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천순주,김영훈,황연경,이진태,박경숙,조우아,최은영 한국미용학회 2007 한국미용학회지 Vol.13 No.1
In the anti-oxidant experiment, radical-scavenging activity of water and ethanol extract was examined using 1,1-diphenyl-2-picrylhydrazyl radicals. Water and ethanol extract from Taraxacum platycarpum showed 62.9% and 54.7% at 500ppm concentration in DPPH radical inhibition, respectively. The emulsion containing Taraxacum platycarpum microcapsule were stable at temperature conditions(0℃, 25℃, 40℃), sun lamp test, sun-light and cycle chamber, during 28 days. The present study suggested that Taraxacum platycarpum microcapsule have a potential as a new slow release cosmetic material. From the result of human patch test to assess the safety showed no stimulus of negative reaction on skin. As for the Taraxacum platycarpum, physiology activity is excellent as things mentioned above, and it is done an idea because safety is identified as safety, and emulsion including a Taraxacum platycarpum microcapsule has the use on cosmetics development. 1. 포공영 추출물의 항산화 효과를 실험한 결과, 10,000ppm의 열수 추출물은 68.5%, 에탄올 추출물은 74%의 전자공여능을 나타내었다. 2. 포공영을 마이크로캡슐을 함유한 emulsion의 인공광과 자연광 노출에 따른 광안정성 실험결과 변색, 발색, 변취, 산패 등의 변화를 일으키지 않고 안정하였다. 3. 포공영을 마이크로캡슐을 함유한 emulsion의 온도 안정성을 실험에서도 변색, 변취, 응집 등의 불안정 상태가 나타나지 않았다.
천순주(Soon-Ju Cheon),장민정(Min-Jung Jang),장영아(Young-Ah Jang),최은영(Eun-Young Choi),전동하(Dong-Ha Jun),김영훈(Young-Hun Kim),조우아(Woo-A Cho),정연숙(Yeon-Sook Jeong),권혁범(Hyeork-Bum Kwon),김태훈(Tae-Hoon Kim),최경임(Kyung-Im 한국생명과학회 2008 생명과학회지 Vol.18 No.12
사람의 피부는 자외선, 오염된 공기, 화학 제품 등 환경에 끊임없이 노출된다. 이들로 인한 자유라디칼과 활성 산소는 피부세포에 큰 손상을 끼치게 된다. 캄보디안 상황버섯 열수, 에탄올 추출물의 기능성 화장품 소재로 활용하기 위하여 지방 산패 억제능 측정과 주름개선 효과 측정을 하였다. 캄보디안 상황버섯 열수, 에탄올 추출물에 금속이온을 첨가하여 지방산패 억제능을 측정한 결과 캄보디안 상황버섯 열수 추출물 보다 에탄올 추출물이 Fe²?와 Cu²?chelating 능력이 우수하였다. 캄보디안 상황버섯 에탄올 추출물의 경우 0.1 ㎎/㎖, 0.5 ㎎/㎖, 1 ㎎/㎖의 농도에서 대조군인 butylated hydroxytoluene (BHT)보다 높은 지방산패 억제를 나타내었다. 캄보디안 상황버섯 열수, 에탄올 추출물은 산화 촉매제인 Fe²를 함유한 지방 산패 억제능이 Cu²?를 이용한 지방산패 억제능 보다 우수하였다. 주름개선 효과 측정으로 elastase 저해활성을 측정한 결과 캄보디안 상황버섯 에탄올 추출물은 0.01 ㎎/㎖의 농도에서 50.7%의 저해활성을 나타내어, 대조군인 urosolic acid보다 높은 elastase 저해활성을 타나내었다. 또한 에탄올 추출물의 경우 0.1 ㎎/㎖의 농도에서 약 50%의 저해활성을 나타내어 캄보디안 상황버섯 열수 추출물 보다 에탄올 추출물이 collagenase에 대해 높은 저해활성을 나타내었다. 이상의 결과에서 캄보디안 상황버섯의 에탄올 추출물은 지방산패 억제능이 우수할 뿐만 아니라 주름 개선 효과가 있음을 확인할 수 있었고, 주름 개선 화장품 소재로서의 가능성을 확인할 수 있었다. The skin of human is constantly being exposed to environmental irritants such as ultraviolet, smoke and chemicals. These irritants cause free radicals and reactive oxygen species which leave serious damages on the cells of skin. The water and ethanol extracts of Cambodian Phellinus linteus were investigated for the activities of anti-lipid peroxidation and anti-wrinkle effects to apply as a functional ingredient for cosmetic products. As the result of evaluation of liquid oxidation rate by add Fe²? and Cu²? to Cambodian Phellinus linteus extracts, Cambodian Phellinus linteus ethanol extracts were higher than Cambodian Phellinus linteus water extracts in the chealting ability of Fe²? and Cu²?. The Cambodian Phellinus linteus ethanol extracts exhibited that anti-lipid peroxidation higher than butylated hydroxytoluene (BHT) at the concentration of 0.1 ㎎/㎖, 0.5 ㎎/㎖ and 1 ㎎/㎖. Cambodian Phellinus linteus water and ethanol extracts showed a higher inhibitory effect on Fe²?-induced lipid peroxidation compared to Cu²?-induced lipid peroxidation. In the case of anti-wrinkle effect, the elastase inhibition activity of Cambodian Phellinus linteus ethanol extracts was 50.7%, and it is higher than urosolic acid at the concentration of 0.01 ㎎/㎖. Also, in collagenase inhibition activity, Cambodian Phellinus linteus water extract showed low effect, but Cambodian Phellinus linteus ethanol extract was about 50% at a 0.1 ㎎/㎖. concentration. These results proved that Cambodian Phellinus linteus had anti-lipid peroxidation and anti-wrinkle effect. Therefore, Cambodian Phellinus linteus could be useful as an anti-wrinkle cosmetic ingredient.
4가지 한방 소재(행인, 호장근, 자초, 강황)의 항산화활성에 관한 연구
김영훈,이수미,천순주,장민정,전동하,최향자,조우아,이진태 한국패션뷰티학회 2007 한국패션뷰티학회지 Vol.5 No.4
There was an increasing interest that herbal medicine and natural material extracts were proved processes of antioxidant, cosmeceutical activity and the other effects. The aim of this study was to assess the antioxidant of extraction of four kinds from Prunus armeniaca L., Reynoutria elliptica, Curcuma aromatica, Lithospermum erythrorhizon. RE (Reynoutria elliptica) and CA (Curcuma aromatica) have good electron donating ability. The water and ethanol extract of RE at a 100 ppm concentration showed over 70%, the water extract at 500 ppm concentration showed 83% and the ethanol extract at 100 ppm concentration showed 86% of CA. Xanthine oxidase inhibition activity of the water extract of LE (Lithospermum erythrorhizon) at a 1,000 ppm concentration showed over 44%, on the other hand, RE showed in all lowest effect and there was no inhibition activity of a couple more extracts. In the measurement of nitrite scavenging activity, all extracts showed highly scavenging activity. Especially the water and ethanol extract of RE showed over 99% at 500 ppm, also LE showed over 40% at 10 ppm concentration.
4가지 한방 소재(행인, 호장근, 자초, 강황)의 화장품약리활성에 관한 연구
김영훈,이수미,천순주,장민정,전동하,최향자,조우아,이진태 한국패션뷰티학회 2007 한국패션뷰티학회지 Vol.5 No.4
The aim of this study was to assess the cosmeceutical activies of four kinds of Korea herb medicine extracts using in cosmetics and related industries. The cosmeceutical activities of extracts were investigated by tyrosinase inhibition, astringent, anti-bacterial and MTT assay for cell viability. In the whitening effect, PA that the highest tyrosinase inhibition activity showed 56% at 10 ppm in ethanol extract. Also water and ethanol extract of RE showed 54%, 68% at 1,000 ppm, respectively, but LE and CA showed lower effect. Astringent effect of water and ethanol extract of PA appeared over 60% at 1,000ppm concentration but other extracts showed no astringent effect. In the anti-bacterial test, water and ethanol extract of PA showed no anti-bacterial effect against all microorganisms. But water and ethanol extract of RE showed anti-bacterial effect on Staphylococcus aureus, ethanol extract of showed on Staphylococcus aureus and Propionibacterium acnes, ethanol extract of CA showed on Candida albicans. The resUlt of stability test showed that the emulsion of containing PA were very stable at various temperature and sun-light test. Viscosity and pH of emulsion did not change. From the results of human patch test to assess the safety of cosmetics containing PA there was no negative reaction on skin was found.
음나무(Kalopanax septemlobus Koidz.) 잎 추출물의 폴리페놀 및 항산화효과
전동하,이진태,천순주,이창언,김태훈,이도형,한진규,김세현,Jun, Dong-Ha,Lee, Jin-Tae,Cheon, Soon-Ju,Lee, Chang-Eon,Kim, Tae-Hoon,Lee, Do-Hyung,Han, Jin-Gyu,Kim, Sea-Hyun 한국자원식물학회 2009 한국자원식물학회지 Vol.22 No.4
음나무 잎 추출물의 생리활성을 분석하기 위해 열수추출물과 70% EtOH 추출물의 폴리페놀의 함량을 측정하였으며, 항산화활성 분석을 위해 전자공여능, nitric oxide radical 저해활성, xanthine oxidase 저해활성, ABTS radical cation decolorization을 측정하였다. 폴리페놀의 총 함량은 열수추출물과 70% EtOH 추출물에서 각각 104 mg/L, 125 mg/L으로 정량되어 70% EtOH 추출물이 열수추출 물에 비해 높은 함량을 보였다. DPPH radical 소거능은 70% EtOH 추출물이 1,000 ppm에서 93.1%로 나타나 같은 농도의 BHA와 유사한 소거능을 보였다. nitric oxide radical 저해활성은 70% EtOH 추출물과 열수추출물이 1,000 ppm에서 49.6%, 49.5%로 나타나 같은 농도의 BHA보다 높은 nitrit oxide radical 저해활성을 보였다. Xanthine oxidase 저해활성은 1,000 ppm농도에서 열수 추출물과 70% EtOH 추출물에서 각각 46.6%, 60.4% 활성을 나타내었다. ABTS radical cation decolorization 활성은 1,000 ppm에서 열수추출물이 92.7%, 70% EtOH 추출물이 91.4%로 나타났다. 이상의 결과로 보아 음나무 잎의 70% EtOH 추출물과 열수추출물 모두 많은 폴리페놀이 함유되어 있으며, 70% EtOH 추출물과 열수추출물 모두 합성항산화제에 비해 항산화 활성이 다소 낮지만 천연 항 산화제로 우수한 항산화활성을 나타내는 것으로 분석되었다. 이를 바탕으로 음나무 잎의 강한 항산화능을 피부노화 예방관련 미용식품 및 기능성 화장품 소재로 활용 가능한 유용한 수종으로 개발 될 수 있을 것이다. The aim of the study was to assess the cosmeceutical activity of Kalopanax septemlobus leaf and it is possible that can be used as a cosmetic ingredient for applicaiton of cosmetic industries. The concentration of total phenolic compound of hot water and 70% EtOH extracts of K. septemlobus leaf showed 104 mg/L and 125 mg/L respectively. In the result of DPPH(1,1- diphenyl-2-picryl -hydrazyl) scavenging radical activity, 70% EtOH extracts of K. septemlobus leaf showed 93.1% and it was similar to BHA(butylated hydroxyanisole) effect at 1,000ppm concentration. Xanthine oxidase inhibition of hot water extracts and 70% EtOH extracts of K. septemlobus leaf were 46.6% and 60.4% at 1,000ppm, respectively. In these results, K. septemlobus leaf has a great potential as a cosmeceutical ingredient with a natural anti-oxidant source.
토사자( Cuscuta japonica Choisy, 토絲子)를 이용한 한방팩의 안전성과 피부상태 개선에 관한 연구
조우아 ( Woo A Cho ),천순주 ( Soon Ju Cheon ),이진태 ( Jin Tae Lee ) 한국미용학회 2008 한국미용학회지 Vol.14 No.3
This research is written to study safety and improvement of skin condition of C. japonica Choisy when used as the ingredient of pack was tested. The C. japonica Choisy sample was prepared as the extract of water and ethanol C. japonica Choisy is seed of vine characteristic plant that is in the family of the bindweed. Problems such as a change of color and separation of phase were not discovered in sun-lamp test. As the result of the clinical demostration of C. japonica Choisy water extract contained pack, the trivial change in skin`s pH and the moisturizing effect in proportion to the number of times using the pack were observed. In the survey of the pack containing C. japonica Choisy water extract, parts of whitening, moisturizing and purchasing desire scored high, however, the parts of color, texture, and scent need to be improved. Furthermore, by the result of the patch test, it was showed that there was no stimulus on skin when using the pack containing C. japonica Choisy water extract, which proves the safety of using C. japonica Choisy as the ingredient of the cosmeceutical activity. According to all the test results that have been given, it is possible that the extract of C. japonica Choisy can be used as a new natural material of oriental herbal cosmetics.