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( Zhi Ping Zhang ),( Quan Hong Yao ),( Liang Ju Wang ) 생화학분자생물학회 (구 한국생화학분자생물학회) 2010 BMB Reports Vol.43 No.5
5-Aminolevulinate (ALA) is well-known as an essential biosynthetic precursor of all tetrapyrrole compounds, which has been suggested to improve plant salt tolerance by exogenous application. In this work, the gene encoding aminolevulinate synthase (ALA-S) in yeast (Saccharomyces cerevisiae Hem1) was introduced into the genome of Arabidopsis controlled by the Arabidopsis thaliana HemA1 gene promoter. All transgenic lines were able to transcribe the YHem1 gene, especially under light condition. The chimeric protein (YHem1-EGFP) was found co-localizing with the mitochondria in onion epidermal cells. The transgenic Arabidopsis plants could synthesize more endogenous ALA with higher levels of metabolites including chlorophyll and heme. When the T2 homozygous seeds were cultured under NaCl stress, their germination and seedling growth were much better than the wild type. Therefore, introduction of ALA-S gene led to higher level of ALA metabolism with more salt tolerance in higher plants. [BMB reports 2010; 43(5): 330-336]
Zhi-Rong Zhong,Zhi-rong Zhang,Ji Liu,Yong Deng,Hong-wei Zhang,Yao Fu,Qing-guo Song,Qin He 대한약학회 2007 Archives of Pharmacal Research Vol.30 No.1
A novel non-viral gene delivery system, Procationic-Liposome-Protamine-DNA complexes (PLPD) which could further adsorb transferrin on the surface as a targeting ligand to form Tf- PLPD, was prepared and characterized before and after lyophilization. The size distribution of Tf-PLPD was in the range of 240 ± 12 nm and the zeta potential was -24.10 ± 2.5 mV. The transfection efficiencies of PLPD and Tf-PLPD were 12.18 ± 3.8 and 24.26 ± 2.6 mU β-galactosidase/ mg protein respectively. The lyophilization and the presence of serum didn’t affect the tansfectivities of PLPD or Tf-PLPD. Compared to LipofectamineTM 2000 (Invitrogen, U.S.A.), the procationic liposomes had less cytotoxicity to cells. In summary the procationic lipoplex described here, combining the condensing effect of protamine and the targeting capability of Tf, was a perspective non-viral vector for gene delivery system.
Song, Zhi-Yong,Han, Hong-Yan,Zhu, Wei-Yao The Korean Society for Microbiology and Biotechnol 2015 Journal of microbiology and biotechnology Vol.25 No.6
Microbial enhanced oil recovery (MEOR) is being used more widely, and the biological contributions involved in MEOR need to be identified and quantified for the improvement of field applications. Owing to the excellent interfacial activity and the wide distribution of producing strains in oil reservoirs, lipopeptides have proved to be an essential part of the complex mechanisms in MEOR. In this study, crude lipopeptides were produced by a strain isolated from an indigenous community in an oil reservoir. It was found that crude lipopeptides can effectively reduce the IFT (interfacial tension) to 10<sup>-1</sup>~10<sup>-2</sup> mN/m under high salinity without forming stable emulsions, and the wettability of natural sandstone can be enhanced (Amott index, from 0.36 to 0.48). The results of core flooding experiments indicate that an additional 5.2% of original oil in place can be recovered with a 9.5% reduction of injection pressure. After the shut-in period, the wettability of the core, the reduction of injection pressure, and the oil recovery can be improved to 0.63, 16.2% and 9.6%, respectively. In the microscopic flooding experiments, the crude oil in membrane, cluster, and throat states contribute nearly 90% in total of the additional oil recovery, and the recovery of membranestate oil was significantly enhanced by 93.3% after shut in. Based on the results in macro and pore scale, the IFT reduction and the wettability alteration are considered primary contributors to oil recovery, while the latter was more dominant after one shut-in period.
Zhong, Zhi-Rong,Zhang, Zhi-Rong,Liu, Ji,Deng, Yong,Zhang, Hong-Wei,Fu, Yao,Song, Qing-Guo,He, Qin 대한약학회 2007 Archives of Pharmacal Research Vol.30 No.1
A novel non-viral gene delivery system, Procationic-Liposome-Protamine-DNA complexes (PLPD) which could further adsorb transferrin on the surface as a targeting ligand to form Tf-PLPD, was prepared and characterized before and after lyophilization. The size distribution of Tf-PLPD was in the range of $240{\pm}12nm$ and the zeta potential was $-24.10{\pm}2.5mV$. The transfection efficiencies of PLPD and Tf-PLPD were $12.18{\pm}3.8\;and\;24.26{\pm}2.6mU\;{\beta}-galactosidase/mg$ protein respectively. The lyophilization and the presence of serum didn't affect the tansfectivities of PLPD or Tf-PLPD. Compared to $Lipofectamine^{TM}$ 2000 (Invitrogen, U.S.A.), the procationic liposomes had less cytotoxicity to cells. In summary the procationic lipoplex described here, combining the condensing effect of protamine and the targeting capability of Tf, was a perspective non-viral vector for gene delivery system.
Yang, Zhi-Ping,Xie, Yong-Hong,Ling, Dan-Yan,Li, Jin-Rui,Jiang, Jin,Fan, Yao-Hua,Zheng, Jia-Lian,Wu, Wan-Xin Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.17
SCY1-like 1-binding protein 1 (SCYL1BP1) is a newly identified transcriptional activator domain containing protein with many unknown biological functions. Recently emerging evidence has revealed that it is a novel regulator of the p53 pathway, which is very important for the development of human cancer. However, the effects of SCYL1BP1 on human lung squamous carcinoma cell biological behavior remain poorly understood. In this study, we present evidence that SCYL1BP1 can promote the degradation of MDM2 protein and further inhibit the G1/S transition of lung squamous carcinoma cell lines. Functional assays found that reintroduction of SCYL1BP1 into lung squamous carcinoma cell lines significantly inhibited cell proliferation, migration, invasion and tumor formation in nude mice, suggesting strong tumor suppressive function of SCYL1BP1 in lung squamous carcinoma. Taken together, our data suggest that the interaction of SCYL1BP1/MDM2 could accelerate MDM2 degradation, and may function as an important tumor suppressor in lung squamous carcinomas.
중국 배달 앱의 온라인리뷰가 행동의도에 미치는 영향에 관한 연구 -가격민감도와 멤버십의 매개적 조절효과-
지로요 ( Zhi Lu Yao ),하홍열 ( Ha Hong Youl ) 한중사회과학학회 2022 한중사회과학연구 Vol.20 No.1
본 연구는 배달 앱을 이용하는 중국 소비자를 대상으로, 온라인리뷰가 어떻게 소비자의 행동의도에 영향을 미치는가를 고찰한다. 이를 위해 온라인 고객이 상품을 구매할 때 고려하는 가격민감도와 특정 앱을 이용할 때 사용되는 고객정보인 멤버십의 조절 및 매개적 조절효과를 함께 고찰한다. 총 881명의 유효한 표본을 가지고, 본 연구에서 제시한 가설을 회귀분석 Process Macro를 이용하여 분석하였다. 연구결과를 살펴보면, 첫째, 온라인리뷰 수준이 높아지면, 소비자의 지각된 가치에 긍정적인 영향을 준다는 결과를 보여주고 있다. 둘째, 온라인리뷰와 행동의도의 관계는 가격민감도 수준에 의해 영향을 받고 있으며, 특히, 가격민감도가 낮은 집단에서는 온라인리뷰가 긍정적일 때, 행동의도가 높아진다. 다만, 가격민감도가 높은 집단에서 온라인리뷰가 긍정적일 때, 행동의도는 상대적으로 높아진다. 마지막으로, 가격민감도가 높고 비회원인 고객인 경우, 온라인리뷰가 긍정적일 때, 지각된 가치가 상대적으로 낮게 나타나는 결과를 보여주고 있다. This study examines how online reviews influence behavioral intentions in the context of the Chinese food delivery applications. In so doing, we test the impacts of price sensitivity and the type of customer membership using the mediated moderation approach which plays an important role in bridging the relationship between the two constructs. We collected a total of 881 usable samples, and then, test our proposed hypotheses using Process Macro. First, our findings show that positive online reviews significantly influence customers’ perceived value. Second, the relationship between online reviews and behavioral intentions is affected by the level of price sensitivity. In particular, for the group of low price sensitivity, when online reviews are positive, behavioral intentions are high. However, for the group of high price sensitivity, when online reviews are positive, behavioral intentions are relatively high. Finally, in the case of high price sensitivity and non-membership customers, when online reviews are positive, perceived value is relatively low.
Bioactive Cadinane-type Compounds from the Soft Coral Sinularia scabra
Jyh-Horng Sheu,Jui-Hsin Su,Chiung-Yao Huang,Po-Ju Li,Yi Lu,Zhi-Hong Wen,Yao-Haur Kao 대한약학회 2012 Archives of Pharmacal Research Vol.35 No.5
Two new cadinane-type sesquiterpenoids, scabralins A (1) and B (2) were obtained from the soft coral Sinularia scabra. Metabolite 1 was shown to exhibit moderate to weak cytotoxicity against MCF-7, WiDr, Daoy, and HEp 2 cancer cell lines. Also, incubation with 10 μM compound 1 significantly inhibited the accumulation of the pro-inflammatory inducible nitric oxide synthase protein in lipopolysaccharide-stimulated RAW264.7 macrophage cells.