말단비대증 환자의 뇌하수체 종양조직에서 H - ras 유전자 변이의 가능성

임승길(S . K . Lim),권이현(Y . H . Kwon),정윤석(Y . S . Chung),안광진(K . J . Ahn),이은직(E . J . Lee),김경래(K . R . Kim),이현철(H . C . Lee),허갑범(K . B . Huh),김태승(T . S . Kim) 대한내과학회 1993 대한내과학회지 Vol.45 No.3

N/A Backround: Little is known about the mechanism of tumorigenesis in pituitary adenomas. An important finding in somatotroph adenomas is that a somatic mutation may convert a G protein, Gs(α) into a putative oncogene termed gsp via point mutations at two critcal sites. The ras protooncogenes are structurally related to the G-protein family and are involved in cell proliferation and differentiation. Although ras oncogene mutations have been indentified in a wide variety of human neoplasm, only one case was reported as containting single point mutation in a patient with invasive prolactinoma, In this report we used oligonucleotide-specific hybridization to screen ras mutations in 13 acromegalic tumors. Methods: Pituitary tissue samples were derived from a central portion of the paraffin embedded pituitary tumor to minimize the possibility of contamination with normal tissue. Genomic DNA was isolated and purified from tumor tissue and amplified by the standard PCR method. Amplified DNAs from each of the region of H-ras genes (12/13 and 61) were analyzed for potential ras mutations using oligonucleotide-specific hybridization as described previously. Results: Wild type radiolabelled oligoncleotides were hybridized to the amplified DNAs from the patients' tumor and to the positive specimens. They were, however, easily striped out at 68℃ by nonstringent washing procedures except control (wild type) specimens. All radiolabelled mutant oligonucleotides could be easily striped out of 13 specimens except a control mutant specimen by the same procedure. Conclusion: We could not find any H-ras mutation that might not be frequently found in acromegalic patients, and that gsp (Gsa mutation) or mutations in the PKA system-related proteins might be the main oncogene in acromegalic patients. However further efforts to find the other somatic mutations including K-ras and N-ras should be given to these patients for more precise understanding of pathogenesis and for planning of the better treatment.

섹터 캐쉬 메모리의 효율적인 Trace-Driven 시뮬레이션 알고리듬

김경래(K. R. Kim),유석근(S. K. Yoo),허용민(Y. M. Hur),박동규(D. G. Park),임인칠(I. C. Lim) 대한전자공학회 1991 대한전자공학회 학술대회 Vol.1991 No.6

디렉토리 베이스 캐쉬 코히어런스 기법에 대한 Trace-driven Simulation

유석근(S. K. Yoo),김경래(K. R. Kim),허용민(Y. M. Hur),윤용호(Y. H. Yun),박동규(D. G. Park),임인칠(I. C. Lim) 대한전자공학회 1991 대한전자공학회 학술대회 Vol.1991 No.6

스마트 팩토리 구현을 위한 Windows 10 IoT 기반의 고정밀 로봇 컨트롤러 개발

유기현(G. H. You),김경래(K. R. Kim),이정완(J. W. Lee) 한국정밀공학회 2017 한국정밀공학회 학술발표대회 논문집 Vol.2017 No.12월

한국인에서의 비전형적 당뇨병

이은직(Eun J . Lee),김도영(Doe Y . Kim),허갑범(Kap B . Huh),김현만(Hyeon M . Kim),임승길(Sung K . Lim),김경래(Kyung R . Kim),이현철(Hyun C . Lee),김덕회(Duk H . Kim) 대한내과학회 1987 대한내과학회지 Vol.33 No.6

N/A Of 501 Korean diabetics admitted to Severance Hospital, Yonsei University College of Medicine, non-obese non-insulin-dependent diabetes mellitus (NIDDM) comprised 319 cases (63.7%), obese NIDDM 100 cases (19.9%) and insulin-dependent diabetes mellitus (IDDM) 82 cases (16.4%). And in the IDDM group thus collected, juvenile-onset diabetes (JOD) comprised 17 cases (3.4%), youth-onset underweight diabetes (YOUD) 25 cases (5.0%) and maturity-onset underweight diabetes (MOUD) 40 eases (8.0%). The caloire intakes just before the onset of diabetes in YOUD and MOUD patients were found to be 63.1% and 60.9% and the intakes of protein 55.9% and 79.8% of the recommended dietary allowance, respectively. Among JOD, YOUD and MOUD, there were no significant differences in body weight, and fasting blood glucose and HbA1c levels at the time of admission. The basal and peak serum C-peptide levels in YOUD (1.03±0.61ng/ml, 1.59±1.23ng/ml) were slightly higher than those in JOD (0.64±0.30ng/ml, 1.05±0.54ng/ml), but significantly lower than those in MOUD (1.61±0.73ng/ml, 2.65±1.30ng/ml). Between JOD and YOUD, there was no significant difference in family histories (23.5%, 24.0%) or clinical features. However, MOUD turned out to have significantly lower family history (2.5%), no history of diabetic ketoacidosis and more pulmonary tuberculosis than the former 2 groups. Of 29 young diabetics (JOD and YOUD) who were followed-up at OPD, 14 cases (5 JOD and 9 YOUD) were ketosis-resistant. These data suggest that, in Korea which is located in temperate zone, malnutrition-related diabetes mellitus (MRDM) can be mainly classified into 2 groups: The youth-onset one had the characteristics of IDDM with the history of undernutrition and ketosis-resistance, and the maturity-onset one may belong to NIDDM with the higher susceptibility to pulmonary tuberculosis.

갑상선 질환에 있어서의 골밀도의 변화

전근재(G . J . Jeon),임승길(S . K . Lim),이현철(H . C . Lee),김경래(K . R . Kim),허갑범(K . B . Huh) 대한내과학회 1988 대한내과학회지 Vol.35 No.6

N/A We studied 59 patients who had received a diagnosis of graves' disease or myxedema by dual photon absorptiometry from August 1986 to July 1987 at the Department of Internal Medicine, Yonsei University Severance Hospital. The results were summarized as follows: 1) Patients with Graves' disease of a mean duration of 19.2 weeks demonstrated an average of 9.37 mg/dl Ca and 4.29 mg/dl P in their blood samples while the 24 hr urine specimen showed 194.26 mg/dl 24 hr of Ca and the P revealed 520.36 mg/24 hr, which is higher than those of myxedema. 2) The patients with Graves' disease revealed a prevalance of osteoporosis in the following, Ward's triangle 18,8%, femora) neck 20.8%, trochanter 25%, but the spine, while observed at 22.9% in hyperthyroidism, also was detected at 18.2% in myxedema. 3) In females with Graves' disease, the values of BMD were relatively lower than those of the control group but showed a greater at traction to osteoporosis than the males. 4) Most womon in their postmonopaunal poriod showed a strong tondency to develop osteoporosis in the femoral neek but they demonstrated a greater relationship to the spine. 5) Even after treatment, the BMD revealed a tendency to continue increasing, however, there was still a possibility for incomplete recovery. Excess thyroid hormones lead to negative bone mineral balance. The revealed bone loss was clinically significant in postmenopausal women despite its short dyratuib abd ut aooeared ti be at keast oartuakkt reversubke after treatment.

고종횡비 패턴을 가지는 수직형 프로브카드용 가이드플레이트 제조방법

박태훈(T. H. Park),송인협(I. H. Song),신소원(S. W. Shin),김경래(K. R. Kim),박만석(M. S. Park) 한국생산제조학회 2022 한국생산제조시스템학회 학술발표대회 논문집 Vol.2022 No.12

한국 성인에서 비알콜성 지방간의 중증도에 따른 대사이상의 특징

김형진,김대중,김수경,김세화,이유미,안철우,차봉수,송영득,임승길,김경래,이현철,허갑범 대한내분비학회 2002 Endocrinology and metabolism Vol.17 No.4

연구배경: 30세 이상의 성인 한국인에서 지방간의 중증도와 대사증후군의 여러 요소들 (특히 인슐린저항성 및 중심성비만)과의 관련성을 분석하고자 하였다. 방법: 대상자는 2001년 4월부터 6월까지 건강검진센터를 방문한 사람들 중 만성 바이러스성 간염이나 일주일에 2회 이상 음주력이 있는 사람들을 제외한 1074명 (남자 502, 여자 572)에서 문진, 신체검사, 채혈 그리고 복부초음파를 시행하였다. 결과: 1074명중 522명에서 지방간이 양성이어서 양성율은 49%였으며 남성은 여성에 비해 높은 유병률을 보였다. (57 vs 42%, p<0.05). 당뇨병, 비만 그리고 이상지질증을 가지지 않은 사람들에서 비알콜성 지방간의 유병률은 20%였다. 지방간이 양성인 522명중 218명은 경도, 273명은 중등도, 31명은 중증의 지방간 소견을 보였다. 체질량지수, 허리둘레, 체지방률, 수축기혈압, aspartate aminotransferase, alanine aminotransferase, 총콜레스테롤, 중성지방, 그리고 총콜레스테롤/고밀도지단백 비는 지방간의 중증도에 따라 의미있게 증가하는 양상을 보였다(p<0.05). 다중회귀분석을 시행하였을 때 지방간의 중증도를 잘 반영하는 인자로는 허리둘레, alanine aminotransferase, HONA_IR, 총콜레스테롤/고밀도지단백비, aspartate aminotransferase, 그리고 수축기혈압의 순서로 나타났다. 공복혈당장애, 고혈압 및 인슐린저항성은 지방간을 가진 군에서 대조군보다 의미있게 높은 빈도를 보였을 뿐만 아니라 지방간의 중증도에 따라 차이를 보였다(p<0.05). 인슐린저항성은 심한 지방간에 비교위험도가 14.7(CI; 6.8∼32.0), 중등도의 지방간에서 6.9(CI; 4.6∼10.3), 경도의 지방간에서 5.7(CI; 3.6∼8.8)이었으며 HOMA_IR의 상위 1/4을 인슐린저항성이 있는 군으로 정의하였을 때 각군에 인슐리저항성을 가진 비율은 7.6, 32.0, 36.0, 그리고 55%였다(p<0.05). 지방간의 중증도에 따른 비알콜성 지방간염의 고위험군 비율은 각각 5.0, 21.6, 27.8, 그리고 58.1%였다(p<0.05). 결론: 우리나라의 비알콜성 지방간의 빈도는 서구인에 비해 높은 것으로 나타났다. 또한 비알콜성 지방간이 있을 경우 대사증후군과 관련된 여러인자들(특히 인슐린저항성과 중심성비만)과 비알콜성 지방간염의 위험이 현저히 증가하며 초음파의 중증도 분류에 따라 비례하였다. Background: We evaluated the frequency of non-alcoholic fatty liver disease, and the associations between the metabolic abnormalities and severity of non-alcoholic fatty liver disease in Korean adults using ultrasonography. Methods: We examined 1074 Korean adults above the age of 30 years, comprising of 502 men and 572 women, participating in medical check-ups at the Health Promotion Center. Hepatitis B and C serologies were negative, and the average weekly alcohol intake was ≤ 2 standard drinks. A standard interview, physical exam and biochemical study, were conducted, and an experienced operator carried out ultrasound liver studies. Results: 522 of the subjects had non-alcoholic fatty liver disease, and the remaining 552 did not. The frequency in the men was higher than that in the women (57 vs 42%, p<0.05). The frequency of non-alcoholic fatty liver disease in the subjects without diabetes, obesity or dyslipidemia was 20%. We classified subjects into 4 groups: the controls (n=552) and those with mild (n=218), moderate (n=273) or severe fatty liver disease (n=31), according to their ultrasonographic findings. BMI, waist circumference, body fat, systolic blood pressure, aspartate aminotransferase, alanine aminotransferase, total cholesterol, triglyceride, the total-to HDL-cholesterol ratio, impaired fasting glucose, hypertension and insulin resistance, were all significantly increase with the increased severity of non-alcoholic fatty liver disease (p<0.05). Following the multiple regression analyses, waist, alanine, aminotransferase, HOMAIR, the total- to HDL-cholesterol ratio, aspartate aminotransferase and systolic blood pressure, were all associated with the severity of non-alcoholic fatty liver disease. Odd ratios of insulin resistance in the mild, moderate and severe non-alcoholic fatty liver disease were 14.7 (CI: 6.8∼32.0), 6.9 (CI: 4.6∼10.3) and 5.7 (CI 3.6∼8.8), respectively. The percentages of subjects with insulin resistance in each group were 7.6, 32.0, 36.0 and 55.0% (p<0.05), respectively. The percentages of subjects with risk of non-alcoholic steatohepatits in each groups were 5.0, 21.6, 27.8 and 581% (p<0.05) respectively. Conclusion: The frequency of non-alcoholic fatty liver disease was relatively high in Korean adults. Proportional differences in metabolic abnormalities, relation to the severity of non-alcoholic fatty liver disease, were found by ultrasonography (J Kor Soc Endocrinol 17:514∼525, 2002).

인슐린 자가 항체에 의해 유발된 저혈당증 1예

임승길,김경래,이수곤,이관우,황성오,허갑범,이현철,이혁우 대한내분비학회 1989 Endocrinology and metabolism Vol.4 No.1

Autoimmune hypoglycemia is characterized by hyperinsulinemia, fasting hypoglycemia, and presence of insulin autoantibody without previous exposure to exogenous insulin. Though this conditions were reported exclusively in Japan, we recently experienced the first case of autoimmune hypoglycemia in Korea and present it with a literature review. (J Kor Soc Endocriuol, 4: 72~76, 1989)

당뇨병의 병형에 따른 감염증의 양상

이현철,허갑범,이이형,임승길,김현만,김경래 대한당뇨병학회 1988 Diabetes and Metabolism Journal Vol.12 No.1