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Allah Jurio Khaskheli,Muharam Ali,Syad Zakir Hussain Shah,Zohra Fatima Memon,Saleem Awan,Muhammad Ibrahim Khaskheli,Mohsin Ali Khaskheli,Bilqees Magsi,Zareen Qambrani,Asad Ali Khaskheli 한국식물생명공학회 2021 JOURNAL OF PLANT BIOTECHNOLOGY Vol.48 No.2
The present study aimed to evaluate the initiation, proliferation potential, and mass clonal production ability of a micropropagation system for banana through tissue culture. A total of 60 explants were cultured on basal media supplemented with various concentrations of BAP and NAA. Banana plants regenerated on MS basal medium (control) without the addition of BAP + NAA showed a significantly (P < 0.05) lower survival rate with no signs of shoots up to the end of the experimental period. The results further revealed that the performance in MSS-XI medium was almost 89%, followed by MSS-IX and MSS-X media, both of which showed performance up to 88%. In contrast, the performance in the MSS-XVI medium was less than 60%, at the less duration of time and highly shoot induction detected at MSS-XIII medium. The maximum number of shoots (4.9) was observed in the medium supplemented with growth adjuster MSS-XI, followed by the MSS-XII medium (4.5). Surprisingly, the best performance was observed for the MSR-VII medium approximately 16 days after initiation, while the lowest performance was observed with MSR-XI (approximately 31 days). The maximum rooting percentage (98%) was observed in the MSR-V to MSR-VIII media (98%), while the minimum rooting percentage was observed in MSR-XI (approximately 45%)
Farnesol production from <i>Escherichia coli</i> by harnessing the exogenous mevalonate pathway
Wang, Chonglong,Yoon, Sang‐,Hwal,Shah, Asad Ali,Chung, Young‐,Ryun,Kim, Jae‐,Yean,Choi, Eui‐,Sung,Keasling, Jay D.,Kim, Seon‐,Won Wiley Subscription Services, Inc., A Wiley Company 2010 Biotechnology and bioengineering Vol.107 No.3
<P><B>Abstract</B></P><P>Farnesol (FOH) production has been carried out in metabolically engineered <I>Escherichia coli</I>. FOH is formed through the depyrophosphorylation of farnesyl pyrophosphate (FPP), which is synthesized from isopentenyl pyrophosphate (IPP) and dimethylallyl pyrophosphate (DMAPP) by FPP synthase. In order to increase FPP synthesis, <I>E. coli</I> was metabolically engineered to overexpress <I>ispA</I> and to utilize the foreign mevalonate (MVA) pathway for the efficient synthesis of IPP and DMAPP. Two‐phase culture using a decane overlay of the culture broth was applied to reduce volatile loss of FOH produced during culture and to extract FOH from the culture broth. A FOH production of 135.5 mg/L was obtained from the recombinant <I>E. coli</I> harboring the pTispA and pSNA plasmids for <I>ispA</I> overexpression and MVA pathway utilization, respectively. It is interesting to observe that a large amount of FOH could be produced from <I>E. coli</I> without FOH synthase by the augmentation of FPP synthesis. Introduction of the exogenous MVA pathway enabled the dramatic production of FOH by <I>E. coli</I> while no detectable FOH production was observed in the endogenous MEP pathway‐only control. Biotechnol. Bioeng. 2010;107: 421–429. © 2010 Wiley Periodicals, Inc.</P>