F344 랫드를 이용한 이황화메틸의 아급성 흡입독성연구

김현영,이성배,한정희,정용현,김형진,신진영,신동호,김종춘,이용묵,Kim Hyeon-Yeong,Lee Sung-Bae,Han Jung-Hee,Chung Yong-Hyun,Kim Hyoung-Chin,Shin Jin-Young,Shin Dong-Ho,Kim Jong-Choon,Lee Young-Mook 한국생명과학회 2005 생명과학회지 Vol.15 No.1

이황화메틸의 반복 흡입노출에 의한 아급성 독성 잠재력을 평가하기 위해 암수 랫드에게 0, 5, 25 및 125 ppm용량으로 21일간 반복 흡입노출하고, 일반증상과 체중, 사료섭취 량, 혈액치, 혈청생화학치 및 부검소견을 관찰하였다. 시험결과, 랫드에게 이황화메틸을 3주간 반복 흡입노출하면 125 ppm의 농도에서 체중증가의 억제와 사료섭취 량의 감소를 유발하나, 혈액 및 혈청생화학치에는 어떠한 이상도 유발하지 않는 것으로 나타났다. 본 시험 조건 하에서 이황화메틸의 표적 장기는 관찰되지 않았으며, 무해용량은 암수 모두 25 ppm으로 사료된다. The purpose of this study was to investigate the potential subacute toxicity of dimethyl disulfide by 3 weeks inhalation in F344 rats. The test article, dimethyl disulfide was exposed by inhalation to male and female rats at dose levels of 0, 5, 25, or 125 ppm/6 hrs/ day for 3 weeks. Five rats/ sex/ group were sacrificed on day 4 after the initiation of treatment, while 5 rats/ sex/ group were sacrificed at the end of treatment period. During the test period, clinical signs, mortality, body weights, food consumption, hematology, serum biochemistry, and gross findings were examined. Slight decreases in body weight gain were noted in both sexes of the highest dose group in a dose dependent manner but were only statistically different from the control animals in males of the group. A slight non-significant reduction in food consumption were also noted in the both sexes of the highest dose group. There were no adverse effects on mortality, clinical signs, hematology, serum biochemistry, and necropsy findings at any dose tested. Based on these results, it was concluded that the 3 weeks repeated dose of dimethyl disulfide by inhalation resulted in suppressed body weight gain and decreased food consumption at 125 ppm of both sexes. In the present experimental conditions, the target organ was not determined in rats. The no­observed-adverse-effect level (NOAEL) was considered to be 25 ppm/6 hrs/day for both sexes.

경남지역 전통 된장의 숙성기간에 따른 품질 특성 및 미생물 군집 비교

김현영,김봉신,고희숙,김소영,하기정,Kim, Hyeon-Young,Kim, Bong Sin,Ko, Hee-Suk,Kim, So-young,Ha, Gi-Jeong 한국식품영양학회 2021 韓國食品營養學會誌 Vol.34 No.1

This study investigated the microbial community and quality characteristics of traditional soybean pastes (doenjang) based on the aging period in Gyeongnam province. The samples were collected from seven regions in Gyeongnam Province (Goseong, Hadong, Haman, Hamyang, Sacheon, Sancheong and Uiryeong). Contents of moisture, salinity, pH, and acidity of doenjang after 210 days aging were 56.40~65.21%, 9.05~16.08%, 4.88~6.86 and 0.64~2.14%, respectively. Lightness significantly decreased over the aging period, while the redness tended to increase over the aging period. Yellowness was from 21.39~26.81 to 21.10~28.36. Reducing sugar content was from 0.31~1.45% to 0.11~3.13%. The amino-type content increased from 141.87~495.13 mg/100 g to 328.53~823.67 mg/100 g. In contrast, the ammonia-type content declined from 136.74~364.70 mg/ 100 g to 128.62~331.00 mg/100 g. The overall total microbial count did not significantly differ, but it tended to decrease in sample GD4. Fungus declined overall or was not detected. Coliform groups were not detected in all samples. Lactobacillus and Bacillus cereus tended to decrease over the aging period. The common fungus and bacterium were Aspergillus oryzae and Tetragenococcus halophilus, respectively. After the aging period, the predominant fungi were Candida versatilis, Candida apicola, and Debaryomyces hansenii. The predominant bacteria were Tetragenococcus halophilus and Cronobacter sakazakii.

곡기생(?寄生)의 항염증 효능 및 암세포 이주저해에 미치는 영향

김현영,장수영,정지천,신현철,Kim, Hyun-Young,Jang, Soo-Young,Jeong, Ji-Cheon,Shin, Hyeon-Cheol 대한한방내과학회 2013 大韓韓方內科學會誌 Vol.34 No.1

Objectives : Gokgisaeng (Korean mistletoe) is used for the treatment of inflammatory and cancer diseases in traditional Korean medicine and its major component lectins have been reported to induce nitric oxide (NO) in RAW 264.7 macrophages, and also induce apoptosis of various types of cancer cells, although its modulatory effects on cancer cell migration and macrophage activation is poorly understood. The aim of this study is to clarify molecular mechanisms of action responsible for the anti-inflammatory and antitumor migration potentials of Korean mistletoe extract (KME). Methods : We investigated the anti-inflammatory activity of KME on NO production and inducible nitric oxide synthase (iNOS) expression by lipopolysaccharide (LPS) in both RAW 264.7 macrophages and rat C6 glioma cells, and also evaluated inhibitory efficacy on glioma cell growth and migration. For assessment, XTT assay, nitrite assay, RT-PCR, scratch-wound and Boyden chamber assay, and western blot analysis were performed. Results : Previously reported, unlike the efficacy of Gokgisaeng lectin, KME inhibited NO production and iNOS expression, and suppressed pro-inflammatory mediators including IL-$1{\beta}$, IL-6, COX-2, iNOS in LPS-stimulated RAW 264.7 cells. Furthermore, KME suppressed tumor cell growth and migration, and it also inhibited LPS-induced NO release and iNOS activation by down-regulating expression of protein kinase C (PKC) and phosphorylation of ERK in C6 glioma cells. Conclusions : Our research findings provide evidence that KME can play a significant role in blocking pro-inflammatory reaction and malignant progression of tumors through the suppression of NO/iNOS by down-regulating of inflammatory signaling pathways, PKC/ERK.

Mouse의 신장상피세포에서 패장(敗醬)추출물이 산화 스트레스 및 NF-${\kappa}B$ signaling에 미치는 영향

김현영,장수영,최규호,신현철,Kim, Hyun-Young,Jang, Soo-Young,Choi, Gyu-Ho,Shin, Hyeon-Cheol 대한한방내과학회 2010 大韓韓方內科學會誌 Vol.31 No.1

Objectives : The aims of this study were to investigate the cytoprotective, antioxidative and inflammation genes inhibitory effects of Patriniae Radix on the mouse LLC-$PK_1$ cells (renal epithelial cells). Methods : The cytoprotective effect of Patriniae Radix was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The antioxidative effect was measured in terms of generation amount of superoxide anion radical (${\cdot}{O_2}^-$) by 2',7'-dichlorodihydrofluorescein diacetate (DCFDA), nitric oxide (NO) by 4,5-diaminofluorescein (DAF-2), peroxynitrite ($ONOO^-$) by dihyldrorhodamine 123 (DHR 123) and prostaglandin $E_2$ ($PGE_2$) by $PGE_2$ immunoassay on $H_2O_2$-treated LLC-$PK_1$ cells. For measuring of inflammation genes inhibitory effects, western blot was performed to detect IKK-$\alpha$, phospho-$I{\kappa}B-\alpha$, NF-${\kappa}B$ (p50, p65), COX-2, iNOS, IL-$1{\beta}$ and VCAM-1 protein level in cytosol fractions from LLC-$PK_1$ cells. Results : Patriniae Radix extract reduced the $H_2O_2$-induced cell death and inhibited the amount of $H_2O_2$-induced ${\cdot}{O_2}^-$, NO, $ONOO^-$, $PGE_2$ generation dose-dependently on the mouse LLC-$PK_1$ cells in vitro. Also Patriniae Radix extract inhibited the expression of IKK-$\alpha$, phospho-$I{\kappa}B-\alpha$, COX-2, iNOS, IL-$1\beta$ and VCAM-1 genes dose-dependently by means of decreasing activation of NF-${\kappa}B$. Conclusions : According to above results, it was identified that Patriniae Radix had the cytoprotective, antioxidative and inflammation genes inhibitory effects. So it was suggested that Patriniae Radix would be effective to the treatment for the inflammatory process and inflammation-related diseases.

제주 지역 여성청년의 독립 경험에 관한 공간정치적 접근

김현영(Kim Hyeon-Young),김태연(Kim Tae-Yeon) 한국공간환경학회 2021 공간과 사회 Vol.31 No.4

본 연구는 제주 지역 여성청년의 독립 실천을 다루면서 중심부와 주변부의 공간위계가 상상되고 경합되는 과정의 역동을 살펴본다. 농촌보다는 도시를, 지방보다는 서울을 열망하는 공간위계 속에서 지역 청년들의 변화하는 생애주기에 대한 인식과 삶의 맥락은 어떻게 구성되고 있을까? 중심부와 주변부 공간의 물질적이고 상징적인 격차가 심화되는 가운데 지역, 젠더와 계급이라는 범주는 어떻게 각축하고 있을까? 이 질문에 답하기 위해, 본 연구는 독립과 의존을 대립적인 것으로 상정하는 대신 여성청년들의 독립 실천을 ‘과정적인 것’으로 규정하고, “잔류”로 표상되는 삶의 맥락 속에서 자신만의 공간을 확보하려는 다양한 실천을 살펴본다. 이들의 독립 실천은 중앙과 지방, 이주와 정주라는 이중의 위계 속에서 현재적 사건으로 긍정되지 못하며 다양한 구조적 곤란에 맞닥뜨리게 된다. 그럼에도 이들은 다양한 공간전략을 구사하며 새로운 장소를 만들어낸다. 관계적 장소로서 새롭게 집을 장소화하는 이들의 실천에는 중앙-지방의 공간위계를 새롭게 사유할 가능성이 잠재한다. This paper, considering the young unmarried women’s experiences of making their own space in Jeju Island, explores the ways of interpreting the hierarchical dualism of geographical center and periphery in South Korea. Our research question is how the young women who live in Jeju perceive and interpret their own life context, and then how they cope with spatial hierarchy between the urban and the rural, and between Seoul and the local. In some sense, their encounter with the spatial hierarchy cannot but help break through the effects of intermingling social forces such as locality, gender and class. The deepening gap of material (economic) opportunities and of symbolic power between the central and peripheral region is a big challenge to the young generation in Jeju. We define their practices of making their own living spaces as ‘processive’ and regard these as ‘remaining’ in their living context. Their practices are neglected in the cultural circumstance that forces the young generation to sacrifice the present for the future. That sacrifice is justified as an investment. It looks like an structurally imposed obstacle. Nevertheless, the young women, particularly unmarried women try to reclaim new places through negotiating between their position within the structure and their own strategic choice. It can be their detour through which they rethink the spatial hierarchy.

수컷 랫드(Sprague-Dawley)에서 2-부톡시에탄올(2-butoxyethanol)의 단회 기도내 투여에 따른 급성 독성시험

김현영 ( Hyeon-young Kim ),김인현 ( In-hyeon Kim ),김민석 ( Min-seok Kim ),김성환 ( Sung-hwan Kim ),이규홍 ( Kyuhong Lee ) 한국산업보건학회 2021 한국산업보건학회지 Vol.31 No.4

Objectives: The present study aimed to evaluate the potential toxicity of 2-butoxyethanol after intratracheal instillation in male rats. Methods: In order to calculate median lethal dose (LD₅₀) of 2-butoxyethanol using Probit analysis with SAS program, the 2-butoxyethanol was administered with dose levels of 0, 101.64, 203.28 and 406.56 mg/kg by once intratracheal instillation to male rats. During the test period, clinical signs, mortality, body weights, organ weights, hematology, and serum biochemistry were examined. At the end of 14 days observation period, all animals were sacrificed and gross finding and histopathological examination were performed. Results: All animals of 406.56 mg/kg group died within 2 weeks after the administration of 2-butoxyethanol. Treatment-related clinical signs, gross observation and histopathological changes (mucous cell hyperplasia, alveolar macrophage aggregation, and hemorrhage) of lung exhibited an increased in 2-butoxyethanol treated groups in a dose dependent manner. However, there were no changes in the organ weights, hematology and serum biochemistry, and histopathology of any other organ except lung. Conclusions: On the basis of the results, it was concluded that a single intratracheal instillation of 2-butoxyethanol in male Sprague-Dawley rats resulted in some adverse effects on mortality, clinical sign, and histopathology in the lung. In the experimental conditions, the LD₅₀ of 2-butoxyethanol was considered to be 287.2 mg/kg and lung was founded to be the target organ of 2-butoxyethanol.

‘사회재생산의 위기’를 둘러싼 페미니즘 논의의 검토

김현영(Kim, Hyeon-Young) 한국여성연구소 2022 페미니즘 연구 Vol.22 No.2

이 글은 오늘날 사회재생산의 위기를 이해하기 위해 페미니즘 문헌에서 사회재생산에 관한 이론적 논의를 검토한다. 우선, 가사노동 논쟁을 거쳐 분석적 프레임워크로서 사회재생산 개념의 발전과정을 고찰한다. 이렇게 도출된 사회재생산 관점을 통해 신자유주의적 지구화와 변화하는 젠더질서 속에서 이중화되는 사회재생산의 양상을 검토한다. 이는 탈젠더화되고 탈인종화된 특성을 표방하는 신자유주의적 경제가 사실상 젠더, 인종, 계층과 시민권의 교차 속에서 위계적으로 구성되는 사회재생산 노동에 깊숙이 의존하고 있음을 드러낸다. 오늘날 재생산노동의 위계적 조직화가 여성내부의 격차를 체계적인 방식으로 결합하며 이루어진다는 사실은 방법론적 일국주의를 넘어선 교차적인 사회재생산 분석의 발전을 요구한다. 마지막으로 이 같은 이론적 통찰이 생산과 재생산의 경계를 문제삼는 페미니즘 정치에 제기하는 시사점을 간략히 살펴본다. This study examines the trajectory of ‘social reproduction’ in the context of feminist literature since the domestic labour debate. It analyzes the privatization of social reproduction and shifting gender order in the neoliberal era and emphasizes that the neoliberal economy which claims to be degendered and deracailized, is highly dependent on the hierarchical construction of social reproductive labour with the intersection of gender, race class, and citizenships. It demands elaborations of intersectional social reproduction analysis beyond methodological nationalism. Finally, it suggests these theoretical insights could be useful resources for feminist politics.

〈박씨전〉을 활용한 문학치료 사례연구

김현영(Kim Hyeon-Young) 강원대학교 인문과학연구소 2010 인문과학연구 Vol.0 No.25

본고는 고소설 〈박씨전〉을 활용하여 문학치료 상담을 한 사례에 대한 보고이다. 2장에서는 〈박씨전〉에 나타난 서사의 양상을 살펴보았다. 배우자 감싸기 유형의 부부서사가 나타나며, 융의 분석심리학을 적용하여 분석한 결과 아니마의 회복이라는 점에서 자기실현에 이르기 위한 과정을 찾아볼 수 있었다. 따라서 〈박씨전〉에는 자기성숙의 지표가 나타나며 인간관계에서의 성공을 그려내는 서사가 나타난다고 볼 수 있다. 3장에서는 〈박씨전〉을 활용한 문학치료 상담 과정을 제시하였다. 부부서사영역에서의 자기서사를 진단하고 문제적 서사를 작품서사를 통하여 보완하여 치료하는 것을 목표로 하였다. 고등학교 재학생 2명을 대상으로 '예비→진단→치료→마무리'의 과정으로 진행하였다. 두 학생 모두 〈박씨전〉에 나타난 배우자 감싸기 서사를 거부하고 배우자가르기 유형의 자기서사를 가지고 있었는데, 치료 단계를 거쳐 자기서사의 변화를 보였다. 치료 단계에서는 징검다리 서사로서 영화 〈페인티드베일〉을 활용하였다. 영화는 부부 관계에서의 위기와 해결 방안이 여러 측면에서 제시되고 있는데, 이러한 작품 서사를 접하면서, 〈박씨전〉에 나타난 배우자 감싸기 서사를 받아들이는 쪽으로의 변화를 보였다. 또한 생애담 다시쓰기 활동을 통해서도 부부서사의 부분에서의 변화를 확인할 수 있었다. 이번 사례를 통해 〈박씨전〉을 활용한 문학치료 활동이 어느 정도 타당성과 유용성을 갖는다고 볼 수 있겠다. This study is a case report about Literary therapy counseling of Bakssijeon. In this study, we discussed the aspects of epic found in Bakssijeon. First of all, in this novel, we could see husband and wife epic; the epic of protecting mate, and second, self-actualization epic, which resulted from the analysis based on Jung's analytical psychology. Consequently, this shows that there is the epic of a success on human relationships in Bakssijeon. This study also examined the process and result of the literary therapy counsel using Bakssijeon. In this case, the subjects were three high school students, and the process was four steps of 'preparation → diagnosis → therapy → finish'. The students, at first, had self-epic of the dividing mate, rejecting the epic of protecting mate on Bakssijeon. However, their self-epic has changed step by step passing through the therapy stage. In this stage, we made use of a stepping stone epic, a movie The Painted Veil. Experiencing the movie, the students changed their attitude, eventually accepted the protecting mate epic of Bakssijeon. Finally, with the activity of writing their own lifetime, students showed some changes in their self-epic. With this case, we found that the literary therapy using Bakssijeon is valid and useful to some extent.

간호대학생들의 시스템 사고 기반 천식환자 간호과정 분석

김현영 ( Hyeon Young Kim ),오현수 ( Hyun Su Oh ),윤은경 ( Eun Kyoung Yun ) 한국시스템다이내믹스학회 2013 한국시스템다이내믹스 연구 Vol.14 No.2

The purpose of this study is to analyze nursing students` proficiency with ST(Systems Thinking) approach concerning asthma patient nursing process. For this study, 20 nursing students created causality cognitive maps which were consisted of the contents of ‘asthma nursing care``. The word association and causality cognitive map were used to estimate ability of the ST. As a result, the students failed to understand the nursing process with the ST approach, or alternatively they applied low level ST approach and had a lower understanding of it. The findings of this study suggest that the development of a ST-based nursing educational program in order for nursing students to improve their ST ability as well as knowledge integration for multidisciplinary practice.

수술 환아의 불안에 적용한 비약물적 중재의 효과: 체계적 문헌고찰 및 메타분석

김현영 ( Kim Hyeon-young ),신선화 ( Shin Sun Hwa ) 경희대학교 동서간호학연구소 2021 동서간호학연구지 Vol.27 No.1

Purpose: The purpose of this study was to examine the effectiveness of non-pharmacological interventions for reducing perioperative anxiety in children undergoing surgery. Methods: A systematic review of randomized controlled trials (RCTs) with the primary outcome of children’s perioperative anxiety was conducted. The literature search was performed using various databases, including Cochrane Library, CINAHL, EMBASE, PubMed, and Korean electronic databases with confined to RCTs between 2000 and 2020. A total of sixteen studies were suitable the inclusion criteria and were systematically reviewed. The bias risk of randomized studies was evaluated using Cochrane’s risk of bias tool. For the meta-analysis, RevMan 5.4 was used to analyze effect sizes of interventional factors. Results: Finally, twelve RCTs studies were used for meta-analysis. The non-pharmacological interventions implemented to reduce perioperative anxiety in children were therapeutic play, clown therapy and information provision. First, therapeutic play had a significant effect on reducing preoperative anxiety, with an effect size of -1.46 (95% CI=-1.78~-1.14). Second, clown therapy had a significant effect on reducing preoperative anxiety, with an effect size of -0.97 (95% CI=-1.45~-0.49). Finally, the provision of information had a significant effect on reducing preoperative anxiety, with an effect size of -0.75 (95% CI=-0.99~-0.51). Conclusion: This meta-analysis suggests that non-pharmaceutical interventions provide effective methods of reducing perioperative anxiety in children. Therefore, the findings verify evidence that various non-pharmacological interventions are effective means for reducing children’s preoperative anxiety.