. Saying that creating a Web is design is not exaggeration. A huge new market is being born. Mastering digital technology can be said to be a new requirrment for designers to survive in the profession. A method of study started for compose web-interface about silver-age, if they are use the web, he have to thinking. It is convenient. For welfare measures is as follows about silver-age. first, health-home and the spirit of filial piety and respect, second, social security one's old age, third, the health system for silver-age, last, social-activity. For that reason a necessary and sufficient condition use the web, with silver-age. So, this thesis's goal is research web site for silver-age, and offer convenience use computer.