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우리나라의 철강산업에서 대기오염의 관리 및 방지현황에 관한 연구
김동술,김신도,유정석 경희대학교 환경연구소 1994 環境硏究 論文集 Vol.5 No.-
국가 경제발전에 크게 기여하고 있는 철강산업은 에너지를 과소비하며, 폐기물을 대량 발생하는 공해 유발업종이기도 하다. 1970년 이후 철강산업은 지속적인 산업발전을 위하여, 생산설비의 확장과 함께, 대기의 질을 개선시키기 위하여 방지설비에도 많은 비용을 투자하였다. 대부분 산업체의 경우처럼, 국내 철강산업 역시 개발과 환경보존과의 조화라는 어려움을 가지고 있었으며, 각종 대기환경정책의 변화에도 적극적으로 대처하고 있다. 본 연구는 철강산업의 국가 경제발전에 주요 공헌산업으로서의 역할을 계속 유지하며, 철강산업이 국내 환경개선에도 선도적인 역할을 수행할 수 있도록, 우리실정에 적합한 대기오염 배출규제의 방향을 제시하고자 하였다. 이를 위해, 본 연구는 철강산업의 환경현황과 문제점, 대기오염 방지시설의 현황과 처리에 대한 경제성 등을 연구조사하였으며, 우리나라 대기환경규제의 비합리성을 지적하였다.
ELISA법에 의한 mouse의 혈청 및 조직중의 T-2 toxin의 검색
김동술,송재영,정덕화 한국환경보건학회 1996 한국환경보건학회지 Vol.22 No.1
In order to detect the T-2 toxin accumulation in the animal tissues, T-2 toxin, produced by Fusarium sporotrichioides M-1-1, was injected to mouse by 0, 1 and 2 mg per kilogram of body weight, respectively, and T-2 toxin extracted from serum and organs were analyzed by the indirected competitive ELISA. The indirect competitive ELISA established in the laboratory can be check less than 0.1 ppb level of T-2 toxin and average recovery of T-2 toxin spiked was 80~113% in animal samples such as serum, liver and kidney. After 6 weeks of treatment with 2 mg of T-2 toxin per kg body weight, T-2 toxin was accumulated in serum (133.0 ng/ml), liver(1.4 ng/g) and kidney(14.3 ng/g) of mouse injected with 2 mg of toxin per kg body weight
Ochratoxin A가 마우스의 장기에 축적 정도와 ELISA법 확립에 관한 연구
김동술 한국식품위생안전성학회 1995 한국식품위생안전성학회지 Vol.10 No.4
Ochratoxin A was produced from Aspergillus ochraceus ATCC 18472 which was then orally administered into the experimental mice to study the toxic levels of ochratoxin A. AELISA (enzyme-linked immunosorbent assay) method which is more rapid and safe than conventional analytical method, was developed by using ochratoxin A antibody. This method was successfully used to measure the levels of ochratoxin Ain blood, liver and kidney of mice. In order to produce a large amount of ochratoxin A to study toxicity in the mice, Aspergillus ochraceus ATCC 18412 was incubated in the rice medium and as a result 0.5 g of ochratoxin A form rice medium (kg) was produced after extraction and purification Feed consumption and gain in body weight of mice with ochratoxin A at a level of $10 \mu\textrm{g}/g$ body weight was significantly (p<0.05) reduced as compared with control during period of 3 weeks. Ochratoxin A-BSA conjugate was made by putting 13 mole of ochratoxin A on I mole of BSA. This conjugate was used to develop ELISA method. The minmum detection level of ochratoxin A by established ELISA method was 0.5 ppb. After oral adminstraton of ochratoxin A dose of $10\;\mu\textrm{g}/g$ every two day for 3 weeks, concentration of ochratoxin A was measured in the blood, liver and kidney by ELISA method. The level of ochratoxin A was $11\;\mu\textrm{g}/dl,\;0.9 \mu\textrm{g}/g\;and\;3.7\;\mu\textrm{g}/g$ in the blood, liver and kidney, respectively.