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The Effect of Oryzalin on Growth and Gravitropism in Arabidopsis Roots
Jin Gyu Go(고진규),Sun Ill Park(박선일),Soon Young Kim(김순영) 한국생명과학회 2021 생명과학회지 Vol.31 No.2
Oryzalin은 미세소관의 배열을 방해하는 dinitroaniline계 제초제이다. 미세소관과 미세섬유는 식물 세포 생장에 관여하는 세포벽을 구성하는 골격 성분이다. 또한 미세소관은 평형석의 침전에도 관여하는데, 이는 뿌리 끝에 있는 columella 세포에서 중력 인지를 조절한다. 본 연구는 애기장대 뿌리에서 에틸렌 생성을 통하여 oryzalin이 뿌리 생장과 굴중성 반응에 미치는 영향을 조사하였다. 10<SUP>-4</SUP> M oryzalin을 뿌리에 처리하면 뿌리 생장과 굴중성 반응이 완전히 억제되었다. 뿌리 생장과 굴중성 반응을 조절하는 oryzalin의 작용을 알아보기 위해 애기장대 뿌리절편에서 ethylene 생합성을 측정하였다. Oryzalin을 처리하면 ACC oxidase와 ACC synthase을 활성을 촉진하여 에틸렌 생성이 촉진되고, 증가된 ethylene은 미세소관의 배열을 변화시켜 뿌리 생장과 굴중성 반응을 억제한다. Oryzalin is a dinitroaniline herbicide that has been known to disrupt microtubules. Microtubules and microfilaments are components of cytoskeletons that are implicated in plant cell growth, which requires the synthesis of cellulose when cell walls elongate. In addition, microtubules are also involved in the sedimentation of statoliths, which regulate the perception of gravity in the columella cells of root tips. In this study, we investigated the effect of oryzalin on the growth and gravitropic response of Arabidopsis roots. The role of ethylene in oryzalin’s effect was also examined using these roots. Treatment of oryzalin at a concentration of 10<SUP>-4</SUP> M completely inhibited the roots’ growth and gravitropic response. At a concentration of 10<SUP>-6</SUP> M oryzalin, root growth was inhibited by 47% at 8 hr when compared to control. Gravitropic response was inhibited by about 38% compared to control in roots treated with 10<SUP>-6</SUP> M oryzalin for 4 hr. To understand the role of oryzalin in the regulation of root growth and gravitropic response, we measured ethylene production in root segments treated with oryzalin. It was found that the addition of oryzalin stimulated ethylene production through the activation of ACC oxidase and ACC synthase genes, which are key components in the synthesis of ethylene. From these findings, it can be inferred that oryzalin inhibits the growth and gravitropic response of Arabidopsis roots by stimulating ethylene production. The increased ethylene alters the arrangement of the microtubules, which eventually interferes with the growth of the cell wall.
Deep Learning in MR Image Processing
이두희,이진구,고진규,윤재연,류강현,남윤호 대한자기공명의과학회 2019 Investigative Magnetic Resonance Imaging Vol.23 No.2
Recently, deep learning methods have shown great potential in various tasks that involve handling large amounts of digital data. In the field of MR imaging research, deep learning methods are also rapidly being applied in a wide range of areas to complement or replace traditional model-based methods. Deep learning methods have shown remarkable improvements in several MR image processing areas such as image reconstruction, image quality improvement, parameter mapping, image contrast conversion, and image segmentation. With the current rapid development of deep learning technologies, the importance of the role of deep learning in MR imaging research appears to be growing. In this article, we introduce the basic concepts of deep learning and review recent studies on various MR image processing applications.
원발 및 전이 갑상선 유두암에서의 NA(+) /I(-) Symporter mRNA 표현양상의 임상적 의의
이은주,이성진,박현주,김상희,고진규,김성배,안일민,홍석준,박기영,김하영,공경업 대한내분비학회 1999 Endocrinology and metabolism Vol.14 No.3
Background: The iodide transport into thyroid cells is an essential step in the biosynthesis of thyroid hormones. The sodium iodide symporter (NIS) which is responsible for iodide transport was cloned recently and identified as a plasma membrane glycoprotein. Recent report suggested the absence of human NIS (hNIS) mRNA expression of papillary carcinoma in thyroid indicates absence of response on radioiodine therapy for distant metastasis. To understand the change of hNIS expression at the stage of metastasis in papillary thyroid carcinomas, we evaluated the expression levels of hNIS mRNA in primary and lymph node metastatic papillary carcinoma tissues. Methods: Seven pairs of primary and lymph node metastatic tissues were included in this study. The level of hNIS mRNA in lymph node metastatic tissues and primary tissues were evaluated by reverse transcriptase-polymerase chain reaction (RT-PCR). The level of GAPDH mRNA was used as internal control. Results: Two among 6 lymph node metastatic tissues did not show hNIS mRNA even with significant hNIS expressions in papillary carcinoma tissues in thyroid. The levels of hNIS expression of remaining 4 lymph node metastatic tissues were lower than those of corresponding primary tissues. Interestingly, one case showed no hNIS expression in primary tissue, but significant hNIS expression in lymph node metastatic tissue. There was no correlation in hNIS mRNA expression between primary and lymph node metastatic tissues. Conclusion: No correlation was found in hNIS mRNA expression between primary and lymph node metastatic tissues, suggesting the measurements of hNIS mRNA level in primary tissues may not predict therapeutic response to radioactive iodine (J Kor Soc Endocrinol 14:514-519, 1999).
정상적인 위내시경 소견을 보이는 사람의 위점막 조직에서 cagA 유전자 존재의 의의
박주상,윤광희,홍원선,남승우,민영일,정훈용,고진규,김해련,양석균,이미헌,강경훈 대한소화기학회 1998 대한소화기학회지 Vol.31 No.6
Background/Aims: It becomes clear that clinical manifestation of H. pylori infection has marked diversity mainly due to the strain diversity of H. pylori and host susceptibility. Many attempts have been made to identify the pathogenic strains of H. pylori, and have shown that the strain with the gene coding for cagA may be a pathogenic strain. To determine the role of cagA gene in the developrnent of gastroduodenal diseases, it is important to test cagA gene in gastric tissues without gross abwrmality. Methods: In a total of forty-seven persons without abnormal gastroscopic findings, the prevalence of H. pylori was determined by polymerase chain reaction (PCR), CLO test, culture, and histological examination. Genomic DNA was amplified by PCR using the primer specific for the 109-bp product of 16S rRNA gene of H. pylori. The prevalence of cagA gene was examined in 37 persons who were positive in PCR for 16S rRNA gene of H. pylori. The PCR product using primer set specific for cagA gene was a 350-bp sized and represented mid-region of cagA gene. Resnlts: Thirty-two (68.1%) out of 47 persons were infected by H. pylori. Thirty-seven (78.7%) persons showed positive PCR result for H. pylori. The cagA was identified in 28 (75.7%) among 37 H. pylori positive persons. Conclusions: The high prevalence of cagA gene in H, pylori-infeced gastric mucosa was observed in the persons with no specific gross abnormality in gastroscapic examination. These results indicate that the expression of cagA gene is common in H. pylori infected gastric mucosa in Korea. To canfirm the cytotoxie activity, the further studies using other primers are needed.
김진호,박소연,김명환,박원철,이호정,이성구,주영민,서동완,송병철,민영일,유교상,고진규,한상택,정세라,주연호 대한소화기내시경학회 2000 Clinical Endoscopy Vol.20 No.2
An anomalous pancreaticobiliary ductal union is a rare anomaly occurring in the biliary tract. This anomaly has been implicated as a cause of choledochal cysts, bile duct and gallbladder carcinoma, and recurrent pancreatitis. A case is herein reported of a 63 year-old woman who suffered from gallbladder cancer associated with a choledochal cyst and an anomalous pancreaticobiliary union.
김해경,홍성수,강경훈,홍원선,민영일,정훈용,박의련,김해련,양석균,심기남,고진규 대한소화기내시경학회 1999 Clinical Endoscopy Vol.19 No.5
Granular cell tumors of the gastrointestinal tract are uncommon, and the esophagus is the gastrointestinal site most frequently affected. Such tumors are rarely seen in the stomach, colon, or rectum. Azzopardi first described a granular cell tumor of the stomach in 1956. Since then a few gastric granular cell tumors have been reported in corresponding literature. It is believed that there have been no reported case of a granular cell tumor of the stomach in Korea. Subsequently one case of a granular cell tumor of the stomach in 38 year-old female who complained epigastric soreness is herein reported, and was successfully managed by endoscopic resection.