대학생의 성교육 효과측정을 위한 기초 연구

장순복,최연순,강희선,박소미,Chang, Soon-Bok,Choi, Yun-Soon,Kang, Hee-Sun,Park, So-Mi 대한간호협회 1997 대한간호 Vol.36 No.2

This study was performed to provide preliminary data for the development of a useful instrument to measure the effect of sex education. The study was conducted with the voluntary participation of 155 college students enrolled in the course "Sexuality and Relationship" at Y university in Seoul. At the end of that course, they were asked to write freely about the change they had experienced on sexuality. All meaningful statements were elicited and classified into 7 categories; "Change of Knowledge", "Enlightenment", "Change of Attitude on Sexuality", "Buildup of Ability", "Buildup of. Identity", "Change of Emotion", "Change of Behavior:' There were significant changes in the areas of knowledge(28. 6%), enlightenment(27.4%), and attitudes(20.3%) about sexuality among these 7 categories. The Change of Knowledge category consists of 3 areas: "concretion of knowledge", "increase of information", and "correction of misunderstanding." In the category of Enlightenment, total 12 areas are included: "sex role", "erception of lack of knowledge", "importance of family", "life plan", "parent role", "value of life", "equality", "sexual autonomy", "importance of sexuality", "freedom of sexuality", "perception of sexual problem", and "meaning of love." The Attitude Change category consists of 8 areas. These are "being natural", "being progressive", "being sensitive", "being truthful", "being expressive", "being cautious", "being responsible", and "being confident". The category of Buildup of Ability includes 4 areas: "problem solving", "sex education", "relationship", and "communication". The category of Buildup of Identity includes "sexual identity", and "value of sexuality". The Emotional Change category includes 3 areas: "positive feeling", "negative feeling", and "breaking from negative feeling on sexuality". The Behavior Change category includes "sex-related behavioral change". In conclusion, up to now most researches on sex education effect measures only changes of knowledge, attitude, and behavior. But we believe the changes in "Enlightenment", "Buildup of Ability", "Buildup of Identity", "Change of Emotion", should be included in addition to knowledge, attitude, and behavior in the development of an instrument to measure the sex education effects. And the effect of sex education should measure the degree of learning of autonomy, enlightenment, and ability of behavior and so on rather than studying the simple changes in sexuality.enment", "Buildup of Ability", "Buildup of Identity", "Change of Emotion", should be included in addition to knowledge, attitude, and behavior in the development of an instrument to measure the sex education effects. And the effect of sex education should measure the degree of learning of autonomy, enlightenment, and ability of behavior and so on rather than studying the simple changes in sexuality.ng the simple changes in sexuality.

Age-Associated Changes in the Vascular Renin-Angiotensin System in Mice

Yoon, Hye Eun,Kim, Eun Nim,Kim, Min Young,Lim, Ji Hee,Jang, In-Ae,Ban, Tae Hyun,Shin, Seok Joon,Park, Cheol Whee,Chang, Yoon Sik,Choi, Bum Soon Hindawi Publishing Corporation 2016 Oxidative medicine and cellular longevity Vol.2016 No.-

<P><I>Background</I>. This study evaluated whether the change in the renin-angiotensin system (RAS) is associated with arterial aging in mice.<I> Methods</I>. Histologic changes and expressions of transforming growth factor-<I>β</I> (TGF-<I>β</I>), collagen IV,<I> fibronectin</I>, angiotensin II (Ang II), angiotensin-converting enzyme (ACE), angiotensin-converting enzyme 2 (ACE2), angiotensin II type 1 receptor (AT1R), angiotensin II type 2 receptor (AT2R), prorenin receptor (PRR), Mas receptor (MasR), endothelial nitric oxide synthase (eNOS), NADPH oxidase 2 and oxidase 4 (Nox2 and Nox4), 8-hydroxy-2′-deoxyguanosine (8-OHdG),<I> 3-nitrotyrosine</I>, and superoxide dismutase 1 and dismutase 2 (SOD1 and SOD2) were measured in the thoracic aortas from 2-month-old, 12-month-old, and 24-month-old C57/BL6 mice.<I> Results</I>. Twenty-four-month-old mice showed significantly increased aortic media thickness and expressions of TGF-<I>β</I>, collagen IV, and fibronectin, compared to 2-month-old and 12-month-old mice. The expressions of PRR, ACE, and Ang II, and AT1R-positive area significantly increased, whereas expressions of ACE2 and MasR and AT2R-positive area decreased with age. The expressions of phosphorylated serine<SUP>1177</SUP>-eNOS, SOD1, and SOD2 decreased, and the 8-OHdG-positive area and the 3-nitrotyrosine-positive area increased with age. The expression of Nox2 significantly increased with age, but that of Nox4 did not change.<I> Conclusions</I>. The enhanced PRR-ACE-Ang II-AT1R axis and reduced ACE2-MasR axis were associated with arterial aging in mice. </P>

배지의 무기염류와 sucrose 농도가 감자의 기내 shoot 증식과 기내경 삽목묘의 생존율에 미치는 영향

김희곤,임순희,안장순,송창길,박미경,강봉균 全南大學校 農業科學技術硏究所 1999 農業科學技術硏究 Vol.34 No.-

감자의 virus-free 기내경 삽목묘의 효율적인 대량 생산 체계의 확립을 위하여 생장점 배양으로 얻어진 감자의 기내경의 증식과 기내경의 순화삽목을 실시한 결과는 다음과 같다. 초대배양에서는 염류농도와 sucrose 농도가 높을수록 양호하였다. 즉, MS나 2MS에 sucrose를 6% 첨가한 배지에서 튼튼한 기내경이 생산되었다. 계대배양에서는 sucrose 농도가 높을수록 줄기의 길이는 단축되고 굵기는 커졌으며 생체중은 염류농도와 관계없이 3%의 sucrose가 첨가된 배지에서 무거웠다. 이들 유식물체의 경절을 삽목한 결과 계대배양 배지의 sucrose농도가 6%인 배지에서 생존율이 높은 경향이었다. In order to establish a mass propagation system of potato planting material, in vitro multiplication of virus-free shoot originated from meristem culture and sucrose concentrations on survival of cutting of the in vitro stem tried with various media containing different levels of MS salts(0.5x, 1x and 2x) and sucrose(1.0, 3.0, 6.0%), and the effects of MS salts elements were evaluated. Higher level of sucrose in the initial culture media favored the shoot growth, that is, both of 1MS and 2MS media containing 6.0% sugar produced stouter shoots. Higher level of sucrose in the subculture media also favored the development of compact stout shoots, but biomass production in terms of fresh weight was high on the media containing 3.0%sucrose, regardless of MS salts concentration. The survival rate of cuttings made of the in vitro stem segments tended to be higher in those obtained from subculture media containg 6.0%sucrose.

알코올에 의한 α-Tocopherol이 혈액성분에 미치는 영향

김영순,이상한,김창세,조만희 순천향의학연구소 1995 Journal of Soonchunhyang Medical Science Vol.1 No.2

This study was done in order to observe the effects of ethanol and a-tocopherol on the serum ALP, BUN and uric acid concentrations. The white Sprague-Dawley rats were classified with 4-grops : 0.9% NaCl administration was group A control, 25% ethanol administration for the each 5, 10 and 15 days was group B(Bl, B2 and B3), 25% ethanol administration for the each 5, 10 and 15 days and 50% a-tocopherol for the 2 and 7 days administration was group C (Cl and C2) and 50% a-tocopherol pretreated for the each 5, 10 and 16 days and 25% ethanol for the 2, 7 and the 10 days administration was group D(Dl, D2 and D3) The following results were obtained. 1. The changes of serum ALP activity was significantly increased in ethanol treated group. The group C and D was decreased. 2. The changes of serum BUN concentration between each group were not significant and however B2, B3, C2 and Dl was significantly increased. 3. The changes of serum uric acid concentration between each group were not significant. However group Cl and Dl was slightly decreased patterns.

중국여대생의 체형연구 Ⅰ

孫喜順,林珣,金孝淑,孫希定,장희경,鄭嶸 服飾文化學會 1999 服飾文化硏究 Vol.7 No.3

This research analyzes characterization and classification of body types for the Chinese women with body measurement values. The measurement has gauged in 1999 in Beijing Institute of Clothing Technology in China. 100 women of the Chinese college women aged 17 and 24 in China were mwasured for this research. In the results of frequency analysis, the tall of Chinese women were 158.37㎝ and also has less than 150㎝ high, and has thick under bust circumference, and has a group of more than 18㎝ for width of nipple to nipple. In the results of factor analysis. Factor 1 represented the degree of abesity, while factor 2 indicated the stature and the arm length. The body types are classified into five types by cluster analysis. The stature and weight varied according to types, leading to a classification focusing on the body size determined by stature and weight factors.

충주시 고등학생의 최근 4년간(1988-2001) B형간염 표면항원 및 항체 양성률 조사

김형수,이건세,장성훈,박수경,김청식,이창희,권혁중,정순섭 한국역학회 2002 Epidemiology and Health Vol.24 No.2

Objective : Since Korea has been known to be of the endemic area for hepatitis B virus(HBV) infection, vaccination for HBV has been recommended for all neonates in Korea. After starting vaccination in 1983, the change of sero-positivity of HBsAg and HBsAb is expected. This study was carried out to investigate the seropositive rate of hepatitis B virus surface antigen and antibody in high school freshmen students in Chungju-city. Methods and Materials : From 1998 to 2001, a total of 11,735 who were born in 1982 to 1986, were enrolled in this study. HBsAg and anti-HBs were tested by reversed passive hemagglutination and passive hemagglutination, respectively. Results : The study population was 11,735 students who were composed of 5,813 males (49.6%) and 5,914 females (50.4%). The HBsAg sero-positivity of male and female students was 3.25% (n=381) and 2.59% (n=153) respectively. The anti-HBs sero-positivity of male and female students was 62.2% (n=3,154) and 64.1% (n=2,969) respectively. There are significant difference observed in comparison of HBsAg and anti-HBs seropositivity according to sex (p<0.05). From 1982 to 1986 on birth year, anti-HBs sero-positivity has been increased significantly from 63.3% to 73.3%. But the change of HBsAg sero-positivity has not been changed significantly. Conclusions : As compared with previous studies of HBsAg and anti-HBs, the sero-positive rate of HBsAg has not been changed but that of HBsAb has been increased significantly. This result revealed that the effect of HBV vaccination program was successful.

제왕절개술을 위한 경막외 마취시 술전 투여 수액이 신생아 혈당치에 미치는 영향

이혜원,김선희,신정순,장성호,임혜자,채병국 대한마취과학회 1993 Korean Journal of Anesthesiology Vol.26 No.4

It is common for patient undergoing cesarean section under epidural anesthesia to have lower blood pressure because of not only supine hypotension syndrome but vasodilation due to sympathetic blockade. So it is necessary to give large volume of fluid before perfarming epidural anesthesia to prevent hypotension. When we use the dextrose containing fluid for that purpose, newborn baby could have a high blood glucose at delivery and low blood glucose level due to high insulin level after birth. The authors examined the blood glucose level at delivery and two hours after birth following use of 5% dextrose in lactated Ringers solution(HD group, n=24) or lactated Ringers solution (HS group, n=24) for prevention of hypotension during epidural anesthesia for cesarean section. The results were as follows; 1) The total amount of fluid was 789.6±264.2 ml in HD group and 741.1±253.5 ml in HS group. 2) The glucose level of mother at delivery was 300.9±76.3 mg/dl in HD group and 98.7±24.6 mg/dl in HS group and there wastistical significance(p$lt;0.01). 3) In HD group, the glucose level of newborn baby was 212.2±57.2 mg/dl at delivery and 465.±20.3 mg/dl at 2 hours after birth. The change of amount during 2 hours after birth was highly related to the glucose level at birth. Higher level of glucose at birth, larger amount of change during 2 hours after birth. 4) In HS group, the glucose level of newborn baby was 66.8±10.2 mg/dl at birth and 67.0±12.1 mg/dl at 2 hours after birth. There was no significant change.

폐동맥고혈압이 동반된 만성폐쇄성폐질환 환자에서 혈중 Brain Natriuretic Peptide, 폐동맥압 및 St. George Respiratory Questionnaire의 상관성과 안지 오텐신전환효소억제제 치료 효과

김명아 ( Myung A Kim ),김덕겸 ( Deog Kyeom Kim ),이창훈 ( Chang Hoon Lee ),정희순 ( Hee Soon Chung ) 대한결핵 및 호흡기학회 2010 Tuberculosis and Respiratory Diseases Vol.68 No.5

Background: Pulmonary hypertension is considered as a poor prognosis factor in patients with chronic obstructive pulmonary disease (COPD). There has been reported brain natriuretic peptide (pro-BNP) is related with increased right ventricular (RV) workloads. However, there are few studies that evaluate the relationship between BNP and pulmonary arterial pressure (PAP), RV function and St. George Respiratory Questionnaire (SGRQ) score in patients with COPD, and the effects of angiotensin converting enzyme inhibitor (ACEI) on these parameters. Methods: Pulmonary function test, echocardiography, blood BNP, and SGRQ score were evaluated in stabilized moderate degree COPD patients (FEV1/FVC<70%, 50%≤FEV1<80%) aged 45 years and over, without worsening of symptoms within recent 3 months. After treating with ramipril 10 mg for 3 months, the same evaluation was repeated. Results: Twenty-two patients were included in this study. BNP was significantly correlated with PAP (Pearson coefficient ρ=0.51, p=0.02), but not with RV ejection fraction (EF) and predicted FEV1%. The values for predicted FEV1% showed significant correlation with SGRQ total score and activity score, but not with BNP or PAP. After ramipril treatment, PAP showed significant decrease (42.8±8.1vs.34.5±4.5 mm Hg p=0.0003), tricuspid annular plane systolic excursion significant increase (21.5±3.3vs.22.7±3.1 mm p=0.009). BNP showed a tendency to decrease without statistical significance (40.8±59.6vs.18.0±9.1 pg/mL p=0.55). SGRQ scores showed no significant change. Conclusion: BNP showed significant correlation with resting PAP, which means BNP could be used as markers for pulmonary hypertension. Treatment with ACEI didn`t show significant change in the Level of BNP, while pulmonary hypertension and RV function were improved.

소나무 재생버섯(Fomitopsis pinicola Jeseng) 다당류의 추출 특성

장경호,신진기,이명예,이상일,김정숙,오승희,김순동 동아시아식생활학회 2005 동아시아식생활학회지 Vol.15 No.1

This study was conducted to investigate the extraction characteristics of the polysaccharide from Jeseng mushroom (Fomitopsis pinicola Jeseng). Yields of the polysaccharide extracted from powdered mushroom by autoclaving(120, 30 min) with water at different pH and salt concentration were 8.2~9.2% in pH 5~11, 4.7~5.5% in 1.5% salt solution, respectively. The yield by the 0.05~1.0 N KOH-extraction was ranged 3.45~13.20%, while that by HAS-extraction(homogenizing after KOH swelling) using 1~2.5 N KOH 73.6~78.4%. Content of carbohydrate, protein, lipid and ash of the crude polysaccharide extracted from fruits body and its cultured mycelium by method of water extraction, KOH extraction(0.005~1N) and HAS-extraction were ranged 86.5~92.6%, 2.3~13.1%, 0.1~4.2% and 0.1~1.7%, respectively. The polysaccharide were composed of 62.0~77.8 g/g of pentose, 138.0~187.8 g/g of hexose and 21.2~117.3 ㎎/g of protein. From these results, the polysaccharide extracted was supposed to be a protein-bound polysaccharide.

대장암 환자에서 Survivin mRNA의 발현과 간 전이와의 연관성

최종순,장희경 고신대학교의과대학 2008 고신대학교 의과대학 학술지 Vol.23 No.1

연구배경 및 목적 Survivin은 새로이 고사(아포프토시스)-억제 유전자군에 추가된 유전자이다. Survivin 발현이 대장암에서 보고는 되고 있으나, 상이한 결과들로 논란이 많으며, 임상적 의의에 대해서도 불명확하다. 더욱이 이 유전자는 암 치료나 예방적 항암치료의 표적으로의 가능성이 제기되고 있다. 대장암 survivin 유전자의 발현을 조사하고 임상 및 예후 인자들과의 관련성을 분석하여 대장암의 발생과 임상 측면에서이들 유전자의 역할을 이해하고 예방과 치료에 이용할 수 있는 지를 조사하고자 하였다. 대상 및 방법 다양한 병기의 37예의 신선한 대장암 수술 조직을 대상으로 하였다. 암 조직과 주위 비종양성 조직에서 전체 mRNA를 분리하여 역전사-연쇄중합효소반응으로 survivin mRNA를 검출하였다. PCR 산물을 확인하기 위하여 아클로닝후에 DNA 염기 분석을 시행하였다. 결 과 대장에서 Survivin의 발현율은 대장암 조직에서는 94.6%였고, 비종양성 조직에서는 51.4%으로, 대장암 조직에서 survivin의 발현이 높았으나, 비종양성 조직에서의 경계성 영역의 유의한 차이가 인정되었다(0.05<p=0.065< 0.1). 연령, 성별, 침윤 깊이, 림프절 전이, 혈관과 신경 침범, 간으로의 전이 등의 임상 및 병리학적 인자들과 관련성은 없었다. 결 론 survivin은 대장암에서 암특이적으로 발현되지 않았다 (0.05<p=0.065< 0.1). 대장암의 치료나 chemoprevention에서 SSX(synovial sarcoma on X chromosome gene)와 survivin의 이용은 가능하나 제한적일 것으로 생각된다. SSX와 survivin 발현의 상관성이 인정되지 않으므로, 대장암에서 SSX은 survivin-관련 항아포토시스 경로와는 무관할 것으로 생각된다. Background : Survivin is a novel member of the inhibitors of apoptosis proteins (IAP) gene family. Although survivin expression has been reported in colon cancer, the results are still controversial and its clinical significanceincluding chemoprevention remains unclear. Some reports described thegene as a potential target in anticancer or chemopreventive therapy. To facilitate understanding of survivin in the colon cancer, the clinicopathological significance was investigated with the expression of survivin. Methods : Surgical specimen were obtained from a total 37 cases of consecutive patients with various stages of colorectal carcinoma who had undergone a resection. To determine survivin mRNA in colorectal carcinomas and adjacent normal colorectal tissue samples, total RNA was isolated from each of the samples after lysis in quanidinium isothiocyanate and phenol extraction and reverse-transcription polymerase chain reaction was done. The PCR products were confirmed by DNA sequencing after subcloning. Results : The positive rate of survivin in cancer tissue was 94.6%(35/37) and that of non-neoplastic colorectal tissue was 51.4%(19/37). S urvivin expression t ended to i ncrease in c ancer tissue, but h as s tatistically m arginal significance(0.05<p=0.065< 0.1). The correlation of survivin expressions to age, gender, invasion depth, lymph node metastasis, vessel and perineural invasions, and liver metastasis was not found. Conclusions : The expression of survivin appears not to be eligible to differentiate malignant from benign lesions in colon, even though it has marginal significance(0.05<p=0.065< 0.1). The role of survivin-associated apoptosis may be unnoticeable, in the light of insignificant correlation of gene expression to the other prognostic factors in colon cancer.