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이종민,마상동,한태룡,김공환,임상빈,정인식 경희대학교 생명자원과학연구원 1997 遺傳工學論文集 Vol.9 No.-
Supercritical fluids (SCF) are useful substances that are recently used in bioseparation process. Extraction of carthamin from safflower using supercritical carbon dioxide were examined at various conditions of temperature (35∼40℃), pressure (3000∼5000 psi) and CO₂ flow rate (900∼1200 m/hr). SCF was less effective than solvent methods in carthamin extraction from safflower. Concentration of carthamin was 50% more efficent at the use of alginate beads with 35 g cellulose per column loadings compared to the control (15g cellulose per column loadings).
Sung-Bae Kim,Myung-Hwan Kim,Sung-Koo Lee,Tae-Won Kim,Cheolwon Suh,Jeong-Sik Shin,Jung-Sun Park,Eun-Soon Kim,Gyungyub Gong,Jung-Shin Lee,Woo-Kun Kim,Sang-Hee Kim 대한암학회 2003 Cancer Research and Treatment Vol.35 No.6
Purpose: Mutations in the p53 gene are reported in 50~90% of gallbladder and bile duct cancer, and have beenimplicated in chemoresistance. We undertook this studyto determine whether the introduction of the wild type p53gene into GBCE (human gallbladder cancer cell line witha heterozygous p53 mutation) by an adenoviral vectorcould increase the sensitivity of the cell to 5-FU, a commonlyused drug in the treatment of gallbladder cancer.Materials and Methods: GBCE cells were transfectedwith either Ad/p53 or Ad/E1 in the presence of 5-FU. Geneexpression was confirmed by western blotting. Nude micewere injected subcutaneously with GBCE cells. Whentumors formed, intratumoral injection of Ad/p53 wasperformed. Reduction of tumor size was compared in twoweeks of Ad/p53 gene transfection.Results: Ad/53 transfection induced a dose-dependentinhibition of tumor growth. Tumor colony formation wasmore inhibited with p53 gene transfection than with mocktransfection in the presence of 5-FU. The reduction intumor size was more pronounced with p53 transfectionthan with mock infection.Conclusion: These treatment modalities could be utilizedin the treatment of p53 mutant human gallbladdercancers. (Cancer Res Treat. 2003;35:521-527)
Kim, TaeHee,Kwon, Ye Eun,Park, Sun Min,Kim, Min Seok,Jeong, Young Hwan,Park, Se Yeong,Bae, Young-Soo,Cheong, Eun Ju,He, Yi-Chang,Gong, Chun,Gao, Wei,Kim, Hee Kyu,Ham, Yeon Ho,Kim, Jin-Kyu,Choi, Sun Eu Institute of Forest Science 2022 Journal of Forest Science Vol.38 No.3
Genus of Rhododendron has been used in traditional medicine since ancient times and is known to be effective in immune function, inflammation, and cold symptoms. And the reason for this activity is the flavanonol type among flavonoids in the genus of Rhododendron. Among the flavanonol types, Taxifolin-3-O-arabinopyranoside was isolated from the root of native R. mucronulatum in Korea, and the structure was finally identified through HPLC, LC-MS/MS, <sup>1</sup>H-NMR, and <sup>13</sup>C-NMR. Taxifolin-3-O-arabinopyranoside is a compound mainly found in R. mucronulatum, a representative species of the genus of Rhododendron, and exhibits antioxidant, anti-inflammatory, and anti-atopic activities. In this study, Taxifolin-3-O-arabinopyranoside was chemotaxonomic significant in 5 species of the genus Rhododendron native to Korea (R. mucronulatum, R. mucronulatum var. ciliatum, R. schlippenbachii, R. yedoense var. Poukhanense, R. japonicum for. Flavum). Compared with the existing literature, Taxifolin-3-O-arabinopyranoside was identified for the first time in 4 species of Rhododendron except for the R. mucronulatum.
태권도장의 서비스회복 공정성이 수련생 만족, 전환비용 및 충성도에 미치는 영향
김선공(Kim, Sun-Gong),이정흔(Lee, Jeong-Heun),성종환(Sung, Chong-Hwan),김현미(Kim, Hyun-Mi),염정환(Yum, Jung-Hwan) 한국체육과학회 2012 한국체육과학회지 Vol.21 No.4
The purpose of this study was to examine the relationship among service recovery justice, trainees’ satisfaction, switching costs and loyalty of Taekwondo training centers. The subjects were parents of Taekwondo trainees from ten Taekwondo training centers, and 340 data were collected and 327 of them were chosen as for final data analysis. Data processing was done for frequency analysis using SPSSWIN Ver. 18.0 and confirmatory factor analysis and structural equation model analysis using AMOS 7.0. Based on the above study method and procedures, the result of the study are summarized as follows. First, service recovery justice had significant influence on trainees’ satisfaction. Second, trainees’ satisfaction had significant influence on switching costs and loyalty. Third, switching costs had significant influence on loyalty.
김환영(Hwan Yung Kim),이동호(Dong Ho Lee),김시철(Si Chul Kim),공병근(Byung Gun Gong) 한국철도학회 2011 한국철도학회 학술발표대회논문집 Vol.2011 No.10
Conventional railways are less competitive than other land transportation means in term of speed and thus users preference and transportation share for rail system are relatively lower than others. For example most of the conventional lines except the Seoul~Busan corridor run at an average speed of 70 km/h or less which imposes certain constraints on roles and functions as the trunk lines. In this regard the speed of the conventional lines should be improved up to 200 km/h to gain competitiveness promote balanced regional development and lead the era of low carbon green growth. As track system is one of the most important elements for the speed-up it is critical to come up with optimum technical solutions. Improvement of ballast track structure with efficient track installation can provide structural stability for higher speed and ensure operational safety with lower maintenance efforts. Thus this study focuses on consequences followed by the speed-up including increase of load imposed on the track and impacts on track components and provide solutions for track maintenance by analyzing impact on the track structure by speed. Also it compares ballast and concrete tracks under designing and construction and considers how to meet needs for passengers comfort and environmental requirements as a strategic approach.
Matti-Russe 술식과 Fisk-Fernandez 술식을 이용한 주상골 불유합 치료에 관한 비교 연구
이승환(Seung Hwan Rhee),정문상(Moon Sang Chung),이영호(Young Ho Lee),공현식(Hyun Sik Gong),김지영(Ji Yeong Kim),김민범(Min Boem Kim),오원석(Won Seok Oh),전숙하(Suk Ha Jeon),김정환(Jeong Hwan Kim),백구현(Goo Hyun Baek) 대한정형외과학회 2008 대한정형외과학회지 Vol.43 No.6
목적: 확정형 주상골 불유합에 대하여 적절한 치료를 시행하지 않는 경우 수근골 불안정 및 골관절염의 진행을 피할 수 없는 것으로 알려져 있다. 본 연구에서는 Matti-Russe 술식과 Fisk-Fernandez 술식을 이용한 골유합술의 임상적 및 방사선학적 결과에 대해 비교, 고찰하고자 한다. 대상 및 방법: 2001년 9월부터 2006년 8월까지 본 병원에서 시행한 주상골 골유합술 증례 중 인터뷰에 응한 21례를 대상으로 하였고 평균 추시기간은 31개월이었다. 임상적 평가로는 관절운동범위, 파악력, 환자만족도, 동통 수준을 설문조사하고, 방사선학적 평가로는 골유합, 무혈성괴사, 골관절염 및 DISI 여부를 평가하였다. 또한, 두 수술군 사이에 결과의 차이가 있는지를 비교하였다. 결과: 두 수술군 사이에 동통이나 만족도의 유의한 차이는 없었다, 관절운동범위, 파악력 모두 건측에 비하여 감소한 소견을 보였으나 역시 두 수술군 사이에는 유의한 차이가 없었다. 두 수술군의 골유합률 및 수술 전후 DISI 교정각 사이에도 유의한 차이는 없었고 술 전 관찰된 DISI는 술 후 모두 교정된 소견을 보였다. 골관절염의 경우 전체적으로 7예에서 관찰되었는데, 그 중 술 전에 없던 골관절염이 새롭게 나타난 경우가 1예 있었다. 결론: 두 수술군 사이에 임상적, 방사선학적 평가에 있어서 통계적으로 유의한 차이를 보이지 않으며, 두 술식 모두 골유합과 기능적 회복에 있어서 효과적인 방법이다. Purpose: Established scaphoid nonunion may lead to carpal instability and osteoarthritis. The purpose of this study is to compare the clinical and radiological results of the osteosynthesis between Matti-Russe group and Fisk-Fernandez group. Materials and Methods: Our retrospective study included 21 nonunion cases in 20 patients treated by Matti-Russe or Fisk-Fernandez technique from September 2001 to August 2006. The mean duration of follow up was 31 months. Based on the questionnaire which included clinical information and the radiologic evaluation, we compared the objective and subjective results between two surgical groups. Results: There were no significant differences between two groups in respect of the postoperative satisfaction and the pain. Both the range of motion and the grip power decreased compared to that of the unaffected side, but there were no significant differences between two groups. Also, there were no significant differences in the union rate and the rate of successful correction of DISI deformity. In all cases in which DISI deformity was detected preoperatively, the deformity was corrected postoperatively. There were 7 cases of osteoarthritis, but among them only one case appeared newly after the surgery. Conclusion: There were no significant differences between two surgical groups in clinical and radiological results. Both techniques were effective in achieving bony union and functional recovery.