Recommendations on management of gynecological malignancies during the COVID-19 pandemic: perspectives from Chinese gynecological oncologists

Yingmei Wang,Shiqian Zhang,Lihui Wei,Zhongqiu Lin,Xinyu Wang,Jianliu Wang,Keqin Hua,Manhua Cu,Jiandong Wang,Shixuan Wang,Wen Di,Yudong Wang,Ruifang An,Mingrong Xi,Ruixia Guo,Qi Zhou,Xing Xie,Fengxia X 대한부인종양학회 2020 Journal of Gynecologic Oncology Vol.31 No.4

The outbreak of coronavirus disease 2019 (COVID-19) caused by the severe acute respiratorysyndrome coronavirus 2 has rapidly spread globally. Cancer patients are at a higher risk ofbeing infected with the coronavirus and are more likely to develop severe complications, ascompared to the general population. The increasing spread of COVID-19 presents challengesfor the clinical care of patients with gynecological malignancies. Concerted efforts should beput into managing gynecological malignancies in an orderly manner by strictly implementingthe measures that are specifically developed for controlling the spread of COVID-19. We havedrafted Recommendations on Management of Gynecological Malignancies during the COVID-19 Pandemicbased on our experience on controlling COVID-19 pandemic in China. We recommendthat patients with gynecological malignancies should be managed in hierarchical andindividualized manners in combination with local conditions related to COVID-19. Medicalcare decision should be balanced between controlling COVID-19 pandemic spread and timelydiagnosis and treatment for gynecologic oncology patients.

HIGH-ORDERED AND ULTRA-SENSITIVE PARTICLE-IN-BOWL METALLIC ARRAYS FOR SURFACE ENHANCED RAMAN SPECTROSCOPY

DI DI,PEITAO DONG,XUEZHONG WU,CHAOGUANG WANG,JIAN CHEN,HAOXU WANG,SHENGYI LI,JUNFENG WANG 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2014 NANO Vol.9 No.4

High-ordered particle-in-bowl (PIB) arrays are developed in this paper for surface enhancedRaman spectroscopy (SERS). A heterogeneous shadow mask, composing of the chrome (Cr) layerand colloid residues, is used to fabricate the silicon (Si) template from where the PIB arrays¯nally lift-o®. The ¯nite di®erence time domain (FDTD) method is employed to investigate theRaman enhancement mechanism of this PIB architecture. The electromagnetic (EM) ¯eld tendsto concentrate in the gap between the bowl and the particle forming the \hot spots". Theenhancement factor (EF) of the EM ¯eld is about 70 with an excitation wavelength of 785 nm. The Raman measurements validate the EM calculation of the PIB arrays. The EF is about1 : 12 ? 10 7 using Rodamine 6G (R6G) as probe molecule. The proposed PIB array is high-orderedin morphology and ultra-sensitive in Raman measurement, providing an ideal substrate forSERS-based bio-chemical sensing, disease diagnosis and analytical chemistry.

SIRT5-related desuccinylation modification of AIFM1 protects against compression-induced intervertebral disc degeneration by regulating mitochondrial homeostasis

Mao Jianxin,Wang Di,Wang Dong,Wu Qi,Shang Qiliang,Gao Chu,Wang Huanbo,Wang Han,Du Mu,Peng Pandi,Jia Haoruo,Xu Xiaolong,Wang Jie,Yang Liu,Luo Zhuojing 생화학분자생물학회 2023 Experimental and molecular medicine Vol.55 No.-

Mitochondrial dysfunction plays a major role in the development of intervertebral disc degeneration (IDD). Sirtuin 5 (SIRT5) participates in the maintenance of mitochondrial homeostasis through its desuccinylase activity. However, it is still unclear whether succinylation or SIRT5 is involved in the impairment of mitochondria and development of IDD induced by excessive mechanical stress. Our 4D label-free quantitative proteomic results showed decreased expression of the desuccinylase SIRT5 in rat nucleus pulposus (NP) tissues under mechanical loading. Overexpression of Sirt5 effectively alleviated, whereas knockdown of Sirt5 aggravated, the apoptosis and dysfunction of NP cells under mechanical stress, consistent with the more severe IDD phenotype of Sirt5 KO mice than wild-type mice that underwent lumbar spine instability (LSI) surgery. Moreover, immunoprecipitation-coupled mass spectrometry (IP-MS) results suggested that AIFM1 was a downstream target of SIRT5, which was verified by a Co-IP assay. We further demonstrated that reduced SIRT5 expression resulted in the increased succinylation of AIFM1, which in turn abolished the interaction between AIFM1 and CHCHD4 and thus led to the reduced electron transfer chain (ETC) complex subunits in NP cells. Reduced ETC complex subunits resulted in mitochondrial dysfunction and the subsequent occurrence of IDD under mechanical stress. Finally, we validated the efficacy of treatments targeting disrupted mitochondrial protein importation by upregulating SIRT5 expression or methylene blue (MB) administration in the compression-induced rat IDD model. In conclusion, our study provides new insights into the occurrence and development of IDD and offers promising therapeutic approaches for IDD.

Green-revertible Chlorina 1 (grc1) is Required for the Biosynthesis of Chlorophyll and the Early Development of Chloroplasts in Rice

Jieqin Li,Yihua Wang,Juntao Chai,Lihua Wang,Chunming Wang,Wuhua Long,Di Wang,Yunlong Wang,Ming Zheng,Cheng Peng,Mei Niu,Jianmin Wan 한국식물학회 2013 Journal of Plant Biology Vol.56 No.5

The nuclear genes involved in chloroplast developmentand chlorophyll biosynthesis must be investigated tounderstand their functions in plant growth and development. In this study, we isolated and identified a unique leaf-colormutant of rice with a green-yellow phenotype before thefour-leaf stage and named the mutation green-revertiblechlorina 1 (grc1). The mutants had significantly lower plantheight, number of tillers, and panicle length and headedsignificantly earlier than the wild type. The levels ofchlorophylls, carotenoids, and chlorophyll precursors werealso lower. The mutation in grc1 affected chloroplastultrastructure, particularly thylakoid development. Geneticanalysis indicated that the green-yellow phenotype wascontrolled by a single recessive gene. We mapped the grc1gene to a 32.4-kb region on the long arm of chromosome 6. Through map-based cloning, we identified a 45-bp insertionin the genomic region of LOC_Os06g40080, which encodeda heme oxygenase. Expression of LOC_Os06g40080 wassignificantly down-regulated in the grc1 mutant. Subcellularlocalization showed that this heme oxygenase was localizedin the chloroplast. In summary, we isolated and identified thegene for grc1, which plays an important role in chlorophyllbiosynthesis and chloroplast development in rice.

A putative plastidial adenine nucleotide transporter, BRITTLE1-3, plays an essential role in regulating chloroplast development in rice (Oryza sativa L.)

Jia Lyu,Yihua Wang,Linglong Liu,Chunming Wang,Yulong Ren,Cheng Peng,Feng Liu,Yunlong Wang,Mei Niu,Di Wang,Ming Zheng,Kunneng Zhou,Shaolu Zhao,Fuqing Wu,Haiyang Wang,Jianmin Wan 한국식물학회 2017 Journal of Plant Biology Vol.60 No.5

Differentiation from proplastids into chloroplasts isa light- and energy-dependent process. How this process isregulated is still poorly understood at the molecular level. We herein report a new putative plastidial adenine nucleotidetransporter, BRITTLE1-3 (referred to as OsBT1-3), encoded bythe rice (Oryza sativa) White Stripe Leaf 2 (WSL2) gene. Loss of OsBT1-3 function results in defective chloroplastbiogenesis, severely reduced photosynthetic efficiency, andfinally a white stripe leaf phenotype in the first four leaves. The expression levels of genes related to chlorophyllbiosynthesis and photosynthesis are drastically reduced,accompanied with over accumulation of reactive oxygenspecies (ROS) in the wsl2 mutant. OsBT1-3 is targeted tothe chloroplasts and it expresses in almost all tissues inplants, especially in young leaves. OsBT1-3 consists of 419amino acids and exhibits features of all mitochondrialcarrier proteins, including a typical transmembrane-spanningdomain and a highly conserved sequence motif designatedas the ‘mitochondrial energy transfer signatures’. Phylogeneticanalysis shows that OsBT1-3 is a putative plastidialadenine nucleotide transporter and is most closely relatedto ZmBT1-2. Together, these observations suggest that thenew putative adenine nucleotide transporter, OsBT1-3,plays an essential role in regulating chloroplast biogenesisand maintenance of ROS homeostasis during rice seedlingde-etiolation.

Parkinson’s Disease with Fatigue: Clinical Characteristics and Potential Mechanisms Relevant to α-Synuclein Oligomer

Li-Jun Zuo,Shu-Yang Yu,Fang Wang,Yanghui Xia,Ying-Shan Piao,Yang Du,Teng-Hong Lian,Rui-Dan Wang,Qiu-Jin Yu,Ya-Jie Wang,Xiao-Min Wang,Piu Chan,Sheng-Di Chen,Yongjun Wang,Wei Zhang 대한신경과학회 2016 Journal of Clinical Neurology Vol.12 No.2

Background and Purpose The aim of this study was to identify the clinical characteristics and potential mechanisms relevant to pathological proteins in Parkinson’s disease (PD) patients who experience fatigue. Methods PD patients (n=102) were evaluated using a fatigue severity scale and scales for motor and nonmotor symptoms. The levels of three pathological proteins—α-synuclein oligomer, β-amyloid (Aβ)1-42, and tau—were measured in 102 cerebrospinal fluid (CSF) samples from these PD patients. Linear regression analyses were performed between fatigue score and the CSF levels of the above-listed pathological proteins in PD patients. Results The frequency of fatigue in the PD patients was 62.75%. The fatigue group had worse motor symptoms and anxiety, depression, and autonomic dysfunction. The CSF level of α-synuclein oligomer was higher and that of Aβ1-42 was lower in the fatigue group than in the non-fatigue group. In multiple linear regression analyses, fatigue severity was significantly and positively correlated with the α-synuclein oligomer level in the CSF of PD patients, after adjusting for confounders. Conclusions PD patients experience a high frequency of fatigue. PD patients with fatigue have worse motor and part nonmotor symptoms. Fatigue in PD patients is associated with an increased α-synuclein oligomer level in the CSF

Fabrication of nonlinear optical films based on methacrylate/silica hybrid matrix

Di Wang,Xiaodong Chen,Xingwen Zhang,Wunjun Wang,Yunlong Liu,Lijiang Hua 한국물리학회 2009 Current Applied Physics Vol.9 No.3

A free-radical polymerization was performed to fabricate nonlinear optical (NLO) hybrid films based on nanostructural building blocks and an interpenetrating methacrylate/silica hybrid matrix (IHM). A bulky chromophore (disperse orange 3) bonded with ally glycidyl ether (AGE) was incorporated into the hybrid matrix in combination with (γ-methacrylpropyl)-silsesquioxane through the polymerization. Different amounts of polyhedral oligomeric silsesquioxane as modifiers were added into the IHM to enhance properties. It has been shown that the IHM increases the crosslink density of the network and the interaction potential between dipoles to help maintain the orientational order, and the generated NLO materials enhance the Tg, nonresonant second-order nonlinearity and temporal stability. A free-radical polymerization was performed to fabricate nonlinear optical (NLO) hybrid films based on nanostructural building blocks and an interpenetrating methacrylate/silica hybrid matrix (IHM). A bulky chromophore (disperse orange 3) bonded with ally glycidyl ether (AGE) was incorporated into the hybrid matrix in combination with (γ-methacrylpropyl)-silsesquioxane through the polymerization. Different amounts of polyhedral oligomeric silsesquioxane as modifiers were added into the IHM to enhance properties. It has been shown that the IHM increases the crosslink density of the network and the interaction potential between dipoles to help maintain the orientational order, and the generated NLO materials enhance the Tg, nonresonant second-order nonlinearity and temporal stability.

Crystal structure of bis(<i>N</i>,<i>N</i>,<i>N</i>-trimethylethanaminium) poly[bis(μ₂-heptaselenido-κ²Se¹,Se⁷)palladate(II)], C₁₀H₂₈N₂PdSe₁₄

Wang, Di,Yun, Hoseop,Kim, Kang-Woo unknown 2017 ZEITSCHRIFT FUR KRISTALLOGRAPHIE NEW CRYSTAL STRUC Vol.232 No.6

<P>C10H28N2PdSe14, monoclinic, P2(1)/c (no. 14), a=9.4146(7) angstrom, b=14.3446(11) angstrom, c=12.4731(9) angstrom, beta=113.403(2)degrees, V=1545.9(2) angstrom(3), Z=2, R-gt(F)=0.0510, wR(ref)(F-2)=0.1446, T=293 K.</P>

Poly(vinyl alcohol) Fibers with Excellent Mechanical Properties Produced by Reinforcement of Single-walled Graphene Oxide Nanoribbons with Complete Morphology Obtained by Freezedrying

Di Hu,Chao Xiao,Xia Wang,Xike Xiong,Jun Sun,Qiqi Zhuo,Jianjun Wang,Chuanxiang Qin,Lixing Dai 한국섬유공학회 2019 Fibers and polymers Vol.20 No.12

Nanofiller reinforcement is an effective approach to realize high performance of regular synthetic fibers. In thispaper, graphene oxide nanoribbons (GONRs) with complete morphology were prepared via unzipping single-walled carbonnanotubes (SWCNTs) through long-time freeze-drying after oxidation. GONRs derived from SWCNTs (SGONRs) did notneed any modification and could be directly added to poly(vinyl alcohol) (PVA) to form uniform dispersions and thencontinuous fibers were fabricated using wet spinning and hot-drawing. SGONRs provided abundant hydrogen bondinginteraction with PVA chains, so SGONRs could not only obviously improve the dispersibility in PVA, but also enhance themechanical properties of the composites. The tensile strength of PVA/SGONRs composite fibers with 0.4 wt% loading ofSGONRs reach 1032 MPa, improved by 121 % compared with PVA/SWCNTs fiber, and by 200 % with PVA fiber,respectively.

Design of the Spatial Sampling Scheme for Estimating the Cultivation Area of Garlic and Onion Using Satellite-Based and Unmanned Aerial Vehicle Remotely Sensed Data

Di Wang,Kyung-do Lee,Zhong-xin Chen,Sangil Na,Chan-won Park,Kyu-ho So 한국토양비료학회 2018 한국토양비료학회지 Vol.51 No.3

Garlic and onion are the most important vegetable crops in Korea. The accurate estimation of cultivation area for garlic and onion acreage is critical to predict the production of vegetable crops, adjust agricultural planting plan and ensure an effective supply of farm products. However, the plots cultivated with garlic and onion are very fragmentary and dispersive because the cross cropping commonly occurs in the two crops. Therefore, it is very difficult to establish the accurate identification of garlic and onion using satellite-based remotely sensed images alone. In case of tracking Hapcheon Gun, Korea as the sampling site, objective of this study was to formulate a spatial sampling scheme through combining satellite-based, unmanned aerial vehicle (UAV) remotely sensed images and the stratified sampling method for improving the estimated accuracy to cultivation area of garlic and onion. The results are shown that there was almost no classification error, when UAV remotely sensed image was used to retrieve the cultivation area of garlic and onion. The error found in the two crops classification using Rapid Eye satellite-based images, and the classification error for garlic was the larger than that of onion;. The variance for cultivation area of garlic and onion within each stratum can be significantly decreased, when the proportion of the cultivation area for two crops is accounted for one sampling unit. It was observed that the required sample size for meeting the designed extrapolation accuracy decreased with the stratification number of the sampled population. Comprehensively considering population extrapolating accuracy, sampling survey cost and rationality, 10 strata was the optimum stratification number. It was appeared that the spatial stratified sampling scheme combining satellite-based and UAV remotely sensed images had a high accuracy and stability for estimation of cultivation areas for the two crops, because both the relative error and CV of population extrapolation using this scheme was less than 10%.