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위선종, 위선암에서 Bcl-2, Bcl-xL, Bax, p53 단백의 면역조직화학 발현
이동수 ( Dong Soo Lee ),강상범 ( Sang Bum Kang ),백종태 ( Jong Tae Baek ),남순우 ( Soon Woo Nam ),이강문 ( Kang Moon Lee ),안병민 ( Byung Min Ahn ),이은희 ( Eun Hee Lee ),한석원 ( Sok Won Han ),정인식 ( In Sik Chung ) 대한소화기학회 2005 대한소화기학회지 Vol.45 No.6
Background/Aims: The aim of this study was to investigate the immunohistochemical expression of bcl-2, bcl-xL, bax, and p53 proteins according to the pathological parameters such as grade of dysplasia, histological type, depth of invasion, lymph node meta
$2{\beta}$, $3{\alpha}$, 23-trihydroxyrus-12-ene-28-oic acid처리에 의한 인간 간암세포주 HepG2의 apoptosis 유도
유기현,이종민,황보전,송명종,양혜정,백남인,김성훈,김대근,권병목,박미현,정인식,Yoo, Ki-Hyun,Lee, Jong-Min,HwangBo, Jeon,Song, Myoung-Chong,Yang, Hye-Joung,Baek, Nam-In,Kim, Soung-Hoon,Kim, Dae-Keun,Kwon, Byoung-Mok,Park, Mi-Hyun,Chung, 한국응용생명화학회 2006 Applied Biological Chemistry (Appl Biol Chem) Vol.49 No.4
Triterpenoid를 포함하고 있는 $2{\beta},\;3{\alpha}$, 23-trihydroxyrus-12-ene-28-oic acid를 애기마름으로부터 분리하였다. 이것은 pentacyclic triterpenes의 공통 구조를 가지며 amyrin ursolic acid 그룹에 속해 있다. 본 연구에서는 이 화합물의 독성 영향을 인간 간암 세포주인 HepG2에서 조사하였다. $2{\beta},\;3{\alpha}$, 23-trihydroxyrus-12-ene-28-oic acid는 처리한 양에 비례하여 HepG2 세포주에서 독성을 보였다. 그리고 Confocal microscopy 결과는 $2{\beta},\;3{\alpha}$, 23-trihydroxyrus-12-ene-28-oic acid를 HepG2 세포에 처리한 시간에 비례하여 녹색 형광의 증가를 보여주었다. $2{\beta},\;3{\alpha}$, 23-trihydroxyrus-12-ene-28-oic acid는 또한 HepG2 세포의 sub-G1 cell population 뿐만 아니라 DNA 분절(fragmentation) 현상의 증가를 보여 주었다. 이러한 결과는 $22{\beta},\;3{\alpha}$, 23-trihydroxyrus-12-ene-28-oic acid가 HepG2 세포에서 apoptosis를 통한 세포 사멸 유도를 의미한다. [ $2{\beta},\;3{\alpha}$ ], 23-trihydroxyrus-12-ene-28-oic acid was isolated from Trapa pseudoincisa S. et Z. It has a common structure of pentacyclic triterpenes and belongs to the amyrin ursolic acid group. The cytotoxic effect of this compound was investigated in human hepatoma cell line HepG2. $2{\beta},\;3{\alpha}$, 23-trihydroxyrus-12-ene-28-oic acid showed dose-dependent cytotoxicity in HepG2 cells. Confocal microscopy data showed that green fluorescence was increased in $2{\beta},\;3{\alpha}$, 23-trihydroxyrus-12-ene-28-oic acid treated-HepG2 cells in a time-dependent manner. $2{\beta},\;3{\alpha}$, 23-trihydroxyrus-12-ene-28-oic acid also increased the sub-G1 cell population of HepG2 cells as well as ladder-like DNA fragmentation. Taken together, our results indicate that $2{\beta},\;3{\alpha}$, 23-trihydroxyrus-12-ene-28-oic acid induced apoptosis in HepG2 cells.
Kyung Hwa Chang,유기현,황보전,Yeon Ju Seok,Jong Min Lee,김경일,이윤형,양재명,백남인,정인식 한국응용생명화학회 2009 Journal of Applied Biological Chemistry (J. Appl. Vol.52 No.1
Expression of the human cyclooxygenase 1 (COX-1) in Trichoplusia ni BTI Tn 5B1-4 cells transformed with cDNAs encoding β1,4-galactosyltransferase (GalT) and Galβ1,4-GlcNAc α2,6-sialyltransferase (ST6Gal) was examined. Southern blot analysis indicated that the glycosyltransferase genes were integrated into the Tn 5B1-4 cell genome. A lectin blot analysis also indicated that the recombinant COX-1s from the Tn 5B1-4/COX-1/GalT-ST cells contained the glycan residues of β1,4-linked galactose and α2,6-linked sialic acid. The specific peroxidase activity of the recombinant sialylated COX-1 from Tn 5B1-4/COX-1/GalT-ST cells was 23,930 units/mg, indicating an increase of approximately 17% compared with the non-sialylated control (20,500 units/mg) from the Tn 5B1-4/COX-1 cells.
궤양성 대장염의 Antineutrophil Cytoplasmic Antibody ( ANCA ) 발현율
한상원(Sang Won Hang),오수혁(Soo Hyuk Oh),정현(Hyun Jung),양영상(Young Sang Yang),채현석(Hyun Seok Chae),이봉수(Bong Soo Lee),서정민(Jung Min Suh),김재광(Jae Kwang Kim),백남종(Nam Jong Baeg),최규용(Kyu Yong Choi),정인식(In Sik Jung) 대한소화기학회 1994 대한소화기학회지 Vol.26 No.3
N/A Antineutrophil cytoplasmic antibodies (ANCA) are autoantibodies against various antigens present in the cytoplasmic primary granules of neutrophils detected by standard indirect immunofluorescent technique fixing the neutrophil with alcohol. There are two types of ANCA characterized by immunoreactivity, perinuclear type AACA(P-ANCA) and cytoplasmic type ANCA(C-ANCA). ANCA was demonstrated in the sera of patients with inflammatory bowel disease. We have evaluated the reactivity against human neutrophils of sera from 44 patients with ulcerative colitis, 4 Crohns disease, 3 tuberculous colitis, 2 Behqets disease, 10 simple co- litis and 25 healthy controls. 26/44 (59.1%) sera from patients with ulcerative colitis con- tained ANCA compared with 2/4 (50.0%) from Crohns disease, 1/2 (50.0%) from Behqets disease and 2/25 (8%) from control sera. All ten from patients with simple colitis and all three sera from tuberculous colitis were negative. The presence or titer of ANCA was not cor- related with activity of treatment of ulcerative colitis. The predominant pattern of ANCA was cytoplasmic (30/31) and only one serum gave a perinucleatr pattern. In conclusion, ANCA occurred more commonly in ulcerative colitis than tuberculous colitis or simple colitis.(Korean J Gastroenterol 1994;26: 451 457)
이종민,마상동,한태룡,김공환,임상빈,정인식 경희대학교 생명자원과학연구원 1997 遺傳工學論文集 Vol.9 No.-
Supercritical fluids (SCF) are useful substances that are recently used in bioseparation process. Extraction of carthamin from safflower using supercritical carbon dioxide were examined at various conditions of temperature (35∼40℃), pressure (3000∼5000 psi) and CO₂ flow rate (900∼1200 m/hr). SCF was less effective than solvent methods in carthamin extraction from safflower. Concentration of carthamin was 50% more efficent at the use of alginate beads with 35 g cellulose per column loadings compared to the control (15g cellulose per column loadings).
Trichoplusia ni 세포의 apoptosis 메커니즘 규명을 위한 기초연구
이종민,정인식,양재명,이윤형 한국농화학회 2001 Applied Biological Chemistry (Appl Biol Chem) Vol.44 No.1
To elucidate the apoptosis mechanism of Trichoplusia ni cell, fundamental studies for apoptosis induction and suppression were performed. Hygromycin B, a known inducer of apoptosis, started the inhibition of T. ni cell growth at 200 ㎍/㎖ concentration. Furthermore, at 400 ㎍/㎖ concentration, DNA fragmentation was detected on day 2 of incubation. Although both dexamethasone and sodium butyrate inhibited T. ni cell growth, DNA fragmentation was not detected by both treatments. Also, when apoptosis induced T. ni cells with 200 ㎍/㎖ hygromycin B were treated with caspase inhibitor (Ac-DEVD-CHO), the apoptotsis was suppressed by 36%. In addition, N-acetylcysteine, another apoptosis repressor, also inhibited the apoptosis of T. ni cells. In order to express the anti-apoptosis gene (bcl-2), T. ni cells were transiently transformed with bcl-2 and its expression was confirmed by western blot analysis. These results showed the potential of developing new insect cell lines with suppressed apoptosis.
홍성현,박성길,이종민,한태룡,백영숙,정인식 경희대학교 생명자원과학연구원 1999 硏究論文集 Vol.20 No.-
홍화로부터 열에 의한 안정성을 검토하였다. 황색소의 분해반응은 산성, 중성, 염기성 조건에서 UV/Vis spectral measurement에 의해 조사되었다. 시간변화에 다른 흡광도를 측정해 본 결과 온도가 상승함에 따라 황색소의 분해되는 정도가 점차적으로 증가하는 것을 확인 할 수 있었다. 40℃에서 황색소의 분해 반감기는 pH3.0, pH7.0, 그리고 pH12.0에서 각각 112.1, 39.3 과 25.3 시간이었다. 이것은 황색소가 pH3.0에서 비교적 더 안정함을 말한다. pH3.0, 7.0 그리고 12.0에서 황색소의 activation energy는 각각 519, 15.0 그리고 12.3 kJ/mol이었다 We investigated thermal stability of yellow pigment from Carthamus tinctorious L. Decomposition reactions of yellow pigment were monitored at acidic, neutral and alkaline conditions by UV/Vis spectral measurement. Decomposition of yellow pigment increased as temperature increased Decomposition half lives of yellow pigment at 40℃ were 112.1 39.3, and 25.3 hrs at pH 3.0 , pH 7.0, and pH 12.0, respectively, indicating that yellow pigment is more stable at pH 3.0. The activation energies of yellow pigment at pH 3.0, pH 7.0, and pH 12.0 were 51.9, 15.0, and 12. 3 kJ/mol, respectively.
EGFP또는 EPO를 안정하게 발현하는 Drosophila melanogaster S2 세포의 현탁배양
이종민,정인식 경희대학교 산학협력기술연구원 2002 산학협력기술연구논문집 Vol.8 No.-
Recombinant plasmids harboring heterologous genes coding enhanced green fluorescent protein(EGFP) and erythropoietin(EPO) were transfected and expressed in Drosophila melanogaster S2 cells. Stably transformed cell populations expressing EGFP or monkey EPO were isolated after 4 weeks of selection with hygromycin B. The recombinant EGFP expressed in transformed S2 cells had a molecular weight(MW) of 27 kDa, and was found primarily in the intracellular fraction. The monkey EPO, which was expressed in stably transformed S2 cells, was secreted into the medium with a MW of 24-26 kDa. the two sizes of recombinant EPO may be due to heterogeneity of the glycosylation. In a spinner flask culture, the recombinant monkey EPO was secreted into the medium up to 15.6 mg/L at 9 days of incubation. This findings demonstrate the successful expression of EGFP and monkey EPO in polyclonal Drosophila S2 cells, the use of EGFP as a repoter to analyze gene expression, and potential of a Drosophila cell-expression system for recombinant protein production as an alternative to a baculovirus-insect cell expression system.