Casein micelle 의 조성에 관한 연구 : I. Micelle 크기 별에 따른 각 casein 의 구성비율 I. The relative amounts of individual caseins in the different casein micelles

김영교,신동철,전우민 한국낙농학회 1984 韓國酪農學會誌 Vol.6 No.1

本 實驗은 casein miceile의 크기에 따른 각종 casein의 構成成分을 究明하고자 超遠心分離機를 利用하여 casein micelle을 크기 別로 分類하고 DEAE-cellulose column chromatography를 이용하여 각 casein의 構成比率을 조사하였다. 그 結果를 要約하면 다음과 같다. 1. 脫脂乳를 100,000×g에서 각각 30分, 60分, 90分동안, 200,000×g에서 60分동안 遠心分離하였을 때 沈澱되는 casein micelle 量은 45.00%, 61.43%, 80.00% 및 95.00%였으며 沈澱되지 않는 serum casein量은 55.00%, 38.57%, 20.00% 및 5.00%였다. 2. 脫脂乳를 100,000×g에서 時間 差에 따라서 遠心分離하였을 때 각각 沈澱되는 casein micelle量은 10分間에는 全 casein量의 23.13%, 11∼30分間에는 30.26%, 31∼60分間에는 28.11%였으며, 60分問의 遠心分離에 의해서 沈澱되지 않는 serum casein은 17.79%였다. 3. 時問 差로 遠心分離하여 얻어진 casein micelle중 각 casein의 構成比率을 測定한 結果, α_s-casein, β-casein, κ-casein 및 γ-casein의 상대적인 量은 100,000×g, 10分의 pellet에서 각각 54%, 29%, 11% 및 6%였고, 100,000×g, 11∼30分의 pellet에서는 각각 54%, 28%, 13% 및 5%였고, 100,000×g, 31∼60分의 pellet에서는 각각 51%, 28%, 16% 및 5%로 나타났으며, serum casein은 44%, 29%, 19% 및 8%로 각각 나타났다. This experiment was carried out to study on the protein composition of casein micelles. Three micellar casein pellets and a serum casein were isolated at diferent time of ultracen trifugation. And the composition of individual caseins for the fractions was determined by DEAE-cellulose column chromatography. The results are summarized as follows: 1. When skimmilk was centrifuged for 30 min, 60 min and 90 min at 100,000g and for 60 min at 200,000g, the values for the sedimented casein micelles were 45.00%, 61.43%, 80.00% and 95.00% respectively and that of serum casein was 55.00%, 38.57%, 20.00% and 5.00% respectively. 2. When skimmilk was centrifuged for different time at 100,000g, the values for the sedimented casein micelles were 23.18% for 10 min.(pellet 1), 30.26% from 11 to 30 min.(pellet 2), 28.11% from 31 to 60 min.(pellet 3) and that of serum casein which was not sedimented at 60 min was 17.79% of the total casin. 3. The relative amounts of α_s-, β-, k- and γ-caseins in casein micelles obtained after different time of centrifugation were 54%, 29%, 11% and 8% in pellet 1, 54%, 28%, 13% and 5% in pellet 2, 51%, 28%, 16% and 5% in pellet 3,44%, 29%, 19% and 8% in serum casein respectively.

대장내시경검사의 전처치로서 항콜린제 사용에 관한 연구 : 전향적, 이중맹검법으로

박경남,한동수,이민호,최호순,박준용,손주현,이오영,함준수,전용철,송승찬,기춘석,윤병철,이종희 대한소화기내시경학회 1997 Clinical Endoscopy Vol.17 No.3

Background: Use of antispasmodic medication prior to colonoscopy is controversial but someone believes antispasmodic may improve visualization of colonic mucosa and ease colonoscope insertion. So, we designed a study to assess the effect of premedication with the antispasmodic, hyoscine-N-butyl bromide(Buscopan') on the performance of colonoscopy. Methods: This study was prospective, double blinded, randomized, controlled study, One hundred three consecutive patients were randomized to receive intravenous buscopan lml(n=52) or placebo(n=51) combined with our standard initial medication(me- peridine 50 mg and midazolam 2 mg). Insertion of colonoscopy was timed, and 100 mm visual analogue scales (VAS) were used for asscssing difficulty of procedure, colonic motility, frequency of positional change, frequency of external compression, difficulty of assistance and degree of discomfort experienced by the patients. Results: There were no significant differences of intubation time between buscopan group(mean time, 7.23 min., range 2~15) and placebo group(7.07 min., range 3-25), (p=0.83) and withdrawal time between buscopan group (6.46 min., range 2-22) and placebo group(6.76 min., range 2 25), (p=0.69). Also, there was no significant differences in intubation time between males and females(buscopan; males 7.00 min., females 7.60 min., p=0.34, placebo; males 7.0~5 min., females 7.08 min., p 0.44). The VAS scores checked by endoscopist(p=0.29), assistant(p=0.32) and patient (p=0.15) were not significantly different in both groups. There were no significant differences in intubation time, VAS scores nf endoscopist, assistant, and patients. Conclusion: Premedication with intravenous bu.opan has no advantage on colonoscopy procedure. Use of antispasmodic medication prior to colonoscopy was not considered as recommendable agent.

體育硏究에 社會學이 미치는 影響 : Its range and methodology

田東善,閔昌基 충남대학교 자연과학연구소 1977 學術硏究誌 Vol.4 No.2

Sociology of Physical Education is defined as a science whose function is to study from the social viewpoint, with correct understanding of the fact of physical Education as that of education and sociology. As a result of the study on the influence of Sociology on studies of physical Education-its range and methodology, the following results have been obtained: 1. The contents of physical Education is concerned with a) social behavior b) group c) culture and system d) influence of social change etc. 2. Its range covers a) culture of physical education b) group of physical education c) facilities of physical education d) guidance and teaching of physical education e) system of physical education etc. 3. If physical education is regarded as a combination of aspect of natural science with that of mental science, its physical activities tend to be realistic and practical rather than social and scientific. Physical education, therefore, must be pursued from the viewpoint of social and scientific aspect. 4. Sociology of physical education, a newly developed field of study, has made a great contribution to the study of physical education as a fundamental field. It is essentially required that its desirable methodology be developed and studied. At this moment, however, the methodologies of related sciences should be introduced under the recognition of its originality and it is deemed that researches on this matter should be made persistently so that physical education can contributed to the academic systematization and scientification of physical education.

지형학적 인자에 의한 미호천수계의 유황분석

전민우,최동해,조용수 충북대학교 건설기술연구소 2002 建設技術論文集 Vol.21 No.2

The correlation equations were proposed to predict the flow duration of ungaged basin by regression analysis based on geomorphological factors. The proposed correlation equations give good results in consequence of application in Kagyung stream basin and Samki stream basin. Among the flow duration, the correlation coefficient of fruitful water discharge was the smallest value, on the hand, the correlation coefficient of reference low water flow was appeared to be the largest value. The correlation coefficient including only stream slope S as the independent variable was the smallest value, on the other hand, in the case of including basin area A and stream length L or A, L, S as the independent variable the correlation coefficient appeared to be the largest value and in this case, the two correlation coefficient were very close. These proposed correlation equations can be used to grasp the flow duration by acquiring the geomorphological factors of ungaged stream basin.

여수석유화학산단 내 VOCs에 대한 오염원 분류표의 개발 및 CMB 모델에 의한 기여도 산정

전준민,허당,김동술 한국대기환경학회 2005 한국대기환경학회지 Vol.21 No.1

The purposes of this study were to characterize the local levels of VOCs (volatile organic compounds), to develop source profiles of VOCs, and to quantify the source contribution of VOCs using the CMB (chemical mass balance) model. The concentration of VOCs had been measured every 6-day duration in the SRO monitoring site in the Yeosu Petrochemical Industrial Complex from September 2000 to August 2002. The total of 35 target VOCs, which were included in the TO-14 designated from the U.S. EPA, was selected to be monitored in the study area. During a 24-h period, the ambient VOCs were sampled by using canisters placing about 10~15 m above the ground level. The collected canisters were then analyzed by a GC-MS in the laboratory. Aside from ambient sampling at the SRO site, the VOCs had been intensively and massively measured from 8 direct sources and 4 general sources in the study area. The results obtained in the study were as follows; first, the annual mean concentrations of the target VOCs were widely distributed regardless of monitoring sites in the Yeosu Petrochemical Industrial Complex. In particular, the concentrations of BTX (Benzene, Toluene, Xylene), vinyl chloride were higher than other target compounds. Second, based on these source sample data, source profiles for VOCs were developed to apply a receptor model, the CMB model. Third, the results of source apportionment study for the VOCs in the SRO Site were as follows; The source of petrochemical plant was apportioned by 31.3% in terms of VOCs mass. The site was also affected by 16.7% from wastewater treatment plant, 14.0% from iron mills, 8.4% from refineries, 4.4% from oil storage, 3.8% from automobiles, 2.3% from fertilizer, 2.3% from painting, 2.2% from waste incinerator, 0.6% from graphic art, and 0.4% from gasoline vapor sources.

여수산단 대기 중 휘발성유기화합물질 농도 경향

전준민,허당,김동술 한국대기환경학회 2003 한국대기환경학회지 Vol.19 No.6

The concentrations of volatile organic compounds (VOCs) in the ambient air were measured at three sites (Samil-dong, SRO and EFMC) in Yeosu industrial complex during September 2000 to August 2001. Air samples were collected for 24 hours in Silicocan canister (6ℓ) with constant flow samplers every 6 days and analyzed using a cryogenic preconcentration system and a GC/MS. At each site, we identified 35 species known as on both the carcinogenic and mutagenicity by the EPA US (TO-14 manual). No relationship was found between VOCs concentration at three sampling points. Furthermore, the result shows that there appears to be a variety distribution of the concentration. BTX, vinyl chloride and high concentrations of I, 2-dichloroethane were observed at the sampling sites. Especially, high concentration of toxic VOCs, such as vinyl chloride, chloroform, I, 2-dichloroethane and benzene were shown at environmental facilities management cooperation (EMFC) site. They seem to be emitted from the facility of wastewater treatment in Yeosu industrial complex. It was difficult to tell the seasonal variation of total VOCs concentration. Nevertheless, the concentration in winter was typically higher than in summer. The concentrations of toxic VOCs contents in Yeosu industrial complex were generally lower than those in Ulsan complex, although those were similar or less than in Seoul and Daegu. Whereas, toluene and styrene emitted from Yeosu industrial complex were higher than those of Edmonton industrial complex in Canada. Especially, toluene was third times higher than those observed from Texas, USA.

황색 포도상구균의 vancomycin 내성 원인 유전자의 동정

전준연,양영기,송민동 조선대학교 부설 유전생물공학연구소 1991 유전생물공학연구지 Vol.1 No.1

임상 분리되는 Gram 양성균인 황색 포도상구균(Staphyhcoccus aureus)에 대한 각종 학생물질의 최소 발육저지농도 측정(Minimal Inhibitory Concentration: MIC test)과 내성 pattern을 조사하였으며, 이들 균주 중 특히 vancomycin에 대하여 높은 내성을 나타내는 균주(Vancomycin resistant Staphylococcus aureus: VRSA)에 대한 vancomycin 내성원인 유전자의 탐색 및 cloning을 실시하여 vancomycm 내성기구의 연구에 필요한 조사를 수행하였다. Vancomycm에 내성인 균주(VRSA)를 고온(43.5℃) 처리하여 계대 배양을 한 다음, replica plating method에 의 해 vancomycin 감수성 균주(Vancomycin-sensitive Staphylococcus aureus: VSSA)를 얻었으며, VRSA 균수와 VSSA 균주로부터 membrane protein을 준비한 다음 SDS-poly-acrylamide gel electrophoresis(SDS-PAGE)로 전기 영동하여 그 단백질 pattern을 비교한 결과, VRSA 균주에서는 분자량(Mr: relative molecular weight)이 약 127Kadl 그리고 30Kdal 정도 되는 부위에서 vancomycin 내성에 관계되는 듯한 특이적인 단백질을 관찰할 수 있었다. 그러나 VSSA 균주에서는 이러한 단백질을 관찰할 수 없었다. 한편 이들 균주로부터 plasmid DNA를 분리한 다음 agarose gel electrophoresis하여 그 pattern을 분석한 결과, VRSA 균주에서는 약50Kb부위에서 plasmid DNA밴드가 관찰되었으나 VSSA 균주에서는 아무런 밴드도 관찰되지 않았다. 이상의 결과로 볼때 vancomycin 내성유전자는 plasmid DNA상에 존재한다고 생각되어진다. This study was carried out to study the characterization of the gene that is responsible for the vancomycin-resistant Staphylococcus aureus (VRSA) In order to obtain vancomycin-sensitive S. aureus(VSSA)from VRSA stain, vancomycin-resistant S. aureus(VRSA) was subcultured twice at the high temp.(43.5℃)and then VSSA was obtained by replicaplating method after MIC test with vancomycin in high temp. treted VRSA strain. Membrane proteins were prepared from VRSA and VSSA strain and protein patterns of those were compared bny 7.5% SDS-polyacrylamide gel electrophoresis. Only in VRSA strain appeared novel protein bands at a position about 120 Kdal, 80 Kdal and 30Kda1, but it did not appear in VSSA strain. The patterns of plasmid DNA in VRSA and VSSA strain were clearly classified. Plasmid DNA band in VSSA strain appeared at a position about 50Kb, but it did not appear any band in VSSA strain. These results suggested that plasmid DNA may be encode the gene that is responsible for vancomycin-resistance.

설전절제술후 유리피판술을 이용한 설재건술의 기능적 평가

선동일,김민식,이정학,전진하,송치준,조승호 대한이비인후과학회 2000 대한이비인후과학회지 두경부외과학 Vol.43 No.10

씨름선수의 정체성 형성에 관한 생애사적 연구

전민주,박기동 江原大學校附設體育科學硏究所 2003 江原大學校附設體育科學硏究所論文集 Vol.- No.26

The purpose of this research is to investigate the identity development process of an athlete in his life history and to understand its constituent elements as well as the interaction of them. In the study on the socialization of mountaineers and Rugby players, Donnely and Young(2001) divided the process into four stages; Pre-socialization stage, Selection and recruitment stage, Socialization stage, Acceptance and ostracism stage. One Ssireum player from H Univ. is selected as a subject of study through Ideal Case Selection which is one of Criterion-based Selection methods. The relevant materials are collected by various means such as in-depth interview, participatory observation, documentary study. According to the general procedure of quality analysis, the data can be arranged through transcription and categorical coding process. The methods of inter-members' checking and peer debriefing are put to use in order to verify the reliability and validity of the data and to validate their trustworthiness and refinement. The results of the study are classified by the question of study as below. What is the conditions of identity as a Ssireum player? Through what process does a man build up the identity? The identity formation of a Ssireum player is formed not by an individual and one-way process but by the mutual relationship between the informer and his surrounding culture, and the relationship between him and fellow members. Furthermore, the mode of change is not simple but consistent and active. The identity of a player become established by going through Pre-socialization stage, Selection and recruitment stage, Socialization stage, Acceptance and ostracism stage, respectively. Pre-socialization stage is one within which a man does not yet become a player. Therefor, this stage is less systematic and less organized than the later stages and can be said to be a germinal stage. Selection and recruitment stage is an introductory one in which a player is initiated into a certain culture by way of the encouragement of his teacher. To become a member of an athlete club is to experience the Ssireum practice other than before. Socialization stage is a phase of new role performance and new identifications. A player associates with the other members and experiences consistent athlete practices through all this phase. He adapts himself to the school life in which he determines his aim. He inherits the others experiences and skills from which he forms a new cultural history on his own. The informer of this study underwent the early three stages and their entire features is almost typical. As a senior student, he came to the fourth stage, namely, Acceptance and ostracism stage, in which he tries to escape from the uniform society of the insistent challenges. As the school, he thinks, emphasized the public relations and the actual results, he also considered the school as a means to personal success. Then, only in this last phase, he feels sorry for his stipulation in which he regards himself only as an athlete. He tries ardently to restore the spirit of the student.

씨름선수의 정체성 형성과정 가운데 격리화 양상에 관한 생애사적 연구

전민주,박기동 한국체육사학회 2004 체육사학회지 Vol.13 No.-

The purpose of this research is to investigate the identity development process of an athlete in his life history and to understand its constituent elements as well as the interaction of them. One Ssireum player from H Univ. is selected as a subject of study through Ideal Case Selection which is one of Criterion-based Selection methods. The relevant materials are collected by various means such as in-depth interview, participatory observation, documentary study. According to the general procedure of quality analysis, the data can be arranged through transcription and categorical coding process. The methods of inter-members' checking and peer debriefing are put to use in order to verify the reliability and validity of the data and to validate their trustworthiness and refinement. The results of the study are classified by the question of study as below. There are influential elements on the identity formation such as human relationship, group consciousness, role-playing, seniors' attitudes, conventional manners. The relationship between these elements and his own consciousness makes shape his identity, forming his self image directly and indirectly. As one member of an athlete club, the Ssireum player becomes separated from common students in respect of time, space, and group associates, which are determining elements of athlete's identity formation The athlete not only receives his images from without but also substitutes them with his own images and undergoes the process of refinement and accommodation. The kind of trainers, the nature of an athlete group he turns to, and the feature of the role-playing performance are all affecting the identity formation The surrounding circumstances, which include practice establishment, living environment and management of personal behavior, are integral, too. Athlete's self-realization is closely related with the surrounding cultural frame and the discrimination mechanism within it.