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A549 폐암세포주에 대한 蘇本 水抽出物의 세포고사 유도효과
문연자,남용재,이광규,최두호,이성원,안성훈,최민규,우원홍 대한동의생리학회,대한동의병리학회 2002 동의생리병리학회지 Vol.16 No.3
The Caesalpinia sappan is widely used in the traditional oriental herbal medicine for anti-inflammatory, antioxidant effects. The effects of water extract of C. sappan on the cell viability and induction of apoptosis were investigated in human lung cancer cell line A549. The water extract of C. sappan produced apoptotic cell death and DNA fragmentation and nucleus chromatin condensation in A549 cells. The enzyme activity of caspase-3 and protein level of actived caspase-3 were markedly increased in A549 cells treated with the water extract of C. sappan. In addition, the extract of C. sappan induced cleavage of Poly (ADP-ribose) polymerase (PARP), a known substrate for caspase-3, and dropped in cellular ATP levels. These results suggest that the extract of C. sappan exerts anticancer activity by induction of apoptosis via activation of caspase-3, cleavage of PARP protein, and depletion of cellular ATP levels in A549 cells.
건조방법에 따른 눈개승마의 영양성분 및 항산화효과 비교
김정연,남용재,이재준 한국지역사회생활과학회 2023 한국지역사회생활과학회지 Vol.34 No.3
The purpose of this study was to compare the effects of two drying methods (hot air drying [HAD] or vacuum freeze drying [FAD]) on the physicochemical components and antioxidative activities of Aruncus dioicus var. kamtschaticus powder. The crude fat content in the FAD was higher than that in the HAD. There were no significant differences in the proximate compositions, such as contents of moisture, crude ash, crude protein, and carbohydrates, depending on the drying methods. The total amino acid and essential amino acid contents were significantly higher in FAD than in HAD. The valine, phenylalanine, lysine, histidine, aspartic acid, asparagine, glutamic acid, arginine, ornithine, and γ-amino-n-butyric acid (GABA) contents in FAD were higher than in HAD, while the serine, phosphoserine, proline, glycine, alanine, tyrosine, and taurine contents in HAD were higher than in FAD. There were no significant differences in saturated fatty acid, monounsaturated fatty acid, and polyunsaturated fatty acid contents based on the drying methods. The most abundant fatty acid in the FAD was linolenic acid, and the linolenic acid content was approximately the same, regardless of the drying method used. The contents of malic acid and total organic acids in the FAD were higher than in the HAD, while the content of succinic acid was higher in the HAD than in the FAD. Vitamin C and E contents in HAD were higher than those in FAD. The total polyphenol and total flavonoid contents, the 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2-azino- bis-3-ethylbenzothiazoline-6-sulphonic acid (ABTS) radical scavenging activities of the FAD sample were higher than those of the HAD sample. These results indicate that vacuum freeze drying is a better drying method for preserving the nutritional components and antioxidative activities. These study results may provide the basic data for follow-up studies seeking a better understanding of the functional properties of the FAD powder.
율(栗)에 대(對)한 한의학적(韓醫學的) 문헌고찰(文獻考察)
이수진,김명동,남용재,Lee Soo-Jin,Kim Myung-Dong,Nam Young-Jae 대한예방한의학회 2000 대한예방한의학회지 Vol.4 No.1
This dissertation is try to figure out why chestnut belongs to kidney channel, from the viewpoint of five elements theory. After studying chestnut's property, flavor, channel tropism, main cure ability, prescriptions, shape, sweet, and prohibitions, I came to the following results. 1. Property of chestnut is warm and has no toxicity, so it is less related than kidney property. 2. Flavor of chestnut is salty and sweet, so it has some relation to kidney and spleen properties. 3. Channel tropism of chestnut enters mainly into kidney channel, and then spleen and stomach channels. 4. Chestnut controls kidney function of storing the essence of life, determining the condition of bone and marrow, conduction water metabolism, affecting reasoning activity, and controls activity of nine openings of body. It also has effects on functions of spleen, intestines and stomach. 5. Prescriptions including chestnut is similar to that of human brain, it is possible to reason out that chestnut has some relation to human brain. 7. As flavor of chestnut flower is similar to that of spermatic fluid, so it has som relation to kidney property. 8. As chestnut has property of blocking qi and it causes spleen, stomach and colon system to be confused, so it is suggested that persons with weakende spleen and stomach be not allowed to take in.
원소분석기와 연계된 동위원소질량분석기(EA-IRMS)를 이용한 탄소안정동위원소비 정확도,정밀도 연구
김보경 ( Bo Kyong Kim ),남용재 ( Yong Jae Nam ),이원석 ( Won Seok Lee ),한진석 ( Jin Seok Han ),황종연 ( Jong Yeon Hwang ) 한국환경분석학회 2012 환경분석과 독성보건 Vol.15 No.4
The reproducibility as the standard deviation or standard error of analytical data is used to estimate the performance ability of the isotope ratio mass spectrometer (IRMS). The standard deviation of □^13C value of a sample is not supposed to be the same on a wide range of sample weights. In order to determine the suitable sample amounts required for high precision and accuracy and the factors required for optimal equipment conditions, certified reference materials were repeatedly measured with various weight ranges using an elemental analyzerisotope ratio mass spectrometer (EA-IRMS). The measured □^13C values of the standards EMA-P1 and EMAP2 fell within the certified value ±2σ with the minimum carbon contents of 102.107 μg and 116.879 μg, respectively. In addition, for the two standards, the peak height ratios calculated by the CO_2(^12C^16O^16O) peak height of the sample to the working standard were 62.8% and 66.8%, respectively. The isotopic composition change was also investigated by the fractionation factor □applied and did not show a large difference in a wide range of sample weight for the two standards. We suggest that carbon isotope composition needs to be determined by considering several factors such as element contents, standard deviation of □^13C value, peak height ratio(or peak height) and fractionation factor □applied.
대장(大腸)과 관련(關聯)된 경혈(經穴)들의 신경해부학적(神經解剖學的) 연구(硏究)
강창수,이상룡,이창현,남용재,이광규,Kang, Chang-Soo,Lee, sang-ryoung,Lee, Chang-Hyun,Nam, Yong-Jae,Lee, Kwang-Gyu 대한침구의학회 2000 대한침구의학회지 Vol.17 No.2
The purpose of this morphological studies was to investigate the relation between the meridian, acupoints and viscera using neuroanatomical tracers. The common locations of the spinal ganglia, sympathetic chain ganglia, spinal cord and brain projecting to the large intestine meridian were observed following injection of transganglionic tracer, WGA-HRP and transsynaptic neurotropic virus, pseudorabies virus(PRV), Bartha strain(Ba) and PRV-Ba-Gal (Galactosidase)) into the the large intestine(cecum, colon and rectum), ST37 and LI4. After survival times of 96 hours following injection into the thirty rats with WGA-HRP, PRV-Ba and PRV-Ba-Gal. They were perfused, and their spinal ganglia, sympathetic chain ganglia, spinal cord and brain were frozen sectioned($30{\mu}m$). These sections were stained by HRP and X-gal histochemical and PRV immunohistochemical staining method, and observed with a light microscope. The results were as follows : 1. WGA-HRP labeled neurons innervating the large intestine were observed bilaterally within the T13-L4 sympathetic chain ganglia, and T9-11 spinal ganglia. WGA-HRP labeled neurons innervating ST37 were observed within the L3-5 sympathetic chain ganglia, and L2-4 spinal ganglia. WGA-HRP labeled neurons innervating LI4 were observed in the middle cervical ganglion and stellate ganglion, and C5-8 spinal ganglia. 2. In spinal cord, PRV-Ba labeled neurons projecting to the large intestine, ST37 and LI4 were found in thoracic, lumbar and sacral spinal segments. Densely labeled areas of each spinal cord segment were founded in lamina N, V, VII(intermediolateral nucleus), Ⅸ, X and dorsal nucleus. 3. In medulla oblongata, PRV-Ba and PRV-Ba-Gal labeled neurons projecting to the large intestine, ST37 and LI4 were commonly found in the A1 noradrenalin cells/C1 adrenalin cells/caudoventrolateral reticular nucleus, dorsal motor nucleus of vagus nerve, nucleus tractus solitarius, raphe obscurus nucleus, raphe pallidus nucleus, raphe magnus nucleus and gigantocellular nucleus. 4. In pons, PRV-Ba and PRV-Ba-Gal labeled neurons were commonly found in locus coeruleus, Kolliker-Fuse nucieus and A5 cell group. 5. In midbrain, PRV-Ba and PRV-Ba-Gal labeled neurons were commonly found in central gray matter. 6. In diencephalon, PRV-Ba and PRV-Ba-Gal labeled neurons were commonly found in paraventricular hypothalamic nucleus. These results suggest that PRV-Ba and PRV-Ba-Gal labeled common areas projecting to the large intestine may be correlated to that of the large intestine meridian, ST37 and LI4. Especially, These morphological results provide that interrelationship of meridian-acupoints -viscera may be related to the central autonomic pathways.
스마트폰 기반 버스 정보시스템의 버스 노선 추천 시스템 설계
임재걸(Jaegeol Yim),이계영(Gyeyoung Lee),남용재(Yongjae Nam) 한국컴퓨터정보학회 2014 한국컴퓨터정보학회 학술발표논문집 Vol.22 No.2
근래에는 대부분의 지방자치단체에서 버스정보시스템을 운영하여 버스 고객에게 다양한 정보를 제공한다. 예를 들어, 기다리는 버스의 현재 위치를 알려주는 서비스가 버스정보시스템이 제공하는 대표적인 서비스 중 하나이다. 버스정보시스템은 버스의 현재 위치를 감지하는 다양한 센서들, 센서 데이터를 수집하는 통신시스템, 수집한 데이터를 분석하여 정보를 추출하는 서버시스템 등으로 구성되며, 구축비용이 어마어마하다. 본 저자는 버스정보시스템의 구축비용을 획기적으로 절약하는 방법으로 스마트폰을 이용하는 방법을 이미 제안한 바 있다. 본 논문은 스마트폰 기반의 버스정보시스템의 서버의 구성요소 중 고객이 승차하기를 원하는 버스 노선을 알아내는 버스노선 추천 시스템을 설계한다.
임재걸 ( Jaegeol Yim ),박병훈 ( Byeong-hun Park ),남용재 ( Yong-jae Nam ),원정완 ( Jungwan One ) 한국정보처리학회 2013 한국정보처리학회 학술대회논문집 Vol.20 No.1
비디오 영상은 파일이 크기 때문에 다운로드가 완료된 다음에 재생을 시작하는 것이 아니라 수신이 되는 대로 재생이 되어야한다. 이와 같은 스트리밍 서비스는 주문형 비디오 서비스를 비롯한 다양한 상용시스템에 필수요소이다. 스트리밍 기능을 제공하는 여러 가지 서버 중, 본 논문에서는 다윈 스트링 서버를 설치하고 주문형비디오 서비스 시스템을 구축하는 방법을 소개한다.