LC-MS/MS를 이용한 넙치(Paralichthys olivaceus)시료의 톨트라주릴 및 톨트라주릴 설폰 분석

홍도희,김아현,이가정,윤민철,손광태,김명석,김나영,정승희,조미라 한국수산과학회 2019 한국수산과학회지 Vol.52 No.5

Several studies investigating the prevention and treatment of external parasites in farmed olive flounder Paralichthys olivaceus have found that the anticoccidial agent toltrazuril sulfone is an effective antiparasitic. Prior to undertaking a full-scale study, we developed analytical methods to detect the levels of toltrazuril and toltrazuril sulfone in farmed flounder samples using liquid chromatography-electrospray ionization tandem mass spectrometry (LC-MS/MS). This analysis showed that LC-MS/MS changed the mobile phase and collision energy of toltrazuril and toltrazuril sulfone. This was validated using established conditions. Sample pre-treatment for this process involved extraction with dichloromethane and purification by liquid–liquid extraction in formic acid, acetonitrile, and h-hexane, followed by determination of all compounds by LC-MS/MS. Separation was achieved within 10 min by gradient elution using a Capcell Pak C18 (3.0 μm, 100×2.0 mm) analytical column (Shiseido UG 120V) with a mixture of 0.1% (v/v) formic acid and acetonitrile. Multiple reaction monitoring was used for selective detection of toltrazuril and toltrazuril sulfone. This method yields satisfactory results for linearity, precision, and limits of quantification. Therefore, the method established in our study will serve as a basis for further research on parasite control by toltrazuril and toltrazuril sulfone.

제30차 학술대회 : 65. 담도계 질환의 임상적 고찰

홍도희,전홍준,김종훈,황정열,박주섭 () 대한외과학회 1978 대한외과학회 학술대회자료집 Vol.- No.-

녹차를 첨가한 감자떡의 저장성 평가

홍도희,류아라,김아현,문호진,이경실,이정운,김정목 한국식품영양과학회 2015 한국식품영양과학회 학술대회발표집 Vol.2015 No.8

LC-MS/MS를 이용한 수산물 중 Toltrazuril 분석법 개선 연구

홍도희,강은혜,이수광,윤민철,유홍식,손광태,조미라,김명석,김나영,정승희 한국수산과학회 양식분과 2019 한국수산과학회 양식분과 학술대회 Vol.2019 No.5

우리나라 연안해역 생산 패류 중 다환방향족탄화수소 분석

홍도희 ( Do Hee Hong ),강은혜 ( Eun Hye Kang ),윤민철 ( Minchul Yoon ),조미라 ( Mi Ra Jo ),손광태 ( Kwang Tae Son ),이가정 ( Ka Jeong Lee ) 한국수산과학회 2021 한국수산과학회지 Vol.54 No.4

Polycyclic Aromatic Hydrocarbons (PAHs) were analyzed and safety evaluation was carried out in Korean coastal area. The target congeners were benzo[a]pyrene, benzo[a]anthracene, benzo[b]fluoranthene, and chrysene. The method of analysis was significant. The highest PAHs 4 congener concentrations (12.2 μg/kg) was found in the mussel of Gangwon area. Comparison among the average concentration of PAHs for each congener showed that, benzo[a] anthracene was found in mussel (0.276 μg/kg), oyster (0.463 μg/kg), ark shell clam (1.92 μg/kg), and chrysene was found in mussel (0.848 μg/kg), oyster (1.36 μg/kg), scallop (0.489 μg/kg), sea squirt (3.07 μg/kg), and ark shell clam (0.449 μg/kg). In addition, benzo[b]fluoranthene was found in mussel (0.253 μg/kg), scallop (0.244 μg/kg), and sea squirt (1.64 μg/kg). The most hazardous benzo[a]pyrene was found in mussel (0.147 μg/kg), and scallop (0.244 μg/ kg), it was not detected in the other shellfishes. However, all the PAHs levels recorded in this study did not exceed international MRLs. There was no significant difference among the PAHs concentration in shellfish based on sampling area or species. Body exposure was calculated based on PAHs concentration and intake rate. The results of this study indicated that PAHs in shellfish were within acceptable safe levels.

녹차를 첨가한 감자떡의 저장 중 품질변화

홍도희(Do Hee Hong),김아현(Ah-Hyun Kim),조종락(Jong-Lak Cho),김정목(Jeong-Mok Kim) 한국차학회 2022 한국차학회지 Vol.28 No.1

본 연구는 전통향토음식인 감자떡에 녹차분말, 녹차유 및 녹차농축액을 첨가하여 개발한 제품에 이들이 미치는 영향을 살펴보았다. 녹차 감자떡의 저장성을 확인하기 위해 저장시간에 따른 총균수 변화 뿐만 아니라 조직감, 관능평가도 실시하였다. 감자떡의 반죽에는 감자전분과 찹쌀가루 중량 기준으로 녹차가루 0.5%, 녹차유 3%, 또는 녹차가루 0.5%와 녹차유 3% 혼합한 것을 넣고 저장 시간에 따른 총균수를 측정한 결과, 녹차유 3%만 첨가한시료가 가장 낮은 총균수를 나타내었으나 각 시료간의 유의적인 차이는 없었다. 앙금에도 녹차분말 1~3% 또는 녹차농축액 1~3% 첨가하여 총균수를 측정한 결과, 녹차농축액의 양이 많을수록 총균수는 감소하였다. 반죽에 0.5% 녹차분말과 3% 녹차유를 첨가하고 앙금에 3% 녹차농축물을 첨가하여 제조한 녹차 감자떡이 가장 높은 저장성을보여주었고 미생물학적 측면에서도 안전성을 보였다. 관능 평가에서 전체 기호도는 앙금에 3% 녹차농축액을 첨가한 녹차 감자떡 시료가 가장 높았다. 연구 결과를 통해서 감자떡 반죽에 녹차유의 첨가는 떡의 노화를 억제하고 식감을 개선시켰으며, 팥앙금에 녹차농축액을 첨가함으로써 저장성을 높이는데 효과를 보였다. The purpose of this study was to investigate the quality of potato rice cakes containing green tea powder (GTP), green tea oil (GTO), and green tea essence (GTE). To confirm the storability of green tea potato rice cakes (GPC), sensory evaluations and assessments of the texture as well as the changes in the total microbial numbers according to the storage time were performed. GTP 0.5%, GTO 3%, or a mixture of 0.5% GTP and 3% GTO based on the weight of potato starch and glutinous rice flour were added to the GPC dough, and the total microbial numbers in the dough according to the storage time was measured. The sample to which only 3% GTO was added showed the lowest total microbial numbers, but there was no significant difference between the samples. The total microbial numbers were measured after adding 1~3% of GTP or 1~3% of GTE to the sediment, and the results showed that the higher the GTE content, lower the total microbial numbers. GPC prepared by adding 0.5% GTP and 3% GTO to the dough and addition of 3% GTE to the sediment showed the highest storage stability and safety in terms of microbiological characteristics. In the sensory evaluation, the overall taste was highest in the GPC sample, with the addition of 3% GTE to the sediment. In conclusion, GTO addition to the dough slowed down the aging of the GPC, and the addition of GTE to the red bean sediment, which had poor storage properties, enhanced the storage properties and improved the texture.

소화성궤양의 외과적 합병증에 대한 임상적 고찰

홍도희(Do Hee Hong),박주섭(Ju Sub Park) 대한외과학회 1981 Annals of Surgical Treatment and Research Vol.23 No.8

Bile Duct Adenoma : a case report

황호원,홍도희,서재홍 최신의학사 1989 最新醫學 Vol.32 No.1

다소비 해조류 섭취에 의한 유해중금속의 식이노출평가

강은혜,홍도희,박지인,이가정,조미라,유홍식,하광수,손광태,윤민철 한국수산과학회 2022 한국수산과학회지 Vol.55 No.6

In the present study, exposure to heavy metals by consumption of edible seaweeds (green laver, laver, hijiki, sea tangle, and sea mustard) was assessed based on their concentrations of lead (Pb), cadmium (Cd), arsenic (As), and mercury (Hg). The mean of heavy metal concentrations were 0.006–0.023 mg/kg for Pb, 0.037–0.156 mg/kg for Cd, 1.117–15.928 mg/kg for As, and 0.008–0.021 mg/kg for Hg. In multivariate analysis, the correlations were high between Pb levels in sea mustard, Cb levels in laver, and As and Hg level in Hijiki. However, the estimated daily intake and target hazard quotient (THQ) of the heavy metals in edible seaweeds were below their approved limits suggesting no health risks associated with seaweed consumption by Koreans.

白鼠 摘出心臟에서 冠狀 血流에 미치는 Verapamil, Nicardipine 및 Diltiazem 의 影響

崔峰奎,洪度熹 圓光大學校 醫科學硏究所 1992 圓光醫科學 Vol.8 No.1-2

It was the aim of the present study to gain more insight into the role of calcium from both extracellular and intracellular sources in the regulation of coronary arterial tone. The effects of verapamil, nifedipine and diltiazem on the cardiac function, especially in coronary flow rate were compared in the isolated working rat heart. Also the influences of these drugs on the effects of phenylephrine (PE), clonidine and methacholine (MCh) which were well known as coronary spasmogens, were investigated. When introduced into the left atrium, verapamil, a Ca^2+-channel blocker in doses of 10^-7-10^-5M decreased heart rate and coronary flow rate. The moderate doses of other types of Ca^2+-antagonists, nicardipine (10^-7-10^-6M) and diltiazem (10^-7-10^-5M), decreased the coronary arterial resistance and increased the coronary flow with dose-dependent manner, with diltiazem having more potent than nicardipine. The adrenergic agents, phenylephrine (10^-4M) and clonidine (10^-4M), as well as the parasympathomimetics methacholine (10^-5M) increased coronary arterial resistance and decreased coronary flow rate. and the effects produced by phenylephrine and clonidine were blocked by Ca^++-entry blockers. However, the effect of methacholine was blocked by nicardipine but not by verapamil and diltiazem. Bay K 8644, a calcium-channel agonist, increased coronary arterial resistance and decreased coronary flow rate, and the effects of silmultaneous administration of Bay K 8644 (10^-5M) and phenylephrine (10^-4M) were much greater than those of single agents. Bay K 8644 inhibited the effect of nicardipine (10^-7M) but not that of diltiazem (10^-7M). From these results, it is suggested that the decreases of the coronary flow by phenylephrine and clonidine are due to coronary vasoconstriction which is caused by extracellular Ca^++ influx. However, the methacholine-induced contriction is not related to the Ca^2+ influx. It is thus concluded that the constriction of coronary vessel caused by activation of symphathetic nervous system is blocked by Ca^++ entry blockers, whereas the effect caused by activation of parasympathetic nervous system is not susceptible to Ca^++ entry blockade, but blocked by nicardipine.