일시적 대뇌허혈에 의한 gerbil 해마의 피라밋층에 조직학적 변화

양제훈,고필옥,곽수동,Yang, Je-hoon,Koh, Phil-ok,Kwak, Soo-dong 대한수의학회 2001 大韓獸醫學會誌 Vol.41 No.4

Cardiac arrest, hypoxia, shock or seizure has been known to induce cerebral ischemia. This study was designed to investigate the effect of ischemia on hippocampal pyramidal layer induced by transient bilateral occlusion of the common carotid arteries. Mature Mongolian gerbils were sacrificed at days 2, 4, and 7 after carotid occlusion for 10 minutes. Sham-operated gerbils of control group were subjected to the same protocol except for carotid occlusion. During operation for ischemia, body temperature was maintained $37{\pm}0.5^{\circ}C$ in all gerbils. Paraffin-embedded brain tissue blocks were cut into coronal slices and stained with H-E stain or immunostain by TUNEL method. Neurons with the oval and prominent nucleus and without the eosinophilic cytoplasm in the subfield of hippocamapal pyramidal layer were calculated as to be viable neurons. Their chromatins were condensed or clumped. Their nuclei appeared multiangular or irregularly shrinked. The width of the pyramidal layer was reduced due to the loss of nuclei. At day 2 after reperfusion, some neurons in the CA1 subfield were slightly eosinophilic. But most neurons in the CA2 subfield were strongly eosinophilic. At day 4 day, most neurons in the CA1 subfield were severely damaged and at day 7 day, only a few survived neurons were observed. Survived neurons per longitudinal 1mm sector in the CA1, CA2, CA3, and CA4 subfields of pyramidal layer were investigated. At day 2, the mean numbers of pyramidal neurons in CA1, CA2, CA3, and CA4 subfiedls were 104.5/mm (54.3%), 51.0/mm (33.8%), 105.5/mm (85.6%), and 124.3/mm (93.5%) compared to the nonischemic control group, respectively. At day 4, the mean numbers of pyramidal neurons in CA1, CA2, CA3, and CA4 subfields were 3.2/mm (1.7%), 51.5/mm(34.2%), 95.3/mm (77.4%), and 112.5/mm (84.6%), respectively. At day 7, the mean numbers of pyramidal neurons in CA1, CA2, CA3, and CA4 subfiedls were 0.8/mm (0.4%), 5.7/mm(3.8%), 9.8/mm (8.0%), and 5.0/mm (3.7%), respectively. The mean numbers of apoptotic positive neurons in the CA1 subfield at day 2, 4, and 7 after reperfusion were 67.8/mm, 153.2/mm and 123.7/mm, respectively. These results suggest that the transient cerebral ischemia cause severe damages in most neurons at day 7 and that the prosminent apoptotic positive neurons in hippocampal pyramidal layer are the delayed neuronal death induced by ischemia.

Evaluation of Antiangiogenic Effects of a New Synthetic Candidate Drug KR-31831 on Xenografted Ovarian Carcinoma Using Dynamic Contrast Enhanced MRI

양제훈,김재훈,임근호,허혜정,윤세라,이재원,이정희,전평 대한영상의학회 2011 Korean Journal of Radiology Vol.12 No.5

Objective: The purpose of this research was to investigate the anti-angiogenic inhibitory effect of KR-31831, a newly developed anti-angiogenic agent, on an in vivo human ovarian carcinoma model using dynamic contrast-enhanced (DCE) MRI. Materials and Methods: Xenografted ovarian tumors were established by subcutaneous injection of SKOV3 cells into mice. The mice were treated daily with KR-31831 at 50 mg/kg for 21 days. Tumor tissues were excised corresponding to the DCE-MRI sections for evaluation of MVD with CD31 immunohistochemistry. All in vivo MRIs were performed on a 7.0 Tesla micro-MRI System. DCE-MRI was acquired prior to initiating treatment with KR-31831 and again on days 3 and 21 after treatment. The permeability parameters (Ktrans, ve, and vp) were estimated using a pharmacokinetic model. Results: Qualitatively, the Ktrans parametric mapping showed different changes before and after treatment with KR-31831 in the treatment group. For quantification of this change, the median of Ktrans values were compared before and after treatments in the control and KR-31831-treated groups. A non-parametric statistical test (Wilcoxon signed-rank test) showed decreasing Ktrans values on day 21 compared to days 0 and 3 in the KR-31831-treated group (p < 0.05), whereas there was no significant difference in the control group (p = 0.84). Conclusion: Our results suggest that DCE-MRI can be a useful tool by which to evaluate the anti-angiogenic effect of KR-31831 on a xenografted human ovarian carcinoma model.

The Effects of Cyclophosphamide on Apoptosis in Murine Lymphoma

Yang, Jehoon,Bae, Hyung Joon,Seo, Deuk Rok,Koh, Phil Ok,Kwak, Soo Dong 대한의생명과학회 2001 Biomedical Science Letters Vol.7 No.4

Whereas apoptosis is a critical mode of cell deletion in normal organism development, apoptotic cells are also obserced in tumor therapy. We therefore investigated the expression of apoptotic cells induced as a function of time and dose in murine A-20 lymphoma treated with cyclophosphamide in vivo, by H&E and TUNEL method. The percent of apoptotic cells were scored from tumor section using TUNEL method. The expression of apoptotic positive cell was determined over a 10-day period following treatment of the mice with 200 mg/kg. Apotosis increased further with time, reaching a peak value between 12∼24 hr (scored 6.7±1.0%∼6.1±0.7%), and then slowly declined to backgroud levels by 10 days after treatment. The dependence of induction of apoptosis on the dose of cyclophoshamide was determined by treatment with 50, 100, or 200 mg/kg at 12 hr after treatment. Apoptosis was dose dependent in that as the dose was increased the percentage of apoptosis increased. However, the increase in apoptosis at the lower dose used (50 mg/kg was higher on a per unit dose basis than that at the higher dose used (200 mg/kg). This result show that the alkylating agent cyclophoshamide strongly induces apoptosis in murine lymphoma.

당뇨병성 BBDP Rat에 있어서 난포내 난자의 핵상과 성숙

전용필,양제훈,김정훈,강병문,장윤석,목정은,김길수 한국실험동물학회 1998 한국실험동물학회 학술발표대회 논문집 Vol.1998 No.-

랫드의 개방형 경골골절 모델의 개발

박규상,양제훈,한진수,김길수 한국실험동물학회 1999 한국실험동물학회 학술발표대회 논문집 Vol.1999 No.-

임신우에서 발생된 난포의 기능에 대한 면역조직화학적 관찰

곽수동,고필옥,양제훈,원청길,강정부,Kwak, Soo-Dong,Koh, Phil-Ok,Yang, Je-Hoon,Won, Chung-Kil,Kang, Chung-Boo 대한수의학회 2003 大韓獸醫學會誌 Vol.43 No.4

Incidence of estrum or abortions in pregnant cows may be affected by large follicles developed together with corpus luteum in pair ovaries of pregnant cows. But the follicles of pregnant phase were not assessed about histological findings. Determination of the healthy and atretic follicles by presence of proliferative cells or apoptotic cells and histological compositions of follicles would be used as important data on measurements of ovarian functions. This study was focussed mainly to investigate macroscopical, histological and immunohistochemical findings of ovarian follicles of pregnant Korean native cows and dairy cows (Holstein). In immunohistochemical methods, assessments of proliferative cells using PCNA antibody and apoptotic cells using TUNEL methods were performed. The follicles were observed on all 24 pregnant cows (17 Korean native cows and 7 Holstein cows). Follicles of greater than 10 mm in daimeter were developed in 37.5% (9/24 heads) of these pregnant cows. largest follicles from in these cows were $16.0{\times}15.0mm$ in diameter in a Korean native cow(l20 days of gestation), $13.4{\times}10.1mm$ in a Korean native cow(50 days of gestation), $12.9{\times}11.5mm$ in a Holstein cow (120 days of gestation). 40.5% among all follicles having diameter of greater than 1.0 mm in pregnant cows were assessed as atretic follicles and in addition, healthy follicles also showed less in number and smaller in size and thinner in wall layer compared with those of cyclic phase ovaries. In immunohistochemical findings, also proliferative positive cells and apoptotic positive cells on the granulosa cell layers in the healthy follicles of pregnant cows appeared less than on those of cyclic follicles. So these follicles were assessed as weakly active follicles. In large follicles, above positive cells were not nearly appeared but granulosa cell debris were more appeared among the granulosa cells. So these large follicles were assessed as inactvie or atretic follicles. The above findings suggest that small follicles of pregnant phase were weakly active or atretic and large follicles were inactive or atretic.

Spontaneously Hypertensive Rat(SHR)와 BBDP/Wor rat의 교차교배를 통한 교잡종의 특징

김길수,정영길,양제훈 한국실험동물학회 1998 한국실험동물학회 학술발표대회 논문집 Vol.1998 No.-

한우의 임신경과에 따른 황체조직의 광학 및 전자현미경적 변화

표병민,고필옥,양제훈,원청길,조규완,강정부,곽수동,Pyo, Byong-min,Koh, Phil-ok,Yang, Je-hoon,Won, Chung-kil,Cho, Gyu-wan,Kang, Chung-boo,Kwak, Soo-dong 대한수의학회 2003 大韓獸醫學會誌 Vol.43 No.3

Corpus luteum (CL) is the primary productive organ of progesterone in pregnant cows. Progesterone levels in bovine plasma depend on the volume, weight and shape of the CL. Progesterone productions during the late stages of gestation occur both in the CL and placenta, and placentas producted more progesterone than CL on progesterone prcduction. Because division of progesterone production of these two organs is impoxxible, the CL function can not be determined by plasma progesterone levels following gestation stages. This study was carried out to evaluate histological findings on the CL spurium and CL verum, and also on the CL following the pregnant stages by histological and immunohistochemical and electron microscopical methods and then we expect to assume the functions of CL by histological findings. 1. Proliferations of luteal cells occur by day 120 of gestation, vessel hyperplasia occur by day 90 of gestation, and the walls and lumens of vessels developed by day 120 of pregnancy. 2. Sizes of CL cells increased to maximum around day 200 of gestation and similarly maintained by day 240. So these findings indicated that the function of Cl is most active around day 200 of gestation. 3. On parturation day, the number and size of luteal cells were maintained but stain intensity of the luteal cells and vessels are declined or disappeared, and fibrosis of luteal cells increased, and the vessel lumens are emptied. These findings indicate that CL is inactive. 4. In immunohistochemical findings, proliferative positive cells by PCNA antibody appeared more in number during early stages of gestation but appeared less following course of pregnant stages and not nearly appeared on day 120 of gestation. Apoptotic positive cells by TUNEL methods not nearly appeared on the early pregnant stages and a few appeared at late pregnant stages. So developments of CL proceed until day 120 of gestation and regression of CL was occurred by transform of luteal cells into fibrocytes than by luteal cell apoptosis. 5. In electron microscopical findings, the size of luteal cells increased more in CL verum than in CL spurium. During gestation stages, the size of luteal cells increased, mitochondria in the luteal cell cytoplasms densely and abundantly developed and also swelled mitochondria increased. The interspace of luteal cells are also dilated, transformation of luteal cells into fibrocytes are more number. The lumens and walls of peripheral capillaries of large luteal cells more broadened and thickened, and transformation of large and small luteal cells to fibrocytes are increased. The above findings suggest that function of pregnant CL more developed by day 120 of gestation and are most active around day 200 of gestation and similarly maintained by day 240 and are promptly regressed on paturation day.

Active immunization using dendritic cells mixed with tumor cells inhibits the growth of Lymphoma

박진희,김상희,서철원,양제훈,박정선,조화정,김효정,김태원,이제환,김성배,김상위,이규형,이정신,김우건 대한내과학회 1999 대한내과학회 추계학술대회 Vol.57 No.-

말의 흉추골유합과 요추골변형의 1례

김종섭,송치원,조규현,이상래,양제훈,원청길,Kim, Chong-Sup,Song, Chi-Won,Cho, Gyu-Hyen,Lee, Sang-Rae,Yang, Je-Hoon,Won, Chung-Kil 대한수의학회 2003 大韓獸醫學會誌 Vol.43 No.1

The fused thoracic vertebra and deformity of the lumbar vertebrae in the female Thoroughbred horse were observed macroscopically. The 11th and 12th thoracic vertebrae (T) were partially fused. They were composed of three parts between the spinous process of the 11th and 12th T, left and right caudal articular processes of the 11th T, and left and right cranial articular processes of the 12th T. The vertical surface of cranial articular process of the 11th T and left mamillary process of the 12th T were absent. The left caudal part of the transverse process of the 12th T and left costal fovea of the transverse process of the 12th T were severely deformed. On the other hand, the left transverse process of the first lumbar vertebra (L) was a typical rib-like transverse process and two times longer than the right transverse process of the 2nd L. The right transverse process of 4th L has an oval concave facet on the medial part of caudal border for articulation with the right transverse process of 5th L; the latter has a corresponding convex facet on the medial part of the cranial border in the right transverse process of the 5th L.