녹동균 세포외막 단백질 백신 CFC-1-101의 안정성 및 면역원성 검토 : 임상 제 Ⅰ/Ⅱa상 시험

장인진,김익상,유경상,임동석,김형기,신상구,장우현,박완제,이나경,정상보,안동호,조양제,안보영,이윤하,김영지,남성우,김현수 대한감염학회 1998 감염 Vol.30 No.3

목적 : 제일제당에서는 녹농균의 세포외막 단밸질을 유효성분으로 하는 백신인 CFC-101을 개발하였으며, 동물시험에서 이 백신의 안전성과 유효성을 입증하였다. 본 연구에서는 이 녹농균 백신의 인체에 대한 안전성과 면역원성을 평가하는 동시에 인체 접종시의 최적 투여 용량을 결정하기 위하여 제 I/Ⅱa상 임상시험을 수행하였다. 방법 : 건강한 성인 남자를 피험자로 선별하여 각 용량군에 백신투여자 6명, 위약투여자 2명을 배정하였다. 백신 투여군은 0.25mg, 0.5mg 또는 1.0mg 용량의 녹농균 백신을 7일 간격으로 3회에 걸쳐 근육주사 하였으며, 위약 투여군에게는 세포외막 단백질을 제외한 동일한 성분을 투여하였다. 백신접종 후 국소적 또는 전신적인 반응의 발생여부를 관찰하고, 혈액시료를 체취하여 백신의 역가와 유효성을 검정하였다. 결과 : 녹농균 백신 CFC-101은 모든 접종자에서 양호한 내약성을 보였다. 또한 0.5mg 과 1.0mg 백신 투여군에서는 100%의 항체양전율을 나타내었다. 생성된 항체는 녹농균 세포외막단백질에 특이성을 보였고, 녹농균 감염에 대해 방어효능이 있었다. 결론 : 이와같은 결과로부터 이 녹농균 백신은 인체에 안전하게 투여할 수 있으며, 높은 항체 생성능으로 감염방어 효능을 보이고 0.5mg과 1.0mg이 최적용량인 것으로 판단되었다. Background : We developed a Pseudomonas aeruginosa outer membrane protein(OMP) vaccine CFC-101, and the prophylactic efficacy of which has been demonstrated in animal models. In order to evaluate the safety and immunogenicity of the P. aeruginosa vaccine, we carried out a phase I/Ⅱa clinical trial in healthy male volunteers. Methods : Groups of eight volunteers, including two placebo subjects, were vaccinated intramuscularly with three doses of 0.25, 0.5 or 1.0 mg of the vaccine at one week intervals. Sings of systemic and local reactions observed after vaccination were recorded for each vaccinee for 5 days. Physical examinations were performed on days 0, 1, 7, 8, 14, 15, 21, and 42, and clinical laboratory tests were done on days 0, 3, and 21. Blood samples for assay of serum antibody levels were obtained up to 42 days after the first vaccination. Results : The vaccine was generally well tolerated by all vaccinees, showing no significant side effects. In the three dosage groups, all vaccinees, except one receiving the 0.25 mg dose, showed significant elevation in serum IgG antibody titers against the vaccine proteins, indicating 100% seroconversion in 0.5 and 1.0 mg groups. The human antibodies induced by the vaccine were specific for P. aeruginosa OMPs, as confirmed by western blot analysis and immunoprecipitation assays. The capacity of the human antisera to enhance opsonophagocytic killing activity by polymorphonuclear leukocytes and to confer protection against P. aeruginosa infections indicates that the antibodies elicited by the vaccine have protective efficacy. Conclusion : We conclude that the P. aeruginosa OMP vaccine is safe and effective for human use and its optimal dose to be 0.5 or 1.0 mg.

고두께 유리-실리콘의 대면적 양극접합에 관한 연구

김건년,이보나,박효덕,신상모,이근혁,권혁채 경북대학교 센서기술연구소 1998 센서技術學術大會論文集 Vol.9 No.1

In this paper, we studied the anodic bonding of 5-inch silicon and #7740 Pyrex glass wafers with a thickness of 3mm by using the EV501 bonder and the Karl Suss SB6 bonder. The test conditions for anodic bonding of the EV501 bonder system with a full field electrode were temperature of 400 ℃ and voltage of 800V in a chamber pressure of 1X10^(3)mbar. The SB6 bonder with a star shaped electrode was tested at the temperature and voltage of 450 ℃ and 1300V in the atmosphere, respectively. As the results of test, we obtained the void free samples regardless of shape of substrates such as etched wafers with cavities and drilled glasses with holes.

Si Bulk Micromachining을 위한 Wafer Rolling Etching 및 그 특성

김건년,이보나,박효덕,신상모,공경준,장동근,김병철,권혁채,이봉희 경북대학교 센서기술연구소 1998 센서技術學術大會論文集 Vol.9 No.1

A wafer rolling etching system for the silicon bulk micromachining has been designed and fabricated. The silicon diaphragms were anisotropically etched in a 24.5 weight percent KOH solution. Compared to the conventional KOH etching systems, pyramidal hillocks, and wave-shaped structures on the etched surfaces were greatly reduced by using this system. After etching for time of 438 minutes, the average etched depth and the etch-rate were measured to be 537μm and 1.22μm/min, respectively. The average etching uniformity of etching depth was 0.87% in 5-inch wafer. Our results showed that the wafer rolling method enhanced etch uniformity and etch rate.

인장력을 받는 ㄷ형강 고력볼트 접합부의 감소계수 U값에 관한 연구

김진숙,표영석,나승욱,신영록,김보영,최문식 대한건축학회 2003 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.23 No.2(구조계)

The purpose of this paper is to examine the influence of reduction coefficient according to the limit state design of steel structures in the bolted joints of tension members. It was carried out the tensile testing about high strength bolted connections performed in structural steel channels. Considered parameters are the number and row of bolts, the connection length, connection eccentricity of the specimen. The test results showed that failures generally came (a) classic net section, (b) block shear failures. We found that the professional factor, obtained by calculating the test failure load divided by the nominal specification strength, is lower than 1.00. We thought that the reason was the moment produced by eccentric loading depends upon the connection geometry as well as the rotational stiffness of the connection.

집적화된 실리콘 압력센서의 출력전압 보상파라미터 추출 및 그 특성

이보나,김건년,박효덕,신상모,이경탁,김찬,권혁채,이상조,박현주 경북대학교 센서기술연구소 1998 센서技術學術大會論文集 Vol.9 No.1

An integrated silicon pressure sensor has been designed, fabricated and tested. The signal conditioning circuits were designed to include calibration and temperature compensation of output voltage through trimming of diffusion and ion-implanted resistors. Before trimming of resistors, the compensation parameters such as pressure sensitivity, temperature coefficient of pressure sensitivity, temperature coefficient of piezoresistors and pressure sensitivity of piezoresistors were measured. Then offset voltage, span, and temperature coefficients of offset voltage and span were calibrated by trimming of resistors. The measured output voltage met our design specification and simulation value above room temperature. But, the measured output voltage at -30°C deviated from our design specification and simulation value because the offset voltages were found to vary randomly as a function of temperature.

한약제형 선호도에 관한 설문조사

최현정,방나영,송보완,김남재,류봉하 WHO COLLABORATING CENTRE FOR TRADITIONAL MEDICINE 2004 東西醫學硏究所 論文集 Vol.2004 No.-

In order to investigate to develop the new dosage form of the traditional herbal medicine which have been used for the treatment of disease in oriental medicine, we conducted a survey on 217 patients who visited oriental hospital in Kyung Hee University Medical Centre during one month since October 2003. They were given questionnaires to answer and results of this study were as the following: 1. About 45% patients visited in oriental hospital believed that oriental medicine was better remedy than modern medicine. 2. Most of patients felt that the oriental medical expenses were expensive. 3. About 60% patients preferred the modernized dosage form such as extract powder and granule, etc. rather than medicinal herbs in package. 4. Most of patients hope that decoction of medicinal herbs in package would change the advanced dosage forms such as tablet, capsule and extract powder, Because patients suffered from inconvenience of making a decoction, unhandy, unwieldy and keeping. 5. About 60% patients pointed out that merits of advanced dosage forms of medicinal herbs were convience to take and handy, etc.. However, they had an Intension that there were some difference of efficacy between decoction and modernized dosage forms of oriental medicine. Because of several defects such as unusual flavor and smell, incompatible food, large volume with amount, inconvenience of carrying and decocting oriental herbal medicines, most of patients would expect the development of the advanced dosage forms of them. In summary, this study show that it was needed to develop the new dosage forms of oriental herbal medicine on the basis of scientific research and economical cost of medical expenses.

서울시내 고등학교 위탁급식의 급식비와 투자비의 실태 및 위탁업체의 기대수준 비교 분석

양일선,배환미,김현아,신서영,조미나,박수연,차진아,이보숙 한국식생활문화학회 2002 韓國食生活文化學會誌 Vol.17 No.5

The purposes of this study were to a) find out the operational characteristics of the contract-managed highschool foodservice in Seoul, b) investigate the expected level of meal-price and facilities investment cost perceived by contract-managed highschool foodservice managers c) compare the present level and expected level of meal-price and facilities investment cost. From October 12 to November 13 in 2001, the questionnaires were mailed to 249 high schools which was managed by contract foodservice company with respondent rate 40.2%. Data were analyzed using SPSS Win(10.0) for descriptive analysis and one group paired t-test. The results of this study were as follows ; 1. The student enrollment of highschools run by contract-managed foodservice was 1,243 with 72.6% participation rate of school lunch program. The average meal-price was 2,138 won. The average annual period of school foodservice operation was 156.78 days per year. The average contracting period was about 3 years. 2. The average cost concerned in the facilities investment amounts 169,578,180 won at the initial investment and 25,204,092 won at the repairs and maintenance cost in the course of operation. 3. The present level of meal-price and facilities investment cost were respectively 2,136won/meal and 171,157,336.72 won. And expected level.of meal-price and amount of facilities investment cost were 2,418.75 won and 121,353,215.19 won. Comparing the present level with expected level of the meal-price and facilities investment cost, expected level of meal-price was significantly higher than the present level of meal-price(p<.001) and expected level of facilities investment cost was significantly lower than present level of facilities investment cost(p<.001).

인장력을 받는 ㄱ형강 접합부의 감소계수 U값에 관한 연구

표영석,윤성호,나승욱,신영록,김보영,최문식 대한건축학회 2003 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.23 No.2(구조계)

This study focuses on the inspect of effective net area design code, according to limited state design criteria of steel structures, recently established in Korea, by an experiment on the joint of angle tension members on the ground. reduction factor. The methods of this study were to compare other study results on effective net area rupture mode and ultimate capacity, and to evaluate the propriety of the criteria design code. The result is that code in force limited state design criteria of steel structures and AISC-LRFD joint of angle tension members predicted load and experiment depend on ultimate capacity difference. Therefore, it is thought that there is need for supplemental test and study to effective net area of tension joint focused on bolted connection of angle.

대한약전 수재 의약품의 기기분석법에 관한 연구(ⅩⅡ) : 고속액체크로마토그래프를 이용한 아크리놀 및 그 제제의 분석법에 관한 연구 Studies on analytical Methods of actinol and its preparations by HPLC

이석호,옥치석,최보경,김영옥,최리나,박상애,최명신,김길수,이강춘 식품의약품안전청 1997 식품의약품안전청 연보 Vol.1 No.-

아크리놀은 살균,소독에 쓰이는 약물로서 연로제 및 지사제용도의 경구투여제제등게 이용되고 있다. 공정서에 수재된 아크리놀의 분석법은 적정법이고 제제에서늬 분석법은 확립되지 않았고 원료의 적정법을 제제에 적용시 다른 주성분이나 부형제 또는 안정제 드으이 영향으로 분석오차가 생길 우려가 있다. 따라서 아크리놀을 보다 신속·정확하게 확인 및 정량분씬을 할 수 있고 강도와 분리능이 우수한 HPLC를 이용하여 아크리놀을 분석하고자 하였다. MPLC 분석조건은 이동상 :아세료니트릴 · 1% 인산일수소나트륨혼합액(40:60)(p템 5.2), 칼랏 :옥타데실실릴화한 실리카칼럼,검출기 :자외부흡광광도계(측정파장 : 254nm)을 사용하여 양호한 결과를 얻었다. 틴PLC법의 분석 결과, 검량선의 상관계수는 0.9999, 재현성(aSD(%))은 0.5234. 정균회수율은 100.28%(RSD=0.89%)로 정량성 및 재현성이 높온 분석 결과를·얻었다. 또한 시판품에 대하여 려PLC분석법과 공정서에 수재된 적정법의 시칩결과를 비교하였을 때 핀Plf법인 더욱 정확한 결과를 얻을 수 있었으므로, 조작이 간편하고 재현성이 높은 딘PLC분석법을 약전차정의 기초자료로 활용하고자 한다. We describe a method for the determination of acrino1 and its preparations by HPLC. The optimum analrtical coriditions were as follows;column : u-Bondapak C_(18) mobile phase : acetonitrile 1% Na_(2)HPO_(4), buffer (40:60)(pH 5.2), detector : 254nm, flow rate 1.OmL/min, injection volumn : 5uL, The calibration curve at 254nm was linear within the range of 10 ~ 140㎍/mL. This HPLC method has been validated to be precise, accurate and linear. This method has also been found to be applicable to other formulations containing acrinol.

New Records of Host Plant for Radopholus similis Isolated from Agathis dammara

Na-Ri Lee,Dong Woo Kim,Bo-ram Kim,Bok-ri Park,Jae yong Chun 한국응용곤충학회 2014 한국응용곤충학회 학술대회논문집 Vol.2014 No.04

The aim of the study is to report Radopholus similis detected from Agathis dammara in Thailand as new host plant. Existing host of R. similis was known as coffee, pepper, sugarcane and banana etc. This nematode in this study was observed morphologic character using Carl Zeiss Axioimager M2 and Axiovision Rel. 4.8 program. The result shows that a and b of the female was distorted toward maximum value of original description of species, and b′, c and stylet length was distorted toward minimum value of original description of species. This nematode was morphologically distinguished from originally detected R. similis, but measured value was similar in range of original description of species. For more information, molecular assay was also confirmed the R. similis with 98% homology with the sequence of the internal transcribed spacer region (ITS) of ribosomal DNA. We provided PCR-amplified ITS nucleotide sequence.