고등학교 생물실험 개선방안에 관한 연구

고준상,홍성식 全南大學校 師範大學 科學敎育硏究所 2005 科學敎育硏究誌 Vol.29 No.1

The detection of fats is carried out easily by the method which Benzene in the solution including fat reacts on SudanⅢ. I put 0.01% Nile blue liquid instead of SudanⅢ and find it's the best way to check the result of the experiment, because it changes the color of the solution from blue to red. When plasmolysis and osmosis are studied, if cells are dyed by Methylene blue, and the plasmolysis is appeared more clearer than before. when using Hydrilla verticillata, protoplasm is green, it is easier to check the plasmolysis. In case of osmosis, if semi -permeable membrane is used, it is the most efficient condition for this experiment that cellophane papers and onion peelings are used as semi-permeable membrane, and sucrose solution is used as osmosis solution. When photosynthetic pigment isolation is studied, pigment extractions are centrifuged and put it on chromatography paper. This makes the line of pigment clear and saves the time for experiment. To get the Retention Factor(Rf), pasting the pigment on RP-TLC by the blade of a coin is more efficient than on chromatography paper. When mitosis is studied, it is hard to get a root from a new onion growing up in water, and it takes at least more than 10 days. However, when I use a root tip germinated from onion seed, it is possible to complete the experiment only in three days. In addition to this, it is good for observing chromosome to use Aceto-Orcein, Toluidine blue and PAS as dying solution.

鐵筋콘크리트 板요소의 非線型解析

高準祥,崔正鎬,申鉉穆 대한토목학회 1995 대한토목학회 학술발표회 논문집 Vol.1995 No.1

PS콘크리트 판요소의 비선형해석에 관한 연구

高準祥(고준상),崔正鎬(최정호),申鉉穆(신현목) 대한토목학회 1996 대한토목학회 학술발표회 논문집 Vol.1996 No.1

LCSeq를 이용한 변형 웜 시그니쳐 생성 엔진 구현

고준상(Joon-Sang Ko),김봉한(Bong-Han Kim),이재광(Jae-Kwang Lee) 한국콘텐츠학회 2007 한국콘텐츠학회논문지 Vol.7 No.11

본 논문에서는 알려지지 않은 변형 웜을 탐지하기 위한 방법을 제안한다. 그 방법으로, 페이로드 영역에서 시그니쳐 생성 방안들을 패턴인식 알고리즘으로 연구되었던 Suffix Tree중에서 Longest Common Subsequence(LCSeq) 기법을 이용하여 새로운 시그니쳐를 자동적으로 생성할 수 있는 프로그램을 설계하여 구현하였다. 테스트를 통해 코드레드 웜과 님다 웜의 변종을 검출하는 과정을 보여주고 기존 snort의 시그니쳐와 LCSeq를 이용해 생성된 시그니쳐를 비교ㆍ평가하였다. We introduce the way to detect the mutation worm. We implemented the program that can generate signature using LCSeq(Longest Common Subsequence) technique in Suffix Tree studied as pattern recognition algorithm. We also showed the process to detect the mutation of CodeRed worm and Nimda worm and evaluated signatures generated by snort and LCSeq.

청풍대교(복합사장교)의 설계와 시공

유동호,고준상,박태균,김도균,Yoo, D.H.,Ko, J.S.,Park, T.K.,Kim, D.K. 한국전산구조공학회 2010 전산구조공학 Vol.23 No.1

Bile Acid Analysis in Biliary Tract Canacer

박정엽,박병규,고준상,방승민,송시영,정재복 연세대학교의과대학 2006 Yonsei medical journal Vol.47 No.6

The etiology of biliary tract cancer is obscure, but there are evidences that bile acid plays a role in carcinogenesis. To find the association between biliary tract cancer and bile acid, this study compared the bile acid concentration and composition among patients with biliary cancer, biliary tract stones, and no biliary disease. Bile was compared among patients with biliary tract cancer (n = 26), biliary tract stones (n = 29), and disease free controls (n = 9). Samples were obtained by percutaneous transhepatic biliary drainage, endoscopic nasobiliary drainage, or gallbladder puncture, and analyzed for cholic, deoxycholic, chenodeoxycholic, lithocholic, and ursodeoxycholic acid composition. Total bile acid concentration was lower in the cancer group than the biliary stone and control groups; the proportions of deoxycholic (2.2% vs. 10.2% and 23.6%, p 0.001 and p 0.001, respectively) and lithocholic acid (0.3% vs. 0.6% and 1.0%, p = 0.065 and p 0.001, respectively) were also lower. This result was similar when disease site was limited to bile duct or gallbladder. Analysis of cases with bilirubin 2.0 mg/dL also showed lower total bile acid concentration and deoxycholic acid composition in the cancer group compared to controls (5.7% vs. 23.6%, p = 0.003). Although the presence of bile duct obstruction explains some of the difference in total concentration and composition of bile acid, there are other contributing mechanisms. We suspect the alteration of bile acid transport might decrease bile acid excretion and cause the accumulation of carcinogenic bile acid in bile duct epithelium.

비전형적 천식을 동반한 알레르기성 기관지폐 아스페르길르루스증 1례

홍천수,박용범,신동환,박중원,고준상,홍용국,황영웅 대한알레르기학회 1997 천식 및 알레르기 Vol.17 No.3

Recently we experienced an allergic bronchopulmonary aspergillosis with atypical respiratory symptoms. Forty seven years old female was admitted to our hospital because of cough, sputum, right pleuritic pain for 2 weeks. She hsd experienced pneumonia several times, but had been not confirmed any causative organism. On physical examination, breathing sound was decreased over right upper lung, but wheezing and crackle were not heard over both lungs. The chest X-ray showed segmental consolidation in right upper lobe, and HRCT showed tubular bronchiectasis and obstruction of right upper lobal bronchus due to mucoid impaction. Allergic skin prick test against Aspergillus fumigatus(Af) and serum precipitin were negative, but intradermal skin test was positive for Af. Specific IgE for Af was positive with class I. Total eosinophil count was 650/mm, total IgE level was more than 3000 IU/ml, ESR was 62mm/hr, and eosinophils in induced sputum was 35%. The tests for sputum acid fast bacilli and sputum cytology for malignancy were negative. The PC20 of bronchial methacholine challenge test was 20.2 mg/ml. Transbronchial lung bi- opsy showed bronchial inflammatory change with infiltration of eosinophils and the existence of fungal hypae. Antibody index for serum IgE-Af and IgG-Af was 10.2 and 2.1 respectively, comparing with Af-sensitive asthma patients.

진균성 심내막염 6예

박영환,조정호,장경희,김준명,김효열,설준희,박윤수,홍성관,최영화,고준상,송영구 대한내과학회 2000 대한내과학회지 Vol.59 No.2

Fungal endocarditis is rare but has been reported with increased frequency in the last few decades. Also fungal endocarditis has become an important infection in the aspect of medical progress and predisposing factors such as previous cardiac surgery, antibiotics use and hyperalimentation, immunosuppression, long-term intravenous catheterization, and drug use. We hereby describe six cases which occurred from January 1992 at Severance hospital, Yonsei University College of Medicine. In five cases infection was associated with previous cardiac surgery and in one case associated with subcutaneous central catheterization in a patient who underwent cancer chemotherapy. Only one patient survived after intensive treatment with fluconazole and surgical removal of vegetation. Others were discharged without improvement of disease or expired during therapy. Fungal endocarditis is still a serious disease with high mortality and whenever the diagnosis is suspected, transesophageal echocardiography should be performed with empirical antifungal therapy. Antifungal therapy and surgery would yield the best results. But overall survival in patient with fungal endocarditis is rather poor. Attentions and efforts for early diagnosis are needed in order to improve the prognosis of fungal endocarditis.(Korean J Med 59:203-207, 2000)

자기공명담관조영술에 의한 간외담관담석의 기원분석

박영수(Young Soo Park),서정훈(Jeong Hun Suh),고준상(Jun Sang Ko),정재복(Jae Bock Chung),김명진(Myung Jin Kim),박승우(Seung Woo Park),이세준(Se Joon Lee),정준표(Jun Pyo Chung),송시영(Si Young Song),강진경(Jin Kyung Kang) 대한내과학회 2002 대한내과학회지 Vol.63 No.3

목적 : 담낭담석과 간외담관담석의 자기공명영상의 특성 및 형태학적인 비교를 통하여 간외담관담석의 을 알아보고자 하였다. 대상 및 방법 : 담석증으로 내원하여 담낭담석과 간외담관담석이 있는 환자를 대상으로 자기공명담관 조영술을 촬영한 38예을 대상으로 하였다. 대상환자는 남자 22명 여자 16명으로 남녀비는 1.38:1이었으며, 연령분포는 30세에서 84세까지 였다(평균 연령 60.7세). 자기공명 담관 조영술은 3명의 연구자가 각각 따로 판독하여 합의를 보았다. 38예 모두에서 담낭담석과 간외담관담석이 각각 외형적 특징과 T2 강조 영상에서의 신호특성에 따라 분석되었다. 담석의 외형적 특징은 난형, 막대기형, 다각형, 모래형, 원형, 혼합형으로 구분 되었고, T2 강조 영상에서의 신호특성은 담석전체에 약한 영상 신호를 보이는 경우를 dark형, 그 외의 불규칙한 영상 신호를 보이는 경우를 heterogeneous형 등 두 가지로 구분하였다. 결과 : 담석의 외형적인 형태에 따른 분류에서 담낭담석 및 간외담관담석의 모양이 동일한 경우는 38예 중 23예(60.5%)였고, 특히 담낭담석 및 간외담관담석에서 모두 난형인 경우는 7예, 다각형인 경우는 10예로 두 경우에 있어는 통계적으로 의미있는 일치도를 보였다. 그리고 간외담관담석의 모양은 난형이 9예, 다각형이 15예, 원형이 6예, 모래형이 4예, 막대형이 1예, 혼합형이 3예에서 관찰되었다. T2 강조 영상에서의 신호특성에 따른 분류에서는 담낭담석과 간외담관담석의 영상신호가 일치한 경우가 38예 중 30예(78.9%)이었다. 이 중 영상 신호가 없는 dark형 24예와 heterogeneous형 6예에서 서로 일치하는 소견을 보였다. 결론 : 담낭담석과 간외담관담석의 자기공명담관 조영술 소견상 담석의 외형적 형태의 일치율이 60.5%로 담낭담석과 간외담관담석이 같이 있는 경우 간외담관담석 중 적어도 약 60%가 담낭담석에서 기인된 이차성 담석임을 추정할 수 있었다. Background : Extrahepatic bile duct stones can be classified into two types. Primary stones are formed de nevo in the bile duct, whereas secondary stones migrate from the gallbladder into the bililary tree. The differentiation between primary and secondary stones is very difficult before operation. The aim of this study is to determine the origin of extrahepatic bile duct stones by analyzing MRC features. Methods : MRC was obtained in 38 patients with stones in both the common duct and gallbladder. MRC findings were compared for gross characteristics and MR signal patterns between common duct stone and gallbladder stone pairs. The gross morphologic characteristics of gallstones on MRC were classified into oval, polygonal, round, rod, sandy and mixed shape. Gallstone patterns seen on heavily T2-weighted images were categorized into one of two patterns: dark, and mixed signal. Results : According to the morphologic characteristics on MRC images, the common duct stones were oval (n=9), polygonal (n=15), round (n=6), sandy (n=4), mixed (n=3) and rod (n=1). The visualized MR signal patterns of common duct stones on heavily T2-weighted images were dark (n=28) and heterogenous (n=10). Conclusion : On MRC, the gross morphologic characteristics and gallstone patterns of common duct stones were similar to those of their paired gallbladder stones in 60.5 to 78.9% of patients. (Korean J Med 63:267-272, 2002)

경피골막증과 동반된 비후성 위염 및 조기 위암 1예

조정호,김진석,이준규,강진경,박인서,문영명,이용찬,민진식,고윤웅,전재윤,고준상 대한소화기내시경학회 2000 Clinical Endoscopy Vol.20 No.1

Pachydermoperiostosis is a rare hereditary syndrome characterized by finger clubbing, periosteal new bone formation of tubular bones, and hypertrophic skin changes (pachydermia). This syndrome is known to be associated with a variety of diseases such as cranial suture defect, female escuchen, bone marrow failure and autonomic nervous system symptoms such as facial flushing and hyperhidrosis. There are just a few reports documenting gastric ulcer, hypertrophic gastropathy and Crohn's disease as associated diseases. A case is herein reported of pachydermoperiostosis accompanied by hypertrophic gastropathy and early gastric cancer.