Nitric oxide 의 자궁내막 세포 증식 억제효과에 관한 연구

김기형(Ki Hyung Kim),나용진(Yong Jin Na),이규섭(Kyu Sup Lee),김원희(Won Whe Kim) 대한산부인과학회 2001 Obstetrics & Gynecology Science Vol.44 No.8

N/A Objective : Development of endometriosis is associated with endometrial stromal cell proliferation. The effects of nitric oxide (NO) on the proliferation of endometrial cells and the effects of peritoneal fluid from women with and without endometriosis on the production of NO and NO-induced changes were investigated. Methods : Endometrial stromal cells were prepared from women with endometriosis (n=10) and healthy control (n=10). Peritoneal fluid from 10 patients were collected at the beginning of laparoscopic procedures. The proliferation of cells was assessed by [3H]thymidine. Nitrite, a stable NO product, was measured with Griess reagents. Expression level of endothelial nitric oxide synthase (eNOS) was analyzed by quantitative RT-PCR. Results : Sodium nitroprusside, an NO donor, reduced proliferation of the endometrial and endometriosis cells from primary culture in a dose-dependent manner. The increased production of NO and inhibitory effect of NO on the endometrial stromal cell proliferation were reduced by peritoneal fluid from patients with endometriosis but not by interleukin-8, 17-β estradiol or TGF-β1. Transcripts of the endothelial NOS (eNOS) gene were detected in unstimulated endometrial cells and the expression patterns were not changed by exposure of cells to SNP or peritoneal fluid for 24 hours. Conclusion : It is postulated that these findings show NO inhibits proliferation of endometrial cells and the inhibitory effect of NO on endometrial cells was abrogated by certain factor (s) of peritoneal fluid from patients with endometriosis. In conclusion, NO appears to be involved in the pathogenesis of endometriosis.

월반성 무월경에 대한 임상적 및 세포유전학적 고찰

최욱환(Ook Hwan Choi),이규섭(Kyu Sup Lee),윤만수(Man Soo Yoon),김원희(Won Whe Kim) 대한산부인과학회 1998 Obstetrics & Gynecology Science Vol.41 No.11

N/A The purpose of this study was to determine the frequency of chromosomal or genetic causes of primary amenorrhea, and was made to assess the etiology of disorders in those patients whose chromosome appeared normal. Sixty eight patients with primary amenorrhea were evaluated clinically and cytogenetically, which were refered to our Cytogenetic Laboratory in Department of Obstetrics and Gynecology, Pusan National University Hospital, from Aug. 1988 to Dec. 1996. The results were as follows. l. Out of 68 cases with primary amenorrhea, 40 cases (58.9%) had the normal chromosome constitutions and 28 cases (41.1%) had the abnormal chromosome constitutions including 46, XY. 2. Turner's syndrome was found in 25 cases (36.7%), consisting of 11 cases (16.1%) of 45,X, 3 cases (4.3%) of 46,X,i (Xq), 1 case (1.5%) of 46,X, inv (X), 1 case (1.5%) of 46,X, del (Xq), 1 case (1.5%) of 46,X, del (Xp), 1 case (1.5%) of 46,X, tel (Xq), 1 case (1.5%) of 45,X/46,XX, 1 case (1.5%) of 45,X/46,XY, 1 case (1.5%) of 45, X/47,XXX, 2 cases (2.9%) of 45,X/46,X, del (Xq), I case (1.5%) of 45,X/46,X, del (Xq), 1 case (1.5%) of 45,X/46,X, r (X). 3. 3 cases (4.3%) had the 46,XY chromosome constitution consisting of 2 cases (2.9%) of testicular feminization syndrome and 1 case (1.5%) of pure gonadal dysgenesis. 4. Among 40 patients whose chromosome are normal, the etiologies of amenorrhea were assumed to be caused by 11 cases (27.5%) of hypogonadotropic hypogonadism (idiopathic), 10 cases (25.0%) of congenital absence of vagina, 5 cases (12.5%) of pure gonadal dysgenesis in order of frequency.

자궁경부 원추절제술 후 시행한 자궁경관내 세포진 검사의 역할에 관한 연구

윤만수(Man Soo Yoon),이규섭(Kyu Sup Lee),최욱환(Ook Hwan Choi),김원희(Won Whe Kim) 대한산부인과학회 2000 Obstetrics & Gynecology Science Vol.43 No.6

목적 : 자궁경부 원추절제술 후 잔여병변을 예측하는 방법으로서 자궁경관 세포진 검사의 효용성을 알아보고자 연구를 시행하였다. 방법 : 자궁경부의 고도의 상피내 종양이나 미세침윤암으로 원추절제술 시행 후 자궁적출술을 시행한 229명의 환자를 대상으로 원추절제된 조직의 절단경계면의 상태, 원추절제술 후 시행한 자궁경관 세포진 검사의 결과와 자궁적출술 후의 조직에서의 잔여병변과의 연관성을 조사하였다. 결과 : 원추절제술 후 절단경계면의 상태와 잔여병변의 관계에서 절단경계면에 병변의 침범이 있었던 경우 52.6 % (41/78)에서 자궁적출술 후 잔여병변이 발견되었으나 절단경계면에 병변의 침범이 없었던 경우 8.6 % (13/151)에서만이 잔여병변이 발견되었다. 원추절제술 직후 시행한 자궁경관 세포진 검사 결과에 따라서는 세포진 검사가 양성인 경우 76.5 % (26/34)에서 잔여병변이 발견된 반면 세포진 검사가 음성인 경우는 14.4 % (28/195)에서만이 잔여병변이 발견되었다. 결론 : 원추절제술 후 잔여병변의 예측을 위해 자궁경관의 상태를 파악하는데 있어 자궁경관 소파술 대신 자궁경관의 세포진 검사를 시행하는 경우도 잔여병변의 예측에 효율적인 방법이 될 수 있을 것으로 사료된다. Objective : To evaluate the impact of the cytologic smear of the endocervical canal immediately after cervical conization on the rate of residual cervical lesions. Method : A retrospective review of 229 patients who underwent cervical conization for high grade cervical intraepithelial neoplasia or microinvasive cancer followed by hysterectomy. Endocervical Papanicolaou smear with a cytologic brush performed immediately after cervical conization in all 229 patients. Results : Fifty-four of 229 patients undergoing cervical conization followed by hysterectomy had residual lesion in the hysterectomy specimen. The prevalence rate of residual lesion according to the margin status of conization specimen as well as the results of the postconization Papanicolaou smear of the endocervical canal was compared. Forty-one of 78 patients (52.6 %) with positive margins had residual lesion compared with thirteen of 151 patients (8.6 %) with negative margins. Twenty-six of 34 patients (76.5 %) with positive postcone cytology had residual lesion compared with twenty-eight of 195 patients (14.4 %) with negative postcone cytology.Conclusion : Postcone cytologic smear of the endocervical canal as well as margin status are useful in predicting residual lesion after conization.

가토 질측원개점막에 대한 각종 자극이 소화장기에 미치는 영향에 관한 병리조직학적 연구

김원희 부산의대 1976 부산의대잡지 - 부산대학교 의과대학 Vol.16 No.17(증간호)

원발성 월경통 환자에 대한 Piroxicam의 임상효과

김원희 최신의학사 1984 最新醫學 Vol.27 No.6

가토에 있어서 거세후 질성숙지수의 변화에 관한 연구

김원희,김동휘 최신의학사 1989 最新醫學 Vol.32 No.3

급성 골반염 환자에 있어서 Unasyn^(�) (Sulbactam/Ampicillin)의 임상적 효과

김원희,신반철,이이경 최신의학사 1988 最新醫學 Vol.31 No.4

골아세포의 주사전자현미경적 소견에 관한 연구 : 에스트로젠과 프로제스테론의 영향을 중심으로

이규섭,최욱환,윤만수,김원희 대한폐경학회 1997 대한폐경학회지 Vol.3 No.2

The exact mechanism of bone loss in postmenopausal women is not known, but both increased bone resorption and decreased bone formation have been responsible, Theoretically, these effects may result from either increased activity of osteoclasts or decreased activity of osteoblasts, or both. In vitro, various kinds of substances administered either leads to activation or suppression of osteoblasts. The effects of steroid hormones such as estradiol and progesterone on osteoblast differentiation also is not well understood, but known to be helpful to maintain bone density. We studied the effects of l7 B-estradiol and progesterone on the differentiation of osteoblast- like cells by observing their morphologic characteristics by means of scanning electronmicroscopy (SEM). Human osteoblast(RCB0454) supplied by Riken Cell Bank of Tsukuba, Japan were grown in DME media with 10% fetal bovine serum(Gibco, U.S.A,), 10nM dexamethasone and penicillin-streptomycin for 6 days. After then, the cells were treated with dexamethasone 10nM with either 17 B -estradiol 10nM and 100nM or progesterone 1nM and 10nM. For control study, only alcohol vehicle given for 2 days. Scanning electron microscopy showed that estradiol and progesterone, though the latter showed less remarkable changes, trigger a production and release of matrix vesicle-like material that means to form procollagen and collagen where calcium and other minerals deposit to form bone and other cellular differentiation including ultimate necrosis to become flat and polygonal in shape compared to those of control cells. These findings indicate that both estradiol and progesterone stimulates matrix development and induces an induction of primary step of mineralization in human osteoblast(RCB0454) cell line. These findings suggested these steroid hormones stimulate to form new bone

골아세포내 소포의 미세구조에 관한 연구

이규섭,김기형,황인섭,원석,김원희,고형권,김경리 대한폐경학회 1998 대한폐경학회지 Vol.4 No.1

"The exact cause of bone loss in menopausal women is not known, but both increased bone resorption and decreased bone formation have been responsible. Theoretically, these effects may result from either increased activity of osteoclasts or decreased activity of osteoblasts, or both. In vitro, various kinds of substances administered either leads to activation or suppressicn of osteoblasts. We studied the ultramicroscopic structure of osteoblast-like cells by means of transmission electron microscopic examination. RCB cells (osteoblast from Riken Cell Bank) were grown in DMEM media with 10nM dexamethasone for 6 days, After then the cells were treated with dexamethasone. Matrix vesicles have been well documented as a unique type of extracellular structure. And these vesicles are the initial site of the mineral deposition. Akisaka et al. has classified these matrix vesicles into three categories, eg, granular type, flocculent type and amorphous type while Yoshida et al. did into four types eg. empty, amorphous, crystalline and ruptured type. These vesicles, already with calcium and other minerals, produced and released from osteoblast form procollagen and collagen and other cellular differentiation including ultimate necrosis compared to those of control cells. Author observed vesicles present in the cells by means of TEM as not much observations could be found in the literature as the matrix vesicles. Interestingly enough, vesicles both in and outside the osteoblasts showed some differences. Rather light and homogeneous vesicles which might be the source of empty type of matrix vesicles were in young and less necrotic cells. Second types were those with granular type in which diffuse granules seen without any shape. There were also vesicles with homogeneous dark color as well as with shapes of stripes, lamination or needle-like crystals. These findings suggests the vesicles that is the precursors of the matrix vesicles in the osteoblast shows different pattern according to the density of electron microscopic illumination depending of the mineral content and shapes. "

폐경기 여성에 대한 호르몬 대체요법후 혈청지질 분포의 변화 : Tibolone 과 Estradiol Cypionate 의 비교를 중심으로 Tibolone vs. Estradiol Cypionate

이규섭,김기형,황인섭,이후형,최욱환,김원희,나용진 대한폐경학회 1997 대한폐경학회지 Vol.3 No.1

This study was designed to establish serum lipid profiles in postmenopausal women after estrogen replacement therapy and identify the relationship with age, body weight, height and duration of treatment. Sixty-seven postmenopausal women were assessed. The serum total cholesterol, HDL cholesterol, LDL cholesterol and triglyceride distribution according to age, body weight, height and treatment duration were studied. Estradiol cypionate was given to 35 women and tibolone was given to 32 women. Blood samples were obtained at 12 months, 24 months and 36 months after hormone replacement therapy. HDL cholesterol was negatively correlated to age, but other lipid profiles were not correlated to age. Height and weight did not affect lipid profiles. Beneficial effect of menopausal estrogen was showed in both groups. LDL cholesterol decreased according to treatment duration in both groups. HDL cholesterol increased in E group, but decreased initialiy in tibolone group but returned toward baseline slowly over years.