화장품소재로서의 인삼복합물의 항염증 및 미백효능 검증

장영아 한국응용과학기술학회 2018 한국응용과학기술학회지 Vol.35 No.2

본 연구는 화장품 소재로서 인삼복합물의 가능성을 확인하기 위한 것이다. 이를 위해 우리는 인삼복합물 에탄올 추출물을 사용하여 항염증, 미백 효과에 대한 생물학적 활성 평가를 수행하였다. 시료는 인삼(A), 상황(B), 리기다소나무(C)를 각 70% 에탄올로 추출한 후 (A)1 : (B)1 : (C)0.5 의 비율로 혼합한 시료를 사용하였다. 대식세포(RAW 264.7 cells)내에서 시료의 항염증 효과를 평가하기 위해 MTT assay를 이용한 샘플의 독성평가와 nitric oxide 생성 저해 활성 및 염증관련 단백질 및 유전자의 발현량을 확인하였다. 시료의 미백 효과를 평가하기 위해서 멜라노마(B16F10 cell)를 이용해 MTT assay를 통한 샘플의 독성평가, cellular tyrosinase 저해율 측정 및 멜라닌 생합성량을 측정하였다. LPS 로 유도된 RAW 264.7 세포내에서 시료의 nitric oxide 저해 활성 결과는 25 µg/mL 농도에서 71.2%의 우수한 효능을 나타내었으며 western blot 실험결과 iNOS, COX-2 단백질의 발현은 농도 의존적으로 감소하는 것을 확인하였다. Tyrosinase 활성 억제 실험에서 인삼복합물의 농도 50 µg/mL 에서 36.8% 의 감소를 나타냈으며 멜라닌 생합성 억제 평가 결과에서 농도 50 µg/mL 에서 47.8%의 감소를 나타냈 다. 상기 실험 결과로부터 인삼복합물의 우수한 항염증, 미백 효능을 확인하였으며 향후 안전한 천연 화장품 원료로 사용될 수 있음을 확인하였다. This study is for checking the possibility of ginseng complex as cosmetic materials. For this we carried out biological active evaluation about anti-inflammatory and whitening effects by using ethanol extract of ginseng complex. Samples were prepared by extracting 70% ethanol from each of Panax ginseng C. A. Meyer (A), Phellinus linteus (B) and Pinus rigida Mill. (C), and mixing them at a ratio of (A) 1 : (B) 1 : (C) 0.5. In order to evaluate the anti-inflammatory effects of the samples in macrophages (RAW 264.7 cells), MTT assay was used to evaluate the toxicity of the samples and the inhibitory activity of nitric oxide production and the expression levels of inflammation-related proteins and genes. To evaluate the whitening effect of the samples in melanoma (B16F10 cell), MTT assay was used to evaluate the toxicity of the sample, cellular tyrosinase inhibition, and melanin contents. The inhibitory activity of nitric oxide in the LPS-induced RAW 264.7 cells was 71.2% at 25 μg/mL concentration and western blot analysis showed that the expression of iNOS and COX-2 protein decreased in a concentration-dependent manner. Inhibition of tyrosinase activity showed 36.8% inhibition at 50 μg/mL concentration of ginseng complex and inhibition of melanin contents showed 47.8% inhibition at 50 μg/mL concentration. From the results of the experiment, it was confirmed that the ginseng complex had excellent anti-inflammatory and whitening effect and could be used as a safe natural cosmetic material in the future.

청소년의 취약성 자기애가 발표불안에 미치는 영향: 무조건적 자기수용과 공적자의식의 매개효과

장영아,권경인 한국청소년상담복지개발원 2021 청소년상담연구 Vol.29 No.1

The purpose of this study was to examine the relationship samong vulnerable narcissism, unconditional self-acceptance, public self-consciousness, and speech anxiety in adolescents. It also seeks to identify the mediating effects of unconditional self-acceptance and public self-consciousness on the association between vulnerable narcissism and speech anxiety. Structural equation modeling was conducted to analyze the data from 479 middle and high school students in Seoul that were collected using speech anxiety scale, vulnerable narcissism scale, unconditional self-acceptance scale, and public self-consciousness scale. Results showed that unconditional self-acceptance and public self-consciousness had sequential mediating effects on the association between vulnerable narcissism and speech anxiety. These results suggest that adolescents with high vulnerable narcissism experience difficulties in unconditionally accepting themselves. This increases public self-consciousness that leads to excessive attention to external assessment, raising anxiety in a certain situation, i..e, giving a speech. This study is significant in that it revealed therapeutic variables such as unconditional self-acceptance and public self-consciousness that can intervene in the direct connection between vulnerable narcissism and speech anxiety. 본 연구의 목적은 청소년의 취약성 자기애, 무조건적 자기수용, 공적자의식, 발표불안의 관계를 살펴보고 취약성 자기애와 발표불안의 관계에서 무조건적 자기수용과 공적자의식의 매개효과를 확인하고자 하였다. 이를 위해 서울 소재 4곳의 중․고등학생 479명을 대상으로 발표불안 척도, 취약성 자기애 척도, 무조건적 자기수용 척도, 공적자의식 척도를 사용하여 수집된 자료를 바탕으로 구조방정식 모형을 통해 검증하였다. 그 결과를 요약해보면 다음과 같다. 취약성 자기애와 발표불안의 관계에서 무조건적 자기수용과 공적자의식이 순차적으로 매개효과가 유의했다. 이러한 결과는 취약성 자기애가 높은 청소년은 아무 조건 없이 무조건적으로 자기를 수용하는데 어려움을 겪게 되며 이로 인해 외부의 평가에 지나치게 주의를 기울이게 되는 공적자의식이 상승하게 되어 발표하는 특정 상황에서 불안이 높아짐을 의미한다. 이러한 결과를 바탕으로 취약성 자기애가 발표불안으로 직접 이어지지 않을 수 있도록 무조건적 자기수용 및 공적자의식 변인을 함께 고려함으로써 발표불안에 개입할 수 있는 치료적 변인을 밝힌 것에 의의가 있다.

블루베리, 상황, 율피, 승마 복합추출물의 화장품 소재로서의 효능

장영아 한국피부과학연구원 2017 아시안뷰티화장품학술지 Vol.15 No.3

Purpose: To confirm the possibility of using complex extracts of Vaccinium spp., Phellinus linteus, Castanea crenata, and Cimicifuga heracleifolia for cosmetic substances, we tested anti-oxidant and anti-inflammatory activities. Methods: The complex extracts of Vaccinium spp., Phellinus linteus, Castanea crenata, and Cimicifuga heracleifolia were used in a ratio of 1:1:1:1, and these complexes were extracted in 70% ethanol. To evaluate the anti-oxidant activity, the complexes were concentrated and the scavenging activities of the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and 2,2’-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) cation were assessed. The anti-inflammatory activity was measured using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, western blot analysis, and real-time polymerase chain reaction (real-time PCR). Results: Complex extracts showed 83.1% DPPH and 98.4% ABTS+ radical scavenging activities at a concentration of 1000 µg/mL. The MTT assay revealed cell viability over 81.0% at 10, 25, and 50 µg/mL concentrations of the complex extracts and cell death over 21.0% at 100 and 250 μg/mL concentrations of the complex extracts. Therefore, the experimental concentration of the complex extracts was 10–50 μg/mL. Western blot analysis revealed that at 50 µg/mL concentration of the complex extracts, the expression level of nitric oxide (NO) was decreased by 28.0% and those of inducible nitric oxide synthase (iNOS) and cyclooxygenase 2 (COX2) were decreased by 32.8% and 48.0%, respectively. Real-time PCR showed a decrease in iNOS and COX2 mRNA expression levels in a concentration-dependent manner. Conclusion: Complex extracts have excellent anti-oxidant and anti-inflmmatory effects, and we expect that these extracts will serve as functional cosmeceutical materials. 목적: 본 연구에서는 블루베리, 상황, 율피, 승마 복합추출물의 화장품 소재로서의 가능성을 확인하기 위해 항산화와 항염증 활성을 측정하였다. 방법: 블루베리, 상황, 율피, 승마는 70% 에탄올에서 추출하였고, 복합추출물은 각 시료를 1:1:1:1의 비율로사용하였다. 항산화 평가를 위해 복합물을 농도에 따라 처리하여 1,1-diphenyl-2-picrylhydrazyl (DPPH) 라디칼 소거능과 2,2’ -azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) 양이온 라디칼 소거능을 확인하였다. 항염증 활성평가는 과학적으로 증명된 방법인 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) 분석법, western blot 분석법, realtime polymerase chain reaction (real-time PCR) 분석법으로 측정하였다. 결과: 복합추출물은 1000 µg/mL 농도에서 DPPH 라디칼 소거능 활성이 83.1%로 나타났으며 ABTS+ 라디칼 소거능 활성이 98.4%로 나타났다. MTT 분석법을 통한 세포 생존율은 10, 25, 50 µg/mL 농도에서는 생존율이 81.0% 이상으로 나타났고, 100, 250 µg/mL 농도에서는 세포사멸이 21.0% 이상으로 나타났다. 따라서 복합물의 최종농도를 10–50 µg/mL로 사용하였다. Nitric oxide (NO) 생성은 50 µg/mL 농도에서 28.0% 감소되었고, western blot 분석을 통한 inducible nitric oxide synthase (iNOS)와 cyclooxygenase 2 (COX2) 단백질 발현은 50 µg/mL 농도에서 각32.8%, 48.0%의 감소를 나타내었다. Real-time PCR 결과iNOS와COX2의 mRNA 발현율은 농도 의존적으로 감소되었다. 결론: 복합추출물은 항산화 및 항염증에 탁월한 효능이 있으며 이러한 추출물이 향후 약용화장품 소재로서 사용되기를 기대한다.

홍삼추출물이 LPS로 유도된 RAW 264.7 cell의 염증반응에 미치는 효과

장영아,김한나,김보애 한국응용과학기술학회 2019 한국응용과학기술학회지 Vol.36 No.4

We conducted this study to investigate anti-inflammatory possibilities of applying cosmetic material about extracts from red ginseng. For this we carried out biological active evaluation about anti-inflammatory by using extracts of red ginseng. In order to evaluate the anti-inflammatory effects of the samples in macrophages (RAW 264.7 cells), MTT assay was used to evaluate the toxicity of red ginseng extracts and the inhibitory activity of nitric oxide production and the expression levels of inflammation-related proteins and genes. The inhibitory activity of nitric oxide in the LPS-induced RAW 264.7 cells was 71.2% at 25 μg/ml concentration and western blot analysis showed that the expression of iNOS and COX-2 protein decreased in a concentration-dependent manner. These results suggest that extracts from red ginseng may have value as the potential cosmetic materials. 본 연구는 홍삼추출물의 화장품소재로서의 항염증 효과의 가능성을 확인하는 것을 목적으로 한다. 본 연구에서는 홍삼 추출물을 사용하여 항염증에 대한 생물학적 활성평가를 수행하였다. 대식세포인 RAW 264.7 세포에서 시료의 항염증 효과를 평가하기 위해 MTT assay를 이용한 홍삼 추출물의 독성평가와 nitric oxide 생성 저해 활성 및 염증관련 단백질 및 유전자의 발현량을 확인하였다. LPS로 유도된 RAW 264.7 세포 내에서 시료의 nitric oxide 저해활성은 25 μg/ml에서 71.2 %의 우수한 효능을 나타내 었으며, western blot 시험결과 iNOS, COX-2 단백질의 발현은 농도 의존적으로 감소하는 것으로 확인하 였다. 이러한 실험 결과를 바탕으로 홍삼추출물이 항염 효과를 가진 화장품 소재로서의 가치를 제안할 수있다.

추출용매와 부위별 복령(Poria cocos )의 항산화, 항주름, 항염증 평가

장영아,이진태 대한피부미용학회 2015 대한피부미용학회지 Vol.13 No.3

This study is for checking the possibility of Poria cocos as cosmetic materials. For this we carried out biological active evaluation about antioxidant, anti-inflammatory and anti-aging by using extraction parts of Poria cocos and solvent extraction. We extracted Poria cocos by extraction parts (bark, inside skin) and solvents (water extracts, ethanol), and then in order to evaluate antioxidant activity we processed samples by concentration and carried out DPPH radical scavenging and ABTS radical scavenging. Also, in order to evaluate effect of anti-aging we carried out evaluation of Elastase inhibitory activity and Collagenase inhibitory activity. To evaluate effect of anti-inflammatory we evaluated toxicity of samples through MTT assay with a macrophage (raw 264.7 cells) and measured Nitric Oxide production inhibitory activity. As a result at the experiment of DPPH radical scavenging and ABTS radical scavenging of antioxidant evaluation, ethanol extracts showed more superiority in all three groups, A, B and C than water extracts of Poria cocos of evaluations of the effect against wrinkles, according to the result of measurement of elastase inhibitory activity, PCE (A) showed 96.7% effect and PCE(B) showed 98.3% effect respectively at 1000μg/ ml concentration. Also, according to the result of measurement of collagenase inhibitory activity, PCE (A) showed 60.0% effect and PCE (B) showed 72.2% effect respectively, so it means effect of ethanol extracts is superior. According to the result of nitric oxide production inhibitory activity, PCW (A) showed 60.4% effect and PCW (B) showed 60.3% effect respectively. This showed that effect of anti-inflammatory was greater in water extracts. In other words, ethanol extracts of Poria cocos proved effective against wrinkles by hindering antioxidant capacity and activities of elastase and collagenase by ROS. Water extracts showed superiority of effect of anti-inflammatory, so it suggests that Poris cocos extracts can be used as natural substance of cosmetics which are safe in antioxidant, anti-inflammatory, and anti-aging.

오이풀 분획물의 화장품 소재로서의 약리활성 연구

장영아,천순주,배해병,이지현,이진태 한국임학회 2012 산림과학 공동학술대회 논문집 Vol.2012 No.-

한방복합물의 화장품 소재로서의 약리활성 연구

장영아,전동하,이지현,권용진,이재봉,정수연,이진태 한국산림휴양복지학회 2013 한국산림휴양학회 학술발표회 자료집 Vol.2013 No.4

학술연구논문 구두발표 2 : 제주산 알로에의 화장품 소재로서의 약리활성 연구

장영아,김지향,심효은,장민정,이진태 한국임학회 2013 산림과학 공동학술대회 논문집 Vol.2013 No.-

Contrast-induced Nephropathy in Major Trauma Patients

장영아,박정호,최승필,위정희 대한응급의학회 2017 대한응급의학회지 Vol.28 No.1

Purpose: Occult injuries are diagnosed using computed tomography (CT), often with intravenous (IV) contrast CT, in major trauma patients. The contrast dye is known to be potentially nephrotoxic. Thus, we measured the incidence and risk factors for contrast-induced nephropathy (CIN) in major trauma patients. Methods: A retrospective review was performed on 247 patients who were treated by the activated trauma team between June 2012 and July 2014. The exclusion criteria were underlying renal failure, no IV contrast CT administered, and no creatinine (Cr) follow-up within 72 hours. We examined age, gender, initial vital signs, the Glasgow Coma Scale (GCS), initial Cr levels, Cr level within 72 hours after admission, the IV contrast CT number, Injury Severity Score (ISS). CIN was defined as 25% relative or 0.5 mg/dL absolute increase in serum Cr within 72 hours of presentation. Results: One hundred forty-three patients were included in this study. CIN manifested in 12 patients (8.4%). The injury severity was associated with CIN (ISS≥16, p=0.003; GCS≤8, p<0.001; death, p<0.001). Initial Cr levels, elderly patients, and initial hypotension were not statistically significant (p=0.065, 0.176, and 0.402, respectively). The number of IV contrast CTs administered was also not associated with the occurrence of CIN (p=0.331). Conclusion: We found an 8.4% incidence of CIN in multiple trauma patients exposed to IV contrast. The number of IV contrasts, age, initial systolic blood pressure, and initial Cr levels were not associated with CIN. Only injury severity was associated with CIN occurrence; therefore, physicians should pay more attention to severely-injured patients.

고온이력을 받은 섬유 혼입 콘크리트의 잔존 역학적 특성 사례분석

장영아,김원창,임하준,정기신,이태규 한국화재소방학회 2024 한국화재소방학회 학술대회 논문집 Vol.2024 No.1