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Cha, Hoon-Suk,Bae, Eun-Kyung,Ahn, Joong-Kyong,Lee, Jae-Joon,Ahn, Kwang-Sung,Koh, Eun-Mi Korean Society for Biochemistry and Molecular Bion 2010 Experimental and molecular medicine Vol.42 No.6
Inadequate apoptosis contributes to synovial hyperplasia in rheumatoid arthritis (RA). Recent study shows that low expression of Puma might be partially responsible for the decreased apoptosis of fibroblast-like synoviocytes (FLS). Slug, a highly conserved zinc finger transcriptional repressor, is known to antagonize apoptosis of hematopoietic progenitor cells by repressing Puma transactivation. In this study, we examined the expression and function of Slug in RA FLS. Slug mRNA expression was measured in the synovial tissue (ST) and FLS obtained from RA and osteoarthritis patients. Slug and Puma mRNA expression in FLS by apoptotic stimuli were measured by real-time PCR analysis. FLS were transfected with control siRNA or Slug siRNA. Apoptosis was quantified by trypan blue exclusion, DNA fragmentation and caspase-3 assay. RA ST expressed higher level of Slug mRNA compared with osteoarthritis ST. Slug was significantly induced by hydrogen peroxide ($H_2O_2$) but not by exogenous p53 in RA FLS. Puma induction by $H_2O_2$ stimulation was significantly higher in Slug siRNA-transfected FLS compared with control siRNA-transfected FLS. After $H_2O_2$ stimulation, viable cell number was significantly lower in Slug siRNA-transfected FLS compared with control siRNA-transfected FLS. Apoptosis enhancing effect of Slug siRNA was further confirmed by ELISA that detects cytoplasmic histone-associated DNA fragments and caspase-3 assay. These data demonstrate that Slug is overexpressed in RA ST and that suppression of Slug gene facilitates apoptosis of FLS by increasing Puma transactivation. Slug may therefore represent a potential therapeutic target in RA.
Sang Chul Shim,Young Gil Kwon,Chil Hoon Doh,Byung Won Woo,Jin Ook Baeg,Hong Seok Kim,Tae Jeong Kim,Dong Ho Lee,Young Woo Kwak,Jin Soon Cha,Hyung Soo Lee,Jae Kook Uhm,Young Bae Park Korean Chemical Society 1990 Bulletin of the Korean Chemical Society Vol.11 No.2
Ethanolic tetra carbonylhydridoferrate solution combined with dialdehyde (no of carbon; 4,5,6) is very efficient for the selective transformation of amino group into N-heterocyclic compound. However, a large variety of both aliphatic and aromatic amines react with the ferrate-pimelaldehyde at room temperature under an atmospheric pressure of carbon monoxide to give the corresponding N-(cyclohexylmethyl)-N-alkyiamine derivatives in moderate yields instead of the corresponding N-substituted perhydroazocine derivatives.
( Sung Hee Jung ),( Yun Jung Lee ),( Sung Hoon Lee ),( Hyang Le Lee ),( Hyeon Woong Yang ),( An Na Kim ),( Sang Woo Cha ) 대한소화기학회 2007 SIDDS Vol.9 No.-
Background/Alms: The frequency of intestinal involvement in Behcet`s disease is known to be 3~25% with geographic differences which is higher in East Asian patients including Koreans. We tried to find out the reliable gastrointestinal symptoms and laboratory data which can predict the verification of intestinal Behcet`s disease by colonoscopy. Methods: Between April 2004 and August 2007, 29 patients underwent colonoscopy among 186 patients who were diagnosed with complete or incomplete type Behcet`s disease at the department of rheumatology internal medicine, Eulji medical school hospital, Daejeon, Korea. We analyzed the relations among their symptoms, colonoscopic findings, and laboratory data, retrospectively using medical records. A p value of less than 0.05 was considered as statistically significant. Results: The mean age of the 29 patients was 43.3 years; most (23/29) of them were female. Twenty two (75.9%) patients complained of gastrointestinal symptoms, such as abdominal pain (41.1%), hematochezia, diarrhea, loose stool. On laboratory examination, mean Hb was 12.3 g/dL, ESR 31.8 mm/hr, and CRP 2.30 mg/dL. On colonoscopy, lesions were found in only 50% of the symptomatic patients. There was no significant relation with the presence of gastrointestinal symptoms and colonoscopic lesions (p>0.05). Hematochezia (p=0.004) and anemia (p=0.042) were strongly correlated to colonoscopic lesions unlike diarrhea, loose stool, abdominal pain, ESR, and CRP elevation Conclusions: We should consider the colonoscopic examination including terminal ileum to the Behcet`s disease patients who showed hematochezia and anemia for the early recognition of intestinal Behcet`s disease.
Optimal Conditions for Chitinase Production by Serratia marcescens
Cha, Jin-Myeong,Cheong, Kyung-Hoon,Cha, Wol-Suk,Choi, Du-Bok,Roh, Sung-Hee,Kim, Sun-Il The Korean Society for Biotechnology and Bioengine 2004 Biotechnology and Bioprocess Engineering Vol.9 No.4
A chitinase-producing bacterium was isolated from seashore mud around Beobseongpo in Chunmam province through the use of a selective enrichment culture. The best chitinase producing strain was isolated and identified as Serratia marcescens KY from its characteristics. For effective production of chitinase, optimum pH, temperature, and agitation speed were investigated in flask cultures. The optimum pH using Serratia marcescens KY was between pH 6 and 7 and the chitinase produced was 37.9 unit/mL. On the other hand, the optimal pH of the Serratia marcescens ATCC 27117 was 7.5, and the produced amount of chitinase was 35.2 unit/mL. The optimal temperature for chitinase production for Serratia marcescens KY and Serratia marcescens ATCC 27117 was $30^{\circ}$. The cell growth pattern at different temperature was almost identical to the chitinase production. To investigate the optimal shaking speed under optimal culture, speeds were varied in the range of 0∼300 rpm. The maximum production of chitinase was carried at 200 rpm although the cell growth was the highest at 150 rpm. It indicates that oxygen adjustment is required for the maximum chitinase production. Using optimal conditions, batch cultures for comparing Serratia marcescens KY and Serratia marcescens ATCC 27117 were carried out in a 5 L fermentor. The oxygen consumption was increased with the increase of culture. Especially, at 120 h of culture Serratia marcescens KY and Serratia marcescens ATCC 27117 produced 38.3 unit/mL, and 33.5 unit/mL, respectively.
Cha, Hyojung,Chung, Dae Sung,Bae, Suk Young,Lee, Min‐,Jung,An, Tae Kyu,Hwang, Jihun,Kim, Kyung Hwan,Kim, Yun‐,Hi,Choi, Dong Hoon,Park, Chan Eon WILEY‐VCH Verlag 2013 Advanced functional materials Vol.23 No.12
<P><B>Abstract</B></P><P>Two anthracene‐based star‐shaped conjugated small molecules, 5′,5″‐(9,10‐bis((4‐hexylphenyl)ethynyl)anthracene‐2,6‐diyl)bis(5‐hexyl‐2,2′‐bithiophene), HBantHBT, and 5′,5″‐(9,10‐bis(phenylethynyl)anthracene‐2,6‐diyl)bis(5‐hexyl‐2,2′‐bithiophene), BantHBT, are used as electron‐cascade donor materials by incorporating them into organic photovoltaic cells prepared using a poly((5,5‐E‐alpha‐((2‐thienyl)methylene)‐2‐thiopheneacetonitrile)‐alt‐2,6‐[(1,5‐didecyloxy)naphthalene])) (PBTADN):[6,6]‐phenyl‐C71‐butyric acid methyl ester (PC<SUB>71</SUB>BM) blend. The small molecules penetrate the PBTADN:PC<SUB>71</SUB>BM blend layer to yield complementary absorption spectra through appropriate energy level alignment and optimal domain sizes for charge carrier transfer. A high short‐circuit current (<I>J</I><SUB>SC</SUB>) and fill factor (FF) are obtained using solar cells prepared with the ternary blend. The highest photovoltaic performance of the PBTADN:<B>BantHBT</B>:PC<SUB>71</SUB>BM blend solar cells is characterized by a <I>J</I><SUB>SC</SUB> of 11.0 mA cm<SUP>−2</SUP>, an open circuit voltage (<I>V</I><SUB>OC</SUB>) of 0.91 V, a FF of 56.4%, and a power conversion efficiency (PCE) of 5.6% under AM1.5G illumination (with a high intensity of 100 mW<SUP>−2</SUP>). The effects of the small molecules on the ternary blend are investigated by comparison with the traditional poly(3‐hexylthiophene) (P3HT):[6,6]‐phenyl‐C61‐butyric acid methyl ester (PC<SUB>61</SUB>BM) system.</P>
Sang Hoon Lee,Chun Geon Park,Jeong Hoon Lee,Yeon Bok Kim,Young Guk Kim,Seon Woo Cha,Gwan Hou Kim,Hyo Jin Kim,Chang Suk Huh,Sang Won Lee 한국약용작물학회 2016 한국약용작물학술대회 발표집 Vol.2016 No.05
Background : This study was development of moisture tolerance and high-yielding Rehmannia glutinosa cultivar. Methods and Results : Segang is developed by the medicinal crop breeding team of National Institute of Horticulture and Herbal Science(NIHHS), Rural Development Administration(RDA), during the period from 2005 to 2015. The reproduction of Rehmannia glutinosa has been accomplished mainly by vegetative propagation with its seedlings have many variants. The cultivar was selected from seedling of Jihwang 1(check variety). The plant type of Segang is some rising from ground. Regional yield trials conducted at three site from 2014 to 2015. The root yield of Segang was 21.1ton per hectare, which was increased 12% compared with Jihwang 1. Also, Segang has higher catalpol content and dried root ratio compared with Jihwang 1. Conclusion : Segang is a moisture tolerance and high-yielding Rehmannia glutinosa cultivar.
Cha, Moon‐,Yong,Kwon, Yoo‐,Wook,Ahn, Hyo‐,Suk,Jeong, Hyobin,Lee, Yong Yook,Moon, Minho,Baik, Sung Hoon,Kim, Dong Kyu,Song, Hyundong,Yi, Eugene C.,Hwang, Daehee,Kim, Hyo‐,Soo,Mo John Wiley and Sons Inc. 2017 Stem cells translational medicine Vol.6 No.1
<P><B>Abstract</B></P><P>Transplantation of stem cells into the brain attenuates functional deficits in the central nervous system via cell replacement, the release of specific neurotransmitters, and the production of neurotrophic factors. To identify patient‐specific and safe stem cells for treating Alzheimer's disease (AD), we generated induced pluripotent stem cells (iPSCs) derived from mouse skin fibroblasts by treating protein extracts of embryonic stem cells. These reprogrammed cells were pluripotent but nontumorigenic. Here, we report that protein‐iPSCs differentiated into glial cells and decreased plaque depositions in the 5XFAD transgenic AD mouse model. We also found that transplanted protein‐iPSCs mitigated the cognitive dysfunction observed in these mice. Proteomic analysis revealed that oligodendrocyte‐related genes were upregulated in brains injected with protein‐iPSCs, providing new insights into the potential function of protein‐iPSCs. Taken together, our data indicate that protein‐iPSCs might be a promising therapeutic approach for AD. S<SMALL>TEM</SMALL> C<SMALL>ELLS</SMALL> T<SMALL>RANSLATIONAL</SMALL> M<SMALL>EDICINE</SMALL><I>2017;6:293–305</I></P>
Sung Hoon Yoon,Kil Soo Lee,Jae Sang Cha,Vinayagam Mariappan,Min Woo Lee,Deok Gun Woo,Jeong Uk Kim 국제문화기술진흥원 2020 International Journal of Advanced Culture Technolo Vol.8 No.1
The human centric lighting (HCL) control is a major focus point of the smart lighting system design to provide energy efficient and people mood rhythmic motivation lighting in smart buildings. This paper proposes the HCL control using indoor surveillance camera to improve the human motivation and well-beings in the indoor environments like residential and industrial buildings. In this proposed approach, the indoor surveillance camera video streams are used to predict the day lights and occupancy, occupancy specific emotional features predictions using the advanced computer vision techniques, and this human centric features are transmitted to the smart building light management system. The smart building light management system connected with internet of things (IoT) featured lighting devices and controls the light illumination of the objective human specific lighting devices. The proposed concept experimental model implemented using RGB LED lighting devices connected with IoT features open-source controller in the network along with networked video surveillance solution. The experiment results are verified with custom made automatic lighting control demon application integrated with OpenCV framework based computer vision methods to predict the human centric features and based on the estimated features the lighting illumination level and colors are controlled automatically. The experiment results received from the demon system are analyzed and used for the real-time development of a lighting system control strategy.
( Sang Hyeok Im ),( Dong Hwan Kim ),( Young Hoon La ),( Nam Jin Kim ),( Cha Won Hwang ),( Jae Min Cha ),( Bong Ki Ryu ) 대한금속재료학회 ( 구 대한금속학회 ) 2010 재료마당 Vol.23 No.3
This study looks at crystallization and sintering behavior in B2O3 containing Li2O-Al2O3-SiO2 glass powder. The sintered sample was produced with glass powders measuring 44 μm. The relative density and transmittance of the sintered samples showed the highest value at the temperature of 650°C. At temperatures higher than 650°C, crystal growth occurred to decrease the densification of B2O3-Li2O-Al2O3-SiO2 (BLAS) glass powder. The main crystalline phase in the glass powder was a-spodumene. From non-isothermal differential thermal analysis, the crystallization of particles (Φ=44 μm) was observed at 640°C to 684°C with respect to the heating rate. The activation energy of crystallization (E(C)) and the Avrami constant (n) calculated by Kissinger and Ozawa equations indicated that the surface and the bulk crystallization occurred simultaneously in the glass. The optimum sintering temperature of this glass powder was 650°C without crystal growth of β-spodumene, although nucleation or nano-crystal growth occurred.