Variants on ESR1 and their Association with Prostate Cancer Risk: A Meta-analysis

Ding, Xiang,Cui, Feng-Mei,Xu, Song-Tao,Pu, Jin-Xian,Huang, Yu-Hua,Zhang, Jiang-Lei,Wei, Xue-Dong,Hou, Jian-Quan,Yan, Chun-Yin Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.8

Background: Epidemiological studies evaluating the association of two variants rs9340799 and rs2234693 on estrogen receptor 1 (ESR1) with prostate risk have generated inconsistent results. Methods: A meta-analysis was here conducted to systematically evaluate the relationship of these two variants with prostate cancer susceptibility. Results: For rs9340799, heterozygosity of T/C carriers showed a significant increased prostate cancer risk with a pooled odds ratio (OR) of 1.34 (95% CI = 1.06-1.69) while homozygote C/C carriers showed an increased but not statistically significant association with prostate cancer risk (pooled OR = 1.29, 95% CI = 0.94-1.79). Compared to the homozygous TT carriers, the allele C carriers showed a 31% increased risk for prostate cancer (pooled OR = 1.31, 95% CI = 1.06-1.63). No significant association between the rs2234693 and prostate cancer risk was found with the pooled OR of 1.15 (95% CI = 0.97-1.39, T/C and C/C vs. T/T) under the dominant genetic model. Compared to the homozygote T/T carriers, the heterozygous T/C carriers did not show any significantly different risk of prostate cancer (pooled OR = 1.13, 95% CI = 0.94-1.36) and the homozygous C/C carriers also did not show a significant change for prostate cancer risk compared to the wide-type T/T carriers (pooled OR = 1.26, 95% CI = 0.98-1.62). Conclusion: These data suggested that variant rs9340799, but not rs2234693, on ESR1 confers an elevated risk of prostate cancer.

Comparison of carcass and meat quality traits between lean and fat Pekin ducks

Ding, Si-Ran,Li, Guang-Sheng,Chen, Si-Rui,Zhu, Feng,Hao, Jin-Ping,Yang, Fang-Xi,Hou, Zhuo-Cheng Asian Australasian Association of Animal Productio 2021 Animal Bioscience Vol.34 No.7

Objective: According to market demand, meat duck breeding mainly includes 2 breeding directions: lean Pekin duck (LPD) and fat Pekin duck (FPD). The aim of the present study was to compare carcass and meat quality traits between 2 strains, and to provide basic data for guidelines of processing and meat quality improvement. Methods: A total of 62 female Pekin ducks (32 LPDs and 30 FPDs) were slaughtered at the age of 42 days. The live body weight and carcass traits were measured and calculated. Physical properties of breast muscle were determined by texture analyzer and muscle fibers were measured by paraffin sections. The content of inosine monophosphate (IMP), intramuscular fat (IMF) and fatty acids composition were measured by high-performance liquid chromatography, Soxhlet extraction method and automated gas chromatography respectively. Results: The results showed that the bodyweight of LPDs was higher than that of FPDs. FPDs were significantly higher than LPDs in subcutaneous fat thickness, subcutaneous fat weight, subcutaneous fat percentage, abdominal fat percentage and abdominal fat shear force (p<0.01). LPDs were significantly higher than FPDs in breast muscle thickness, breast muscle weight, breast muscle rate and breast muscle shear force (p<0.01). The muscle fiber average area and fiber diameter of LPDs were significantly higher than those of FPDs (p<0.01). The muscle fiber density of LPDs was significantly lower than that of FPDs (p<0.01). The IMF of LPDs in the breast muscle was significantly higher than that in the FPDs (p<0.01). There was no significant difference between the 2 strains in IMP content (p>0.05). The polyunsaturated fatty acid content of LPDs was significantly higher than that of FPDs (p<0.01), and FPDs had higher saturated fatty acid and monounsaturated fatty acid levels (p<0.05). Conclusion: Long-term breeding work resulted in vast differences between the two strains Pekin ducks. This study provides a reference for differences between LPD and FPD that manifest as a result of long-term selection.

Prognostic Value of MAC30 Expression in Human Pure Squamous Cell Carcinomas of the Lung

Ding, Hui,Gui, Xian-Hua,Lin, Xu-Bo,Chen, Ru-Hua,Cai, Hou-Rong,Fen, Yan,Sheng, Yun-Lu Asian Pacific Journal of Cancer Prevention 2016 Asian Pacific journal of cancer prevention Vol.17 No.5

Recent evidence haas indicated that meningioma-associate protein (MAC30) exhibits different expression patterns in various tumors. However, little is known about the value of MAC30 in human squamous cell carcinoma of lung (SQCLC). The purpose of our study was to investigate the expression of MAC30 and to explore its clinical significance in SQCLC patients. A total of 156 Chinese patients diagnosed with SQCLC were selected for this study. The expression of MAC30 in all tissues was confirmed by immunohistochemical staining. Quantitative real-time PCR was performed to analyze MAC30 mRNA expression in 32 cases of SQCLC patients with corresponding non-tumor lung tissues. We observed enhanced mRNA expression of MAC30 in SQCLC as compared to control samples. Further, elevated MAC30 protein expression was strongly associated with poor tumor differentiation, TNM stage, and lymph node metastasis. In addition, we observed that patients with increased MAC30 expression demonstrated poor overall survival. Multivariate analysis explicated that increased MAC30 expression was a valuable independent predictable factor for poor tumor differentiation and short survival in SQCLC patients. Our present study suggests that MAC30 may serve as a biomarker for poor tumor differentiation and outcomes of patients with SQCLC.

Genetic Diversity of a Chinese Native Chicken Breed, Bian Chicken, Based on Twenty-nine Microsatellite Markers

Ding, Fu-Xiang,Zhang, Gen-Xi,Wang, Jin-Yu,Li, Yuan,Zhang, Li-Jun,Wei, Yue,Wang, Hui-Hua,Zhang, Li,Hou, Qi-Rui Asian Australasian Association of Animal Productio 2010 Animal Bioscience Vol.23 No.2

The level of genetic differentiation and genetic structure in a Chinese native chicken breed, Bian chicken, and two controlled chicken populations (Jinghai chicken and Youxi chicken in China) were analysed based on 29 microsatellite markers. A total of 166 distinct alleles were observed across the 3 breeds, and 32 of these alleles (19.3%) were unique to only 1 breed. Bian chicken carried the largest number of private alleles at 15 (46.9%), followed by the Jinghai chicken with 12 private alleles (37.5%). The average polymorphism information content (0.5168) and the average expected heterozygote frequency (0.5750) of the Bian chicken were the highest, and those of the Jinghai chicken were 0.4915 and 0.5505, respectively, which were the lowest. Among 29 microsatellite loci, there were 15 highly informative loci in Bian chicken, and the other 14 were reasonably informative loci. The highly informative loci in Jinghai chicken and Youxi chicken were 17 and 14 respectively. Significant deviations from the Hardy-Weinberg equilibrium were observed at several locus-breed combinations, showing a deficit of heterozygotes in many cases. As a whole, genetic differentiation among the breeds estimated by the fixation index (Fst) were at 6.7% (p<0.001). The heterozygote deficit within population (Fis) was 22.2% (p<0.001), with the highest (0.249) in Bian chicken and lowest (0.159) in Youxi chicken. These results serve as an initial step in the plan for genetic characterization and conservation of the Chinese chicken genetic resource of Bian, as well as Jinghai and Youxi chickens.

Noninvasive monitoring of mouse renal allograft rejection using micro-CT

Jiangang Hou,Masayuki Fujino,Songjie Cai,Qiang Ding,Xiao-Kang Li 대한외과학회 2015 Annals of Surgical Treatment and Research(ASRT) Vol.88 No.5

Purpose: Acute renal graft rejection can only be definitively diagnosed by renal biopsy. However, biopsies carry a risk of renal transplant injury and loss. Micro-CT is widely used in preclinical studies of small animals. Here, we propose micro-CT could noninvasively monitor and evaluate renal location and function in a mouse kidney transplant model. Methods: Orthotopic kidney transplantation was performed in a BALB/c -to- C57BL/6j or C57BL/6j-to- C57BL/6j mouse model. After optimizing imaging techniques, five mice were imaged with micro-CT and the findings were verified histologically. Results: Micro-CT can monitor and evaluate renal location and function after orthotopic kidney transplantation. There were no mice deaths while renal transplants were failure. Conclusion: We propose that graft micro-CT imaging is a new option that is noninvasive and specific, and can aid in early detection and follow-up of acute renal rejection. This method is potentially useful to improve posttransplant rejection monitoring.

STING Negatively Regulates Double-Stranded DNA-Activated JAK1-STAT1 Signaling via SHP-1/2 in B Cells

Yayi Hou,Guanjun Dong,Ming You,Liang Ding,Hongye Fan,Fei Liu,Deshan Ren 한국분자세포생물학회 2015 Molecules and cells Vol.38 No.5

Recognition of cytosolic DNA initiates a series of innate immune responses by inducing IFN-I production and subsequent triggering JAK1-STAT1 signaling which plays critical roles in the pathogenesis of infection, inflammation and autoimmune diseases through promoting B cell activation and antibody responses. The stimulator of interferon genes protein (STING) has been demonstrated to be a critical hub of type I IFN induction in cytosolic DNA-sensing pathways. However, it still remains unknown whether cytosolic DNA can directly activate the JAK1-STAT1 signaling or not. And the role of STING is also unclear in this response. In the present study, we found that dsDNA directly triggered the JAK1-STAT1 signaling by inducing phosphorylation of the Lyn kinase. Moreover, this response is not dependent on type I IFN receptors. Interestingly, STING could inhibit dsDNA-triggered activation of JAK1-STAT1 signaling by inducing SHP-1 and SHP-2 phosphorylation. In addition, compared with normal B cells, the expression of STING was significantly lower and the phosphorylation level of JAK1 was significantly higher in B cells from MRL/lpr lupus-prone mice, highlighting the close association between STING low-expression and JAK1-STAT1 signaling activation in B cells in autoimmune diseases. Our data provide a molecular insight into the novel role of STING in dsDNA-mediated inflammatory disorders.

Single-Phase Boost Type Power Factor Corrector with Embedded Active Buffer Achieving Power Decoupling

Kaicheng Ding,Yan Zhang,Jinjun Liu,Rui Cao,Yuetao Hou,Xin Meng 전력전자학회 2019 ICPE(ISPE)논문집 Vol.2019 No.5

In this paper, a boost type Power Factor Corrector is proposed to deal with the inherent issue of double line frequency power ripple, namely power coupling, in single-phase AC/DC conversion systems. The proposed converter is embedded with an active buffer circuit and the redundant capacitor and power switches enable active power decoupling and an all-film-capacitor implementation. The active power decoupling control scheme in this paper is based on the direct control of ac power and dc power and is therefore relatively easy to implement. The former one helps to achieve unit power factor at ac side while the latter one helps to regulate dc side output voltage and as a result the double line frequency power ripple could be automatically transferred onto the buffer capacitor of the converter. Theoretical analysis, simulation and experiment results are provided in the paper.

An effective route for growth of WO3/BiVO4 heterojunction thin films with enhanced photoelectrochemical performance

Chunyun Hou,Jiangwei Yu,Jin-Rui Ding,Weiqiang Fan,Hongye Bai,Dongbo Xu,Wei-dong Shi 한국공업화학회 2021 Journal of Industrial and Engineering Chemistry Vol.104 No.-

The unsatisfactory solar light absorption of WO3 and poor charge separation of BiVO4 are main limits fortheir use in photoelectrochemical (PEC) water oxidation. Coupling WO3 with BiVO4 has been consideredas a feasible way to improve PEC performance by taking complementary advantages of them. In thiswork, we obtained nanoflake-structured WO3 by hydrothermal growth with post-annealing. The effectof process variables on morphology and resultant performance were investigated. Electrodepositiongrowth was utilized to deposit BiVO4 onto WO3 forming WO3/BiVO4 heterojunction thin films. PorousBiVO4 with wormlike morphology was tightly coupled and well-distributed onto WO3 nanoflakes. Theoptimized best-performing WO3/BiVO4 photoanode exhibits higher photocurrent density than that summationof bare WO3 and BiVO4 over entire range of applied potential. This enhancement is mainly attributedto the effective charge separation at WO3/BiVO4 interface, which is confirmed throughelectrochemical impedance spectra (EIS) measurements, respectively. Our work provides a referableapproach for the growth of WO3/BiVO4 heterojunction photoanode with enhanced PEC performance.

Investigation of Microscale Laser Shock Flat Hole Clinching

Yaxuan Hou,Kexin Ding,Guoxin Lu,Chao Zheng,Zhong Ji 한국정밀공학회 2022 International Journal of Precision Engineering and Vol.23 No.9

This paper proposes an improved laser shock flat hole clinching process for joining ductile and brittle materials to obtain single-sided flat joints in microscale. A copper foil is clinched with a perforated sheet and a mechanical joining is achieved by this process. Experiments and numerical simulations are conducted for three thickness combinations of 50 μm/50 μm, 30 μm/50 μm and 20 μm/100 μm to investigate the joinability of this process. The results show that a step-by-step laser shock process with a low-energy pre-shock and a high-energy secondary shock can effectively join the copper foil and the perforated sheet. The three thickness combinations of the upper and lower sheets result in three clinching structures, namely stacked, intermediate and thinning joints. And the joint strength mainly depends on the thinner sheet in a successful joining even for the single joint or the double-joint.

Seroprevalence of Dirofilaria immitis in Cats from Liaoning Province, Northeastern China

Honglie Hou,Lili Cao,Wenzhi Ren,Dansheng Wang,He Ding,Juan You,Xinhua Yao,Hang Dong,Yanbing Guo,Shuxian Yuan,Xi-Chen Zhang,Pengtao Gong 대한기생충학ㆍ열대의학회 2017 The Korean Journal of Parasitology Vol.55 No.6

The present study was performed to investigate the seroprevalence and risk factors for Dirofilaria immitis infection in cats from Liaoning province, northeastern China. From October 2014 to September 2016, sera of 651 cats, including 364 domestic cats and 287 feral cats (332 females and 319 males) were assessed. They were tested for the presence of D. immitis antigen using SNAP Heartworm RT test kit. In this population, the average prevalence was 4.5%. Age and rearing conditions (feral or domestic) were found to be associated with the prevalence of D. immitis. The prevalence was significantly higher in feral cats compared with domestic cats (8.4% vs 1.4%, P<0.01). There was no significant difference between males and females (4.7% vs 4.2%, P>0.05), but older cats (≥3 years old) showed a statistically higher prevalence compared with younger cats (<3 years old) in feral populations (16.8 vs 2.4%, P<0.01), while the difference between the age groups was not statistically significant in domestic cats (2.4% vs 0.51%, P>0.05), all these results suggest that outdoor exposure time may be one of the most important factors for D. immitis prevalence in cats. Results reveal that D. immitis are prevalence in domestic and feral cats in northeastern China, which indicates that appropriate preventive measures should be taken to decrease the incidence of feline heartworm disease in Liaoning province, northeastern China.