Phosphorylation of DYNLT1 at Serine 82 Regulates Microtubule Stability and Mitochondrial Permeabilization in Hypoxia

Xue Xu,Yue-sheng Huang,Qiong Zhang,Jiong-yu Hu,Dong-xia Zhang2,Xu-pin Jiang,jie-zhi Jia,Jing-ci Zhu 한국분자세포생물학회 2013 Molecules and cells Vol.36 No.4

Hypoxia-induced microtubule disruption and mitochondrial permeability transition (mPT) are crucial events leading to fatal cell damage and recent studies showed that microtubules (MTs) are involved in the modulation of mitochondrial function. Dynein light chain Tctex-type 1 (DYNLT1) is thought to be associated with MTs and mitochondria. Previously we demonstrated that DYNLT1 knockdown aggravates hypoxia-induced mitochondrial permeabilization, which indicates a role of DYNLT1 in hypoxic cytoprotection. But the underlying regulatory mechanism of DYNLT1 remains illusive. Here we aimed to investigate the phosphorylation alteration of DYNLT1 at serine 82 (S82) in hypoxia (1% O2). We therefore constructed recombinant adenoviruses to generate S82E and S82A mutants, used to transfect H9c2 and HeLa cell lines. Development of hypoxia-induced mPT (MMP examining, Cyt c release and mPT pore opening assay), hypoxic energy metabolism (cellular viability and ATP quantification), and stability of MTs were examined. Our results showed that phosph-S82 (S82-P) expression was increased in early hypoxia; S82E mutation (phosphomimic) aggravated mitochondrial damage, ele-vated the free tubulin in cytoplasm and decreased the cellular viability; S82A mutation (dephosphomimic) seemed to diminish the hypoxia-induced injury. These data suggest that DYNLT1 phosphorylation at S82 is involved in MTs and mitochondria regulation, and their interaction and cooperation contribute to the cellular hypoxic tolerance. Thus, we provide new insights into a DYNLT1 mechanism in stabilizing MTs and mitochondria, and propose a potential therapeutic target for hypoxia cytoprotective studies.

Longitudinal Intrinsic Brain Activity Changes in Cirrhotic Patients before and One Month after Liver Transplantation

Yue Cheng,Li-Xiang Huang,Li Zhang,Ming Ma,Shuang-Shuang Xie,Qian Ji,Xiao-Dong Zhang,Gao-Yan Zhang,Xue-Ning Zhang,Hong-Yan Ni,Wen Shen 대한영상의학회 2017 Korean Journal of Radiology Vol.18 No.2

Objective: To evaluate the spontaneous brain activity alterations in liver transplantation (LT) recipients using resting-state functional MRI. Materials and Methods: Twenty cirrhotic patients as transplant candidates and 25 healthy controls (HCs) were included in this study. All patients repeated the MRI study one month after LT. Amplitude of low-frequency fluctuation (ALFF) values were compared between cirrhotic patients (both pre- and post-LT) and HCs as well as between the pre- and post-LT groups. The relationship between ALFF changes and venous blood ammonia levels and neuropsychological tests were investigated using Pearson’s correlation analysis. Results: In the cirrhotic patients, decreased ALFF in the vision-related regions (left lingual gyrus and calcarine), sensorimotor-related regions (left postcentral gyrus and middle cingulate cortex), and the default-mode network (bilateral precuneus and left inferior parietal lobule) were restored, and the increased ALFF in the temporal and frontal lobe improved in the early period after LT. The ALFF decreases persisted in the right supplementary motor area, inferior parietal lobule, and calcarine. The ALFF changes in the right precuneus were negatively correlated with changes in number connection test-A scores (r = 0.507, p < 0.05). Conclusion: LT improved spontaneous brain activity and the results for associated cognition tests. However, decreased ALFF in some areas persisted, and new-onset abnormal ALFF were possible, indicating that complete cognitive function recovery may need more time.

Magnesium isoglycyrrhizinate inhibits L-type Ca2+ channels, Ca2+ transients, and contractility but not hERG K+ channels

Yue Lin,Yuanyuan Zhang,Qiongtao Song,Tao Song,Xue Han,Ying Zhang,Xuan Zhang,Xi Chu,Fenghua Zhang,Li Chu,Jianping Zhang 대한약학회 2017 Archives of Pharmacal Research Vol.40 No.10

To explore the cardiovascular protective effectsof Magnesium isoglycyrrhizinate (MI), especially theunderlying cellular mechanisms related to L-type calciumchannels and myocardial contractility, and to examine theeffects of MI on hERG K? current expressed in HEK293cells. We used the whole-cell patch clamp technique,video-based edge detection and dual excitation fluorescencephotomultiplier systems to explore the effect of MIon L-type Ca2? currents (ICa-L) and cell contraction in ratcardiomyocytes. We also examined the rapidly activatingdelayed rectifier potassium current (IKr) expressed inHEK293 cells using a perforated patch clamp. MI inhibitedICa-L in a dose-dependent manner, with a half-maximalinhibitory concentration (IC50) of 0.22 mg/ml, and themaximal inhibitory effect was 61.10 ± 0.59%. MI at aconcentration of 0.3 mg/ml reduced cell shortening by24.12 ± 3.97% and the peak value of the Ca2? transient by36.54 ± 4.96%. MI had no significant influence on hERGK? channels expressed in HEK293 cells at all testpotentials. MI exerts protective effects on the heart via theinhibition of ICa-L and cell shortening in rat cardiomyocytes. However, MI had no significant influence on IKr;thus, MI may exert cardioprotective effects without causingdrug-induced long QT syndrome.

Critical effects of long non-coding RNA on fibrosis diseases

Yue Zhang,Gang Luo,Yi Zhang,Mengjie Zhang,Jian Zhou,Weiwu Gao,Xiuyun Xuan,Xia Yang,Di Yang,Zhiqiang Tian,Bing Ni,Jun Tang 생화학분자생물학회 2018 Experimental and molecular medicine Vol.50 No.-

The expression or dysfunction of long non-coding RNAs (lncRNAs) is closely related to various hereditary diseases, autoimmune diseases, metabolic diseases and tumors. LncRNAs were also recently recognized as functional regulators of fibrosis, which is a secondary process in many of these diseases and a primary pathology in fibrosis diseases. We review the latest findings on lncRNAs in fibrosis diseases of the liver, myocardium, kidney, lung and peritoneum. We also discuss the potential of diseaserelated lncRNAs as therapeutic targets for the clinical treatment of human fibrosis diseases.

Design of a Light and Small Dual-band Airborne Despun Optical System

Luqing Zhang,Ning Zhang,Xiping Xu,Kailin Zhang,Yue Zhang,Jiachong Li 한국광학회 2024 Current Optics and Photonics Vol.8 No.1

In aerial cameras, image quality is easily affected by weather, temperature, and the attitude of the aircraft. Aiming at this phenomenon, based on the theory of two-step zoom optical systems, a dual-band optical-despun two-step zoom optical system is designed. The system has a small field of view of 2.00° × 1.60°, and a large field of view of 4.00° × 3.20°. In the zoom process, the wavelength range is 0.45– 0.70 μm and 0.75–1.10 μm, and the size of the optical system is 168 mm (L) × 90 mm (W) × 60 mm (H). The overall lens weight is only 170.8 g, which has advantages for miniaturization and light weight. At the Nyquist frequency of 104 lp/mm, the modulation transfer function of the visible-light optical system is more than 0.44, and that of the near-infrared optical system is more than 0.30, both of which havegood imaging quality and tolerance characteristics in the range of −45 to 60 ℃.

Erratum to: The treatment effect of novel hGHRH homodimer to male infertility hamster

Zhang, Xu-Dong,Guo, Xiao-Yuan,Tang, Jing-Xuan,Yue, Lin-Na,Zhang, Juan-Hui,Liu, Tao,Dong, Yu-Xia,Tang, Song-Shan The Korean Society of Pharmacology 2019 The Korean Journal of Physiology & Pharmacology Vol.23 No.1

Quantification of Ecosystem Carbon Exchange Characteristics in a Dominant Subtropical Evergreen Forest Ecosystem

Yue-Lin Li,Guo-Yi Zhou,De-Qiang Zhang,Katherine Owen Wenigmann,Dennis Otieno,John Tenhunen,Qian-Mei Zhang,Jun-Hua Yan 한국기상학회 2012 Asia-Pacific Journal of Atmospheric Sciences Vol.48 No.1

CO2 fluxes were measured continuously for three years (2003-2005) using the eddy covariance technique for the canopy layer with a height of 27 m above the ground in a dominant subtropical evergreen forest in Dinghushan, South China. By applying gapfilling methods, we quantified the different components of the carbon fluxes (net ecosystem exchange (NEE)), gross primary production (GPP) and ecosystem respiration (Reco) in order to assess the effects of meteorological variables on these fluxes and the atmospherecanopy interactions on the forest carbon cycle. Our results showed that monthly average daily maximum net CO2 exchange of the whole ecosystem varied from −3.79 to −14.24 μmol m−2 s−1 and was linearly related to photosynthetic active radiation. The Dinghushan forest acted as a net carbon sink of −488 g C m−2 y−1, with a GPP of 1448 g Cm−2 y−1, and a Reco of 961 g C m−2 y−1.Using a carboxylase-based model, we compared the predicted fluxes of CO2 with measurements. GPP was modelled as 1443 g C m−2 y−1, and the model inversion results helped to explain ca. 90% of temporal variability of the measured ecosystem fluxes. Contribution of CO2 fluxes in the subtropical forest in the dry season (October-March) was 62.2% of the annual total from the whole forest ecosystem. On average, 43.3%of the net annual carbon sink occurred between October and December, indicating that this time period is an important stage for uptake of CO2 by the forest ecosystem from the atmosphere. Carbon uptake in the evergreen forest ecosystem is an indicator of the interaction of between the atmosphere and the canopy, especially in terms of driving climate factors such as temperature and rainfall events. We found that the Dinghushan evergreen forest is acting as a carbon sink almost year-round. The study can improve the evaluation of the net carbon uptake of tropical monsoon evergreen forest ecosystem in south China region under climate change conditions.

Nonlinear Low Frequency Response Research for a Vibration Isolator with Quasi-Zero Stiffness Characteristic

Yue Zhang,Yufeng Mao,Zhen Wang,Chengfei Gao 대한토목학회 2021 KSCE JOURNAL OF CIVIL ENGINEERING Vol.25 No.5

A vibration isolator system with nonlinear stiffness is designed and tested to solve problems on marine noise measuring equipment which are caused by underwater flow. The low frequency response characteristics of the vibration isolation system are evaluated from static function and dynamic simulation with Harmonic Balance Method. The theoretical analysis results show that the total stiffness value of the isolator system will be kept in 0 point around when parallel springs stay in equilibrium position. In order to assess the practical efficiency of Quasi-Zero-Stiffness (QZS) isolator, a vibration response test is carried out. By comparing the acceleration signals at 5 Hz and 100 Hz between linear and nonlinear system in time domain and frequency domain, the transmissibility curves are obtained. The experimental results demonstrate that the isolation system expands the frequency range of operation effectively by decreasing the natural frequency of the QZS vibration isolator. In addition, the QZS isolation system decreases the transmissibility of vibration by reducing the amplitude of output signals. The QZS isolator has more outstanding performance in contrast to linear spring in ultralow frequency isolation field.

The treatment effect of novel hGHRH homodimer to male infertility hamster

Zhang, Xu-Dong,Guo, Xiao-Yuan,Tang, Jing-Xuan,Yue, Lin-Na,Zhang, Juan-Hui,Liu, Tao,Dong, Yu-Xia,Tang, Song-Shan The Korean Society of Pharmacology 2018 The Korean Journal of Physiology & Pharmacology Vol.22 No.6

Extra-hypothalamic growth hormone-releasing hormone (GHRH) plays an important role in reproduction. To study the treatment effect of Grin (a novel hGHRH homodimer), the infertility models of 85 male Chinese hamsters were established by intraperitoneally injecting 20 mg/kg of cyclophosphamide once in a week for 5 weeks and the treatment with Grin or human menopausal gonadotropin (hMG) as positive control was evaluated by performing a 3-week mating experiment. 2-8 mg/kg of Grin and 200 U/kg of hMG showed similar effect and different pathological characteristics. Compared to the single cyclophosphamide group (0%), the pregnancy rates (H-, M-, L-Grin 26.7, 30.8, 31.3%, and hMG 31.3%) showed significant difference, but there was no difference between the hMG and Grin groups. The single cyclophosphamide group presented loose tubules with pathologic vacuoles and significant TUNEL positive cells. Grin induced less weight of body or testis, compactly aligned tubules with little intra-lumens, whereas hMG caused more weight of body or testis, enlarging tubules with annular clearance. Grin presented a dose-dependent manner or cell differentiation-dependentincrease in testicular GHRH receptor, and did not impact the levels of blood and testicular GH, testosterone. Grin promotes fertility by proliferating and differentiating primitive cells through up-regulating testicular GHRH receptor without triggering GH secretion, which might solve the etiology of oligoasthenozoospermia.

A native Trichoderma harzianum strain Th62 displays antagonistic activities against phytopathogenic fungi and promotes the growth of Celosia cristata

Yue-Feng Wang,Xue-Yue Hou,Chuan-Ying Jiang,Tong-Tong Zhai,Rui Miao,Jun-Jie Deng,Zhi-Hong Yao,Rongshu Zhang 한국원예학회 2021 Horticulture, Environment, and Biotechnology Vol.62 No.2

Trichoderma spp. are widely applied, eco-friendly mycofungicides and plant growth promoters. Native Trichoderma strainsare likely to have more productive, stable biocontrol and biofertilizer activities since they are well adapted to the local environment. In this study, we isolated a native Trichoderma strain ‘Th62’ from the rhizosphere soil of wild Chelidonium majusplants in Harbin, China (126.6341°E, 45.7242°N). The isolated Trichoderma strain was identifi ed as a T. harzianum strainvia morphological observation and molecular methods based on the rDNA internal transcribed spacer region and elongationfactor-1α gene sequences. Signifi cant antagonistic activities of Th62 against fi ve soil-borne fungal phytopathogens,Fusarium oxysporum , Sclerotinia sclerotiorum , Alternaria alternata , Cytospora chrysosperma, and Rhizoctonia solani ,were confi rmed by dual-culture assays. Furthermore, the crude fermentation products of Th62 also displayed antifungalactivities against these fi ve pathogens. To evaluate the function of Th62 as a biofertilizer, we subsequently applied Th62on cockscomb ( Celosia cristata L), a plant species with both ornamental and medicinal values, by inoculation with Th62conidia at diff erent concentrations, 1 × 10 10 cfu mL −1 , 1 × 10 11 cfu mL −1 , and 1 × 10 12 cfu mL −1 . The benefi cial eff ects ofTh62 were evaluated by measuring the growth and photosynthetic traits of the inoculated cockscomb plants, and the resultsdemonstrated that Th62 signifi cantly improved the photosynthetic effi ciency, photosynthetic capacity, and the adaptabilityto intense light of the inoculated cockscomb plants compared to the controls. Consistently, Th62 inoculation signifi cantlyimproved the growth and fl ower yield of cockscomb. We presented a positive case of isolating and applying native microbialresources on local plantation practices.