Flutamide가 수컷 생쥐의 유해감각예민도에 미치는 영향

장세헌,김명정,박제민,양구범,한병득 대한생물치료정신의학회 2000 생물치료정신의학 Vol.6 No.1

Objectives : Previous findings on nociception modulatory effect of testosterone were controversial. In this study, androgen receptor antagonist flutamide was used to reveal testosterone effect on baseline nociceptive sensitivity and stress-induced antinociception in male mice. Methods : Experiments consist 3 Parts: 1) tail flick latency(TFL, sec, 52±1℃) was measured before and 30 minutes after intraperitoneal injection of flutamide 150, 100, 50mg/kg or vehicle(N=15 for each group); 2) TFL was measured before and after serial injection of LHRH antagonist antide(1mg/kg, ip), and 30 minutes after flutamide(100mg/kg, ip) or vehicle; 3) With pretreatment of flutamide(100mg/kg, ip) or vehicle(N=15 for each group), TFL was measured before and after 5 minutes of forced-swim in cold water(15±1℃). Results : 1.TFL was significantly elongated(hypoalgesic) in the flutamide 100 and 150mg/kg groups. TFL of flutamide 150mg/kg group was significantly longer than 50mg/kg group. 2.Though pretreatment with antide itself did not make any change in TFL, it blocked antinociceptive effect of flutamide. TFL was elongated by serial injections in both flutamide and control group. 3.TFL was elongated by FS in both flutamide and control group. FS-induced elongation of TFL was significantly more prominent in flutamide group. Conclusions : From these results, it is suggested flutamide has antinociceptive effect on baseline TFL and potentiates FS- induced antinociception.

전치 태반의 임상적 고찰

홍관표,이성동,김왕수,정종일,장하종,정혁,한세준 朝鮮大學校 附設 醫學硏究所 1991 The Medical Journal of Chosun University Vol.16 No.1

This study was undertaken for the clinical evaluation and statistical analysis on the 38 cases of placenta previa who were admitted and treated from March 1, 1988 to Feb. 28, 1991. The results were as follows : 1. The incidence of placenta previa was one per 53 deliveries or 1.90% 2. There were 57.9 % total placenta previa, 21.1 % partial placenta previa, 13.1 % low-lying placenta previa and 7.9% marginal placenta previa. 3. Placenta previa has occured more often in multipara (63.2%) than primigravida 4. More than one-third (38.8%) cases of placenta previa had delayed admission until labor occured for some time following vaginal spotting has first time been noticed. More instructive prenatal education should be emphasized 5. Lower segment transverse section rate was 63.2%, classical cesarean section rate was 15.8% and cesarean hysterectomy was 9.8%. The rest of them were treated by vaginal deliveries of 5 cases(13.2%) 6. The perinatal loss associated with placenta previa was considered still higher : the premature death was 15.8% and term death was 2.6%. The problem of prevention of prematurity still remains to be studied. 7. There was no maternal mortality in our study

논문발표회 / 제 1 강연장 ( 거동 , 장치 ) : 고압 비증발 조건에서의 원판충돌분무에 관한 연구

(Jang Sae Han),(Seo Kyung Ill),(Cha Keun Jong) 한국액체미립화학회 1995 한국액체미립화학회 학술강연회 논문집 Vol.- No.-

Harmonic Analysis Model based on PSCAD/EMTDC

Han-Min Lee,Chang-Mu Lee,Gil-Soo Jang,Sae-Hyuk Kwon 대한전기학회 2004 KIEE International Transactions on Power Engineeri Vol.a4 No.3

백서 두개골에서 LED 조사가 골 형성에 미치는 영향

Sae WOO Han,Ok Joon Kim,Youn young Jang,Won Bong Lim,Oh Won Mann,Jin Soo Park,Myung Rae Kim,Hong Ran Choi 대한구강악안면병리학회 2004 대한구강악안면병리학회지 Vol.28 No.4

μ방lt emitting diodes (LED) devices are commercially introduced as an alternative for low-Ievellaser therapy (11ι,T) , and it has several advantages over lasers such as a safe, efficient, and less-expensive altemative to treat wounds. And LED irradiation at the same biostimulatory wavelength has similar bíochemical effεαs. In the present study, to asses whether the I핑ht-emitting diode (LED) irradíation can stimulate bone regeneration, irradiated bony defects with or without grafting materials on rat calvaria were compared to corresponding nonirradiated control. Fifty male Sprague-Dawly rats weighing about 150g, were used. Factors for present study were designed as follows, 1) presence or absence of grafting materials, 2) with or without irradiation, and 3) number of irradiation. Two weeks after operation, rats were sacrifìced. Radiologic and 비stomorphologic fmdings were evaluated. Macrospically, there were no incidents of infection, dehiscence, hematoma and necrosis during study. Radiological findings showed greater radiopacity in the graft group and radiopacity increased as the number of irradiation increase. And microscopically, new bαle formation was great in the graft group and increased as the number of irradiation increase, Present study has shown that LED irradiation improved bone regeneration through radiologic and histomorphologic fmdings in rat.

Anti-Tumor Effect of Ginsenoside Rh2 and Rg5 in Xenograft Hepatoma Animal Model: Comparison with Sorafenib

( Han Seul Park ),( Jae Young Jang ),( Soung Won Jeong ),( Jeong-ju Yoo ),( Sae Hwan Lee ),( Sang Gyune Kim ),( Sang-woo Cha ),( Young Seok Kim ),( Young Deok Cho ),( Hong Soo Kim ),( So Young Jin ),( 대한간학회 2018 춘·추계 학술대회 (KASL) Vol.2018 No.1

Aims: Animal & cell models of hepatoma give a crucial information, not only pathogenesis of liver cancer but also therapeutic effects of various agents. In this study, we investigate therapeutic effects of ginsenoside Rh2 and Rg5 using animal & cell models of hepatoma comparing with sorafenib. Methods: Huh7 & huh7.5.1 cells were harvested from 2-D petri-dish and cultured in 1.5 % soft agarose gels for 10-14 days. After 10-14 days, 3-D hepatic structure was formed and treated with ginsenoside Rh2 (100, 200uM) and Rg5 (10, 50, 100uM) for 72h. Hep3b cells (2×10⁵) in matrigel were suspended in 100 μl of phosphate-buffered saline (PBS) and then injected into the flanks of BALB/C nude mouse at 6 weeks. Sorafenib (10mg/kg), ginsenoside Rh2 (100 uM) and Rg5 (100 uM) was injected to intra-peritoneum twice a week. After 4 weeks, all mice were sacrificed and tumor tissue was collected. The tissue was stained with hematoxylin and eosin and histological evaluation was conducted by blindly pathologist. Area of necrosis and vascular change in tumor tissue was calculated using the lasso tool to encircle the area in ZEN 2011 Imaging Software. Results: Both ginsenoside Rh2 and Rg5 induced cell necrosis in hepatocellular carcinoma (HCC) cell lines, and more necrosis occurred in 2D models. The expression of cleaved PARP protein was increased in both 2D and 3D cells with exposure to ginsenoside Rh2 and Rg5. The hepatocellular carcinoma was confirmed in hepatoma mouse models by H&E stain. Increased necrosis and telangiectasia were observed in mice treated with sorafenib, Rh2, and Rg5 compared to control mouse. Conclusions: Our findings provide insight into the use of xenograft mouse as models of HCC and ginsenoside Rh2 & Rg5 might be a potential treatment candidate of liver cancer.

Establishment of Hepatoma Treatment Model Using Hepatoma Cell Spheroids

( Han Seul Park ),( Jae Young Jang ),( Seoung Won Jeong ),( Sae Hwan Lee ),( Sang Gyune Kim ),( Sang-woo Cha ),( Young Seok Kim ),( Young Deok Cho ),( Hong Soo Kim ),( Boo Sung Kim ) 대한간학회 2016 춘·추계 학술대회 (KASL) Vol.2016 No.1

Aims: Three dimensional (3D) spheroid cells are more closely mimic natural tissues and organs than cells grown in 2D. ``Biocompatible spheroidal hepatoma cell`` is thought to be closest model of real patient``s HCC. We have made spheroidal hepatoma cells using the proven technology and already confirmed apoptotic effect of ginsenoside Rh2 and Rg5 on 2D cells (Huh7 and Huh7.5.1). In this study, we investigate apoptotic effects of ginsenoside Rh2 and Rg5 using 3D hepatoma cell spheroids. Methods: Huh7 and Huh7.5.1 cells were maintained in culture dishes in RPMI supplemented with 10% inactivated fetal bovine serum (FBS) and DMEM supplemented with 10 % dialyzed FBS, respectively. When they reached about 80 % confluence, cells were harvested from 2-D petri-dish cultures by treatment with trypsin. These cells cultured in 1.5 % soft agarose gels for 10-14 days. After 10-14 days, 3-D hepatic structure was formed and treated with ginsenoside Rh2 (100, 200uM) and Rg5 (10, 50, 100uM) for 72h. Comparison between 2-D and 3-D models was done with microscopic and protein analysis. Results: The behaviors of 2D and 3D cells (Huh7 and Huh7.5.1, respectively) have been shown different morphologic change. 3-D culture of Huh7 and Huh7.5.1 cells had a longer survival time rather than 2-D cell model. The response to ginsenoside Rh2 and Rg5 on 3D culture systems showed a lower cell death rate compared with 2D culture systems. The expression of cleaved PARP protein was increased in both 2D and 3D cells with exposure to ginsenoside Rh2 and Rg5. Conclusions: Hepatoma cell sheroids had a longer survival time and a similar apoptotic effect compared to 2-D cell model in drug screening. It is expected to have an important role on the drug screening and treatment prediction in HCC.

직장 MALT 림프종에서 전환된 B-미만성 대세포 림프종

강새한,박장원,정병욱,송준규,오현식,박권오 이화여자대학교 의과학연구소 2016 EMJ (Ewha medical journal) Vol.39 No.2

Primary rectal lymphoma is a rare disease among the gastrointestinal (GI) lymphoma. In particular, diffuse large B-cell lymphoma (DLBCL) transformed from mucosaassociated lymphoid tissue (MALT) lymphoma is often the primary type of GI lymphoma, mostly in stomach or duodenum, but has never been reported in rectum. Here we report an unusual case in which a 75-year-old male patient diagnosed with DLBCL transformed from MALT lymphoma in the rectum. The patient was diagnosed as rectal DLBCL transformed from MALT lymphoma as Lugano stage II2 and was treated with chemotherapy (R-CHOP) with CD-20 monoclonal antibody (rituxaimb). Complete remission of multiple lymphadenopathy and mass forming ulcer of the rectum was achieved after 6 cycles of R-CHOP. He has been free from disease for 12 months.

대동맥박리의 임상적 고찰

장병익,박진호,신동구,김영조,심봉섭,이현우,김수현,한승세 영남대학교 의과대학 1992 Yeungnam University Journal of Medicine Vol.9 No.2

저자는 1983년 3월부터 1992년 4월까지 영남대학교 의과대학 부속병원에 입원하여 대동맥박리로 진단받은 34례를 대상으로 이들의 임상상을 관찰한 결과를 요약하면 다음과 같다. 1. 총 대상환자 34명중 남자가 22명, 여자가 12명으로 남녀의 비는 1.83:1로 남자가 많았으며, 호발연령은 60대, 70대, 50대 순이었다. 2. 원인질환으로는 고혈압 및 대동백 경화증이 79%로 대다수를 차지하였다. 3. 증상은 흉하부 혹은 배복부동통이 가장 많아 73%에서 관찰되었고, 이학적 검사상 복부의 박동성종괴가 type Ⅲ 환자 22명중 66%를 차지하였다. 4. 심전도 소견상 심허혈소견이 6명, 심부정맥이 5명, 좌심실비대의 소견이 4명이었으며, 정상심전도의 소견을 가진 예가 16명이었다. 5. 흉부방사선 소견상 대동맥 윤곽이상이 10명에서 관찰되었고, TypeⅢ의 63%에서 정상 흉부방사선소견을 보였다. 6. 가장 많이 사용된 진단 방법은 심 혹은 복부 초음파도로 26명에서 시행되었고, 대동맥조영술은 47%에서 시행되었다. 7. 전체대상환자의 평균사망율은 20%였고 수술사망율은 18%이며, 내과적치료의 적응증이 되어 내과적치료를 받은 환자 중 사망한 례는 없었다. A clinical review of 34 cases of aortic dissection which were admitted to Yeungnam University hospital between March 1983 and April 1992. The results are as follows: 1. The peak incidence was in 5th,6th decade and male to female ratio was 1.83:1. 2. The most common cause of aortic dissection was atherosclerosis and hypertension(79%). 3. The most common presenting symptom was pain(73%), but dyspnea, palpable mass, murmur, shock were also observed. 4. Abnormal electrocardiographic finding was myocardial ischemia in 6 cases, arrythmia in 5 cases, LVH in 5 cases. 5. The X-ray findings showed abnorma aortic conture in 10 cases but normal X-ray finding was observed in 63% of DeBaKey Type Ⅲ. 6. The most common diagnostic procedure was echocardiogram and abdominal ultrasonography. 7. The mortality of all cases was 20 %. operation mortality was 18% but no death of medically treatment in medical indication.

Safe application of reclaimed water reuse for agriculture in Korea

Jang, Taeil,Lee, Sae-Bom,Sung, Choung-Hyun,Lee, Han-Phil,Park, Seung-Woo Springer-Verlag 2010 Paddy and water environment Vol.8 No.3