Abnormal differentiation of regulatory T cells and Th17 cells induced by perinatal bisphenol A exposure in female offspring mice

You‑dan Dong,Liang Gao,Feng‑juan Wu,Ren Lin,Yuan Meng,Li‑hong Jia,Xiao‑fei Wang 대한독성 유전단백체 학회 2020 Molecular & cellular toxicology Vol.16 No.2

Background Bisphenol A (BPA) is an environmental estrogen widely exposed to human beings, and there are more studies on its reproductive toxicity, endocrine disruption and neurobehavioral disorders. Recent few studies have found that BPA has immunotoxicity, and its mechanism is not clear. Therefore, the effects of BPA on immune system have attracted extensive attention. The aim of this study was to investigate the effect and mechanism of perinatal exposure to BPA on regulatory T cells (Treg) and Th17 cells in female offspring mice. Methods Twenty-one pregnant C57BL/6 mice were randomly divided into three groups: a control group, low-dose BPA (0.2 μg/mL) and high-dose BPA (2.0 μg/mL) exposure group. All received BPA exposure via drinking water from gestational day 6 to the end of lactation. Female offspring were fed a normal diet and drinking water for 1 month. The percentages of Treg and Th17 cells, the levels of Foxp3 and RORγt protein and IL-17 and TGF-β from spleen tissue or blood were measured in female offspring. Results The percentage of Treg cells and levels of Foxp3 protein decreased, while the percentage of Th17 cells and levels of RORγt protein increased, which showed a dose–effect relationship. The levels of serum TGF-β were significantly lower and the levels of serum IL-17 were statistically higher in BPA-exposed female offspring compared with controls (P < 0.05 or P < 0.01). But there were no statistical difference in the levels of serum TGF-β and IL-17 between 0.2 μg/mL and 2.0 μg/ mL BPA groups (P > 0.05). Conclusion BPA exposure during pregnancy and lactation could cause abnormal differentiation and function of Treg and Th17 cells in female offspring mice, which was associated with down-regulated Foxp3 and up-regulated RORγt protein, respectively. Our findings indicated that BPA exposure during early development may play an important role in the development of autoimmune diseases later.

Bacillus oceani sp. nov., a new slightly halophilic bacterium, isolated from a deep sea sediment environment

Liu, Yu-Juan,Long, Li-Juan,Huang, Xiao-Fang,You, Zhi-Qing,Wang, Fa-Zuo,Li, Jie,Kim, Chang-Jin,Tian, Xin-Peng,Zhang, Si Springer-Verlag 2013 Antonie van Leeuwenhoek Vol.104 No.5

Synthesis, Characterization and pH-Responsive Self-Assembly Behavior of Amphiphilic Multiarm Star Triblock Copolymers Based on PCL, PDEAEMA, and PEG

You Qiang Yang,Wen Jing Lin,Li Juan Zhang,Cheng Zhi Cai,Wei Jiang,Xin Dong Guo,Yu Qian 한국고분자학회 2013 Macromolecular Research Vol.21 No.9

A series of amphiphilic 4- and 6-armed star triblock copolymers based on poly(ε-caprolactone) (PCL),poly(2-(diethylamino)ethyl methacrylate) (PDEAEMA), and poly(poly(ethylene glycol) methyl ether methacrylate)(PPEGMA) were designed and synthesized by a combination of ring opening polymerization (ROP) and continuous activators regenerated by electron transfer atom transfer radical polymerization (ARGET ATRP). The continuous ARGET ATRP of DEAEMA and PEGMA was in situ monitored by react infrared spectroscopy (ReactIR) and showed good first-order kinetic characteristics. The molecular weights and chemical structures of the copolymers and their precursors were confirmed by gel permeation chromatography (GPC) and 1H NMR. The critical micelle concentration (CMC) values of the star copolymers in aqueous solution were extremely low (2.2-4.0 mg/L), depending on the architecture of the copolymers. The pH-responsive self-assembly behavior of the star copolymers in aqueous solution was investigated by a combination of dynamic light scattering (DLS), UV-vis spectrometry and scanning electron microscopy (SEM). When the pH values decreased from 10 to 3, no obvious fluctuation of the visible light transmittance of the micelle solutions was observed for lower polymer concentrations of 0.1 and 1 mg/mL, while sharp increase occurred at higher concentration of 10 mg/mL. The hydrodynamic diameters (Dh) of the micelle solutions appeared slight increase with the increase of concentration, and increased rapidly as the pH decreased from 10 to 4 followed by a slight decrease at pH 3. The effects of pH value on the zeta potentials exhibited almost the same tendency with the Dh. This may due to the fact that the protonation of tertiary amine groups in DEAEMA can induce the swelling of micelles. The PCL and PDEAEMA contents and the topological structures (4-or 6-arm) showed significant influences on the pH-sensitivity of the micelles. Overall, the results demonstrated that the structures and pH-sensitivity of these amphiphilic copolymers could be well-controlled and their self-assembled micelles are promising carriers for delivery of anticancer hydrophobic drugs.

Mold Filling Analysis of an Alignment Structure in Micro Hot Embossing

Gomez, Juan A.,Conner, Glenn T.,Chun, Du Hwan,Kim, Yoo-Jae,Song, In-Hyouk,You, Byoung Hee THE KOREAN FIBER SOCIETY 2014 FIBERS AND POLYMERS Vol.15 No.6

Hot embossing is one of the most popular fabrication methods to replicate polymer microdevices in the field of micro-fluidics and micro-optics. Numerical models for hot embossing were constructed to analyze the advance of flow front of the molten polymer using commercial software, DEFORM-2D. A hemisphere tipped post, used as an alignment structure in the assembly of micro devices, was modeled to demonstrate the flow behavior of the molten polymer in mold filling. Hot embossing experiments were performed to validate the feasibility of the numerical models. Most of the simulations showed agreement with experiments. The mold filling was estimated with the heights of the embossed posts in the analysis. No significant mold filling with the molten polymer was observed below the glass transition temperature of $105^{\circ}C$. The mold cavity was completely filled with the polymer at the molding temperature of $137.5^{\circ}C$ and $150^{\circ}C$ while the embossing forces were 300, 600, and 900 N.

An Iterative Algorithm for Solving the Least-Squares Problem of Matrix Equation AXB+CYD=E

Kai-juan Shen,Chuan-hua You,Yu-xia Du 한국전산응용수학회 2008 Journal of applied mathematics & informatics Vol.26 No.5

In this paper, an iterative method is proposed to solve the least-squares problem of matrix equation AXB + CY D = E over un-known matrix pair [X, Y ]. By this iterative method, for any initial matrix pair [X1, Y1], a solution pair or the least-norm least-squares solution pair of which can be obtained within finite iterative steps in the absence of roundoff errors. In addition, we also consider the optimal approximation problem for the given matrix pair [X0, Y0] in Frobenius norm. Given numerical examples show that the algorithm is efficient. In this paper, an iterative method is proposed to solve the least-squares problem of matrix equation AXB + CY D = E over un-known matrix pair [X, Y ]. By this iterative method, for any initial matrix pair [X1, Y1], a solution pair or the least-norm least-squares solution pair of which can be obtained within finite iterative steps in the absence of roundoff errors. In addition, we also consider the optimal approximation problem for the given matrix pair [X0, Y0] in Frobenius norm. Given numerical examples show that the algorithm is efficient.

Enhancement of pool boiling heat transfer in water on aluminum surface with high temperature conductive microporous coating

Godinez, Juan C.,Fadda, Dani,Lee, Jungho,You, Seung M. Elsevier 2019 INTERNATIONAL JOURNAL OF HEAT AND MASS TRANSFER - Vol.132 No.-

<P><B>Abstract</B></P> <P>The effects of an aluminum high temperature conductive microporous coating (Al-HTCMC) on the nucleate boiling heat transfer (NBHT) coefficient and critical heat flux (CHF) are studied in saturated distilled water at 1 atm. Aluminum powders with three different mean particle diameters (d<SUB>m</SUB> = 11, 24, and 66 µm) are used in the fabrication of the Al-HTCMC. For each mean particle diameter, an optimal coating thickness to yield the highest NBHT coefficient is determined. The optimized Al-HTCMC thickness is found to result in comparable NBHT coefficients regardless of the particle diameter. Pool boiling tests with a plain aluminum surface are used for comparison. The coated and plain aluminum surfaces are treated equally before the pool boiling tests to establish a Boehmite oxidation nano layer on the aluminum surfaces. Following the Boehmite treatment, the contact angle is unmeasurable (∼0°) with the Al-HTCMC surface and 12° with a plain aluminum surface. Then, pool boiling tests are performed and reveal comparable CHF (1725–1850 kW/m<SUP>2</SUP>) values with or without the Al-HTCMC. However, the Al-HTCMC is shown experimentally to improve the NBHT coefficient by a factor of five as the wall superheat is reduced by from 31 K to 6 K just before CHF. The results obtained are also compared to similar work using an HTCMC layer on a copper surface to demonstrate the performance of the Al-HTCMC.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Study of the effects of an aluminum high temperature conductive microporous coating on pool boiling. </LI> <LI> Aluminum microporous coating (Al-HTCMC) fabricated using different aluminum powder particle sizes. </LI> <LI> Coating thickness optimized to yield highest nucleate boiling heat transfer coefficients. </LI> <LI> Boehmite treatment applied to aluminum surface with the microporous coating. </LI> </UL> </P>

Aromatic Diamino-bridged Bis(β-cyclodextrin) as Fluorescent Sensor for the Molecular Recognition of Bile Salts

Yan Zhao,Juan Gu,Shao Ming,Shao Ming Chi,Yong Cun Yang,Yu Fei Wang,Hong You Zhu,Jian Hong Liu,Rong Huang 대한화학회 2008 Bulletin of the Korean Chemical Society Vol.29 No.11

representative bile salts, i.e., cholate (CA), deoxycholate (DCA), glycocholate (GCA) and taurocholate (TCA), has been investigated at 25 °C in phosphate buffer (pH 7.20) by fluorescence, circular dichroism and 2D NMR spectroscopy. The result indicated that the bis(β-cyclodextrin) 2 acts as fluorescent sensor and displays remarkable fluorescence enhancement upon addition of optically inert bile salts. Form the induced circular dichroism (ICD) and ROESY spectra, it is deduced that the phenyl moiety in the linker of bis(β-cyclodextrin) 2 is partially self-included in the CD cavity, and is not expelled out of the CD cavity upon complexation with bile guests. Owing to the cooperative host-tether-guest binding mode in which the linker and guest are coincluded in the two CD cavities, bis(β-cyclodextrin) 2 significantly enhanced binding ability and molecular selectivity as compared with the native β-cyclodextrin 1 through the simultaneous contributions of hydrophobic, hydrogen bond, and electrostatic interactions. The complex stability constants are discussed comparatively and globally from the viewpoints of multiple recognition between host and guest.

AN ITERATIVE ALGORITHM FOR SOLVING THE LEAST-SQUARES PROBLEM OF MATRIX EQUATION AXB+CYD=E

Shen, Kai-Juan,You, Chuan-Hua,Du, Yu-Xia Korean Society of Computational and Applied Mathem 2008 Journal of applied mathematics & informatics Vol.26 No.5

In this paper, an iterative method is proposed to solve the least-squares problem of matrix equation AXB+CYD=E over unknown matrix pair [X, Y]. By this iterative method, for any initial matrix pair [$X_1,\;Y_1$], a solution pair or the least-norm least-squares solution pair of which can be obtained within finite iterative steps in the absence of roundoff errors. In addition, we also consider the optimal approximation problem for the given matrix pair [$X_0,\;Y_0$] in Frobenius norm. Given numerical examples show that the algorithm is efficient.

Preparation and properties of the green-emitting phosphors NaCa0.98-xMgxPO4:

Bo Yue,Juan Gu,Guangfu Yin,Zhongbing Huang,Xiaoming Liao,Yadong Yao,Yunqing Kang,Panli You 한국물리학회 2010 Current Applied Physics Vol.10 No.4

In this study, a series of the green-emitting phosphors NaCa0.98-xMgxPO4:Eu2þ 0:02 was prepared through high temperature solid-state reaction. The structure and spectroscopic properties of these samples were characterized by XRD, FT-IR, reflectance spectra and photoluminescence (PL) spectra, respectively. The effect of Mg2+ co-doping concentration on the PL intensity of as-prepared samples was also investigated. The enhanced intensities of excitation and emission band of Eu2+ and a slight red-shift of the emission peak were observed due to Mg2+ co-doping. When the x value is 0.08, the phosphors possessed the maximum emission intensity monitored at 360 nm and the emission spectrum shows a single band centered at about 500 nm. The obtained phosphors had strong absorption in near-UV zone (350–400 nm), which can match the emission of near-UV light-emitting diodes. The prepared material is potential green phosphors for white LEDs.

A phase I dose-finding trial of hyperthermic intraperitoneal docetaxel combined with cisplatin in patients with advanced-stage ovarian cancer

Zhi-yao You,Hui Li,Miao-fang Wu,Yanfang Ye,Li-juan Wang,Zhongqiu Lin,Jing Li 대한부인종양학회 2024 Journal of Gynecologic Oncology Vol.35 No.1

Objective: To identif y the maximum tolerated dose (MTD) of docetaxel combined with afixed dose of cisplatin (75 mg/m2) delivered as hyperthermic intraperitoneal chemotherapy(HIPEC) in patients with ovarian cancer. Methods: In this phase I trial, a time-to-event Bayesian optimal inter val design was used. Docetaxel was given at a starting dose of 60 mg/m2 and was increased in 5 mg/m2 incrementsuntil the MTD was determined or the maximum dose level of 75 mg/m2 was reached. The dose-limiting toxicity (DLT) rate was set at 25%, with a total sample size of 30 patients. HIPEC wasdelivered immediately following debulking surger y at a target temperature of 43°C for 90 minutes. Results: From August 2022 to November 2022, 30 patients were enrolled. Among thepatients who received a dose of docetaxel ≤65 mg/m2, no DLT was reported. DLTs wereobser ved in one patient who received 70 mg/m2 docetaxel (grade 3 anaemia) and in threepatients who received 75 mg/m2 docetaxel (one case of grade 3 anaemia, one case of grade3 hepatic impairment and one case of grade 4 thrombocytopenia). Patients treated withdocetaxel 75 mg/m2 in combination with cisplatin 75 mg/m2 had an estimated DLT rate of25%, which was the closest to the target DLT rate and was therefore chosen as the MTD. Conclusion: Docetaxel, in combination with a fixed dose of cisplatin (75 mg/m2), can be usedsafely at intraperitoneal doses of 75 mg/m2 in ovarian cancer patients who received HIPEC(43°C, 90 minutes) following debulking surger y. Trial Registration: ClinicalTrials.gov Identifier: NCT05410483