인삼첨가 Long Life면의 조직감과 관능적 특성

정재홍,심창주,신영,권효진,이규희,오만진 한국식품영양학회 1999 韓國食品營養學會誌 Vol.12 No.5

인삼이 밀가루의 아밀로그래프에 의한 호화 성질과 파리노그래프에 의한 반죽 성질에 미치는 영향을 조사하였다. 인삼첨가 LL면의 조직감과 관능적 특성에 미치는 영향을 분석하기 위하여 밀가루에 대하여 인삼을 5.0∼10% 첨가하여 LL면을 제조한 뒤 면의 조직 특성 분석 및 조리시험을 하고 관능검사를 하였다. 인삼을 첨가하면 아밀로그래프의 호화개시 온도를 3.1℃ 지연시켰으며, 최고 점도를 40B.U 감소시켰다. 파리노그래프의 흡수율은 1.2% 증가하였으며, 반죽의 안정도는 인삼의 첨가로 2.0분 길게 측정되었다. Hunter L값은 인삼 첨가량이 증가할수록 낮아졌으며, Hunter a, b값은 반대로 측정되었다. 인삼첨가 LL면의 층밀림 압출력은 첨가량의 증가에 따라 높아져 10.0%의 첨가는 대조구 18.65(㎏f)보다 2.3(㎏f) 높은 23.95(㎏f)로 측정되었으며, 경도도 대조구보다 5.4(㎏f) 높게 측정되었다. 인삼첨가 LL면의 무게 증가는 인삼의 첨가량이 증가할 때 다소 높게 나타났으나. 부피 증가는 오히려 높게 나타나 조직이 다소 치밀한 것으로 나타났다 용출량은 인삼량의 증가에 따라 약간 감소하였다. 미생물은 인삼의 첨가량이 증가할수록 줄어들어서 장기 저장이 가능하였다. 관능 검사 결과는 인삼첨가 LL면이 4.2 및 4.0으로 비교적 좋은 점수를 얻었다. 이같이 LL면 제조시 인삼 첨가량은 7.0%의 수준이 효과가 컸다. The influence of ginseng on the paste or gelatinization properties by amylograph and mixing properties by farinograph of wheat flour, and on quality properties, color, cooking quality, textural and sensory properties, and reducing microbial population of LL (Long Life) noodles was studied. The contents of ginseng used were from 5% to 10% based on flour weight. The viscosity property of wheat flour with ginseng was increased the initial pasting temperature but the amylograph peak viscosity were decreased in vice versa. The farinograph absorption, stability and breakdown were increased by ginseng. The whiteness of LL noodles manufactured with ginseng was lower than that of control. The shear extrusion force and hardness of LL noodles manufactured with ginseng were shown much higher value than those of control. At cooking quality examination of LL noodles manufactured with ginseng, weight of cooked LL noodles was decreased but volume was appeared in vice versa. Extraction amounts of LL noodles manufactured with ginseng during cooking were much smaller than those of control. Total counts of microorganism of LL noodles manufactured with ginseng were decreased during storage at 30℃. Sensory properties of cooked LL noodles which was manufactured with ginseng showed quite acceptable. Based on the cooking and sensory evaluation test, addition of 7.0% ginseng to wheat flour may be suitable for processing LL noodles.

북한이탈주민의 외상 후 스트레스 장애와 자연살해세포 활성

김병창,정성훈,임효덕,최종혁,전정희,전양환 大韓神經精神醫學會 2008 신경정신의학 Vol.47 No.5

Objectives : The objective of this study was to evaluate an association between posttraumatic stress disorder (PTSD) and altered immunity m North Korean defectors who were more likely to develop medical conditions and other stress-related psychiatric disorders. Methods : Twenty-four North Korean defectors with PTSD and twenty-two controls without PTSD were recruited from the resettlement and training center for North Korean defectors in South Korea. Peripheral T lymphocyte subsets, natural killer(NK) cells, NK cell activity and plasma hormones (ACTH and cortisol) were obtained from all subjects. We also applied the Hamilton Rating Scale for Depression (HAM-D) and Hamilton Rating Scale for Anxiety (HAM-A) for all subjects. Results : We found NK cell activity was relatively lower than number of NK cells in North Korean defectors with PTSD. Subjects with PTSD had higher HAM-D and HAM-A scores than controls. However, there were no statistical differences in ACTH. cortisol, CD3, CD4, CD8, CD4/CD8 ratio, CD19 and CD56 between the two groups. Conclusion : These findings suggest North Korean defectors with PTSD show higher levels of depression, anxiety and impaired or weak immune function in NK cells.

비소세포폐암에서 Microsatellite Instability

전효성,김정란,손지웅,박선하,박태인,김창호,김인산,정태훈,박재용 경북대학교 병원 2001 경북대학교병원의학연구소논문집 Vol.5 No.1

연구베경:MMR 유전자의 불활성화에 의해 야기되는 뮤전적 불안정성은 발암기전의 한 부류로 인정되고 있다.저자들은 비소세포폐암의 발암과정에서의 MSI의 역할을 규명하기 위해 비소세포폐암에서 MSI의 빈도 및 MSI 유무에 따른 임상상의 차이를 조사하였다. 대상 및 방법:근치적 절제술을 받은 비소세포폐암 20예를 대상으로 하였다.동결된 폐암조직과 환자의 림프구에서 DNA를 추출한 후 3P와 9p의 15개의 marker들을 대상으로 PCR을 시행하고 7% polyacrylamide gel에서 전기영동한 후 silver 염색을 시행하였다. 암조직과 림프구 DNA의 PCR product의 band를 비교하여 MSI와 LOH를 판정하였다. 결과:1)대상환자들은 남자 19예,여자 1예였으며 모두 흡연자였고 평균 흠연력은 47 갑년이었다. 폐암의 조직형은 편평상피암 15예, 선암 4예,대세포암 1예였고, 술 후 병리학적 병기는 Ⅰ기 6예,Ⅱ기 5예, ⅢA기 7예,ⅢB기 2예였다. 2)20예 가운데 8예(40%)에서 MSI가 관찰되었으며 3예는 한 개의 marker에서,5예는 2개 이상의 marker에서 MSI가 관찰되었다. 3)LOH는 10예(50%)에서 있었으며,LOH유무에 따른 병가 및 흡연력의 차이가 없었다. 4)분석한 marker의 10% 이상에서 MSI가 관찰된 MSI-L종양은 5예였으며, 대부분의 marker에서 MSI양성인 MSI-H종양은 없었다.MSS종양과 MSI-L종양은 흡연력, 병기, 폐암 조직형 및 LOH 빈도의 유의한 차이가 없었다. 결론:비소세포폐암에서 MSI는 비교적 흔히 관찰되지만 MMR 유전자의 불활성화에 의한 MMP pathway 는 비소세포폐암의 주요 발생기전은 아닐 것으로 생각된다.향 후 비소세포폐암의 발암과정에 있어서 MMP pathway의 역할을 규명하기 위해서는 보다 많은 예를 대상으로 한 연구가 필요할 것으로 생각되며, MSI 발생기전에 관한 추가적인 연구가 필요할 것으로 생각된다. Purpose: Microsatellite instability(MSI) is frequently used as an indicator of microsatellite mutator phenotype (MMP) tumors.MSI has been observed in a percentage of non-small cell lung cancer(NSCLC).However, its role in tumorigenesis of NSCLC remains unknown.The frequency and partten of MSI in NSCLC were evaluted and clinical parameters of MSI-positive tumors with those of MSS(microsatellite stable) tumors were compared. Materials and Methods: Twenty surgically resected NSCLCs were analyzed for 15 microsatellite markers located at chromosomes 3p and 9p.The peripheral blood lymphocytes of patients were used as the source of the normal DNA. Results:1) of 20 cases, 8(40%) demonstrated MSI. 2) Instability was observed more frequently in tri- and tetra-nucleotide repeats that in dinucleotide repeats. In all cases, instability appeared as a shift of individual allelic bands. 3) LOH was observed in 10(50%) of 20 tumors analyzed. 4) Of 20 cases, MSI-H tumor(showing MSI in the majority of markers) was absent. There were 5 MSI-L tumors( showing MSI in a greater than 10% of markers). 5) No significant difference was observed between MSI-L tumors and MSI negative tumors in terms of clinicopathologic features such as pack-year history of smoking, histologic subtype, and (delete) stage of disease.There was also no significant difference in the incidence of LOH in relation to the status of MSI. Conclusion: These data strongly suggest that MSI plays different roles in lung and colon cancer.MMP pathway appears to be far less important in the tumorigenesis of NSCLC, caused mainly by cigarette smoke, with little familial tendency.(tuberculosis and Respiratory Diseases 2000,48:24-32)

대장암 주변에서 관찰되는 백색반점의 병리조직학적 특성과 임상적 의의

김효종,조진만,장린,동석호,김윤화,장영운,박일랑,김병호,이정일 대한소화기내시경학회 1999 Clinical Endoscopy Vol.19 No.4

Background/Aims: To investigate the difference between colorectal adenocarcinomas with white spots (foamy cells) and those without white spots, clinically and histopathologically, were examined 37 cases of colorectal adenocarcinomas were classified in this study. Methods: Two groups: those with white spots and those without. In each case, evidence of lymph node and liver metastasis was sought. Immunoreactive staining for macrophage was performed. Results: Increased incidence of exophytic tumors was found (73%, p=0.028) in the group with white spots. The incidences of lymph node metastasis were 33% vs. 45% between the group with white spots and the group of without, but the difference was not statistically significant. Conclusions: These results suggested that foamy cells might have a beneficial role in colorectal cancer, although they were not statistically significant. Therefore, further prospective study be warranted.

당귀의 품질평가법에 관한 연구

조창희,김수정,이남윤,우영아,김효진 동덕여자대학교 종합약학연구소 2001 동덕약학연구지 Vol.5 No.-

Angelicae gigantis radix is the root of the perennial plant, which belongs to family Umbelliferae. However, this herbal drug has quite different chemical components according to its different geographical origin though other herbal drugs (i.e. Leonuri Herba, Xanthii Fructus and so on) show similar major constituents. In other word, the root of Angelica gigas containing the coumarin compounds is commonly used in Korea as Angelicae gigantis radix, while Angelica sinensis and Angelica acutiloba including phthalide compounds are used in China and Japan, respectively. In this paper, Near-Infrared spectroscopy was developed to determine Genus name of Angelica spp., especially Angelica gigas and Angelica sinensis which are generally misused in the herbal market. Also X-ray fluorescence spectrometry was evaluated to analyze the differences between Angelica gigas and Angelica sinensis with their specific properties.

디지털 카메라를 이용한 2D 인체계측법 연구

손희정,김효숙,최창석,손희순,김창우 服飾文化學會 2003 服飾文化硏究 Vol.11 No.1

This study suggests the new 2D anthropometric method using digital camera. It is used MK2001 program that can convert 2D measurements to 3D measurements. To improve that it is measured 100 college student with direct and indirect anthropometric method. The measurements were processed by the SPSS ver10 Statistical Package. The average, standard deviation, and t-test were calculated for each category. Most measurements by 2D measurements are higher than direct measurements but degree. The difference between direct and indirect measurements is less than 2cm. In the results of t-test, height measurements including other 16 measurements which is easy to measure have no meaningful difference within 1cm. The depth measurements are most high difference. The result of each measurement proves that MK2001 program (2D anthropometry method using digital camera) is available for measuring the human body.

Methylphenidate 투약에 따른 주의력결핍 과잉운동장애 아동의 정량화 뇌파의 변화

이병대,정성훈,이만기,장수민,백지령,임효덕 대한생물치료정신의학회 2002 생물치료정신의학 Vol.8 No.1

Lipids Induce Release of Tumor-Promoting Exosomes from Hepatocellular Carcinoma Cells

( Eun Ju Cho ),( Hyo Yeong Lee ),( Joon Yeul Nam ),( Young Chang ),( Hyeki Cho ),( Young Youn Cho ),( Jeong-hoon Lee ),( Su Jong Yu ),( Yoon Jun Kim ),( Jung-hwan Yoon ) 대한간학회 2017 춘·추계 학술대회 (KASL) Vol.2017 No.1

Aims: Hypoxia enhances lipid droplets accumulation by HIF-dependent mechanism, leading to intra-tumoral fatty metamorphosis which is one of the characteristics of early-stage hepatocellular carcinoma (HCC). We investigated whether lipids and lipids-induced exosomes released from HCC cells can induce a tumor-promoting phenotype. Methods: Human HCC cell lines (Huh-7, SNU-761, and SNU-3058) were incubated with oleic acid (OA) or control vehicle. The released exosomes were isolated, quantified, and applied to HCC cells. Results: Incubation of Huh-7 and SNU-761 cells with OA increased proliferation and migration of cells in a fatty-acid binding protein 3 (FABP-3) dependent manner, whereas SNU-3058 cells did not respond to OA. Furthermore, OA upregulated FABP-3 mRNA expression in Huh-7 and SNU-761 cells, whereas its expression in SNU-3058 cells did not change. OA enhanced release of exosomes from Huh-7 and SNU-761 cells, and exosomes collected form these cells upregulated proliferation and invasion of cells. However, this was not observed in SNU-3058 cells. Exosomes released from OA-treated cells were reduced by the inhibition of rho-associated, coiled-coil-containing protein kinase 1 (ROCK1) or FABP-3. Conclusions: These findings suggest that lipids induce release of exo-somes containing unique cargoes from HCC cells in a FABP-3 dependent manner, which may induce the progression of HCC.

Nitrogen-incorporated (Ba, Sr)$TiO_3$ thin films fabricated by r.f.- magnetron sputtering

Lim, Won-Taeg,Jeong, Yong-Kuk,Lee, Chang-Hyo The Korean Vacuum Society 2000 Journal of Korean Vacuum Science & Technology Vol.4 No.4

In this study, two kinds of barium strontium titanate (BST) samples were prepared. One is a conventional BST film that is sputtered in a mixture of argon and oxygen. The other is a nitrogen-incorporated BST film that is sputtered in a mixture of oxygen and intentionally added nitrogen instead of argon gas. The structural properties of both of the BST films had not changed significantly with the species of sputtering gas. However, the leakage current of BST films sputtered at ($N_2$+O$_2$) atmosphere was lower than those sputtered at (Ar +O$_2$) atmosphere: 1.9$\times$10$^{-8}$ A/cm$^2$ at 2V for the films prepared at (Ar +O$_2$) atmosphere and 8.6$\times$10$^{-9}$ A/cm$^2$ for the films at ($N_2$+O$_2$) atmosphere. From an XPS analysis, it has been found that nitrogen atoms are incorporated in BST films with a concentration of 1.92 at% and form a certain oxynitride phase. It is proposed that nitrogen atoms are able to fill the oxygen vacancies of BST films during sputtering process, and then the leakage current reduces due to a decrease in the vacancies. The BST films sputtered at ($N_2$+O$_2$) atmosphere have superior electrical properties to the films sputtered at (Ar +O$_2$), without any significant structural changes.

Inhibitory Effect of Clavicepitaceae on Serotonin Release out of Human Platelets and Human Platelet Aggregation

박화진,--,--,--,--,--,-- THE KOREAN SOCIETY FOR BIOMEDICAL LABORATORY SCIEN 2004 Journal of biomedical laboratory sciences Vol.10 No.1

We have investigated the effects of hypha-water extracts (HWE), fruit body-water extracts (FWE) and cordycepin from Cordyceps militaris on serotonin release out of human platelets and human platelet aggregation. HWE and FWE inhibited the release of [^(3)H]-serotonin from human platelet stimulated by thrombin (2 U/ml) or collagen (20 ㎍/ml) in a dose-dependent manner. Furthermore, cordycepin, a major component of Cordyceps militaris, inhibited the human platelet aggregation induced by collagen (10 ㎍/ml) in a dose-dependent manner. These results suggest that cordycepin containing in HWE and FWE may inhibit the serotonin release by suppressing the collagen-induced human platelet aggregation. Accordingly, our data demonstrate that HWE and FWE containing much cordycepin might have antithrombotic and antimigrainous functions.