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Yue Lin,Yuanyuan Zhang,Qiongtao Song,Tao Song,Xue Han,Ying Zhang,Xuan Zhang,Xi Chu,Fenghua Zhang,Li Chu,Jianping Zhang 대한약학회 2017 Archives of Pharmacal Research Vol.40 No.10
To explore the cardiovascular protective effectsof Magnesium isoglycyrrhizinate (MI), especially theunderlying cellular mechanisms related to L-type calciumchannels and myocardial contractility, and to examine theeffects of MI on hERG K? current expressed in HEK293cells. We used the whole-cell patch clamp technique,video-based edge detection and dual excitation fluorescencephotomultiplier systems to explore the effect of MIon L-type Ca2? currents (ICa-L) and cell contraction in ratcardiomyocytes. We also examined the rapidly activatingdelayed rectifier potassium current (IKr) expressed inHEK293 cells using a perforated patch clamp. MI inhibitedICa-L in a dose-dependent manner, with a half-maximalinhibitory concentration (IC50) of 0.22 mg/ml, and themaximal inhibitory effect was 61.10 ± 0.59%. MI at aconcentration of 0.3 mg/ml reduced cell shortening by24.12 ± 3.97% and the peak value of the Ca2? transient by36.54 ± 4.96%. MI had no significant influence on hERGK? channels expressed in HEK293 cells at all testpotentials. MI exerts protective effects on the heart via theinhibition of ICa-L and cell shortening in rat cardiomyocytes. However, MI had no significant influence on IKr;thus, MI may exert cardioprotective effects without causingdrug-induced long QT syndrome.
A Self-Sampling-and-Flow Biosensor for Continuous Monitoring
Zhang, Fenghua,Kim, Jong ,Sik,Cui, Gang,Cha, Geun ,Sig,Nam, Hakhyun WILEY-VCH Verlag 2005 Electroanalysis Vol.17 No.8
<P>A self-sampling-and-flow biosensor was fabricated by sandwiching a nitrocellulose strip on the working electrode side of the double-sided microporous gold electrodes and a wicking pad on the counter electrode side. The double-sided microporous electrodes were formed by plasma sputtering of gold on a porous nylon substrate. Sample was taken up to the enzyme-immobilized working electrode by the capillary action of the front nitrocellulose strip dipped into the sample solution, analyzed electrochemically at the enzyme-immobilized electrode, and diffuses out to the backside wicking pad through the micropores of the electrodes, constituting a complete flow cell device with no mechanical liquid-transporting device. Biosensor was formed by co-immobilizing the glucose oxidase and electron transfer mediator (ferrocene acetic acid) on the thioctic acid self-assembled monolayer-modified working electrode. A typical response time of the biosensor was about 5 min with the sensitivity of 2.98 nA/mM glucose, providing linear response up to 22.5 mM. To demonstrate the use of self-sampling-and-flow biosensor, the consumption rate of glucose in the presence of yeast was monitored for five days. </P>
Gold Nanoparticle-Based Mediatorless Biosensor Prepared on Microporous Electrode
Zhang, Fenghua,Cho, Soon ,Sam,Yang, Soon ,Hye,Seo, Seong ,Suk,Cha, Geun ,Sig,Nam, Hakhyun WILEY-VCH Verlag 2006 Electroanalysis Vol.18 No.3
<P>A mediatorless biosensor was fabricated with a double-sided microporous gold electrode by successively immobilizing a mixed self-assembled monolayer (SAM) comprising carboxylic-acid- and thiol-terminated thiolate (dl-thiorphan and 1,8-octanedithiol), glucose oxidase (GOx) and finally gold nanoparticle (Au NP) on one working side. The double-sided microporous gold electrodes were formed by plasma sputtering of gold on a porous nylon substrate, yielding a face-to-face type two-electrode electrochemical cell. While the straight chain molecule 1,8-octanedithiol forms a dense insulating monolayer, the side armed dl-thiorphan forms a low density layer for the diffusion of redox couples to the electrode surface. The mixed SAM not only provided the linking functional groups for both enzyme and Au NP but also resulted in the appropriately spaced monolayer for direct electron tansfer (ET) process from the center of the redox enzyme to the electrode surface. After covalently immobilizing GOx onto the carboxylic-acid-terminated monolayer, Au NP was easily immobilized to both enzyme and nearby thiols by simple dispensing of the colloidal gold solution. It was observed that the resulting amperometric biosensor exhibited quantitatively the same response to glucose in the presence and in the absence of dissolved oxygen, which evidence that the Au NPs immobilized on and around the GOx promote direct ET from the enzymes to the electrode, assuming the role of a common redox mediator.</P>
Attribute-Based Data Sharing with Flexible and Direct Revocation in Cloud Computing
( Yinghui Zhang ),( Xiaofeng Chen ),( Jin Li ),( Hui Li ),( Fenghua Li ) 한국인터넷정보학회 2014 KSII Transactions on Internet and Information Syst Vol.8 No.11
Attribute-based encryption (ABE) is a promising cryptographic primitive for implementing fine-grained data sharing in cloud computing. However, before ABE can be widely deployed in practical cloud storage systems, a challenging issue with regard to attributes and user revocation has to be addressed. To our knowledge, most of the existing ABE schemes fail to support flexible and direct revocation owing to the burdensome update of attribute secret keys and all the ciphertexts. Aiming at tackling the challenge above, we formalize the notion of ciphertext-policy ABE supporting flexible and direct revocation (FDR-CP-ABE), and present a concrete construction. The proposed scheme supports direct attribute and user revocation. To achieve this goal, we introduce an auxiliary function to determine the ciphertexts involved in revocation events, and then only update these involved ciphertexts by adopting the technique of broadcast encryption. Furthermore, our construction is proven secure in the standard model. Theoretical analysis and experimental results indicate that FDR-CP-ABE outperforms the previous revocation-related methods.
Kaiyun Qin,Fenghua Zhang,Hongxia Wang,Na Wang,Hongbing Qiu,Xinzhuan Jia,Shan Gong,Zhengmao Zhang 생화학분자생물학회 2023 BMB Reports Vol.56 No.3
Ovarian cancer (OC) is the most common gynecological malignancyworldwide, and chemoresistance occurs in most patients,resulting in treatment failure. A better understanding of themolecular processes underlying drug resistance is crucial fordevelopment of efficient therapies to improve OC patient outcomes. Circular RNAs (circRNAs) and ferroptosis play crucialroles in tumorigenesis and resistance to chemotherapy. However,little is known about the role(s) of circRNAs in regulatingferroptosis in OC. To gain insights into cisplatin resistance inOC, we studied the ferroptosis-associated circRNA circSnx12. We evaluated circSnx12 expression in OC cell lines and tissuesthat were susceptible or resistant to cisplatin using quantitativereal-time PCR. We also conducted in vitro and in vivoassays examining the function and mechanism of lnc-LBCSs. Knockdown of circSnx12 rendered cisplatin-resistant OC cellsmore sensitive to cisplatin in vitro and in vivo by activatingferroptosis, which was at least partially abolished by downregulationof miR-194-5p. Molecular mechanics studies indicatethat circSnx12 can be a molecular sponge of miR-194-5p, whichtargets SLC7A11. According to our findings, circSnx12 amelioratescisplatin resistance by blocking ferroptosis via a miR-194-5p/SLC7A11 pathway. CircARNT2 may thus serve as an effectivetherapeutic target for overcoming cisplatin resistance in OC.
Huang Liang,Bai Fenghua,Zhang Yutian,Zhang Shanshan,Jin Tianbo,Wei Xingwei,Zhou Xiaoli,Lin Mei,Xie Yufei,He Chanyi,Lin Qi,Xie Tian,Ding Yipeng 한국유전학회 2022 Genes & Genomics Vol.44 No.8
Background: Thyroid hormones are critical regulators of metabolism, development and growth in mammals. However, the genetic association of thyroid-related hormones in the Chinese Han population is not fully understood. Objective: We aimed to identify the genetic loci associated with circulating thyroid-related hormones concentrations in the healthy Chinese Han population. Methods: Genotyping was performed in 124 individuals using Applied Biosystems™ Axiom™ PMDA, and 796,288 single nucleotide polymorphisms (SNPs) were available for the GWAS analysis. For replication, eleven SNPs were selected as candidate loci for genotyping by Agena MassARRAY platform in additional samples (313 subjects). The values of p < 5 × 10- 6 suggest a suggestively significant genome-wide association with circulating thyroid-related hormones concentrations. Results: We identified that rs11178277 (PTPRB, p = 4.88 × 10- 07) and rs7320337 (LMO7DN-KCTD12, p = 1.22 × 10- 06) were associated with serum FT3 level. Three SNPs (rs4850041 in LOC105373394-LINC01249: p = 3.55 × 10- 06, rs6867291 in LINC02208: p = 2.40 × 10- 06 and rs79508321 in WWOX: p = 3.35 × 10- 06) were related to circulating T3 level. Rs12474167 (LOC105373394-LINC01249, p = 1.65 × 10- 06) and rs1864553 (IWS1, p = 2.00 × 10- 06) were associated with circulating T4 concentration. The association with TGA concentration was for rs17163542 in DISP1 (p = 3.46 × 10- 06) and rs12601151 in NOG-C17orf67 (p = 2.72 × 10- 07). Two genome-level significant SNPs (rs2114707 in LINC01314, p = 1.69 × 10- 06 and rs12601151, p = 1.41 × 10- 07) associated with serum TMA concentration were identified. Moreover, rs6083269 (CST1-CST2, p = 3.36 × 10- 06) was a significant locus for circulating TSH level. In replication, rs12601151 in NOG-C17orf67 was still associated with serum TGA level (p = 0.012). Conclusions: The GWAS reported 11 new suggestively significant loci associated with circulating thyroid-related hormones levels among the Chinese Han population. These findings represented suggestively biological candidates for circulating thyroid-related hormones levels and provided new insights into the mechanisms of regulating serum TGA concentration.
Bo Wu,Peng Li,Fenghua Zhang,Feng Tian 대한기계학회 2021 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.35 No.5
In this study, an orthotropic plate arranged at the bottom of the phase change material (PCM) -based heat sink is employed to enhance the temperature field uniformity (TFU) in the heat sink. The performance of the orthotropic plate in enhancing the TFU is numerically studied during the melting process of the PCM. The effects of the orthotropic conductivity (R) are examined at R = 10 Rb, 5 Rb, 0.2 Rb and 0.1 Rb. The configuration including a copper plate is also taken into consideration as a contrast to achieve a more objective and deeper insight. It is found that the orthotropic plate can significantly enhance the TFU by up to 4 times in the PCM regions; it can also narrow down the temperature range in the heat sink region. When the orthotropic conductivity is greater than Rb, it can even benefit in improving the performance of thermal management of the heat sink. Principles about the orthotropic conductivity are drawn and comprehensive evaluations are conducted to guide in designing PCM-based heat sink for thermal management of avionics.