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최연순,이희성,이근배 ( Yun Soon Choi,Hi Sung Lee,Keun Bai Lee ) 생화학분자생물학회 1973 BMB Reports Vol.6 No.3
Glyoxylate reductase (glycollate-NAD oxidoreductase, 1. 1. 1. 26) has been prepared from 20,000 x g supernatant fraction of homogenate of germinating soy bean seed cotyledons and purified by ammonium sulfate precipitation, Sephadex G-200 gel filtration and DEAE-cellulose chromatography. The molecular weight of the enzyme was estimated to be about 158,000 by Sephadex G-100 gel filtration. The purified enzyme has been dissociated into a molecule one fourth the molecular weight of the native enzyme by treatment with 1 per cent sodium dodecyl sulfate, β-mercaptoethanol and Tris-buffer at pH 7.1 for 30 min. The molecular weight of the subunit was estimated to be approximately 41,500±4,000 by polyacrylamide gel electrophoresis. Amino acid composition of the above enzyme molecule are also presented. Some molecular properties of the subunit are being investigated.
최연순,Hee Kyoung Shin,홍대용,김장락,강윤식,정백근,박기수,이기효 대한예방의학회 2019 Journal of Preventive Medicine and Public Health Vol.52 No.1
Objectives: The purpose of this study was to investigate the role of self-esteem as a moderator of the factors influencing suicidality among middle-schoolers. Methods: Moderated multiple regression analysis was applied to assess the influence of happiness, depression, and hostility on suicidality and to determine the degree to which self-esteem served as a moderator of those relationships. Data were collected from 268 students at a middle school in Busan, Korea, using a self-administered structured questionnaire. Results: Happiness, depression, and hostility had significant direct effects on suicidality. Self-esteem showed no direct effect, but had a significant moderating effect on the relationship between hostility and suicidal behavior. Conclusions: These results suggest that various interventions, such as counseling programs, should be designed to alleviate hostility and depression and to enhance happiness and self-esteem among early adolescents.