건강한 청년의 단시간 공해노출과 일회성 운동이 폐기능과 생리반응에 미치는 영향

김민주(Min-Joo Kim),이대택(Dae-Taek Lee),송국섭(Gook-Sup Song),방승기(Seung-Ki Pang) 한국생활환경학회 2015 한국생활환경학회지 Vol.22 No.1

This study examined whether an acute exercise during a short-term exposure to a polluted environment affected pulmonary function and physiological responses in healthy young adults. Thirteen men (mean = 22.4 yrs) were randomly exposed to a polluted air (PA) or to clean air (CA) in a chamber. During the exposure, they rested for 30 min followed by a bench stepping exercise (40 cm height, 120 bpm) for 10 min. And they recovered from the exercise for 15 min in the chamber. The pulmonary function, heart rate, blood lactate, and blood pressure were measured during the exposure. At the end of 30 min resting, peak expiratory flow rate (PEFR) was higher in PA than CA. Immediately after the exercise, forced expiratory volume at 1 sec (FEV1) was higher in PA than CA. At 15th min of recovery, forced vital capacity, FEV1, and PEFR were significantly higher in PA than CA. No differences in cardiovascular and blood lactate responses were noticed. The results suggest that an acute intensive physical work out with breathing of volatile organic compounds induced changes of pulmonary function but not physiological adjustments.

대장내시경검사의 전처치로서 항콜린제 사용에 관한 연구 : 전향적, 이중맹검법으로

박경남,한동수,이민호,최호순,박준용,손주현,이오영,함준수,전용철,송승찬,기춘석,윤병철,이종희 대한소화기내시경학회 1997 Clinical Endoscopy Vol.17 No.3

Background: Use of antispasmodic medication prior to colonoscopy is controversial but someone believes antispasmodic may improve visualization of colonic mucosa and ease colonoscope insertion. So, we designed a study to assess the effect of premedication with the antispasmodic, hyoscine-N-butyl bromide(Buscopan') on the performance of colonoscopy. Methods: This study was prospective, double blinded, randomized, controlled study, One hundred three consecutive patients were randomized to receive intravenous buscopan lml(n=52) or placebo(n=51) combined with our standard initial medication(me- peridine 50 mg and midazolam 2 mg). Insertion of colonoscopy was timed, and 100 mm visual analogue scales (VAS) were used for asscssing difficulty of procedure, colonic motility, frequency of positional change, frequency of external compression, difficulty of assistance and degree of discomfort experienced by the patients. Results: There were no significant differences of intubation time between buscopan group(mean time, 7.23 min., range 2~15) and placebo group(7.07 min., range 3-25), (p=0.83) and withdrawal time between buscopan group (6.46 min., range 2-22) and placebo group(6.76 min., range 2 25), (p=0.69). Also, there was no significant differences in intubation time between males and females(buscopan; males 7.00 min., females 7.60 min., p=0.34, placebo; males 7.0~5 min., females 7.08 min., p 0.44). The VAS scores checked by endoscopist(p=0.29), assistant(p=0.32) and patient (p=0.15) were not significantly different in both groups. There were no significant differences in intubation time, VAS scores nf endoscopist, assistant, and patients. Conclusion: Premedication with intravenous bu.opan has no advantage on colonoscopy procedure. Use of antispasmodic medication prior to colonoscopy was not considered as recommendable agent.

Effects of hydrogen plasma treatment on SnO2:F substrates for amorphous Si thin film solar cells

Min-Seung Choi,Young-Ju Lee,Jung-Dae Kwon,Yongsoo Jeong,Ju-Yun Park,강용철,Pung Keun Song,Dong-Ho Kim 한국물리학회 2013 Current Applied Physics Vol.13 No.8

We investigated the effects of hydrogen plasma treatment on the physical and electrical properties of fluorine-doped tin oxide (FTO) films used for amorphous silicon (a-Si) thin film solar cells. A slight increase in carrier concentration by the hydrogen doping effect was observed for the FTO film exposed to the hydrogen plasma for 5 min. For further exposure to the plasma, the chemical reduction became prominent and resulted in deterioration of the electrical and optical properties of the film. XPS analysis revealed that the chemical reduction of SnO2 to Sn metallic state occurs on the surface region. It was found that the defects formed by hydrogen plasma act as recombination centers at the interface between FTO electrode and p-layer of a-Si solar cells. This phenomenon resulted in the deterioration of the cell performance. The averaged conversion efficiency (6.82%) of the cells on pristine FTO hydrogen substrate was decreased to 5.81% for the cells on FTO treated for 5 min, which is mainly attributed to the decrease in short-circuit current density.

안와골절의 외과적 접근에 대한 증례보고

민승기,이은택,오승환,이동근,고세욱,송종민,최성림 대한악안면성형재건외과학회 2002 Maxillofacial Plastic Reconstructive Surgery Vol.24 No.3

The Orbital fracture which is often combined with midface fracture can cause decreased visual acuity, limitation of eyeball movement, diplopia, enophthalmos, etc. Traumatic orbital fracture causes change of orbital volume, results in diplopia and enophthalmos thus, accurate repositioning of displaced bone and reconstruction of orbital defect with autogenous materials(cranial, rib, iliac bone, cartilage and fascia lata) or alloplastic materials(gelatin film, polyglactin mesh, methylmethacrylate, Teflon, silicone, Supermid, hydroxyapatite and metal). The key point of reconstruction of orbit is accurate repositioning of displaced orbital floor, lateral, medial orbital wall and sufficient bonegraft in anatomical defect. As this cases, we obtained good results through transconjunctival and coronal approach who were required orbital reconstruction. Also, we accurately diagnosed orbital fracture with C.T., 3D model and good result for orbital reconstruction with sufficient parietal block bone graft during average 16.8 months follow-up.

Purification and Characterization of Helicobacter pylori ${\gamma}$-Glutamyltranspeptidase

Song, Jae-Young,Choi, Yeo-Jeong,Kim, Jeong-Min,Kim, Yoo-Ree,Jo, Jin-Seong,Park, Jin-Sik,Park, Hee-Jin,Song, Yun-Gyu,Lee, Kon-Ho,Kang, Hyung-Lyun,Baik, Seung-Chul,Youn, Hee-Shang,Cho, Myung-Je,Rhee, Kw The Korean Society for Microbiology 2011 Journal of Bacteriology and Virology Vol.41 No.4

Gamma-glutamyltranspeptidase (GGT) was purified to electrophoretic homogeneity from the cell extract of H. pylori. The purified enzyme consisted of heavy and light subunits with molecular weights of 38 kDa and 21 kDa, respectively. N-terminal amino acid sequence of heavy and light subunits revealed that H. pylori GGT was processed into 3 parts for a signal peptide of 27 amino acid residues, a heavy subunit of 352 residues, and a light subunit of 188 residues during translation. The reaction rate for hydrolysis of ${\gamma}$-GpNA was 84.4 ${\mu}mol/min$ per milligram of protein, and that for the ${\gamma}$-glutamyl transfer from ${\gamma}$-GpNA to gly-gly was 23.8 ${\mu}mol/min$ per milligram of protein. The apparent Km values of H. pylori GGT for ${\gamma}$-glutamyl compounds were on the order of $10^{-3}$ to $10^{-4}$ M and those for acceptor peptides and amino acids were on the order of $10^{-1}$ to $10^{-2}$ M. The GGT protein kept approximately 80% of the initial enzymatic activity on incubation at $60^{\circ}C$ for 15 min. The optimum temperature and pH for reactions of both hydrolysis and transpeptidation were $40^{\circ}C$ and 9.0, respectively. The transpeptidation and hydrolysis reactions catalyzed by H. pylori GGT were strongly inhibited by L-Gln and moderately inhibited by L-Ala, L-Ser, ${\beta}$-chloro-L-Ala, and L-Glu. These results demonstrated that the biochemical properties of H. pylori GGT are different from those of other bacterial GGTs. Further, H. pylori GGT might degrade glutathione in the gastric mucous layer of humans if the enzyme could be secreted in the bacterial niches.

실내 스모그 챔버의 설계 및 성능평가

배귀남,김민철,이승복,송기범,진현철,문길주 한국대기환경학회 2003 한국대기환경학회지 Vol.19 No.4

A multi-functional indoor smog chamber was designed and evaluated lo investigate photochenical or water vapor reaction mechanisms of air pollutants. Various smog chamber experiments could be conducted using ambient air or purified air in this smog chamber. The smog chamber consisted of a housing, a Teflon bag, blacklights, injection ports, sampling ports, and utility facilities. The characteristics of light source, the wall losses of air pollutants, and the quality of purified air were experimentally investigated. The maximum NO₂ photolysis rate was 1.10min^(-1). In a 2.5-m³ Teflon bag, the wall losses of ambient O₃, NO, and NO₂were 1.2~2.4×10^(-3)min^(-1), 0.7~2.0×10^(-3)min^(-1), and 0.4-2.0×10^(-3)min^(-1), respectively. The wall loss of ambient particles ranging 0.05 to 0.2㎛ was 1.8~5.4×10^(-3)min^(-1), which was slightly higher than those of ambient gaseous species. The purified air supply system provided high quality of air with NO₂, < 1 ppb, and total hydrocarbons <5 ppb.

온풍 조건에서 수분 탈락 정도에 따른 피부 분류 및 개선 방안에 대한 연구

권오선 ( Oh Sun Kwon ),강현종 ( Hyun Jong Kang ),한승민 ( Seung Min Han ),윤지선 ( Ji S Eon Yoon ),조웅희 ( Woong Hee Cho ),오주영 ( Joo Young Oh ),임준만 ( Jun Man Lim ),송영숙 ( Young Sook Song ),박선규 ( Sun Gyoo Park ) 대한화장품학회 2020 대한화장품학회지 Vol.46 No.2

피부를 탄력있고 부드럽게 하는 역할은 각질층에 존재하는 수분량에 의해 좌우된다. 피부 수분량은 냉온풍, 건조환경 등 다양한 환경 변화에 의해 영향을 받음이 알려져 있으나, 개인 피부 차이에 따른 피부수분량 변화와 회복 정도에 대해서는 많은 연구가 이루어 지지 않은 실정이다. 본 연구에서는 온풍 조건하에서 피시험자들의 피부 수분 탈락 및 회복 정도를 비교 평가하여 새로운 피부 타입을 제시하고, 온풍 조건에서 저하되는 피부 수분량을 개선 시켜주는 효능 물질을 개발하고자 하였다. 온풍 환경 조성을 위해, 건강한 피험자(남: 10 명, 여: 39 명, 25 세 - 63 세)의 전완부에 온풍(30 cm, 40 ℃, 6 m/s)을 30 min 간 피부에 노출시켜, 피부 수분량의 변화를 평가하였다. 26명(남: 4 명, 여: 22 명, 평균 연령: 42.7 ± 9.4)이 온풍 노출전에 비하여 온풍 노출 후 수분량이 유의하게 감소하며, 노출 후 30 min이 지나도 회복이 되지 않았다. 온풍노출 후 수분량이 떨어지는 피험자(여: 10 명)를 대상으로 보수력이 높은 크림을 3 주간 전완부에 적용한 이후 동일 온풍 조건하에서 피부 수분량을 측정한 결과, 노출 30 min 후 피부 수분량이 온풍 노출 전 수준으로 회복됨을 확인하였다. 본 연구를 통하여 피부는 건조 조건에서 쉽게 수분을 잃어버리는 피부(탈수형 피부)가 존재하는 것을 확인하였다. 이는 앞으로 화장품 개발을 보습 기능뿐만 아니라, 이러한 환경변화에 따른 피부수분이 쉽게 빠져나가는 피부(탈수형 피부)의 특성에 맞는 제품의 효능 개발이 필요함을 보여준다. Elasticity and softness of the skin depend on the level of moisture present in the stratum corneum, which is known to be affected by various environmental changes, such as cold and hot winds and dry environments. However, not many studies have been conducted on changes in skin moisture and the degree of recovery due to individual skin differences. In the present study, we aimed to investigate the effect of warm air heating on skin hydration levels and develop moisturizing formulas to improve lowered skin hydration levels. In order to deliver warm air heating condition, heating dryer (40 ℃, 6 m/s, 30 cm apart from forearm) was applied into inner forearm of healthy subjects (male: 10, female: 39, age: 25 - 63) Among 49 subjects, 26 subjects showed significantly lowered skin hydration levels until 30 min after warm air heating exposure (lowered group). In addition, moisturizing cream with high water holding capacity was applied to forearm of 10 subjects in lowered group for 3 weeks and skin hydration levels after warm air heating were significantly improved at the levels of before application of warm air heating. From this study, we found out that there is a skin type that skin hydration levels are significantly decreased under warm air heating condition (dehydrated skin) and this dehydrated skin can be improved by moisturizing formulas with high water holding capacity.

생체분해성 고분자를 이용한 사시수술용 제형개발 및 효용성에 대한 연구

민병무,김용백,김승영,김창식,박근성,길숙종,조항진,이성복,노승무,송규상,강대영,조준식,양준묵,정경수,최선웅,이진호,김학용,인현빈 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.1

A new device, muscle clamping system was developed to facilitate exact quatifying technique A strabismus surgery, and reduce the risk of complications. The device is composed of a lower fixing body with three jaws and an upper supporting body. They are used to clip an extraocular muscle and fix it to the sclera with a single bite. Superior rectus recession on 16 rabbit eyes were performed with this new device. Conjunctival injection, muscle adhesion strength, and light microscopic findings were examined at 1, 2, 4 and 8 weeks postoperatively. The Conjunctival injection were minimal, adhesion power ranged from 420 to 600 gram gravity, which is sufficient in withstand the normal pull of human extraocular muscle. In microscopic exam, some Inflammatory cells and fibrosis were found. The new device was technically easy, fast, and accurate, so it may be useful in stabismus surgery.

백서의 복강내에 투여한 Mitomycin-C-Polyglycolic acid 제형이 장기에 미치는 영향에 관한 연구

송규상,강대영,최정목,노승무,정경수,오정연,김진향,양준목,최선웅,이진호,조준식,민병무,김용백,김창식,박근성,김승영,김학용,인현빈 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.1

To evaluate the in-vivo effects of mitomycin-C-polyglycolic acid composite(MMC-PGA) the disk: shaped composite were implanted into the peritoneal cavity of the Sprague-Dawley rats. The pathologic changes were examined at various time points up to 12 weeks. Initially the soft tissue around the inserted disks showed capillary congestion at 3 days. Inflammatory infiltrates with foreign body giant cells appeared from the 2nd week and reached peak response at 6-8 weeks. These reaction diminished prominently at 12 weeks. No specific pathologic change was found in the liver, the kidneys, and the heart. The above results suggest that the MMC-PGA composite can serve as a new device for intraperitoneal chemotherapy of various types of cancers.

급성기 가와사끼병 환아의 혈청 Vascular Endothelial Growth Factor 및 Endostatin에 대한 연구

송민섭,이승익 인제대학교 2008 仁濟醫學 Vol.29 No.-

Objective : Vascular Endothelial Growth Factor(VEGF) is an angiogenic growth factor that has been implicated in both physiological and pathological angiogenesis. Endostatin(ES) is a potent inhibitor of angiogenesis and exhibits antiangiogenic activity by inhibiting the proliferation and migration of endothelial cells(ECs) in addition to inducing endothelial cell apoptosis. Imbalance between angiogenic and antiangiogenic factor exists in subacute phase of KD, as pathologic angiogenesis occurs in subacute phase of Kawasaki disease(KD). The purpose of this study is to investigate whether an imbalance exists in the production between angiogenic and antiangiogenic growth factors in patients with acute phase of Kawasaki disease(KD), we measured the serum levels of VEGF and ES. Methods and Materials : Serum VEGF, ES levels and VEGF/ES ratio were measured in 30 KD patients, 10 incomplete KD patients and 12 febrile illness controls. Blood samples were drawn from each study group during acute phase before intravenous immunoglobulin(IVIG) therapy. Results : KD patients had higher VEGF levels(426.70 ± 479.63 pg/mL vs 297.71 ± 251.32 pg/mL, P=0.52) and VEGF/ES ratio(3.67 ± 5.69 vs 2.16 ±1.763, P=0.55 ) in the acute phase of KD than controls, but the difference was not significant. ES levels were within normal range and there was no difference of ES levels(132.65 ± 33.98 ng/mL vs 135.54 ± 25.95 ng/mL, P=0.97) between Kawasaki disease and controls in the acute phase. Conclusion : The findings in this present study indicate that an imbalance does not exist in the production between VEGF and ES in patients with acute phase of KD.