Hymenochirus boettgeri의 궁둥신경 절단이 뒷다리뼈 발육에 미치는 영향에 대한 연구

손장신(Jang-Shin Sohn),김형태(Hyoung-Tae Kim) 대한체질인류학회 2007 해부·생물인류학 (Anat Biol Anthropol) Vol.20 No.4

본 연구는 Hymenochirus boettgeri의 궁둥신경 절단이 뒷다리 뼈 성장에 어떤 영향을 주는지 알아보고자 시도하여 다음과 같은 결과를 얻었다. 실험동물은 각각 Stage 63과 66 때 궁둥신경을 절단하여, 각각 6과 7주 후, 그리고 12와 13주 후에 alcian blue와 alizarine red로 염색하여 영상분석기로 넙다리뼈의 길이와 평면적, 정강뼈의 길이를 측정하였다. 첫째로, 단순한 피부절개는 뼈의 성장이나 발육에 영향을 주지 않으며, 둘째로 궁둥신경 절단은 넙다리뼈와 정강뼈의 길이성장에 영향을 주어 2~9%의 감소를 보여주었고, 셋째로, 궁둥신경 절단은 넙다리뼈의 단위평면적에 도 영향을 주었다. 넷째로, 신경절단된 넙다리뼈의 골화과정의 지연이 형태학적으로 관찰되었다. 이상의 결과는 다른 연구자들의 연구와 종합해 볼 때 궁둥신경의 절단은 넙다리뼈와 정강뼈의 성장에 직접적인 영향을 준다고 사료된다. 또한 이러한 다리뼈의 성장의 감소는 골화과정의 지연과 뼈성장과정의 임계시기의 상실에 의한 것이라 사료된다. The effect of peripheral nerve on limb growth and maturation has received little attention after the limb differentiation stage. This study investigated the effects of paralysis (loss of function) on growth of bones in the hind limb of Hymenochirus boettgeri. Complete denervation of the right sciatic nerve was performed at stages 63 and 66, animals were sacrificed after 6 and 7 (Groups I and Ⅱ) and 12 and 13 (Groups Ⅲ and Ⅳ) weeks. Denervation was assessed by degree of paralysis. Specimens were cleared and double stained with alcian blue and alizarin red for cartilage and bone. Length and area of right and left femurs and length of right and left tibia were measured by using an image analyzer program after photographing, and the ratio of right to left femur length and area was calculated. There were no gross morphological differences between the control and sham groups. In the experimental groups, the ratio of femur length was 93.71% and 95.70% in Group Ⅰ and Ⅱ, and 96.12% and 96.06% in Group Ⅲ and Ⅳ. The ratio of tibia length was 94.05% and 96.15% in Group Ⅰ and Ⅱ, and 98.12% and 98.22% in Group Ⅲ and Ⅳ. The ratio of femur area was 90.43% and 95.61% in Group Ⅰ and Ⅱ, and 96.08% and 95.73% in Group Ⅲ and Ⅳ. Comparison between control and experimental groups showed statistically significant (p<0.05). There was a histologically maturational delay in proximal end of denervated hind limb, comparing with opposite side. These results suggested that denervation of sciatic nerve affect directly the growth and maturation of hind limb bones in Hymenochirus boettgeri and loss of critical period of bony maturation after denervation.

부동스트레스에 의한 심방근육세포막 투과성 변화의 형태학적 관찰

신해영(Hae Young Shin),송창호(Chang Ho Song),한인혁(Eui-Hyeog Han),손장신(Jang Sihn Sohn),박춘수(Chun Soo Park),이무삼(Moo Sam Lee) 대한해부학회 2002 Anatomy & Cell Biology Vol.35 No.4

부동스트레스가 심방근육세포의 막투과성에 미치는 영향을 알아보기 위하여, 흰쥐를 6시간, 12시간, 24시간동안 플라스 틱관에 넣어 스트레스를 준 후 면역조직화학적 방법으로 심방근육세포내 myoglobin과 α-actinin의 변화를 관찰하고, 전자 현미경으로 lanthanum 추적법을 이용하여 심방근육세포의 막투과성을 관찰하였다. 심방근육세포내 myoglobin과 α-actinin의 변화 부동스트레스 6시간군과 12시간군에서는 심방근육세포내 myoglobin과 α-actinin의 양성반응이 대조군에 비하여 약하였다. 부동스트레스 24시간군에서는 myoglobin과 α-actinin 반응이 12시간군에 비하여 부분적으로 증가하는 경향이었으나 대조군 수준보다는 약하였다. Lanthanum (La) 추적법에 의한 전자현미경 관찰대조군에서 La는 심방근육세포의 세포사이공간에만 침착되었다. 부동스트레스 6시간군에서는 심방근육세포의 미세구조적 변화를 보였으며, La는 심방근육세포내 근육원섬유와 사립체 주위, 사립체 바탕질에 침착되었다. 부동스트레스 12시간군과 24시간군에서도 약간의 심방근육세포의 형태학적 변화를 보였으며, La는 부동스트레스 12시간군에서 심방근육세포의 근육원섬유와 사립체 주위, 사립체 바탕질에 침착되었으나, 부동스트레스 24시간군에서는 주로 심방근육세포의 세포사이공간에 침착되었고, 드물게 근육형질에서도 관찰되었다. 위의 결과들은 부동스트레스가 심방근육세포의 막투과성과 미세구조에 영향을 미친다는 것을 보여준다. This study was performed to investigate the effect of immobilization stress on the ultrastructural changes and membrane permeability in rat atrial myocyte using immunohistochemical and lanthanum tracer techniques. Male Sprague-Dawley rats, body weight 160~200 g, were used for all immobilization stress group. Rats were immobilized in small round plastic tube for 6, 12 or 24 hours, except for the control group. Alterations of myocardial myoglobin and α-actinin as well as membrane permeability after immobilization stress were examined by immunohistochemistry, and lanthanum permeability of the rat atrial myocyte were observed by electron microscopy. In the control group, there was no loss of myoglobin or α-actinin from the atrial myocytes. After 6 and 12 hours immobilization stress, the loss of myoglobin and α-actinin could be identified the atrial myocytes. In the 24 hour immobilization groups, the content of the myoglobin and α-actinin recovered partially. Lanthanum was deposited only in the intercellular space of the atrial myocardium in the control group. In the 6 hour immobilization group, the atrial myocytes showed severe ultrastructural changes during immobilization stress. Lanthanum deposited in the sarcoplasm, myofibrils, adjacent of mitochondria, and mitochondrial matrix. In the 12 or 24 hour immobilization groups, the morphological alteration of atrial myocytes appeared weekly. In the 12 hour group, lanthanum deposited in myofibrils, adjacent of mitochondria and in the mitochondrial matrix. In the 24 hour group, lanthanum deposited mainly in intercellular space of atrial myocardium, and rarely in the sarcoplasm of myocytes. These results suggest that the immobilization stress may induce the alteration of cardiac cell membrane permeability and the ultrastructures of atrial myocardium.

소세포 폐암의 유두부 전이에 의한 폐쇄성 황달

박홍배(Hong Bae Park),임연근(Yeun Keun Lim),여향순(Hyang Soon Yeo),장진형(Jin Hyeong jang),김연종(Yeon Jong Kim),김호동(Hou Dong Kim),윤형신(Heyong Shin Yoon),이윤호(Youn Ho Lee),손장신(Jang Shin Sohn) 대한소화기학회 1993 대한소화기학회지 Vol.25 No.6

A 56-year-old man was admitted to our hospital because of jaundice. His chest radiography revealed a mass lesion in the left upper lung. Bonchoscopic lung biopsy was performed and the pathological diagnosis was small cell carcinoma of the lung. In his clinical course, obstructive jaundice developed during combination chemotherapy. The findings of abdominal ultrasonography, duodenoscopy with biopsy and percutaneous transhepatic cholangiography suggested that jaundice was developed by extrahepatic biliary obstruction with ampulla of vater metastasis. We experienced one case of obstructive jaundice by ampullary metastasis of small cell carcinoma of the lung cancer in 56-year-old man.

유방암치료 후 흉골에 발생한 방사선속발성 골육종 1예

임현우 ( Hyun Woo Lim ),전현정 ( Hyun Jung Jun ),염윤식 ( Yoon Shick Yom ),윤대성 ( Dae Sung Youn ),정원규 ( Weon Kuu Chung ),손장신 ( Jang Shin Sohn ),조도연 ( Do Yeun Cho ) 대한내과학회 2010 대한내과학회지 Vol.78 No.4

방사선 조사 부위에 이차적으로 발생하는 골육종은 그 빈도는 매우 낮으나 원발성골육종에 비해 나쁜 예후를 가지는 것으로 되어 있다. 저자들은 유방암에 대한 수술시행 후 보조 항암화학요법과 보조 방사선요법을 시행받은 여성에서 방사선에 의한 이차성 골육종의 발생을 경험하였다. 53세 여자 환자는 방사선 조사 6년 후 전흉부의 동통을 동반한 종괴를 주소로 내원하였고, 조직 검사상 흉골의 골육종으로 진단되었다. 진단 시 전이 부위가 없었으나 두 달 후 간전이가 발견되는 매우 공격적인 양상을 보였다. Radiation-induced osteosarcoma is a very rare complication of radiation therapy, with a poor prognosis. We experienced a case of radiation-induced osteosarcoma of the sternum, in a patient who had had breast cancer treated with surgery, adjuvant chemotherapy, and adjuvant radiation therapy 6 years earlier. A 53-year-old woman complained of a painful anterior chest wall mass. The mass was diagnosed as osteosarcoma on biopsy. The radiation-induced osteosarcoma had an aggressive nature. (Korean J Med 78:512-517, 2010)

자궁내막 증식증 및 선암종에서의 p53단백의 발현

김윤신,이미숙,임성철,서재홍,손장신 대한병리학회 1997 Journal of Pathology and Translational Medicine Vol.31 No.7

갑상선 결절에서의 미세침흡인세포 검사의 진단적 가치

이상석,김종관,오필석,김순호,임중규,허진득,손장신 대한내분비학회 1991 Endocrinology and metabolism Vol.6 No.2

1,067 patients were studied to assess value of Fine Needle Aspiration cytology (FNA) for the diagnosis of thyroid nodules at Kwangju Christian Hospital during a 5 year period from 1986 to 1990. Of 1,067 patients satisfactory aspiration specimens were obtained in 948 patients(88.8%). Thyroidectomy was performed in 187 of 1,067 patients who had done FNA prior to the surgery and the diagnostic value of the procedure was assessed in 177 of 187 patients who had satisfactory aspiration smears. 620 patients who were treated by thyroidectomy without FNA prior to the surgery were also analysed and the results were compared with the group who had done FNA prior to the surgery. Of 948 patients who had satisfactory aspiration specimen, 536 were follicular proliferation, 210 were involutional change, 73 were thyroiditis, 53 were follicular neoplasm, 5 were H rthle cell tumor and 70 were carcinoma. Of 187 patients who underwent thyroidectomy with FNA, 55 patients (29.4%) had carcinoma. Of 620 patients who underwent thyroidectomy without FNA, 137 (22.1%) had carcinoma. The sensitivity, specificity and accuracy. We conclude from the study that FNA is an extremely valuable means of differential diagnosis of thyroid nodular disease. (J Kor Soc Endocrinol 6:150~156, 1991)

이차성 알도스테론증을 동반한 부신피질 선암 1예

김호동,김연종,임중규,허진득,윤형신,장진형,김훤,손장신 대한내분비학회 1992 Endocrinology and metabolism Vol.7 No.2

Adrenal cortical carcinoma is a rare tumor that affects all ages and sexes and has a dismal prognosis. Half are functioning and half are not. Functioning neoplasma of the adrenal cortex are clinically manifested by virilization, Cushing's syndrome, feminization, or hyperaldosteronism. A 22-year-old female was admitted to our hospital in April 1990, because of amenorrhea for 6 months, hirsuitism, acne and palpable mass in left upper abdomen for 3 months. Routine laboratory examination including blood chemistry gave no abnormalities. Abdoiminal CT scan demonstrated a large mass with central necrosis and calcification, about 10×15 cm size in left upper abdomen. Endocrine tests revealed; serum testosterone 7.5ng/dl, DHEA-sulfate above 1,000ng/dl, 24-hour urine free cortisol 152 ng/day, 17-KS 50.8 ng/day, 17-OHCS 9.9mg/day. Plasma aldosterone and plasma renin activity were elevated. We report a case of adrenocortical carcinoma with hypersecretion of glucocorticoid and androgen.She also had a secondary aldosteronsm and lung metastasis. She underwent surgical resection of the tumor and treated with mitotan (O.P'-DDD) postoperatively (J Kor Soc Endocrinol 7:160~164, 1992).