상피성 난소종양에서 MTA1 발현에 관한 연구

윤만수 ( Man Soo Yoon ),장성규 ( Sung Kyu Jang ),이동형 ( Dong Hyung Lee ),김기형 ( Ki Hyung Kim ),나용진 ( Yong Jin Na ),김활웅 ( Hwal Woong Kim ),김지연 ( Jee Yeon Kim ),최경운 ( Kyung Un Choi ) 대한산부인과학회 2006 Obstetrics & Gynecology Science Vol.49 No.7

목적: 상피성 난소종양을 대상으로 조직배열법을 이용한 면역조직화학염색을 실시하여 여러 임상병리학적 인자에 따른 MTA1발현의 상관관계를 알아보고자 하였다. 연구 방법: 양성 종양 53예, 경계성 종양 27예, 악성 종양 68예를 대상으로 면역조직화학염색을 시행하여 MTA1의 발현을 판정하였고, 악성 종양의 여러 임상병리학적 인자에 따른 MTA1의 발현의 차이를 분석하였다. 결과: 종양의 악성도 등급별 MTA1 염색의 성적은 악성도가 높을 수록 MTA1의 고발현 예가 많고 양성 종양에서는 저발현 예가 많아 유의한 차이를 보였고, 종양의 병기가 높을 수록 MTA1의 발현이 증가하였으며, 특히 원격전이를 보이는 4기의 경우 모두가 고발현군이었다. 그 외 환자의 나이, 조직학적 분류, 종양의 크기에 따른 MTA1 발현 정도에는 유의한 차이는 없었다. 결론: 이러한 결과들을 토대로 난소종양에서 MTA1은 종양의 침습과 공격성을 결정하는 주요한 인자일 것으로 추측할 수 있었으며 난소암의 치료나 예후에 중요한 정보를 제공할 수 있을 것으로 사료된다. Objective: MTA1 has been identified as a metastasis-promiting gene, and its gene expression is correlated with invasion and metastasis in several cancers. We examined MTA1 expression levels in epithelial ovarian neoplasm. Methods: Expression of MTA1 was evaluated by immunohistochemistry and tissue array in 53 benign tumors, 27 borderline tumors and 68 malignant tumors. The data was analyzed in reference to various clinicopathological parameters. Results: Increased expression of MTA1 was significantly correlated with histologic grade and FIGO stage. There was no relationship between MTA1 expression and age, histologic type, tumor size. Conclusion: These results suggest that MTA1 is closely related to invasiveness and progression in epithelial ovarian neoplasm. The MTA1 could thus potentially provide information on the mechanism of cancer invasion and metastasis.

자궁경부 원추절제술 후 시행한 자궁경관내 세포진 검사의 역할에 관한 연구

윤만수(Man Soo Yoon),이규섭(Kyu Sup Lee),최욱환(Ook Hwan Choi),김원희(Won Whe Kim) 대한산부인과학회 2000 Obstetrics & Gynecology Science Vol.43 No.6

목적 : 자궁경부 원추절제술 후 잔여병변을 예측하는 방법으로서 자궁경관 세포진 검사의 효용성을 알아보고자 연구를 시행하였다. 방법 : 자궁경부의 고도의 상피내 종양이나 미세침윤암으로 원추절제술 시행 후 자궁적출술을 시행한 229명의 환자를 대상으로 원추절제된 조직의 절단경계면의 상태, 원추절제술 후 시행한 자궁경관 세포진 검사의 결과와 자궁적출술 후의 조직에서의 잔여병변과의 연관성을 조사하였다. 결과 : 원추절제술 후 절단경계면의 상태와 잔여병변의 관계에서 절단경계면에 병변의 침범이 있었던 경우 52.6 % (41/78)에서 자궁적출술 후 잔여병변이 발견되었으나 절단경계면에 병변의 침범이 없었던 경우 8.6 % (13/151)에서만이 잔여병변이 발견되었다. 원추절제술 직후 시행한 자궁경관 세포진 검사 결과에 따라서는 세포진 검사가 양성인 경우 76.5 % (26/34)에서 잔여병변이 발견된 반면 세포진 검사가 음성인 경우는 14.4 % (28/195)에서만이 잔여병변이 발견되었다. 결론 : 원추절제술 후 잔여병변의 예측을 위해 자궁경관의 상태를 파악하는데 있어 자궁경관 소파술 대신 자궁경관의 세포진 검사를 시행하는 경우도 잔여병변의 예측에 효율적인 방법이 될 수 있을 것으로 사료된다. Objective : To evaluate the impact of the cytologic smear of the endocervical canal immediately after cervical conization on the rate of residual cervical lesions. Method : A retrospective review of 229 patients who underwent cervical conization for high grade cervical intraepithelial neoplasia or microinvasive cancer followed by hysterectomy. Endocervical Papanicolaou smear with a cytologic brush performed immediately after cervical conization in all 229 patients. Results : Fifty-four of 229 patients undergoing cervical conization followed by hysterectomy had residual lesion in the hysterectomy specimen. The prevalence rate of residual lesion according to the margin status of conization specimen as well as the results of the postconization Papanicolaou smear of the endocervical canal was compared. Forty-one of 78 patients (52.6 %) with positive margins had residual lesion compared with thirteen of 151 patients (8.6 %) with negative margins. Twenty-six of 34 patients (76.5 %) with positive postcone cytology had residual lesion compared with twenty-eight of 195 patients (14.4 %) with negative postcone cytology.Conclusion : Postcone cytologic smear of the endocervical canal as well as margin status are useful in predicting residual lesion after conization.

폐경 여성에서 타목시펜 사용 후 발견된 거대 자궁내막용종

문수현 ( Soo Hyeon Moon ),이성의 ( Seong Eui Lee ),정인국 ( In Kook Jung ),정주은 ( Ju Eun Jeong ),박원영 ( Won Young Park ),이우희 ( Woo Hee Yi ),서동수 ( Dong Soo Suh ),윤만수 ( Man Soo Yoon ),김기형 ( Ki Hyung Kim ) 대한산부인과학회 2011 Obstetrics & Gynecology Science Vol.54 No.12

Tamoxifen is a synthetic non-steroid anti-estrogen that has been used effectively for several years in the adjuvant treatment of breast cancer. But, the drug has been associated with development of endometrial poylp, hyperplasia and adenocarcinoma possibly mediated through its agonistic estrogen properties during the menopausal period in which estrogens are at a low level. Endometrial polyp has been described as the most common endometrial pathology in association with postmenopausal tamoxifen treatment. We present the case of woman with a giant endometrial polyp of uncommon dimension who was receiving adjuvant tamoxifen for 5 years after breast cancer surgery.

상피성 난소암 세포에서 MyD88 발현과 paclitaxel의 항세포고사 신호

서동수 ( Dong Soo Suh ),조무성 ( Moo Sung Jo ),유신애 ( Shin Ae Yu ),김기형 ( Ki Hyung Kim ),윤만수 ( Man Soo Yoon ) 대한산부인과학회 2010 Obstetrics & Gynecology Science Vol.53 No.4

목적: 상피성 난소암 세포를 Myeloid differentiation protein 88 (MyD88) 발현에 따라 구별하고 MyD88 발현이 paclitaxel의 항종양 효과에 어떤 영향을 미치는지, 또 paclitaxel이 난소암 세포에서 어떤 항세포고사 신호를 유도하는지를 알아보고자 하였다. 연구 방법: 난소암 환자의 복수에서 분리한 일차세포와 확립되어진 난소암 세포주를 각각 paclitaxel (0.2~20 μM)로 24시간, 48시간동안 치료하였다. 세포 생존율은 CellTiter 96 AQueous One Solution Cell Proliferation Assay를 통해서, 사이토카인은 세포배양 후 배양액의 상층액을 채취하여 Luminex 200 system을 이용하여 측정하였고, nuclear factor-kappaB (NF-κB) 활성도는 Luciferase reporter system을 이용하였고, phospho-extracellular signal-regulated kinase (p-ERK)는 Western blot 분석을 하였다. 결과: MyD88 발현에 따라 A2780, R454, 01-28는 음성, R182, 01-19b, SKOV3는 양성으로 나누었다. MyD88 음성세포 (A2780, R454, 01-28)에서는 24시간 paclitaxel 치료 후 세포의 생존율이 각각 57%, 49%, 42%이었고, 48시간 치료 후에는 각각 35%, 28%, 29%를 보여 paclitaxel에 감수성을 나타내었다 (P=0.000). MyD88 양성세포 (R182, 01-19b, SKOV3)에서는 paclitaxel에 저항성을 나타내었다. Paclitaxel에 저항성을 보인 R182 세포에서는 caspase-3/7의 증가가 거의 없었으나 paclitaxel에 예민한 A2780 세포에서는 caspase-3/7의 뚜렷한 증가를 보였다 (P=0.000). MyD88 음성세포에서는 paclitaxel 치료 후 시간 경과에 따라 NF-κB 활성도가 감소하였으나 MyD88 양성세포에서는 NF-κB 활성도가 시간 경과에 따라 증가하였다. MyD88 양성인 R182 세포에서 interleukin-6 (IL-6)와 IL-8은 paclitaxel의 치료 농도에 비례하여 유의한 증가양상을 보였고, p-ERK의 기초 발현은 약하지만 paclitaxel 치료 후 p-ERK의 발현이 뚜렷하게 증가하였다 (P=0.000). 결론: Paclitaxel은 MyD88 양성인 난소암 세포에서 NF-κB를 활성화시키고, ERK 경로를 활성화시키며 염증성 사이토카인을 생성하는 항세포고사 신호를 나타내어 종양의 진행과 항암제내성에 관여하는 것으로 사료된다. Objective: The objectives of this study was to evaluate the correlation between myeloid differentiation protein 88 (MyD88) expression and paclitaxel effects on epithelial ovarian cancer cells and to evaluate whether paclitaxel had anti-apoptotic signals. Methods: Epithelial ovarian cancer cells isolated from ascites and established cell lines were treated with increasing concentrations of paclitaxel (0.2 to 20 μM) for 24 and 48 hours and cell viability was determined using the CellTiter 96 AQueous One Solution Cell Proliferation Assay. Cytokine profiling was performed from culture supernatants using the Luminex 200 system. Nuclear factor-kappaB (NF-κB) activity was determined using a Luciferase reporter system. Levels of phospho-extracellular signal-regulated kinase (p-ERK) were measured by Western blot analysis. Results: A strong signal for MyD88 expression was observed in R182, 01-19b and SKOV3 cells (MyD88-positive). A2780, R454 and 01-28 cells showed low levels of MyD88 (MyD88-negative). Paclitaxel effectively decreased cell viability in MyD88-negative A2780, R454, 01-28 cells after 24 and 48 hours (57%, 49%, 42% and 35%, 28%, 29%, respectively). MyD88-positive cells were resistant to paclitaxel. There was a significant increase in caspase-3/7 activity following paclitaxel treatment in MyD88-negative cells. No significant change in caspase-3/7 activity was detected in MyD88-positive cells. Paclitaxel induced NF-κB activation and enhanced the secretion of interleukin-6 (IL-6) and IL-8 in a dose dependent manner and induced ERK phosphorylation on MyD88-positive cells. Conclusion: Paclitaxel treatment for MyD88-positive ovarian cancer could have detrimental effects due to the paclitaxel-induced enhancement of NF-κB, ERK activities and pro-inflammatory cytokine production, which promote chemoresistance and tumor progression.

상피성 난소암세포에서 이소플라본 유도체 NV-196의 세포고사 유도 효과

서동수 ( Dong Soo Suh ),주종길 ( Jong Kil Joo ),김승철 ( Seung Chul Kim ),이동형 ( Dong Hyung Lee ),이우희 ( Woo Hee Yi ),윤만수 ( Man Soo Yoon ),김기형 ( Ki Hyung Kim ) 대한산부인과학회 2011 Obstetrics & Gynecology Science Vol.54 No.2

목적: 상피성 난소암세포(CP70, R182)에서 합성 이소플라본 유도체인 NV-196의 세포고사 유도효과를 조사하고, 이들 항암제에 내성을 보이 는 난소암세포에서 NV-196을 전처치하였을 때 세포고사 효과의 증강이 있는지를 알아보고자 하였다. 연구방법: 상피성 난소암세포인 CP70 세포와 R182 세포를 NV-196의 0.1, 1, 10 μg/mL의 농도로 24시간, 48시간 동안 치료하였다. 세포생존율은 CellTiter 96 AQueous One Solution Cell Proliferation Assay를 통해서, caspase 활성도는 Caspase-Glo assay를 이용하였다. 세포고사 경로의 확인을 위해 XIAP, caspase-2, Bid의 활성화는 western blot 분석을 하였다. 결과: NV-196은 CP70 세포와 R182 세포에서 모두에서 치료시간과 치료농도에 비례하여 세포생존율을 효과적으로 감소시켰다. 두 세포 모두에서 caspase-3의 유의한 상승이 관찰되어 세포고사에 의한 세포생존율의 감소임을 알 수 있었다. Caspase-8와 caspase-9도 양 세포에서 caspase-3의 증가와 유사한 양상을 보였다. Western blot 분석에서 XIAP의 불활성화와 caspase-2와 Bid의 활성화를 관찰하였다. 10 μg/mL NV-196을 8시간 동안 전처치하였을 때 CP70 세포와 R182 세포 모두에서 카보플라틴 또는 파클리탁셀로 치료하였을 때 세포생존율의 유의한 감소가 있었다. 결론: NV-196은 세포고사를 통해 유의한 세포생존율의 감소를 유도하였다. NV-196의 전처치는 난소암세포를 카보플라틴이나 파클리탁셀에 민감하게 만들어 항암제내성을 개선할 수 있을 것으로 기대된다. Objective: The objectives of this study were to determine the efficacy of NV-196, a synthetic isoflavone derivative, as a chemosensitizer in chemoresistant CP70 and R182 epithelial ovarian cancer (EOC) cells and to characterize the mechanism behind its sensitizing effect. Methods: EOC cells were treated with tenfold dilutions of NV-196 (0.1 to 10 μg/mL) for 24 and 48 hours. Cell viability was determined by the CellTiter 96 AQueous One Solution Cell Proliferation Assay. Apoptosis was assessed by Caspase-Glo assays and apoptotic cascade X-linked inhibitor of apoptosis protein (XIAP), caspase-2 and Bid were characterized by Western blot analyses. Results: As a monotherapy, NV-196 showed decreased cell viability in a time- and dose-dependent manner in both CP70 and R182 cells. A significant increase in caspase-3 activity was observed in both cells. Caspase-8 and -9 activation were also observed. Western blots demonstrated Bid and caspase-2 activation and cleavage of XIAP. NV-196 enhances the cytotoxic effects of carboplatin and paclitaxel. Conclusion: NV-196 induces cell death through the induction of apoptosis. Pretreatment with NV-196 may sensitize the ovarian cancer cells to carboplatin or paclitaxel. NV-196 may act as a chemosensitizing agent in epithelial ovarian cancer cells.

Expression of DNA-dependent Protein Kinase and Its Relationship with Epidermal Growth Factor Receptor Signaling in Metastatic Cancer Cell Lines

황지영,김선희,강치덕,윤만수,Hwang Jee Young,Kim Sun Hee,Kang Chi Dug,Yoon Man Soo Korean Society of Life Science 2005 생명과학회지 Vol.15 No.3

암세포의 유전적 불안정성은 부적절하게 활성화된 DNA수복경로와 관련되어 있다. 전이성 암은 높은 유전적 불안정성을 나타내는데, 이와 관련하여 본 연구에서는 전이성 암세포에서의 중요한 DNA수복 단백질의 하나인 DN의존성 단백질 키나아제(DNA-PK)의 발현 변화를 조사하였다. 여러 종류의 전이도가 다른 암세포들을 대상으로 한 실험에서 전이성 암세포들은 각각의 모세포에 비하여 DNA-PK 성분의 조절 소단위인 Ku70/80의 발현 및 Ku의 DNA 결합 활성이 증강되어 있었다. 또한 DNA-PK의 촉매 소단위인 DNA-PKcs의 발현 및 whole DNA-PK복합체의 kinase의 활성도 전이도가 큰 암세포에서 그 모세포보다 증강되어 있음을 알 수 있어, 전이성 암세포의 증강된 DNA수복능은 부적절한 DNA수복을 일으켜 암의 진행 및 전이를 촉진시키는 원인이 될 수 있음을 시사하였다. 한편 암세포의 표피성장인자수용체의 신호전달의 증강은 암의 침윤과 전이에 관련되어 있으며, DNA-PK의 기 기능에도 영향을 줄 수 있는 가능성이 보고 된 바 있는데, 본 연구에서는 표피성장인자수용체의 신호전달과 DNA-PK의 관련성을 명확히 밝히기 위하여 새로 개발된 EGFR tyrosine kinase inhibitor인 PKI166의 DNA-PK의 활성에 미치는 영향을 조사하였다. PKI166는 Ku70/80 및 DNA-PKcs의 발현을 억제하였고 이와 관련하여 전이성 및 항암제 다제내성 암세포에서 PKI166에 의하여 항암제에 대한 감수성을 증가시켜 항암제 내성을 나타내는 전이성 암세포 대한 치료법 연구에 DNA-PK가 분자적 표적이 될 수 있음을 밝혔다. The genetic instability of cancer cells may be related to inappropriately activated DNA repair pathways. In present study, the modulated expression of DNA-dependent protein kinase (DNA-PK), a major DNA repair protein, in human cancer metastatic cells was tested. The expressions of Ku70/80, regulatory subunit of DNA-PK, and the Ku DNA-binding activity in various highly metastatic cell lines were higher than those in each parental cell line. Also, the expression of DNA-PKcs, catalytic subunit of DNA-PK, and the kinase activity of the whole DNA-PK complex in highly metastatic cells were significantly increased as compared to those of parental cells, suggesting that the enhanced DNA repair capacity of metastatic cells could be associated with aberrant use of DNA repair, which may mediate tumor progression and metastatic potential. Increased EGFR (epidermal growth factor receptor) signaling has been associated with tumor invasion and metastasis, and the linkage between EGFR-mediated signaling and DNA-PK has been suggested. This study showed that PKI166, the new EGFR tyrosine kinase inhibitor, modulated the expressions of Ku70/80 and DNA-PKcs and also revealed the chemosensitization effect of PKI166 against metastatic cells may be in part due to inhibition of Ku70/80. These results suggest that interference in EGFR signaling by EGFR inhibitor resulted in the impairment of DNA repair activity, and thus DNA-PK could be possible molecular targets for therapy against metastatic cancer cells.

동결 전 단계적 노출처리방법이 유리화동결 및 초급속동결-융해 후 생쥐 성숙난자의 생존력에 미치는 영향에 관한 연구

김상우,이재익,김미경,이영아,이규섭,윤만수,Kim, Sang-Woo,Lee, Jae-Ik,Kim, Mi-Kyung,Lee, Young-Ah,Lee, Kyu-Sup,Yoon, Man-Soo 대한생식의학회 2000 Clinical and Experimental Reproductive Medicine Vol.27 No.2

Objective: This study was carried out to compare the effects of the stepwise exposure treatments on the morphological normality, fertilization and blastocyst formation rate of the frozen-thawed mouse mature oocytes by vitrification or ultra-rapid freezing and to use as a fundamental data for the cryopreservation of human oocytes. Materials and Methods: The morphological normality and fertilization rates of the vitrified and ultra-rapid frozen mouse mature oocytes after three-stepwise exposure treatments (1step, 3step and 5step) were observed. After choosing the 3step exposure treatment groups, we observed the morphological normality and fertilization, blastocyst formation rate of the vitrified and ultra-rapid frozen mouse mature oocytes. Results: The morphological normality and fertilization rates of the vitrified mouse mature oocytes after three-stepwise exposure treatments (1step, 3step and 5step) were 75%, 85%, 88% and 58%, 61 %, 54% respectively. There were no significant differences among treatments(p>0.05). The morphological normality and fertilization rate of the control was 92% and 65%. There were no significant differences in fertilization rate among control and treatments (p>0.05). The morphological normality and fertilization rates of the ultra-rapid frozen mouse mature oocytes after three-stepwise exposure treatments (1step, 3step and 5step) were 83%, 83%, 84% and 75%, 63%, 56% respectively. There were no significant differences among treatments (p>0.05). The morphological normality and fertilization rate of the control was 95% and 67%. There were no significant differences among control and treatments (p>0.05). The morphological normality and fertilization rate of the vitrified or ultra-rapid frozen mouse mature oocytes after 3step exposure treatment were 69% and 75%, respectively. The blastocyst formation rate was 60% and 57%. The results did not differ significantly between vitrification and ultra-rapid freezing (p>0.05). Conclusion: As known in the above results, there were no significant differences in the fertilization and blastocyst formation rate of the frozen-thawed mouse mature oocytes by vitrification or ultra-rapid freezing among the control and treatments. It is suggested that vitrification and ultra-rapid freezing method were effective for the cryopreservation of mouse mature oocytes.

자궁내막 증식증 및 선암에서의 간세포 성장인자와 c-Met의 발현과 혈관신생에 관한 연구

강기주(Gi Joo Kang),신병섭(Byoung Sup Sin),장성규(Seoung Kyu Jang),윤만수(Man Soo Yoon),이창훈(Chang Hoon Lee) 대한산부인과학회 2001 Obstetrics & Gynecology Science Vol.44 No.2

목적 : 자궁내막 증식증 및 자궁내막 선암에서 HGF, c-met의 발현 및 혈관신생과 임상 경과와의 연관성을 알아보기 위하여 연구를 시행하였다. 연구방법 : 자궁내막 증식증 19예, 자궁내막 선암 30예 등 총 49예의 환자에서 면역 조직화학적 염색을 이용하여 HGF, c-met의 발현 및 혈관신생을 조사하였다. HGF 및 c-met의 면역 조직화학적 염색은 각각의 특이항체를 이용하였으며, 혈관신생은 anti-CD31을 이용한 면역 조직화학적 염색으로 나타나는 혈관내피세포를 미세혈관으로 간주하여 현미경 하에서 그 수를 측정하였다. 결과 : 정상 자궁내막, 자궁내막 증식증 및 자궁내막 선암에서 HGF의 미만성 발현은 각각 45.5, 52.6 및 63.3 %로 나타나 유의한 차이를 보이지 않았으나, c-met의 미만성 발현은 각각 9.1, 36.8 및 60.0 %로 나타나 자궁내막 선암에서 미만성 발현의 빈도가 높았으며, 또 c-met의 미만성 발현은 진행된 병기 및 세포 분화도가 낮은 경우에서도 높은 빈도로 나타났다. 미세혈관의 수의 중앙값은 정상 자궁내막이나 자궁내막 증식증에서 보다 자궁내막 선암에서 현저히 증가됨을 보였으며 (5, 7 vs 22), 단순 자궁내막 증식증보다는 복합 자궁내막 증식증에서 증가됨을 보였다 (6 vs 9). 또 미세혈관 수는 자궁내막 선암에서 진행된 병기나 림프선의 전이와 관련하여 그 수가 증가됨을 보였다. 결론 : 이상의 결과로 c-met의 과발현이나 미세혈관 수의 측정을 이용한 혈관신생의 조사는 자궁내막 선암에서 예후를 평가할 수 있는 주요한 인자로 이용될 수 있을 것으로 생각되었다. Objective : This study was carried out to evaluate the clinicopathologic significance of hepatocyte growth factor (HGF) and c-met expression as well as tumor angiogenesis in endometrial hyperplasia and adenocarcinoma. Method : By means of immunohistochemical staining, HGF, c-met expression, and angiogenesis were investigated in total of 49 patients (19 endometrial hyperplasia, 30 endometrial adenocarcinoma). HGF and c-met were identified with specific corresponding antibodies. To evaluate angiogenesis, the microvessels were highlighted by staining their endothelial cells immunohistochemically for anti-CD31. Areas close to the deepest myometrial invasion or those with the highest grade of endometrial hyperplasia and the highest angiogenic intensity were selected. Three fields of 400 magnification were selected for each slide, and the mean microvessel count was obtained. Results : Diffuse staining for HGF was demonstrated in normal, endometrial hyperplasia and endometrial adenocarcinoma tissue in 45.5, 52.6 and 63.3 percent, respectively, while that for c-met was demonstrated in 9.1, 36.8 and 60.0 percent, respectively. c-Met overexpression was significantly correlated with high surgical stage as well as poor cellular differentiation. There were significant differences in microvessel count among normal, complex endometrial hyperplasia and adenocarcinoma (median 5, 9 vs. 22) and was between simple and complex endometrial hyperplasia (median 6 vs. 9). The microvessel counts were also significantly correlated with high surgical stage, poor cellular differentiation, lymph node involvement and deep myometrial invasion. Conclusion : This results suggested that both c-met overexpression and angiogenesis measured by microvessel count could be significantly important prognostic indicators for the prognosis of endometrial adenocarcinoma.

Paravesical Space에 발생한 혈관근섬유모세포종

정재혁 ( Jeong Jae Hyeog ),신병섭 ( Shin Beong Sup ),윤만수 ( Yoon Man Soo ),김지연 ( Kim Ji Yeon ),김기형 ( Kim Gi Hyeong ) 대한산부인과학회 2003 Obstetrics & Gynecology Science Vol.46 No.7

Angiomyofibroblastoma is a benign, distinctive, and rare soft tissue tumor of vulva and vagina that might be mistaken for aggressive angiomyxoma. It is suggested that angiomyofibroblastoma is slow-growing in nature and consistent with the benign clinical course reported in the literatures. 21-year-old women complained of lower abdominal pain and odorous vaginal discharge. She had a large paravaginal mass obliterating vaginal canal. It was maybe absent 3 months ago, but it was occupied in paravesical space later. It revealed angiomyofibroblastoma, which rapid grew and located in vagina. It was removed by vaginal approach. This report describes a case of angiomyofibroblastoma in paravaginal space characterized by rapid growth.

초경전 여아의 연소형 난소과립막세포종 1 예

박종훈 ( Jong Hoon Park ),김기형 ( Ki Hyung Kim ),이동형 ( Dong Hyung Lee ),윤만수 ( Man Soo Yoon ),안기헌 ( Ki Heon Ahn ) 대한산부인과학회 2001 Obstetrics & Gynecology Science Vol.44 No.7

Granulosa cell tumors are rare in children, and less than 5% of all cases occur before puberty. In premenarcheal girls, the juvenile type of this tumor usually elicits the signs of sexual precocity. We have recently experienced a case of juvenile granulosa cell tumor of the ovary presented with sexual precocity. It is different from the adult granulosa cell tumor with regard to clinical and pathological features as well as biological behaviour as previously described by Scully. Only the clinical stage at the time of diagnosis is considered to be related to the prognosis. About 90% are diagnosed in early stage with a favorable prognosis. More advanced stage have a poor clinical outcome.