http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
( Seo Young Kim ),( Su Sun An ),( Eun Young Lee ),( Seong Joon Moon ),( Ih Seop Chang ) 한국동물실험대체법학회 2007 한국동물실험대체법학회 학술대회집 Vol.2007 No.1
Evaluation of skin sensitization potential is a major part of safety assessment of new ingredients in cosmetics and drugs to be applied topically. In recent years, it has been postulated that dendritic cells respond to a variety of chemical allergens by up-regulating expression of various co-stimulatory molecules. It might provide the basis for an in vitro alternative approach for the identification of contact sensitizers. Several studies have shown that contact sensitizers induce co-stimulatory molecules on human hematopoietic cell lines. This study attempts to investigate the utility of THP-1 cell line and their co-stimulatory molecule expressions as a predictive in vitro assay system for contact sensitization by chemicals. We investigated the expression of CD54 and CD86 on the THP-1 cells using flow cytometry after a 24 h exposure to known sensitizers such as 1-chloro-2,4-dinitrobenzene (DNCB), hexylcinnamic aldehyde (HCA), eugenol (Eu), cobalt sulfate (Co) and known non-sensitizers such as sodium lauryl sulfate (SLS), benzalkonium chloride (BKC), dimehtyl sulfoxide (DMSO). The cytotoxicity of test materials was assessed with WST-1 assay to determine the appropriate dose and IC50 (50% inhibitory concentration) was obtained. Based on these data, three concentrations (0.1X, 0.5X, 1X of IC50) were selected. Relative fluorescence intensity (RFI) was used as an expression indicator. All allergens augmented CD54 and/or CD86 expression, while no augmentation was observed with non-allergens except SLS. From the expression of CD54 and/or CD86, we could classify the sensitizer/ non-sensitizer. These data suggest that expression of CD54 and/or CD86 in THP-1 cells may be able to classify the potency of allergen and this model can be a useful candidate for alternative test for skin sensitization.
Seo, Young-Kwon,Lee, Doo-Hoon,Shin, Youn-Ho,You, Bo-Young,Lee, Kyung-Mi,Song, Key-Yong,Seo, Seong-Jun,Whang, Sung-Joo,Kim, Young-Jin,Park, Chang-Seo,Chang, Ij-Seop,Park, Jung-Keug The Korean Society for Biotechnology and Bioengine 2003 Biotechnology and Bioprocess Engineering Vol.8 No.2
Obtaining a sufficient amount of healthy keratinocytes from a small tissue is difficult. However, ORS cells can be a good source of epithelium since they are easily obtainable and patients do not have to suffer from scar formation at donor sites. Accordingly, the current study modified the conventional primary culture technique to overcome the low propagation and easy aging of epithelial cells during culturing. In a conventional primary culture, the average yield of human ORS tells is 2.↑ $\times$ 10$^3$cells/follicle based on direct incubation in a trypsin (0.1%)/EDTA(0.02%) solution for 15 min at 37$^{\circ}C$, however, our modified method was able to obtain about 6.9 $\times$ 10$^3$cel1s/follicle using a two-step enzyme digestion method involving dispase (1.2 U/mL) and a trypsin (0.1%)/EDTA (0.02%) solution. Thus, the yield of primary cultured ORS cells could be increasd three times higher. Furthermore, a total of 2.0 $\times$ 10$^{7}$ cells was obtained in a serum-free medium. while a modified E-medium with mitomycin C-treated feeder tells produced a total of 6.3 $\times$ 10$^{7}$ Cel1s over 17 days When Starting With 7.5 $\times$ 10$^4$cells. Finally, We Confirmed the effectiveness of our ORS tell isolation method by presenting their ability for reconstructing the bioartificial skin epithelium in vitro
Joint Leveling Operation을 이용한 Kienbo¨ck씨 질환의 치료
이창섭,최장석,김영창,서승석,안기찬,전진욱,신재훈,나채호 인제대학교 2001 仁濟醫學 Vol.22 No.1
Purpose: We studied the Joint leveling operation in the treatment of Kienbo¨ck's disease. Materials and Methods: Ten patients from 1992 to 1997 who were followed up for more then 12 months were reviewed in terms of radiologic analysis and clinical results. Results: There were increase in Grip power and wrist ROM and also decrease of carpal collapse in 2 cases and no change in 8 cases. Conclusions: Joint leveling operation was good method for initial treatment of Kienbo¨ck disease compared to other procedures in terms of its simplicity and no change in carpal bone.
Hyung Yoon Kim,Sung-A Chang,Kye Hun Kim,Jae-Young Kim,Woo-Keun Seo,Hyung Seop Kim,Jeong-Sook Seo,Sung Hee Shin,Sang Jae Rhee,Sun Hwa Lee,Jung Sun Cho 한국심초음파학회 2021 Journal of Cardiovascular Imaging (J Cardiovasc Im Vol.29 No.3
BACKGROUND: The annual incidence of venous thromboembolism (VTE) is increasing, and the treatment pattern of oral anticoagulants (OACs) has changed with introduction of new oral anticoagulants (NOACs). The aims of this study were to assess the annual incidence of VTE in a Korean population and the change of treatment pattern with availability of NOACs using a population-based database. METHODS: Using the Korean National Health Insurance Services database, we identified patients diagnosed with VTE between 2009 and 2016. The annual prevalence of VTE and clinical characteristics and treatment pattern were investigated. The annual incidence of VTE was calculated using direct and indirect methods using the estimated Korean population in 2009 as the reference. RESULTS: The annual incidence of VTE in Korean has increased yearly from 23.9 per 100,000 in 2009 to 42.2 in 2016. The overall rate of OAC prescription for VTE treatment increased from 55.9% to 68% in the same time period. The rate of initiation of NOAC treatment greatly increased, particularly from 2013 onwards, with a 20-fold increase from 2009 to 2016 (2.1% vs. 54.3%). CONCLUSIONS: The annual incidence of VTE in Korea increased by almost two-fold from 2009 to 2016. The rate of initiation of NOAC treatment has increased substantially since 2013, and these agents have surpassed VKAs as the anticoagulant of choice for VTE. This temporal pattern of OAC prescription is consistent with the current clinical guidelines, which indicate NOACs over the warfarin in patients with VTE.
비구컵 재치환술에서 파쇄동종골 이식을 이용한 비구 결손의 재건술
안기찬(Ki Chan Ahn),최장석(Jang Seok Choi),김영창(Young Chang Kim),서승석(Seong Seok Seo),손영진(Young Jin Sohn),이창섭(Chang Seop Lee),전진욱(Jin Wook Jeon),손명환(Myung Hwan Sohn) 대한고관절학회 1999 Hip and Pelvis Vol.11 No.1
Twenty-one revisions of acetabular component from twenty patients were peformed using morselized allograft with a porous coated, screw-fixed hemisherical cup, between 1993 and 1996 at Inje university, Pusan Paik Hospital and were followed up for more than two years. Acetabular bone deficiency was classified by the AAOS classification. In our cases, 13 cases (60%) were cavitary deficiency and 8 cases (40%) were of cavitary deficiency combined with focal segmental deficiency. We evaluated our results by Harris hip score clinically and by the degree of cup migration, angular change of cup and resorption of graft radiologically. The definition of radiological loosening of cup was a 4mm migration of cup vertically or horizontally or an angular change of the cup of more than five degrees. The mean Harris hip score was improved 48 points to 85 points. The mean vertical migration was 1.4 mm and medial migration was 1.2 mm and no significant angular change of cup. There was no loosening of cup and rerevision. In conclusion, we had good results of acetabular cup with morselized allograft over a two year follow up period . Further long-term follow-up study is requred.
만성 활동성 간염에 병발한 Guillain-Barr 증후군과 6번 뇌신경 마비 1예
이창섭,이승옥,안득수,김대곤,이수택,서만욱,이흥범 대한소화기학회 2000 대한소화기학회지 Vol.35 No.2
Viral hepatitis may injure the central and peripheral nervous system, resulting in encephalitis, meningitis, myelitis, isolated pyramidal or extrapyramidal tract dysfunction, seizures, mononeuritis, plexitis, and psychiatric disturbances. Hepatitis associated Guillain-Barr syndrome mostly occurs as a complication of acute hepatitis, but is caused rarely by chronic active hepatitis B. Usually, Guillain-Barr syndrome is associated with peripheral nervous dysfunction and rarely involve central nervous system. However, Guillain-Barr syndrome with the 6th cranial nerve palsy resulted from chronic active hepatitis B is extremly rare. Recently, we experienced the case of Guillain-Barr syndrome with the 6th cranial nerve palsy in 21-year-old man with chronic active hepatitis B. He was improved with interferon-α2b and ordinary conservative treatment. Here, we report a rare clinical case of Guillain- Barr syndrome with the 6th cranial nerve palsy complicated by chronic active hepatitis B with a review of literatues.
Seo, Hyeuk,Lee, Yu Lim,Jung, Ki Chang,Park, Hyung Jo,Ha, Jun Seok,Kwak, Joon Seop,Jeong, Tak American Scientific Publishers 2018 Science Of Advanced Materials Vol.10 No.2
<P>This study compared the optical and electrical characteristics of GaN-based vertical light-emitting diodes (VLEDs) formed using various current blocking (CB) methods. The buried-type SiO2 CB method was found to be most effective in increasing the light output power of the VLEDs. In addition, the use of SiO2 CB layers (CBLs) produced less than 3% of the initial optical power drop and stable electrical properties after a 720 h reliability test. The O-2 plasma treatment, however, caused severe degradation of the VLED reliability due mainly to plasma-induced damage to the p-GaN layer during CBL formation.</P>
Chang, Boksoon,Han, Seo Goo,Kim, Wooyoul,Ko, Yousang,Song, Junwhi,Hong, Goohyeon,Eom, Jung Seop,Lee, Ji Hyun,Jhun, Byung Woo,Koh, Won-Jung The Korean Academy of Tuberculosis and Respiratory 2013 Tuberculosis and Respiratory Diseases Vol.75 No.1
Carbohydrate antigen 19-9 (CA 19-9) is a widely-used tumor marker in patients with pancreatic cancer. However, some patients with respiratory disease also exhibit elevated serum CA 19-9 levels. We report a case of normalization of elevated serum CA 19-9 levels after treatment of the nodular bronchiectatic form of Mycobacterium abscessus lung disease. A 40-year-old man visited our hospital because of chronic cough and sputum. A computed tomography scan revealed severe bronchiectasis in the right upper and right middle lobes. Nontuberculous mycobacteria were repeatedly isolated and identified as M. abscessus. The serum CA 19-9 level was elevated to 142.35 U/mL (normal range, <37 U/mL). Surgical resection was performed because of failure of sputum conversion after antibiotic treatment. The serum CA 19-9 level returned to the normal range after surgery. This case suggested that serum CA 19-9 levels could be elevated in patients with the nodular bronchiectatic form of M. abscessus lung disease.