Plasma metabolites associated with physiological and biochemical indexes indicate the effect of caging stress on mallard ducks (Anas platyrhynchos)

Zheng Chao,Wu Yan,Liang Zhen Hua,Pi Jin Song,Cheng Shi Bin,Wei Wen Zhuo,Liu Jing Bo,Lu Li Zhi,Zhang Hao 아세아·태평양축산학회 2022 Animal Bioscience Vol.35 No.2

Objective: Cage rearing has critical implications for the laying duck industry because it is convenient for feeding and management. However, caging stress is a type of chronic stress that induces maladaptation. Environmental stress responses have been extensively studied, but no detailed information is available about the comprehensive changes in plasma metabolites at different stages of caging stress in ducks. We designed this experiment to analyze the effects of caging stress on performance parameters and oxidative stress indexes in ducks. Methods: Liquid chromatography tandem mass spectrometry (LC/MS-MS) was used to determine the changes in metabolites in duck plasma at 5 (CR5), 10 (CR10), and 15 (CR15) days after cage rearing and traditional breeding (TB). The associated pathways of differentially altered metabolites were analyzed using Kyoto encyclopedia of genes and genomes (KEGG) database. Results: The results of this study indicate that caging stress decreased performance parameters, and the plasma total superoxide dismutase levels were increased in the CR10 group compared with the other groups. In addition, 1,431 metabolites were detected. Compared with the TB group, 134, 381, and 190 differentially produced metabolites were identified in the CR5, CR10, and CR15 groups, respectively. The results of principal component analysis (PCA) show that the selected components sufficiently distinguish the TB group and CR10 group. KEGG analysis results revealed that the differentially altered metabolites in duck plasma from the CR5 and TB groups were mainly associated with ovarian steroidogenesis, biosynthesis of unsaturated fatty acids, and phenylalanine metabolism. Conclusion: In this study, the production performance, blood indexes, number of metabolites and PCA were compared to determine effect of the caging stress stage on ducks. We inferred from the experimental results that caging-stressed ducks were in the sensitive phase in the first 5 days after caging, caging for approximately 10 days was an important transition phase, and then the duck continually adapted. Objective: Cage rearing has critical implications for the laying duck industry because it is convenient for feeding and management. However, caging stress is a type of chronic stress that induces maladaptation. Environmental stress responses have been extensively studied, but no detailed information is available about the comprehensive changes in plasma metabolites at different stages of caging stress in ducks. We designed this experiment to analyze the effects of caging stress on performance parameters and oxidative stress indexes in ducks.Methods: Liquid chromatography tandem mass spectrometry (LC/MS-MS) was used to determine the changes in metabolites in duck plasma at 5 (CR5), 10 (CR10), and 15 (CR15) days after cage rearing and traditional breeding (TB). The associated pathways of differentially altered metabolites were analyzed using Kyoto encyclopedia of genes and genomes (KEGG) database.Results: The results of this study indicate that caging stress decreased performance parameters, and the plasma total superoxide dismutase levels were increased in the CR10 group compared with the other groups. In addition, 1,431 metabolites were detected. Compared with the TB group, 134, 381, and 190 differentially produced metabolites were identified in the CR5, CR10, and CR15 groups, respectively. The results of principal component analysis (PCA) show that the selected components sufficiently distinguish the TB group and CR10 group. KEGG analysis results revealed that the differentially altered metabolites in duck plasma from the CR5 and TB groups were mainly associated with ovarian steroidogenesis, biosynthesis of unsaturated fatty acids, and phenylalanine metabolism.Conclusion: In this study, the production performance, blood indexes, number of metabolites and PCA were compared to determine effect of the caging stress stage on ducks. We inferred from the experimental results that caging-stressed ducks were in the sensitive phase in the first 5 days after caging, caging for approximately 10 days was an important transition phase, and then the duck continually adapted.

Circ-SNX27 sponging miR-375/RPN1 axis contributes to hepatocellular carcinoma progression

Chao Zheng,Jin Liang,Shoude Yu,Hua Xu,Lin Dai,Dan Xu The Korean Society of Pharmacology 2023 The Korean Journal of Physiology & Pharmacology Vol.27 No.4

Hepatocellular carcinoma (HCC) is a prevalent malignant tumor with high fatality. It has yet to be reported whether circ-SNX27 can affect the progression of HCC. This study attempted to analyze circ-SNX27's precise role and underlying mechanisms in HCC. HCC cell lines and tumor specimens from HCC patients were analyzed using quantitative real-time PCR and Western blotting to quantify the expressions of circ-SNX27, miR-375, and ribophorin I (RPN1). Cell invasion and cell counting kit 8 experiments were conducted for the evaluation of HCC cell invasion and proliferation. Caspase-3 Activity Assay Kit was utilized to gauge the caspase-3 activity. Luciferase reporter and RNA immunoprecipitation assays were executed to ascertain the relationships among miR-375, circ-SNX27, and RPN1. To determine how circ-SNX27 knockdown affects the growth of HCC xenografts in vivo, tumor-bearing mouse models were constructed. Elevated expressions of circ-SNX27 and RPN1 as well as a reduced miR-375 expression were observed among HCC cells and HCC patient tumor specimens. Knocking-down circ-SNX27 in HCC cells abated their proliferative and invasive abilities but raised their caspase-3 activity. Moreover, the poor levels of circ-SNX27 inhibited HCC tumor growth among the mice. Circ-SNX27 enhanced RPN1 by competitively binding with miR-375. Silencing miR-375 in HCC cells promoted their malignant phenotypes. Nonetheless, the promotive effect of miR375 silencing was reversible via the knockdown of circ-SNX27 or RPN1. This research demonstrated that circ-SNX27 accelerated the progression of HCC by modulating the miR-375/RPN1 axis. This is indicative of circ-SNX27's potential as a target for the treatment of HCC.

Research on Fracture of Aluminum Foil in Microscale Laser Peen Forming

Chao Zheng,Sheng Sun,Jing Liu,Zhong Ji 한국소성가공학회 2010 기타자료 Vol.2010 No.6

A novel numerical method for dynamic fracture in microscale laser peen forming (μLPF) of aluminum foils was presented and the role of the die diameter on fracture behavior at the ultra high strain rate was investigated via both experimental and numerical methods. μLPF is a process in which the plastic deformation is generated through laserinduced shock wave and compressive residual stresses can be imparted to improve the fatigue life of micro parts. During μLPF, the pressure exerted on the target is higher than 1 GPa and the strain rate is greater than 10? s<SUP>-1</SUP>, so the mechanical behavior of materials in this dynamic process is very different from that under static or quasi-static conditions. In the present study, the finite element method with grain and grain boundary elements was used to analyze the μLPF process of aluminum foils with a thickness of 60 μm. The onset and propagation of crack were simulated in this way that the specified nodes were tied together until the equivalent plastic strain exceeded a certain value. Under a given value of plastic strain, the influence of die diameters of 0.6, 1.0, and 1.6 mm on the fracture mode of the material was predicted. A series of experiments were carried out to verify the numerical model. The geometrical morphologies of fracture regions were observed via optical microscope and scan electron microscope. In results from both experiments and simulations, the size of the die diameter affects the location of the fracture: (I) Fracture appeared at the entrance of the die for die diameters of 0.6 and 1.0 mm. (II) Fracture occurred near the centre of the formed dome for 1.6 mm die diameter. The generation mechanism of two fracture modes was explained. This work provides a preliminary insight into the fracture behavior of materials under the ultra high strain rate and lays the ground work for more in-depth simulations in the future study.

A novel design method for improving collapse resistances of multi-story steel frames with unequal spans using steel braces

Zheng Tan,Wei-hui Zhong,Bao Meng,Shi-chao Duan,Hong-chen Wang3,Xing-You Yao,Yu-hui Zheng 국제구조공학회 2023 Steel and Composite Structures, An International J Vol.47 No.2

The bearing capacities resisted by the two-bay beams of multi-story planar frames with unequal spans under column removal scenarios differ considerably owing to the asymmetric stress on the left and right beams connected to the failed column and cause the potential for beams with larger span-to-depth ratios to be unable to exert effectively, which is disadvantageous for resisting the vertical load in unequal-span frame structures. To address this problem, the structural measure of adding braces to the weak bays of multi-story unequal-span frames was proposed, with the objective of achieving a coordinated stress state in two-bay beams with unequal spans, thereby improving the collapse resistance of unequal-span frame structures. Before conducting the numerical simulation, the modeling methods were verified by previous experimental results of two multi-story planar frames with and without steel braces. Thereafter, the effects of the tensile and compressive braces on the collapse behavior of the frame structures were elucidated. Then, based on the mechanical action laws of the braces throughout the collapse process, a detailed design method for improving the collapse resistance of unequal-span frame structures was proposed. Finally, the proposed design method was verified by using sufficient example models, and the results demonstrated that the design method has good application prospects and high practical value.

Influence of heat treatment on the wear life of hydraulic fracturing tools

Chao Zheng,Yonghong Liu,Hanxiang Wang,Jie Qin,Yang Shen,Shihong Zhang 대한기계학회 2017 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.31 No.2

Wear phenomenon has caused severe damage or failure of fracturing tools in oil and gas industry. In this paper, influence of heat treatment on the mechanical properties and wear resistance of fracturing tool made of lamellar graphite grey cast iron were investigated. The surface composition and microstructure were characterized by X-ray diffraction (XRD) and metallographic microscope. Sliding wear tests were performed to study the tribological behavior. Tests results showed that wear rates of treated specimens decreased by 33 %. Besides, worn morphology and wear debris were analyzed using Scanning electron microscope (SEM) and Energy dispersive Xray spectra (EDS). Wear failure mechanisms of specimens were identified. Furthermore, on-site experiment results indicated that wear loss of treated samples decreased by 37.5 %. The wear life of hydraulic fracturing tools can be improved obviously by the heat treatment.

Experimental study on the wear behavior of HVOF sprayed nickel-based coating

Chao Zheng,Yonghong Liu,Jie Qin,Renjie Ji,Shihong Zhang 대한기계학회 2018 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.32 No.1

Severe wear is a hot issue in the development of petroleum engineering which has attracted many attentions. To address this problem, a nickel-based alloy (NiCrBSiWFeCoC) coating was proposed and prepared using High-velocity oxy-fuel (HVOF) technique. The wear properties of as-sprayed composite coating were studied. The sliding wear experiments of both as-sprayed and uncoated specimens were conducted on a tribometer. The obtained results indicated that the both wear rates increased obviously with the increase in normal load, however, the friction coefficients decreased with the increase in normal load. Based on the comprehensive analyses, it can be concluded that the as-sprayed coating exhibited better wear resistance. Furthermore, the wear behaviors can be investigated by analyzing the worn morphologies. This research can provide insight into the wear prevention in petroleum engineering and decreases the failure of equipments or pipelines.

Study on the Influence of UHVDC Dynamic Response on Recovery Characteristic of Receiving End Power Grid

Zheng, Chao,Zhang, Kai,Sheng, Canhui,Lin, Junjie,Xue, Jinying,Chen, Dezhi,Zhang, Zhiqiang,Luo, Bangyun 대한전기학회 2014 The Journal of International Council on Electrical Vol.4 No.1

Aiming at the three UHVDC controller model in the electromechanical simulation software BPA which widely used in China, the structural features were analyzed. By the time-domain simulation, the influence of different simulation models and the key controller parameters on the recovery characteristics of the receiving end power system were studied. Simulation results show that the commutation failure simulated method impacts the recovery characteristic significantly for weak power grids, increasing the voltage measurement time constant of constant dc power control module and enhancing the start voltage threshold of voltage dependent current order limit (VDCOL) can decrease the reactive power consumption of inverter during the disturbance, and can help to improve the recovery characteristic of receiving end power grid.

Adaptive integral sliding mode control with bounded <b><i>ℒ</i></b>₂ gain performance of uncertain quantised control systems

Bo-Chao Zheng,Park, Ju H. IET 2015 IET CONTROL THEORY AND APPLICATIONS Vol.9 No.15

<P>Adaptive integral sliding mode control (ISMC) is investigated for a class of non-linear uncertain systems considering the mismatch relation between quantisation sensitivity parameters. First, by analysing the operation principle of the encoder/decoder, a mismatched relation between the quantisation parameters is established by using a time-varying ratio model, where the boundaries of the time-varying ratio can be unknown, regarding the existing cases as special ones. Second, using linear matrix inequality techniques, the prescribed bounded L-2 gain performance condition is obtained for the sliding dynamics. Sequently, based on the formed boundary unknown time-varying ratio relation, adaptive ISMC laws are designed to ensure the reachability of the sliding manifold, then the establishment of the stability of the closed-loop system. Finally, an example is provided to illustrate the effectiveness of the proposed methods.</P>

A Sliding Sector Approach to Quantized Feedback Variable Structure Control

Bo-Chao Zheng,Yan-Mei Xue 제어·로봇·시스템학회 2013 International Journal of Control, Automation, and Vol.11 No.6

This paper is concerned with the quantized feedback quadratic stabilization problem for linear time-invariant systems. Sliding sector based quantized state feedback variable structure control schemes are established. The main benefit of the sliding sector technique is that it can avoid chattering caused by the utilization of variable structure control strategy. With the proposed discrete on-line adjustment of the quantization parameter, it is shown that the proposed sliding sector based sliding mode controllers can tackle state quantization and guarantee quadratic stability of the closed-loop system. Simulation results are given to verify the effectiveness of the proposed method.

Event-triggered Sliding Mode Fault-tolerant Consensus for a Class of Leader-follower Multi-agent Systems

Bo-Chao Zheng,Lina Guo,Kun Li 제어·로봇·시스템학회 2021 International Journal of Control, Automation, and Vol.19 No.8

This paper concerns with the robust sliding mode fault-tolerant consensus problem for a class of undertain second-order leader-follower multi-agent systems based on event-triggering strategies. First, a sliding mode faulttolerant controller which uses the bound information of actuator failure rate and the event-triggering threshold is designed to ensure the robust consensus of the multi-agent systems, and the range of the sliding mode band is also shown. Second, by constructing an equivalent relationship, the upper bound of robust consensus error is alsogiven. Third, the minimum event-triggered execution time for the second-order leader-follower multi-agent systems is calculated. Finally, the simulation results verify the effectiveness of the proposed event-triggered sliding mode fault-tolerant consensus algorithm.