DJ-1 deficiency impairs synaptic vesicle endocytosis and reavailability at nerve terminals

Kyung, Jae Won,Kim, Jin-Mo,Lee, Wongyoung,Ha, Tae-Young,Cha, Seon-Heui,Chung, Kyung-Hwun,Choi, Dong-Joo,Jou, Ilo,Song, Woo Keun,Joe, Eun-Hye,Kim, Sung Hyun,Park, Sang Myun National Academy of Sciences 2018 Proceedings of the National Academy of Sciences Vol.115 No.7

<P>Mutations in DJ-1 (PARK7) are a known cause of early-onset autosomal recessive Parkinson's disease (PD). Accumulating evidence indicates that abnormalities of synaptic vesicle trafficking underlie the pathophysiological mechanism of PD. In the present study, we explored whether DJ-1 is involved in CNS synaptic function. DJ-1 deficiency impaired synaptic vesicle endocytosis and reavailability without inducing structural alterations in synapses. Familial mutants of DJ-1 (M26I, E64D, and L166P) were unable to rescue defective endocytosis of synaptic vesicles, whereas WT DJ-1 expression completely restored endocytic function in DJ-1 KO neurons. The defective synaptic endocytosis shown in DJ-1 KO neurons may be attributable to alterations in membrane cholesterol level. Thus, DJ-1 appears essential for synaptic vesicle endocytosis and reavailability, and impairment of this function by familial mutants of DJ-1 may be related to the pathogenesis of PD.</P>

공무원들의 인식에 나타난 경관자원 특성 분석 연구

박선명(Park Sun-Myung),박선경(Park Sun-Kyung),김주현(Kim Joo-Hyun),김종하(Kim Jong-Ha),이정호(Lee Jung-Ho),최무혁(Choi Moo-Hyuck) 대한건축학회 2010 대한건축학회논문집 Vol.26 No.11

In this study, to set up landscaping plans, characteristics of landscape were extracted based on opportunity and weaknesses factors of landscape after carrying out a questionnaire targeting officers in 23 cities and counties in Kyung-buk province and the findings are as following. First, in analyzing condition, component and value of landscape based on opportunity and weaknesses factors, for opportunity factors, it was found that the condition of landscape was quantitatively great, the component of landscape was industrially great and the value of landscape was great in possibility of development. Also, for weaknesses factors, the 'quality' was superior in its condition, the industry was superior in its 'component' and the shortage of artificial resources was superior in its 'value'. Secondly, in the relation between factors consisting opportunity and weaknesses resources, firstly, it was found that industry, city and nature are superior for opportunity factors, and culture, traffic and population are superior for weaknesses factors. Next, in the relation between component and condition, 'qualitatively superior industry' and 'quantitatively superior industry' had the highest frequencies in opportunity factors and 'qualitatively outstanding city' and 'quantitatively preponderant industry' had the highest frequencies in weaknesses factors. Thirdly, in analyzing the importance of opportunity and weaknesses factors, for opportunity factors, qualitatively superior resource was a priority in the importance of condition and for weaknesses factors, it was qualitatively little greater than the quantity. In the importance of component, the industry was most superior in both of strength and weak points and in the importance of value, the shortage of artificial resources was a priority. Fourthly, in characteristics of landscape, internal characteristics were found stronger than external characteristics in each city and county. This means that there are more resources to strategize in landscape plans of individual city and county unit than other in metropolitan unit. Also, possibility of development, possession of complex resources and geographical adjoin were common points of their strategies and plans.

식품 기준·규격의 합리적 관리 ·운영에 관한 연구(Ⅰ) : 두부,식용유지, 전분의 분말상 원료에 관하여 Powdered marerials of Tofu, Edible oil, Starch

김희연,홍진환,박혜경,한상배,박종석,이은주,이정성,송경희,최은희,최영준,소경아,성영제,이주엽 식품의약품안전청 2000 식품의약품안전청 연보 Vol.4 No.-

본 연구는 분말상 원료(전분, 대두분)의 저장유통시 품질변화를 건전성과 안전성 측면에서 검토하여 식품의 기준 ·규격 설정f.」 합지적으로 반영하고자 하였다. 전분과 패두분의 이물을 검사하고, 2종 대두분(중국산, 미국산)을 ?0일간 IS'c와 30t에 저장하면서 성상, 수분, 산가, 과산화물가, 지방산 조성,아플라톡신 BB의 생성여부를 분석하였다. 중국산 대두분 30'』 저장군에서 50일 이후 곰팡이가 발생한 것 이외에 모든 시료에서 양호한 성상을 나타내었고, 전분과 대두닥 모든 시료에서 이물은 검출되지 않았다. 산가는 중국산과 미국산 시료간에 편차를 보여 증국산 30"C, 50일 저장시료에서 약 10배끙도 증가하였고, 과산화물가는 저장 90일후 15'E 저장군은 약 2.3배, 30'c 저장군은 3배정도 증가하였다. 대두분의 주요 지방산은 linoleic acidf18. 2), oleic acid(18 : 1), palmitic acid(16 : 0)이었으며, 3개월 저장에 따른 지방산 함량의 변화는 미미하였다. 아플라톡신 B₁은 모든 시료에서 검출되지 않았다. The objective of this study was to investigate auality changes of type raw materials(starch, soy flour) during various storage conditions. Starchflour(source of China, U.S.A) were slfred under two temperature(15'C, 30'c) forSensory evaluation, (o.reign material test, moisture content, acid value, peroxide ualue,composition, aflatorin Bi were analyzed. China soy flour(stor·ed 30'f) was contaminated by fungj on 50 days. Except for soy flour contardnated by fungi, sensory characteristics were not changed and foreign mater;als were notdetected. Acid value of China soy flour(stored at 30'C, 90 days) was about 10 times higherlevel before storage and acid value was more influenced by source(China, USA). Peroxide valuewas in proportion with the storage period slightly. The major fatty acid of soy flour waslinoleic acid(18 . 2), an(3 the change of fatty acid composition was not observed. Aflatoxlrl Blwas not detected.

급성기 뇌경색 환자에서 화열 관련 증상과 증후의 변화에 관한 연구

곽승혁,박수경,우수경,이은찬,박주영,정우상,문상관,조기호,조승연,박성욱,고창남,Kwak, Seung-hyuk,Park, Su-kyung,Woo, Su-kyung,Lee, Eun-chan,Park, Joo-young,Jung, Woo-sang,Moon, Sang-kwan,Cho, Ki-ho,Cho, Seung-yeon,Park, Sung-wook,Ko, Ch 대한중풍순환신경학회 2011 대한중풍.순환신경학회지 Vol.12 No.1

Objective : Fire and heat related symptoms and signs are considered common in acute stage of diseases. The purpose of this study is to evaluate the occurrences and changes of fire and heat related symptoms and signs in acute cerebral infarction patients. Method & subjects : 40 acute cerebral infarction patients hospitalized in Oriental medicine hospital, Kyung-Hee University, who had examined and diagnosed 2 or 3 times based on oriental medical diagnosis were selected. We chose 23 as fire and heat related symptoms and signs from 94 diagnostic articles, and we added all those scores together of each patient. We analysed the scores of fire and heat related symptoms and signs as the time passed, and depending on oriental medical diagnosis. Result : In acute cerebral infarction patients of this study, 4 of fire and heat related symptoms and signs were took 1st, 2nd, 4th and 8th places in most changeable 10 articles of total 94 articles. The mean score of fire and heat related symptoms and signs of all patients were decreased significantly over the 3 times of measurements. The 8 patients diagnosed as fire and heat diagnosis at visit1 were samely diagnosed as fire and heat diagnosis at visit2, and at visit3 5 patients of them except for 3 patients excluded between visit2 and visit3, were still diagnosed as fire and heat diagnosis. At all of 3 measuring times, the scores of fire and heat related symptoms and signs of fire and heat diagnosis group were higher than non-fire and heat diagnosis group. Conclusion : This study indicated that fire and heat related symptoms and signs were very changeable phenomenon in acute cerebral infarction patients. And they decreased as time goes on.

액상 실리카륨을 혼입한 콘크리트의 파괴특성

박제선,민창동,김태경,이주형 江原大學校 産業技術硏究所 1995 産業技術硏究 Vol.15 No.-

The experimental study is conducted to analyze the characteristics of concrete included silica fume. The workability of concrete is controled by the ratio of mixture, such as superplastcizer, based on the constant rates of sand and cement. It is concluded that the strength and the frectu Toughness of concrete with silica fume are very improved.

집먼지 진드기에 감작된 천식 환자에서 면역치료의 효과

박경원,이경은,조영주 이화여자대학교 의과대학 2004 EMJ (Ewha medical journal) Vol.27 No.2

Immunotherapy has been considered as secondary strategy of IgE mediated allergic disease. It decreases delayed airway inflammation and blocks irreversible airway change but many controversies still exist despite of its remarkable effect. The purpose of this study was to estimate clinical effectiveness of immunotherapy in patients with asthma sensitized to house dust mite and to evaluate related factors. Thirty asthmatic patients visiting allergy clinic of Mock-Dong hospital of Ewha woman university enrolled from March, 1996 to February. All patients were received the D. farinae and D. pteronyssinus standardized extract by a immunotherapy(IT) schedule and we measured clinical parameters. 1) Symptom-medication score was significantly improved after treatment (8.9±0.29 ; before IT vs. 3.4±0.20 ; after IT, p<0.05). 2) Methacholine challenge test demonstrated significant improvement (PC20 0.78± 0.09 before IT vs. 1.l8±0.17 after I year of IT, p<0.05). After we divided patients into two groups, group I that showed more than twice increasing in PC20 after I year IT and group Ⅱ that did not. There was no significant difference between the groups in pre-treatment PC20s (8.75±2.75mg/ml in group Ⅰ vs. 7.08±1.84mg/ml in group Ⅱ, p<0.05). 3) Skin prick test demonstrated no significant change (wheel flare ratio in D. farinae 2.44±0.24 before IT vs. 2.11±0.26 after IT, in D. pteronyssinus 2.33±0.29 before IT vs. 2.22±0.22 after IT, p>0.05). 4) There were no significant correlations for any combination among these three parameters (symptomatic improvement, change of PC20 and disease duration of asthma). 5) In view of side effects, no fatal systemic reaction occurred during treatment period. Only one patient experienced injection-related dyspnea and urticaria that were easily controlled by medication. This study demonstrated that patients with asthma sensitized to house dust mite benefit from specific immunotherapy through symptomatic improvement and reduced nonspecific bronchial hyperreactivity. As for side reactions immunotherapy might be relatively safe if allergen is carefully administered a controlled and supervised environment.

장시간 불가피한 공복(fasting)후 인체의 내분비 변화 : 삼풍백화점 붕괴 사고후 구조된 3인의 증례 THREE CASES IN COLLAPSE OF SAMPOONG DEPARTMENT STORE

박규남,황주일,박조현,오동렬,이원재,오승택,김세경,김인철 대한응급의학회 1995 대한응급의학회지 Vol.6 No.2

The interactions between nutrition and the endocrine system are more intimate and complex that previously thought. Nutrition modulates secretion and activity of many hormones, as part of the stress adaptation process. Fasting, in particular, produces a number of important changes in the endocrine system, with teleological purpose of helping the body tolerate the lack of food ingestion. These changes are therefore beneficial and protective, although at times they may not appear to be that way. The endocrine changes of fasting mediate the metabolic response. We must emphasize that these changes are completely reversible when appropriate nutrition is established. These changes are as followes: 1) decreased insulin and increased glucagon levels, 2) decreased thyroid effect, 3) decreased sympathetic activity, 4) hypothalamic hypogonadism, 5) decreased growth, 6) altered glucocorticoid secretion and metabolism, 7) impaired mineralocorticoid response, 8) decreased ADH secretion and effect. We experienced three cases of involuntary prolonged fasting after collapse of Sampoong department store. They were released from collapsed field after 11(case Ⅰ), 13(case Ⅱ), 17days(case Ⅲ) respectively. In each case, the endocrine changes of plasma levels were as followes: decrease of cortisol in case Ⅰ, increase of GH in case Ⅱ, decrease of free T3, T3, cortisol and increase of reverse T3, insulin in case Ⅲ. Each one did not show the same endocrine changes of plasma levels. But we think these changes of fasting are beneficial in metabolic response of human.

음악요법이 위내시경 대상자의 검사전 불안에 미치는 효과

박경숙,나명주 성인간호학회 2003 성인간호학회지 Vol.15 No.2

This study examined the effect of music therapy on the pre-exam of gastroscopy clients. Method: The research period is from June 17 2002 to August 30 2002, and the subjects were 60 individuals, 30 for the experimental and 30 for the control group among adult clients. The data were analyzed by using the SPSS program. Result: The results of this study were as follows: The score of the experimental group was lower than the score of the control group for the situational anxiety (t=-12.56, p=0.000) and systolic blood pressure (t=-3.97, p=0.000) before the examination was supported. The vital sign was partially supported. There was no significant difference(t=-1.05, p=0.297) in rhe diastolic blood pressure (t=-1.05, p=0.297) and pulse rate(t=-1.30, p=0.199) before the examination of the experimental group and lower than the score of the control group. Conclusion: Music therapy could be useful in the nursing practice, and be utilized as a way of nursing intervention to reduce anxiety of the patients in specific clinical situations. Finally, it is recommended that further studies be conducted on this therapy by utilizing different musical selections.

연안 도시 대기오염 물질의 농도분포 특성

박종길,석경하,김지형,차주완 한국환경과학회 2002 한국환경과학회지 Vol.11 No.12

This paper aims to find the characteristics of concentration distribution of coastal urban air pollutants. For this purpose, It was used the daily meteorological data and hourly concentration data O_3 and NO_2 in Busan metropolitan city from 1994 to 1996. It was investigated the annual and monthly distribution of ozone and nitrogen dioxide concentration at each site in Busan, and also investigated the characteristics of concentration change of air pollutants with time under the sea breeze. As a results, the concentration of nitrogen dioxide and ozone tend to be increased every year and nitrogen dioxide concentration is higher than ozone concentration at all site in Busan. The concentration of ozone is high in summer season and low in winter season, but the concentration of nitrogen dioxide have a reversed trend. The monthly peak concentration of ozone occurred in April and September, while the monthly minimum concentration of nitrogen dioxide occurred in August. Their trend were indentified by sites near the coastline than sites stands apart from the coastline. The sea breeze occurred annual mean 81 days in Busan from 1994 to 1996. The main wind direction of sea breeze was classified into southeasterly. In case of southwesterly, It was pronounced the south wind and southwest wind. In case of southeasterly, the occurrence frequency of east wind was high. Especially, the concentration of urban air pollutants, such as ozone and nitrogen dioxide, were high on time which the sea breeze flow, and the areas that ozone concentration was high moved from outside part to central part of city with time. In costal urban such as Busan, the wind direction of sea breeze is influenced the influenced the change of ozone and nitrogen dioxide concentration on time which the sea breeze flow at each site and air pollutants concentration of sites on the pathway of sea breeze.

배양된 해마 조직 절편에서 Beta-Amyloid Peptide의 신경 독성 효과

박정선,조미선,정연주,이경은 이화여자대학교 의과대학 2003 EMJ (Ewha medical journal) Vol.26 No.2

Alzheimer's disease(AD) is primarily characterized by neurofibrillary tangles, senile plaques, and neurodegeneration. The major component of senile plaques is the beta-amyloid peptide(A B), Which is considered to have a causal role in AD. However, the biological activities of AB in AD has not been clearly defined. In this study we have investigated the effects of AB 25-35 fragment to neurons using organotypic hippocampal slice culture system which maintained intact hippocampal synaptic circuit and anatomy. Hippocampal slice culture is prepared from rat pos-tnatal 10-old days and after 14 days culture, slices were treated with 10uM AB 25-35 fragment. Neuronal death was measured with propidium iodide(PI) uptake and NeuN, neuronal marker, staining. After treatment of AB 25-35 fragment for 3days or 7days on hippocampal slice culture, we observed the increased PI uptake and the decreased number of NeuN-stained neuron in CA1 region of hippocampal pyramidal layer or dentate gyrus. These results suggested that AB 25-35 fragment exerts the neurotoxicity in hippocampal slice culture.