석탄폐석 및 광재의 유해물질 침출특성 연구

우영국,강성환,임재명,한동준,김병욱 강원대학교 석재복합신소재제품연구센터 1996 석재연 논문집 Vol.1 No.-

광산에서 배출되는 광재폐기물은 산과 계곡에 투기 또는 매립되어 강우등에 의하여 주변 소하천을 오염시키고 있다. 따라서 본 연구는 광재의 물리적특성과 이화학적 특성을 조사하고 회분식과 칼럼식실험을 수행하여 오염물질의 침출특성을 고찰하고자 하였다. 실험결과, 다음과 같은 결론을 얻을 수 있었다. 1) 광재는 점토질 및 실트질의 크기를 지니고 있으나 점착력과 소성적 성질이 없는 사질토의 특성을 지니고 있다. 2) 광재의 투수계수는 대부분 10(???)³-10(???)cm/sec의 범위이며, 일반 사질토의 투수계수보다 낮음을 알 수 있었다. 3) 회분식과 칼럼식 용출시험의 결과, 대상 광산의 광재폐기물 일부에서 기준치 이상의 중금속농도가 침출되었으며, 이에대한 방지대책의 심각성을 알 수 있었다. 4) 광재 폐기물의 칼럼식 용출시험 결과 kg폐기물 당 인공강우 투여량 비가 증가할수록 침출수의 중금속 및 유해물질 농도는 크게 감소되어 어느정도 진행된 이후부터는 대부분 일정한 농도가 유지되나, 산성강우시 침출정도 및 침출시간은 지속되는 것을 알 수 있었다. 5) 광재는 많은 중금속과 유해물질을 함유하고 있으나 양적인면에서 자원화의 가치가 높을 것으로 판단된다. 따라서 광재내의 자원회수, 건설 재료등의 재활용및 자원화 연구가 필요하다. Tailing wastes producedat metal mines have been disposed of by dumping or landfiling in depression. Mine drainage developed at pile of the tailing wastes has high concentrations of various kinds of heavy metals and harzardous materials, and these contaminants are leached out from the tailing wastes by water infiltration or when they collapse. In this study, both batch test and column test were performed in order to investigate the physical and leaching characteristics of tailing wastes. The batch test results were compared with those by EPT and DLT, and column test was carried out at acidic and neutral pH ranges. The results showed that high concentrations of heavy metals and sulfate were contained in the leachate of the some tailing wastes. In column test, levels of heavy metals in the leachate produced during the initial rainfall period were found higher.

1-Phenylindole Ester류의 합성과 반응성 연구

崔舜圭,金兪瑛,兪丙國,李龍均,鄭大一 東亞大學校附設基礎科學硏究所 1993 基礎科學硏究論文集 Vol.10 No.1

일반적인 dienophile에 대한 pyrrole의 반응은 pyrrole이 diene system으로 작용된 [4+2] 고리부가반응과 pyrrole의 α-탄소 위치에 부가되는 Michael형태의 반응이 알려져 있다.

미세알부민뇨를 동반한 인슐린 비의존성 당뇨병 환자에서 혈장 Endothelin의 변화

서예경,정상원,남직화,신병호,전종민,김정국,김동희,하승우,김보완 慶北大學校 醫科大學 1996 慶北醫大誌 Vol.37 No.3

목적 : 혈관내피세포에서 생성되는 endothelin(ET)는 강력한 혈관수축작용을 가진 펩타이드로 당뇨병 환자에서 혈관내피세포의 손상여부에 따라 혈중 농도가 증가할 수 있으며 혈관합병증과 관련이 있다는 보고가 있다. 그러나. 혈중 ET치의 상승과 당뇨병성 합병증의 상관성에 대해서는 논란이 많다. 이에 저자들은 인슐린 비의존성 당뇨병 환자에서 미세알부민뇨의 동반유무에 따른 혈중 ET치의 변화 및 혈장 ET치와 요중 알부민 배설률과의 상관성을 검토하였다. 대상 및 방법 : 인슐린 비의존성 당뇨병 환자중 미세알부민뇨를 보인 22명과 정상알부민뇨를 보인 43명을 대상으로 하였으며 혈장 ET를 측정하여 요중 알부민 배설률, 당뇨병성 망막증 및 당뇨병 관련인자들과의 관계를 비교하였다. 결과 : 당뇨병성 망막증이 합병된 군에서 망막증이 합병되지 않은 군에 비하여 혈장 ET치가 유의하게 높았던 반면 (각각 1.9±0.66, 1.5±0.76pg/㎖, p<0.05) 미세알부민뇨를 보인 군과 정상알부민뇨를 보인 군사이에 혈장 ET치는 통계학적으로 유의한 차이가 없었다(각각 1.9±1.14, 1.7±0.69pg/㎖). 또한 요중 알부민 배설률과 혈장 ET치 사이에 유의한 상관관계는 없었으며 미세알부민뇨를 동반한 인슐린 비의존성 당뇨병 환자군에서 혈장 ET치는 당뇨병의 관련인자들과도 유의한 상관관계가 없었다. 결론 : 인슐린 비의존 당뇨병 환자에서 혈장 ET치가 초기 당뇨병성 신증의 합병여부나 미세알부민뇨의 정도를 반영하기는 어려울 것임을 시사한다. Background : Endothelin(ET), a potent vasoconstritor peptide released from vascular endothelial cells, is known to be increased in diabetic patients with vascular damage and related with diabetic angiopathy. However, whether plasma ET levels may be related to diabetic complications is controversial. We studied the change of ET levels in non-insulin dependent diabetics with microalbuminuria and relation between ET and urinary albumin excretion rate. Methods : Plasma ET levels of 22 non-insulin dependent diabetics with microalbuminuria and 43 patients with normoalbuminuria were measured and relations of plasma ET to diabetic complications such as urinary albumin excretion rate, retinopathy and other associated factors were studied. Results : Plasma ET levels were significantly higher in patients with diabetic retinopathy than those without retinopathy(1.9 ± 0.66 vs 1.5 ± 0.76 pg/㎖. p<0.05). The difference, however, between diabetics with and without microalbuminuria was not significant(1.9 ± 1.14 vs 1.7 ± 0.69 pg/㎖). There were no correlations of plasma ET to urinary albumin excretion rate and to the other associated factors in non-insulin dependent diabetics. Conclusion : These results suggest that plasma ET levels may not seem to act as a predictor of diabetic nephropathy or microalbuminuria in non-insulin dependent diabetics.

만성폐쇄성 폐질환 환자에서 적혈구 항산화효소의 변화

국기용,이만재,조희충,임영국,박일,김원식,이승일,이병래 朝鮮大學校 附設 醫學硏究所 1993 The Medical Journal of Chosun University Vol.18 No.1

Toxic oxygen free radicals have been implicated as important pathologic mediators in many clinical disordesrs. Enhandng the intracellular content of antioxidant enzymes can provide means of limiting biological damage caused by oxygen free radicals. Many investigations have examined the potential of various oxyradical scavengers to modulate oxyradical injury. The oxygen free radicals play a role in the pathogenesis of chronic obstructive pulmonary disease. To investigate the pulmonary oxyradical injury and the protective role of antioxidant enzymes, author measured the amount of thiobarbituric acid reactants, the activiteies of antioxidant enzymes (superoxide dismutase, glutathione peroxidase, and catalase) and the sulfhydryl groups in serum and red blood cells from the chronic obstructive pulmonary disease groups and the normal controls. The thiobarbituric acid reactant in chronic obstructive pulmonary disease groups was increased than the controls in both serum and red blood cells, and the superoxide dismutase activity in red blood cells was no statistically difference in both groups. But, the glutathione peroxidase and catalase activities were lowered in chronic obstructive pulmonary disease groups than the controls. The sulfhydryl groups in serum and red blood cells were no statistically difference in both groups. These results suggest that the increased thiobarbituric acid reactant in chronic obstructive pulmonary disease groups mean oxygen radical toxicity, and the decreased glutathione peroxidase and catalase activities may play a role in cellular injury.

Study of national laboratory animal program on out-of-the way laboratory animal resources

Chuel kyu Kim,Dae Youn Hwang,Byoung Guk Kim,Yong Kyu Kim,Sun Bo Shim,Seung Wan Jee,Su Hae Lee,Ji Soon Sin,Chang Jun Bae,Byoung Chun Lee,Jung Sik Cho,Kap Ryong Chae,Hyoung Jin Kim,Byoung Chue Kim 한국실험동물학회 2007 한국실험동물학회 학술발표대회 논문집 Vol.2007 No.-

Survey on Current Status of Out-of-the Way Laboratory Animal Resources for Establishing National Policy

Chuel Kyu Kim,Dae Youn Hwang,Byoung Guk Kim,Seung Wan Jee,Yong Kyu Kim,Sun Bo Shim,Su Hae Lee,Ji Soon Sin,Chang Jun Bae,Byoung Chun Lee,Jin Hee Park,Se Heon Lee,Jung Sik Cho,Hyoung Jin Kim,Byoung Cheo 한국실험동물학회 2007 Laboratory Animal Research Vol.23 No.3

It has been required to manage the laboratory animals with systemic and scientific manner and to develop the animal models for human diseases, according to the development of new drugs based on the research of gene function and irredeemable diseases activated by National Dynamic Project of 21th Century on Life and Health. It is also necessary to accredit with high quality of laboratory animals for evaluating the safety and validity of foods and drugs, which are directly connected to human health. The aim of this study was to search current status on genetically engineered animals in order to enhance the scientific trusts of these animals to advanced level, and thus providing the book guiding us how to manage and breed the genetically engineered animals. To accomplish this, 107 researchers were selected to ask for current status of genetic engineered animals to 35 questions including the area of management, patent, and law-regulation. We concluded that (ⅰ) there are the total of 146 genetically engineered animals and the total of 29 patents in the country, (ⅱ) the 64 different embryos from mouse, pig, dog, and wolf are stocked, and (ⅲ) it is being prepared to publish a guide book on the basis of these results. Thus, these results raised a possibility that guide book provides a good opportunity to experts acting in the field of evaluating toxicants and of testing drugs as a guideline. It is also possible to use as fundamental materials to establish national policy on the laboratory and genetic engineered animals, and to generate Laboratory Animal Law, which is being review by members of National Assembly.

Survey on Current Status of Laboratory Animal Quality control in GLP institutions for Establishing a National Policy in Korea

Byoung Chun Lee,Mee Kyoung Jang,Young Gil Wang,Chang Jun Bae,Ji Soon Sin,Su Hae Lee,Seung Wan Jee,Sun Bo Shim,Byoung Guk Kim,Dae Youn Hwang,Jung Sik Cho,Kap Ryong Chae,Cheul kyu Kim 한국실험동물학회 2007 한국실험동물학회 학술발표대회 논문집 Vol.2007 No.-

일본뇌염 백신의 역가시험용 국가 표준품 확립을 위한 기반 연구

김재옥,김재근,백선영,신진호,민경일,민복순,류승렬,김병국,김도근,안미진,박미경,송혜원,이석호,박순희 식품의약품안전청 2000 식품의약품안전청 연보 Vol.4 No.-

일본뇌염은 모기에 의해 전염되는 질병으로 전 세계적으로 매년 오만명의 환자를 발생시키며, 생존자의 1/3 이상에서 신경계 합병증에 의한 장애를 초래하는 것으로 추측되고 있다. 이러한 일본뇌염의 예방책은 모기에 의한 전파를 차단하거나 예방백신을 접종하는 것인페, 특히 예방절종의 경우 그 효능 성이 이미 일븐, 한국, 대만 등a'i에서 입증된 바 있다. 일본뇌염백신의 역가를 검정하는 방법으로는 PRNT 방법을 통하여 대상 백신을 표준품과 hi교하는 방법이 널리 사용되고있는페 우리나라의 경우 현재 표준품이 없어 일본의 료준품을 전량 고가로 수입하고 있다. 따라서 국내 표준품을 확립하기 위한 기반 연구를 실행하게 되었다. 극십자백신(주)로부터 표준품 후보물질을 제공받아 국내에서 일본뇌염백신을 제조하는 5사와 공둥연구를 하였으며, 실헝 결과 표준품 후보물질이 표준 대조물질(일본 표준품)보다 약간 높은 중화항체가를 나타내었다. 실험실별로 4회 실험의 반복성을 통계 분석한 결과 B실험실의 표준 대조물질을 제외하고는 윤의수준 o=0.05에저 차이가 없는 것으로 나타났으며, 6개 실험실간의 쵸준 표준물질과 표준품 후 보물질의 시험결과를 분석하였을 때는 모두 변이계수에 있어 차이가 있는 것으로 나타났다(p<0.05). 이는 다른 실험실에 비해 실험실 ID쓱 I의 시험결과가 낮기 때문으로 추정되떠, WHO의 자료에 따르면 표준품을 설정하기 위해서는 최쏘한 6회 이상의 시험을 권고하고 있어 향후 더 많은 실험 및 결과 분석이 뒤따라야 할 것으로 생각된다. Japanese encephalitis (JE) Is a viral disease which is t37ansmitted by mosquitos and it affects aboi?t 50,000 people in Asia per year Lfore than one third of survivors from thehfection are suffering from adverse events related with neurological disorders. As a preventingmeasure of the asease, vacci·lation using inactivated JE vaccine has already been proven forits efficacy in Japan, Korea and Taiwan. To evaluate a potency of inactiyated JE vaccine, plaque reduction neutralization test (PRN17)was used to compare the test material to its standard. Since there is no national referencemade in Korea, 』γe have used reference material purchased from Japan. It is, however, difficultto obtain the reference material with sufficient quantity and a reasonable price. The collaborative study was performed according to standard opel·ating procedure of fEpotency test provided b? KFDA. The results were analyzed for calibration by paralle)experiment using reference and reference candidate. To solve this difficult situations, we have performed a collaborative study to make Koreanational reference of JE vaccine. National reference candidate was provided by Green CrossVaccine Compan17 A total of six laboratories including five JE vaccine manufacturers'and anational control laborato?F participated in this coltaborative study. As a result, national reference candidate shows a little higher neutralization titer than that ofJapan reference. There is no significant difference of repeatability in each laboFatorY except one.According to WHO docur)ent, its four separate potency tests were not enough and at least t)Yomore experiments are reauired.

Comparison of sirolimus-eluting stent and paclitaxel-eluting stent for long-term cardiac adverse events in diabetic patients: The Korean multicenter angioplasty team (KOMATE) registry

Kim, Jung-Sun,Lee, Byoung Ho,Ko, Young-Guk,Choi, Donghoon,Jang, Yangsoo,Min, Pil-Ki,Yoon, Young-Won,Hong, Bum Kee,Kwon, Hyuck Moon,Ahn, Min-Soo,Lee, Seung-Hwan,Yoon, Jung Han,Lee, Byoung Kwon,Kim, Byu Wiley Subscription Services, Inc., A Wiley Company 2008 Catheterization and cardiovascular interventions Vol.72 No.5

<P>Background: There is some controversy on long-term cardiac outcomes between sirolimus-eluting stents (SES) and paclitaxel-eluting stents (PES) in diabetes mellitus (DM). We compared cardiac adverse events after SES and PES implantation in patients with DM over a period of 3 year. Methods: A total of 634 patients with DM treated with SES (n = 428) or PES (n = 206) were consecutively enrolled in the KOMATE registry from 2003 to 2004. We assessed major adverse cardiac events (MACEs, cardiovascular death, nonfatal myocardial infarction, ischemia driven target vessel revascularization) and stent thrombosis (ST) according to the definitions set by the Academic Research Consortium. Results: Propensity score (PS) analysis was performed to adjust different baseline characteristics. The mean follow-up duration was 38 ± 8 month (at least 36 month and up to 53 month). The 3-year MACE rate did not show a significant difference between the two groups [52 (12.1%) in SES vs. 29 (14.1%) in PES, P = 0.496]. The definite and probable ST at 3 year were similar in both SES and PES [12 (2.8%) in SES vs. 7 (3.4%) in PES, P = 0.681]. There were no differences in hazard ratio for MACE and ST between two stents [MACE, crude: 0.844 (0.536–1.330) and adjusted for PS: 0.858 (0.530–1.389); ST, crude: 0.820 (0.323–2.083) and adjusted for PS: 0.960 (0.357–2.587)]. Conclusions: The present study demonstrated that long-tem cardiac outcomes including ST were not significantly different between SES and PES in patients with DM. © 2008 Wiley-Liss, Inc.</P>

Serial changes of minimal stent malapposition not detected by intravascular ultrasound: follow-up optical coherence tomography study

Kim, Won Ho,Lee, Byoung Kwon,Lee, Sahng,Shim, Jae-Min,Kim, Jung-Sun,Kim, Byoung-Keuk,Ko, Young-Guk,Choi, Donghoon,Jang, Yangsoo,Hong, Myeong-Ki Springer-Verlag 2010 Clinical research in cardiology Vol.99 No.10